PROGRESS REPORT
UNDER STAR COLLEGE SCHEME
Dept. of Biotechnology
Govt. of India
2012-2015
St. Agnes College (Autonomous)
Mangaluru
Reaccredited with “A” grade by NAAC (2012)
College with Potential for Excellence 2006
DEPARTMENT OF BOTANY
FACULTY & COMBINATION
Dr. Noeline J. Pinto
Ph. D, B.Ed.
Minor Research Projects (2) Completed Guided one M.Phil dissertation
Dr. Maria G. Lobo
Ph. D., PDF – (USA) Minor Research Projects -2 Completed - 1, ongoing -1
Sl No Activities conducted Number
1 New Practicals 07
2 Student’s Projects 20
3 Hands-on Training Programs 02
4 Paper Presentations 07
5 Field visits/ Educational tours 04/02
6 Seminars 03
7 Guest Lectures / Invited Speakers 02
8 Extension / other Activities 04
LIST OF ACTIVITIES
Sl. No. Equipment Name (Model no.)
1 Horizontal Submarine Electrophoresis System 106470
2 Electric Power Supply 106856
3 Digital PH meter 335
4 Electronic Balance GE412
5 Table Top Centrifuge R-8C
6 UV Transilluminator with filter 107171
7 Microcontroller based visible Spectrophotometer
8 Visible Spectrophotometer - Model 166
9 Microcontroller based Flame Photometer
10 Horizontal Electrophoresis (10 x 7)
11 Canon camera with lens & Accessories
12. Tilting Shaker Model RTS-1
13. Digital Colorimeter
EQUIPMENT PROCURED FOR THE
PROPOSED NEW PRACTICALS
TRI ACID DIGESTION
NEW INSTRUMENTS HANDLED
MUFFLE FURNACE
SHAKER FLAME PHOTOMETER SPECTROPHOTOMETER
SOXHLET TRI ACID DIGESTION
LIST OF NEW PRACTICAL'S
I B. Sc. II B. Sc. III B. Sc.
1. Bacterial and fungal culture techniques
1. In-vitro pollen germination and micrometry to measure the length of pollen tubes.
1. Determination of alkalinity & Phosphate content of water using colorimeter.
2. Haemocytometer to calculate phytoplankton density.
3. DNA extraction from coconut endosperm.
4. Agarose/Polyacrylamide gel electrophoresis.
5. Plant tissue culture – Callus induction, Anther culture.
NEW / IMPROVEMENT IN THE EXISTING PRACTICALS
1. Culture techniques
• Earlier/existing :
Bacterial culture plates from microbiology used as
spotters
• Improvement:
Culture media for Bacteria and Fungi. Preparation
of media, culture plate and inoculation / Culture technique
2. Gram Staining of Bacteria
• Earlier/existing:
Observation of prepared slides
• Improvement:
Students individually test and identify gram +ve and –ve bacteria from their cultures.
I B.Sc.
I
1. In-vitro pollen germination and micrometry to measure the length of pollen tubes
Earlier/existing:
Demonstration by the teacher and observation by
student
Improvement:
Micrometers provided to students individually, so they can perform independently.
2. Digital record of plants
Earlier/existing:
Submission of herbaria only
Improvement: Students use digital camera to photograph Angiosperm Plants in the wild, label them and Submit for the examination. Learn the use of technology.
II B.Sc.
1. Physiology experiments – a. Ganong’s potometer;
b. Ganong’s respirometer;
c. Kuhne’s fermentation apparatus;
d. Thistle funnels;
e. Hydrotropism;
f. Garreau’s potometer;
g. Chlorophyll separation – hair driers
Existing: Limited apparatus so experiments performed in groups Improvement: With individual apparatus the student is confident to perform the experiment.
III B.Sc.
2. Estimation of Carbohydrates and Proteins
Existing: Limited instruments/Glassware so experiments performed in groups. Improvement: With additional instruments students perform the experiments are individually.
3. Isolation of DNA from Coconut Endosperm
Existing: Not a part of the syllabus Improvement: Training students in the technique of DNA isolation
4. Determination of
alkalinity & Phosphate content of water using colorimeter
Existing: Not a part of the syllabus Improvement: Students learn the ecological relevance in estimation of alkalinity & phosphates.
5. Haemocytometer to
calculate phytoplankton density.
Existing: Not a part of the syllabus Improvement: Students learn the ecological relevance in estimation of phytoplanktons
6. Gel Electrophoresis – Horizontal & Vertical
Existing: Not a part of the syllabus Improvement: Students learn gel casting, loading & gel running to see the illuminated bands
7. Plant Tissue Culture Existing: Not a part of the syllabus Improvement: Students get ‘Hands-On’ training and learn types of tissue culture
Exposure to small research projects based on some practicals.
Trained in literature survey, report writing, designing suitable experiments, handling new instruments.
Opportunity to attend Seminars, present papers and aim to obtain best awards.
INTERDISCIPLINARY / INTERDEPARTMENTAL
PROJECTS EXECUTED BY THE STUDENTS
PROJECT TITLE -2012 -13 No. of
Students
1 Nutritional Profile of Dry fruits (Interdisciplinary- With Chemistry) 3
2 Nutritional aspects of Edible Mushrooms (Interdisciplinary - With Chemistry)
3
3 Antimicrobial activity of Acmella oleracea 3
4 Diversity of Ferns of Dakshina kannada/ Western Ghats. ( Contd. 3 years)
2
Antimicrobial activity Analysis of Carbohydrates and Proteins
STUDENT’S PROJECTS 2013-14
Sl
No Project Title
No. of
Students
1 Estimation of Protein from Grapes, Tomato, Pomeogranate & Apple 2
2 Estimation of Chlorophylls from Murraya, Melia, Moringa & Trigonella
leaves.
2
3 Determination of titrable acid number from Grapes, Tomato, Orange
water melon, Apple and Pomeogranate
3
4 Estimation of Protein and Carbohydrates of Cassia tora leaves 1
5 Estimation of Protein and Carbohydrates of Cassia tora seeds 1
6 Estimation of Protein and Carbohydrates of Anethum graveolens 1
7 Estimation and extraction of Lycopene from Tomato 2
8 Estimation and extraction of Lycopene from Red Capsicum 2
9 Estimation and extraction of Lycopene from Watermelon 2
10 Antimicrobial activity of Vernonia antihelmintica 3
Extraction of Lycopene from Watermelon
Drumstick Methi Murraya Neem
1.1
05
1.2
57
0.6
85
1.4
61
0.5
55
0.6
21
0.3
94
0.7
92
1.6
6
1.8
78
1.0
79
2.2
53
Chlorophyll a Chlorophyll b Total Chlorophyll
Extraction of Chlorophyll
Determination of TAN
STUDENT’S PROJECTS 2014 -15
Project Title No. of
Students
1 Estimation of Protein and Proline of selected Pulses 3
2 Estimation of Chlorophylls and Carotenoids of
Sauropus, Coleus & Margosa
3
3 Estimation of Tannins Tea, Amla & Amchur 3
4 Estimation of Phenols from selected fruits. 3
5 Estimation of Free fatty acids from edible oils and fats 3
6 Study of Bacterial population in the Campus 3
PAPER PRESENTATIONS 2012 - 2013 2013 - 2014 2014 - 2015
1. Comparative Study of
Protein & Carbohydrate
contents of five Dry
Fruits
4. “Study on Diversity
of Ferns of Dakshina
Kannada”
5 . Nutritional Profile of
wild edible
Musrhrooms . ( Won II place
2. Nutritional Profile of
Mushrooms – A Study
6 . Antimicrobial Activity
of Vernonia antihelmintica
3. Antimicrobial Activity of
Acmella oleracea - ( I Place)
7. Nutrient analysis of
Cassia tora leaves and seeds
• Seminar on ‘Challenges and Prospects of Basic Sciences’ September 1st – 2012.
• National Level Seminar on ‘Emerging Trends in Biological sciences’ on January 18- 19, 2013 – Biological sciences.
• Workshop on “Science in Action”– January, 8 _10, 2015
WORKSHOPS AND SEMINARS ORGANIZED IN TOPICAL AREAS FOR STUDENTS BY THE DEPARTMENTS
Seminar on ‘Challenges and Prospects of
Basic Sciences’ September 1st – 2012.
Inauguration of the ‘STAR COLLEGE SCHEME’ with
Guest Lectures
Dr. Raviraja N. S.
Centre Head Stemputics, Manipal.
“Stem Cells and their Applications”
National Level Seminar on ‘Emerging Trends in
Biological sciences’ on January 18- 19, 2013
Mr. Sammilan Shetty.
Butterflies & Their Conservation
Dr. Raviraja N. S.
Nanobiotechnology & its
Applications
Dr. Ethel Suman
Valedictory Address
Dr. Goldin Quadros
Role of Intertidal fauna along Mangrove
Ecosystem
Dr. George V. Thomas.
Keynote Address
Dr. Sheila Donde.
Brain Matters
Workshop on “Science in Action”– January, 8- 10, 2015 -
“Plastination”- students practicing process of plastination
Sheep lung plastinated
2012 - 2013
FACULTY IMPROVEMENT PROGRAMS INITIATED
FOR THE FACULTY
Three days training at Department of
Biotechnology , St. Aloysius College,
Mangaluru in Biological Techniques under
Tissue Culture
Gel Electrophoresis
Thin Layer Chromatography
No. of students : 05
Faculty : 02
Ten days ‘Hands on’ training at CPCRI
Kasargod.
1. Media types and preparation
2. Embryo culture - Coconut
3. Meristem culture - Palm oil
4. Leaf culture - Papaya.
5. Stem culture - Centella
6. Root culture - Carrot
7. Anther culture – Datura
No. of students : 08
Faculty: 02
2013 - 2014
Seminars Attended 2012 -13
Date Institution Title of the Programme
No. of students/
Faculty
April, 23-24, 2012
MGM College, Udupi
Principles and Techniques of Plant Taxonomy with a special reference to Western Ghats and Coastal Region of Karnataka
3+1
August,13 - 2012
Alvas College Moodbidri
Intercollegiate Competition Model on Cloning
Feb.8, 9. 2013
St. Aloysius college, Mangalore
National Seminar on Ferns
2 +1
March, 23, 2013
CPCRI, Kasargod
Training Cum Awarenes Programme “Protection of Plant Varieties and Farmers right act
4
Seminars Attended 2013 -14
Date
Institution
Title of the Programme
No. of students/
Faculty
July, 27. 2013 St. Aloysius College, Mangalore.
“Biospark: Catch them Young” 10 +1
Dec. 10, 11 2013
St. Aloysius College, Mangalore
“Trends in Biotechnology and its Appplications”
4 + 1
Dec.16,17 2013
St. Philomena College, Puttur
National Seminar “World Heritage Tag and conservation of Biodiversity”
4 +2
Jan. 23, 24. 2014
MGM College, Udupi.
National seminar on “Plant Biodiversity of Western Ghats and Sustainable management”
3+1 Paper presented
Feb. 03, 04 2014
SDM College, Ujire “Science Academies’ Lecture Workshop
4
Seminars Attended 2014 -15
Date
Institution
Title of the Programme
No. of
students
Paper
presented
Oct. 31. & Nov. 1, 2. 2014
Bharathidasan University, Tiruchirapalli
International conference on “Trends in Plant Systematics”
3 +1 Yes
Dec., 12. 2014
Vivekanada College, Puttur
Seminar on “Edible and non edible Mushrooms”
2+1 Yes II place
Dec., 17,18, 2014
University College, Mangaluru
“DNA Barcoding”- Concepts and Application
3+1 -
Feb., 6,7 –2015
Alva’s College, Moodbidri
“Advancements in Environmental Biotechnology for Sustainability
3+1 -
Feb., 14. 2015
Alva’s College, Moodbidri
University level Seminar presentation competition in Biological Sciences
2 I Place
Visit to Central Plantation Crop Research Institute, Kasargod
VISITS TO INDUSTRY AND IMPORTANT LABS OF NATIONAL
EMINENCE
CPCRI Genetic Resource and Plant Breeding
Tissue culture Lab Vermicomposting
Educational Tour organized from 14 – 17, November, 2013 to
Coimbatore and Ooty.
Visit to Tamilnadu
Agricultural University,
Departments of
Biotechnology,
Pathology, Plant
breeding and
Bioenergy.
Ooty Botanical garden
for plant diversity study
and collection of
Gymnosperms and Pteridophytes
Educational Tour from 14 – 17, November, 2014 to Wayanad, Mudumalai, Neelambore, Peechi.
1. M.S. Swaminathan Institute of biodiversity, Wayanad.
2. Kerala Forest Research Station Bioresource center, Neelambore.
3. Kerala Forest Research Station, Peechi.
Mudumalai Tiger Reserve.
Research Institutes visited
1. Plant collection at Madikeri on 21-01-2013 2. Fern Collection at Kudremukh on 10-02-2013 3. Algae collection at Bekal fort and Someshwar on 14-01-
2013 / 07-01-2014 4. Oddoor Farms – Yedapadav and Manipal on 15-03-2014 Production of biogas, Sustainable agricultural practice. Generation of electricity using biogas, solar lighting, Irrigation, rain water harvest etc.
Manipal: Visited Anatomy museum, Biological sciences
lab and planetorium.
Name Topic No. of students
Queenie Lasrado Alia orchids, M’lore.
“Orchids – Domestication and Conservation”
68
Dr. Sham Sunder JSS Medical College.
“Plastination - Importance in preservation”
52
LECTURES DELIVERED BY EXPERTS/SPEAKERS IN
RELEVANT SUBJECT AREA
“Basic Science Experimental Fair” for PUC students on
November 12, 2014
No. of students - 40
OUT REACH PROGRAMS
1. Identify the morphology of different plant groups. 2. Preparation of herbarium sheets 3. Study of distribution of stomata. 4. Separation of plant pigments. (Paper chromatography) 5. Germination of pollen grain on stigma. 6. Study of plant population by quadrat. 7. Extraction of DNA from coconut endosperm. 8. Preparation of synthetic seeds.
1. Estimation of Proteins
2. Estimation of carbohydrates 3. Extraction of DNA from coconut Endosperm 4. Preparation of synthetic seeds
Vacation School for Degree Students on December 1 – 4, 2014
No. of students - 28 from 6 Colleges
“Home Pharmacy” exhibition on medicinal plants and home remedies.
Display of Science/General articles on bulletin board
‘Plant of the week’ Display of one plant per week
with importance and key features
FERNS - Herbarium and Photograph Record
About 50 Herbarium sheets of Ferns are added to the Department Herbarium collected from Western
Ghats and A digital record is also maintained.
OUTCOMES OF THE SCHEME
Additional practicals in the curriculum enable students to have a better edge over affiliated college students.
Equipping the department with additional equipments and books
Opportunity to undertake project work thereby inculcating research culture among students.
Performing experiments individually rather than in group enhances confidence and accuracy.
Opportunity to attend seminars in different places. Paper presentations to enhance skills in practical work and presentation Exposure and interactive opportunities through guest lectures, seminars, training, field trips, industrial visits, Study tour.
ACTION PLAN
• Summer school: For affiliated college students.
• Workshop/Training: For Mangalore University Lecturers/
Lab assistants
• Industrial Visit/ Research Institute Visit:
• Student projects:
• Guest Lectures:
• Other if any:
Paper presentations on projects by the students.
Participation in Model competition.
Exhibition of medicinal plants.
Competition on seminar presentation.