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Self-Powered Biosensor for Ascorbic Acid with a Prussian Blue Electrochromic Display
Adrianna ZloczewskaAnna CelebanskaKatarzyna SzotDorota TomaszewskaMarcin OpalloMartin Jönsson-Niedziółka
Institute of Physical ChemistryPolish Academy of SciencesWarsaw, Poland
Martin Jönsson-Niedziólka, [email protected] Twitter: @ekorrhjulet
Mikrosympozjum sprawozdawcze, IPS PAS.
Institute of Physical Chemistry PAS, Warsaw Poland
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Outline
- Self-powered sensors- CNP electrodes- Ascorbic acid sensing- Biocathode- Ascorbic acid biofuel cell- Self-powered sensor
Photo: Gabriel Hess
Institute of Physical Chemistry PAS, Warsaw Poland
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Self-powered sensor
Fuel cell where output is determined by concentration of analyteE. Katz, A. F. Bückmann, and I. Willner, J. Am. Chem. Soc. 123, 10752 (2001).
Institute of Physical Chemistry PAS, Warsaw Poland
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Truly self-powered sensors
Foto: Jessica Bender
T. Hanashi, T. Yamazaki, W. Tsugawa, S. Ferri, D. Nakayama, M. Tomiyama, K. Ikebukuro, and K. Sode, Biosensors & Bioelectronics 24, 1837 (2009).
BioCapacitor
0.1 mM glucose
1.1 mM glucose
Institute of Physical Chemistry PAS, Warsaw Poland
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Modification of ITO electrodes with carbon nanoparticles
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CNP- solutiona
q
CNP+ solutiona
q
+ carbon nanoparticle modified with positively charged ammonium functionalities
SO
O
N H
NH3+
SO3 carbon nanoparticle modified with phenylsulfonate
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J.D. Watkins et al. Phys.Chem.Chem.Phys., 12 4872 (2010). K. Szot, et al., Electrochem. Commun. 12, 737 (2010).
1 layer
3 layers
Institute of Physical Chemistry PAS, Warsaw Poland
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Ascorbic acid oxidation
CNP+-CNP- electrode
ITO
M. Kundys et al., Anal. Methods 6 (2014) 7532.
Institute of Physical Chemistry PAS, Warsaw Poland
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Ascorbic acid sensing
Cyclic voltammetry on CNP film electrode in phosphate buffer, pH = 4.8 (dashed line) and in AA solutions of different concentrations: 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0 and 6.0 mM; v = 10 mVs-1
A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.
CNP+-CNP- electrode
Institute of Physical Chemistry PAS, Warsaw Poland
9A. Zloczewska, M Jönsson-Niedziolka, J. Power Sources 228, 104-111 (2013).
RE
CE
WE
Cu tape
silicone spacer
UV-Vis cuvettes with holes
electrolyte
H2O
H+
O2 gas-side
electrolyte-
side
electrolyte
ē
RE – reference electrodeCE - counter electrode
SWCNT – single-walled CNTs
Bilirubin oxidase
Functionalised SWCNT
Silicate matrix
Biocathode
Institute of Physical Chemistry PAS, Warsaw Poland
10A. Zloczewska, M Jönsson-Niedziolka, J. Power Sources 228, 104-111 (2013).
H2O
H+
O2 gas-side
electrolyte-
side
electrolyte
ē
RE – reference electrodeCE - counter electrode
SWCNT – single-walled CNTs
Bilirubin oxidase
Functionalised SWCNT
Silicate matrix
Biocathode
Gas-side filled with: argon a) & b), air c) and dioxygen d). a) the electrolyte was also deaerated.
a)
b)
c)
d)
Institute of Physical Chemistry PAS, Warsaw Poland
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Ascorbic acid BFC
AA-anode and the biocathode working in 1 mM AA,
A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.
Institute of Physical Chemistry PAS, Warsaw Poland
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Ascorbic acid BFC
AA concentrations: 1, 2, 4 and 6 mM.
CNP+-CNP- anode - Air-breathing CNT cathode
A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.
Institute of Physical Chemistry PAS, Warsaw Poland
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Ascorbic acid BFC
CNP+-CNP- anode - Air-breathing CNT cathode
AA-BFC working as a Willner-Katz type self-powered sensor.
CNP anode | CNT air-breathing cathodeCurrent measured at constant E = 0.3V.
A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.
Institute of Physical Chemistry PAS, Warsaw Poland
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Electrochromic display
Prussian Blue display as sensor output. Display prepared by electro-deposition of PB on ITO.
J. J. García-Jareño, D. Benito, J. Navarro-Laboulais, F. Vicente, and J. Garcia-Jareno, J. Chem. Educ. 75, 881 (1998).
Institute of Physical Chemistry PAS, Warsaw Poland
https://crystallography365.wordpress.com/2014/02/02/are-you-feeling-blue-the-structure-of-prussian-blue/
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Truly self-powered sensor
H. Liu and R. M. Crooks, Analytical Chemistry 84, 2528 (2012).
A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.
Institute of Physical Chemistry PAS, Warsaw Poland
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Higher accuracy using image analysis
J. L. Delaney, C. F. Hogan, J. Tian, and W. Shen, Analytical Chemistry 83, 1300 (2011).
Automated analysis of colour of photos taken each 5 s.
Transparency values fitted to find colour after 60 s
A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.
Institute of Physical Chemistry PAS, Warsaw Poland
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Conclusions
We have used carbon nanoparticle electrodes for AA oxidation a low potential.
We can connect these anode to a biocathode to made an AA biofuel cell
The AA BFC can be used a self-powered sensor
It can also be connected to a PB display to make a truly self-powered sensor.
Institute of Physical Chemistry PAS, Warsaw Poland
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Acknowledgements
This work was partially financed by:
The European Union 7.FP under the grant REGPOT-CT-2011- 285949-NOBLESSE
The Polish Ministry of Science and Higher Education under the contract IP2011 020771.
The work of AZ was realized within the International PhD Projects Programme of the Foundation for Polish Science, co-financed from the European Regional Development Fund within the Innovative Economy Operational Programme "Grants for innovation"
Thank you for your attention
NanOtechnology, Biomaterials and aLternative Energy Source for ERA integration
FP7–REGPOT–CT–2011–285949-NOBLESSE
Institute of Physical Chemistry PAS, Warsaw Poland