Supplementary material
Roles of catalase and glutathione peroxidase in the tolerance of a
pulmonate gastropod to anoxia and reoxygenation
Alexis F. Welker1,2, Daniel C. Moreira1 and Marcelo Hermes-Lima1,*
1Laboratório de Radicais Livres, Departamento de Biologia Celular, Universidade de Brasília,
Brasília, DF, 70910-900, Brazil.
2Faculdade da Ceilândia, Universidade de Brasília, Ceilândia, DF, 72220-140, Brazil.
*Corresponding author:
Dr. Marcelo Hermes-Lima
Laboratório de Radicais Livres, Departamento de Biologia Celular, Universidade de Brasília, Brasília,
DF, 70910-900, Brazil.
Phone: +55-61-3107-3103
Email: [email protected] , [email protected] and [email protected]
Fig. S1 Effects of ATZ injection and reoxygenation on total glutathione peroxidase (total-GPX) activity (mU/mg of protein),
glutathione transferase (GST) activity (U/mg of protein) and total glutathione equivalents (GSH-eq) levels (nmol/g of wet
weight) in hepatopancreas (a, b, c) and foot muscle (d, e, f) of Helix aspersa snails. Activity of total GPX (a, d) and GST (b,
e) and GSH-eq levels (c, f) in hepatopancreas and foot muscle from saline- or ATZ-injected snails exposed to anoxia and at
different reoxygenation times “zero” (5 h anoxia), 0.25 h, 0.5 h, 1 h, 2 h and 12 h from saline-injected and ATZ-injected snails.
There were no statistically significant alterations. (n=5–6)
Fig. S2 Effects of anoxia and ATZ injection (a,c) and of reoxygenation (b,d) with catalase inhibition
or not on reduced glutathione (GSH) levels (nmol/g of wet weight) of Helix aspersa snails. GSH
concentration in hepatopancreas (a) and foot muscle (c) from control (Ctrl, no injection), saline- (Sal,
animals injected with 0.9% (w/v) NaCl) or ATZ-injected (ATZ, animals injected with ATZ at 1
mg/gww solubilized in 0.9% NaCl) snails maintained in normoxia or exposed to anoxia. GSH
concentration in hepatopancreas (b) and foot muscle (d) at different reoxygenation times “zero” (5 h
anoxia), 0.25 h, 0.5 h, 1 h, 2 h and 12 h from saline-injected and ATZ-injected snails. There were no
statistically significant alterations. (n=5–6)
Fig. S3 Effects of anoxia and ATZ injection (a,c) and of reoxygenation (b,d) with catalase inhibition
or not on disulfide glutathione (GSSG) levels (nmol/g of wet weight) of Helix aspersa snails. GSSG
concentration in hepatopancreas (a) and foot muscle (c) from control (Ctrl, no injection), saline- (Sal,
animals injected with 0.9% (w/v) NaCl) or ATZ-injected (ATZ, animals injected with ATZ at 1
mg/gww solubilized in 0.9% NaCl) snails maintained in normoxia or exposed to anoxia. GSSG
concentration in hepatopancreas (b) and foot muscle (d) at different reoxygenation times “zero” (5 h
anoxia), 0.25 h, 0.5 h, 1 h, 2 h and 12 h from saline-injected and ATZ-injected snails. There were no
statistically significant alterations. (n=5–6)