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Last update: November 2018 STEVENIN Virginie (Ph.D.) 11/19/1990 Post-doctoral fellow at the Leiden University Medical Center EDUCATION 2018: PhD thesis in Microbiology at the Pasteur Institute and University Sorbone-Paris-Cité. 2014: Master degree's 2 nd year with Honors in Infectiology (Microbiology specialty). Pasteur Institute, Diderot University and École Normale Supérieure of Cachan (France). 2012: Master degree's 1 st year with Honors in Oncology. Paris Sud University and École Normale Supérieure of Cachan (France). 2011: Bachelor’s Degree in Biology. Paris Sud University and École Normale Supérieure of Cachan (France). 2010-2014: Admission into the Biology Department of the Ecole Normale Supérieure (ENS) of Cachan, an institute of higher education with highly selective admission requirements (20 students selected out of 800 applicants, each year). 09/2008-09/2010: CPGE in Lycée Michel Montaigne, Bordeaux (France). 2-year intensive undergraduate program preparing for the competitive entrance examinations to French top-level "Grandes Écoles". Major: Biology, Chemistry, Physics, and Mathematics. SCIENTIFIC EXPERIENCES 09/2014-09/2018: PhD thesis in the Unit “Dynamics of Host-Pathogen Interactions” under the direction of Jost Enninga at the Pasteur Institute, Paris. PhD project: Investigation of the differential intracellular localization of Salmonella enterica and its impact during the infection.” 12/2013-06/2014: 6 months internship in Infectiology in the Unit « Dynamics of Host-Pathogen Interactions » under the direction of Jost Enninga at the Pasteur Institute, Paris. Entitled: “Intracellular bacterial localization and host responses during bacterial infection: Impact of local context of the host cell during Salmonella infection.11/2012-08/2013: 10 months internship in Mechanobiology under the direction of Michael Sheetz at the Columbia University, New York. Entitled: “A role for formins in rigidity sensing of motile cells.” 06/2011-08/2011: 3 months internship in Parasitology under the direction of Isabelle Tardieux at the Cochin Institute, Paris (INSERM U1016 CNRS UMR 8104). Entitled: “Comparative analysis of fibroblasts permissivity for Toxoplasma gondii between WT host cells and cells overexpressing an activated form of Src kinase.11/2010: Short training period in the CHIPI unit headed by Dr. Alexandre Boissonnas (Inserm U1135), Pierre and Marie Curie University, Paris (France). Entitled: “Modulation of leucocyte recruitment during anti- tumoral chemotherapy.” TEACHING ACTIVITIES 2018: Supervision of a Master 1 student (Yoann Le Toquin) for 3 months internship. 2016: Supervision of a Master 1 student (Jakub Voznica) for 5 months internship. 09/2014-09/2017: Teaching responsibilities on cell biology. 64 h of teaching per years. Diderot University, Paris (France). virginie.stevenin@ens-cachan.fr +33 (0)6.85.49.01.64 Breestraat 19 2311CG Leiden The Netherlands
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Page 1: STEVENIN Virginie Ph D - Research...2011: Bachelor’s Degree in Biology. Paris Sud University and École Normale Supérieure of Cachan (France). 2010-2014: Admission into the Biology

Lastupdate:November2018

STEVENINVirginie(Ph.D.)11/19/1990Post-doctoralfellowattheLeidenUniversityMedicalCenterEDUCATION

2018:PhDthesisinMicrobiologyatthePasteurInstituteandUniversitySorbone-Paris-Cité.

2014:Master degree's 2nd year with Honors in Infectiology (Microbiology specialty). Pasteur Institute,DiderotUniversityandÉcoleNormaleSupérieureofCachan(France).

2012: Master degree's 1st year with Honors in Oncology. Paris Sud University and École NormaleSupérieureofCachan(France).

2011:Bachelor’sDegreeinBiology.ParisSudUniversityandÉcoleNormaleSupérieureofCachan(France).

2010-2014:Admission intotheBiologyDepartmentoftheEcoleNormaleSupérieure(ENS)ofCachan,aninstituteofhighereducationwithhighlyselectiveadmissionrequirements(20studentsselectedoutof800applicants,eachyear).

09/2008-09/2010: CPGE in Lycée Michel Montaigne, Bordeaux (France). 2-year intensive undergraduateprogrampreparingforthecompetitiveentranceexaminationstoFrenchtop-level"GrandesÉcoles".Major:Biology,Chemistry,Physics,andMathematics.

SCIENTIFICEXPERIENCES09/2014-09/2018:PhDthesis intheUnit“DynamicsofHost-PathogenInteractions”underthedirectionofJost Enninga at the Pasteur Institute, Paris. PhD project: “Investigation of the differential intracellularlocalizationofSalmonellaentericaanditsimpactduringtheinfection.”

12/2013-06/2014: 6 months internship in Infectiology in the Unit «Dynamics of Host-PathogenInteractions» under the direction of Jost Enninga at the Pasteur Institute, Paris. Entitled: “Intracellularbacterial localizationandhostresponsesduringbacterial infection: Impactof localcontextofthehostcellduringSalmonellainfection.”

11/2012-08/2013:10monthsinternship inMechanobiologyunderthedirectionofMichaelSheetzattheColumbiaUniversity,NewYork.Entitled:“Aroleforforminsinrigiditysensingofmotilecells.”

06/2011-08/2011: 3 months internship in Parasitology under the direction of Isabelle Tardieux at theCochin Institute, Paris (INSERM U1016 CNRS UMR 8104). Entitled: “Comparative analysis of fibroblastspermissivityforToxoplasmagondiibetweenWThostcellsandcellsoverexpressinganactivatedformofSrckinase.”

11/2010:ShorttrainingperiodintheCHIPIunitheadedbyDr.AlexandreBoissonnas(InsermU1135),Pierreand Marie Curie University, Paris (France). Entitled: “Modulation of leucocyte recruitment during anti-tumoralchemotherapy.”

TEACHINGACTIVITIES2018:SupervisionofaMaster1student(YoannLeToquin)for3monthsinternship.

2016:SupervisionofaMaster1student(JakubVoznica)for5monthsinternship.

09/2014-09/2017:Teaching responsibilitiesoncellbiology.64hof teachingperyears.DiderotUniversity,Paris(France).

[email protected]+33(0)6.85.49.01.64

Breestraat192311CGLeiden

TheNetherlands

Page 2: STEVENIN Virginie Ph D - Research...2011: Bachelor’s Degree in Biology. Paris Sud University and École Normale Supérieure of Cachan (France). 2010-2014: Admission into the Biology

Lastupdate:November2018

PUBLICATIONSHigh-throughputmicroscopicanalysisofSalmonellainvasionofhostcells.VoznicaJ,EnningaJ,StéveninV*-(*)correspondingauthor.BioProtoc.2018.doi:10.21769/BioProtoc.3017.

TheearlyentryofSalmonellainadistincttightcompartmentrevealedathightemporalandultrastructuralresolution. Fredlund J (#), Santos JC (#), Stévenin V (#), Weiner A, Rechav K,Mallet A, Krijnse-Locker J,ElbaumM,EnningaJ–(#)equalcontributors;CellMicrobiol,2017.doi:10.1111/cmi.12816

IdentifyingparametersofhostcellvulnerabilityduringSalmonellainfectionbyquantitativeimageanalysisandmodeling.VoznicaJ,GardellaC,BelotserkovskyI,DufourA,EnningaJ,StéveninV*-(*)correspondingauthor.InfectImmun,2017.doi:10.1128/IAI.00644-17.

FHODisneededfordirectedforcesandadhesionmaturationduringcellspreadingandmigration.IskratschT, Yu CH, Mathur A, Liu S, Stévenin V, Dwyer J, Hone J, Ehler E, Sheetz M. Dev Cell, 2013. doi:10.1016/j.devcel.2013.11.003.

Manuscriptinpreparation/submitted:

SizeregulationoftheSalmonella-containingvacuoledeterminesSalmonellaintracellularlifestyleinepithelialcells.StéveninV,ChangYY,LeToquinY,DuchateauM,GiaiGianettoQ,SohstV,ReilingN,MatonboM,EnningaJ.Researcharticle.inpreparation.

Cellularimagingofintracellularbacterialpathogens.StéveninVandEnningaJ.BookChapter.underrevision

FUNDINGS(Grantsandfellowships)05/2018-06/2018:2monthsPhDextensionfellowshipprovidedbythedepartmentofCellBiologyandInfectionofthePasteurInstitute(Paris,France).

10/2017-05/2018:7monthsPhDextensionfellowshipprovidedbythe“FoundationforMedicalResearch”(FRM).

09/2017:TravelgrantfortheEMBOConference:“Endocytictraffickingandsignalinginhealthanddisease”providedbytheFrenchSocietyforCellBiology(SBCF).

05/2017: Travel grant for the EMBO|EMBL symposium: “Molecular and Cell Biology of Membranes”providedby“GlobalCareInitiative-PasteurMicrobeetSanté”.

09/2014-09/2017:3years"Normalien"-specificPhDfellowship fromtheFrenchMinisterofResearchandEcoleNormaleSuperieure(ENS)deCachan(France).

09/2012-08/2014: "Normalien" status (highly selective French civil servant status for foundedundergraduatestudies)attributedbytheFrenchMinisterofResearchandtheÉcoleNormaleSupérieureofCachan(France).

09/2008-09/2012:FrenchMinistryofEducationmeritscholarship.

HONORS/PRIZES

03/2018:CoverimageoftheApril(2018)issueofCellularMicrobiology.

02/2018:Selectionofmyco-first-authorarticle,as“Editors’Choice”inCellularMicrobiology.

11/2017:Selectionofmy last-authorarticlebytheeditorsof Infectionand Immunity tobehighlighted in

the“Spotlight”sectionofthejournal.

09/2016: Award of the best poster during the 5th congress on Salmonella of the American Society forMicrobiology,Potsdam.

Page 3: STEVENIN Virginie Ph D - Research...2011: Bachelor’s Degree in Biology. Paris Sud University and École Normale Supérieure of Cachan (France). 2010-2014: Admission into the Biology

Lastupdate:November2018

SCIENTIFICPRESENTATIONSTalks:

05/2018: Invited atOsloUniversity Hospital, Institute for Cancer Research (Norway). Talk entitled: “NewinsightsonSalmonellaintracellularlifestyleinepithelialcells”

02/2018:1stMeetingonVesicularBiology(Coïmbra,Portugal).Shorttalkentitled:“ThevacuolarlifestyleofSalmonella in epithelial cells is determined at the early stage by the communication between distinctinfectionassociatedcompartments”

01/2018:InvitedattheLeidenUniversityMedicalCenter(TheNetherlands).Talkentitled:“NewinsightsonSalmonellaintracellularlifestyleinepithelialcells”

10/2017:2ndmeetingofthelaureatesoftheSchlumbergerFoundationforEducationandResearch(FSER),and their teams (Imagine Institute, Paris, France). Short talk entitled: “Communication between distinctinfectionassociatedcompartmentsdrivestheintracellularlifestyleofSalmonella”.

09/2017:EMBOConference“Endocytictraffickingandsignalinginhealthanddisease”.Shorttalkentitled:“Communication between distinct infection associated compartments drives the intracellular lifestyle ofSalmonella”.

03/2017:“Work-in-Progress”presentationfortheCellBiologyandInfectiondepartment(PasteurInstitute,Paris,France).Talkentitled:“QuantifyingthevulnerabilityofsinglehostcellstoSalmonellainfection”

11/2016:DepartmentaldayoftheCellBiologyandInfectiondepartment(PasteurInstitute,Paris,France).Short talk entitled: “The interactions between Infection Associated Macropinosomes and enteringSalmonelladetermineitsintracellularniche.”

05/2016:Conference“YoungResearchersinLifeSciences2016”(Paris,France).Shorttalkentitled:“Howtofeellikehome:DissectingthefirststepsofSalmonellaintracellularlife.”

Posters:10/2018:EMBOConference“Endoplasmicreticulumfunctioninhealthanddisease”(Lucca,Italy).

10/2017: Departmental retreat of the Cell Biology and Infection department of the Pasteur Institute(Pornichet,France).

09/2017:EMBOConference“Endocytictraffickingandsignalinginhealthanddisease”.

05/2017: EMBO|EMBL Conference “Molecular and Cell Biology of Membranes” (EMBL, Heidelberg,Germany).

10/2016:PasteurMeeting“QuantitativeBiologyKick-offMeeting”(PasteurInstitute,Paris,France).

09/2016:5thSalmonellaConferenceoftheAmericanSocietyforMicrobiology(Potsdam,Germany).

06/2015: Summer school FEBS/EMBO“Biomembranes: Molecular Architecture, Dynamics and Function”(Cargèse,France).

05/2015:Conference“YoungResearchersinLifeSciences2015”(Paris,France).

12/2012:Conference“AmericanSocietyforCellBiology”(SanFrancisco,USA).

SCIENTIFICEVENTSORGANIZED2016-2017: Organization of the scientific conference “Young Researchers in Life Sciences2017” (ImagineInstitute,Paris,France).

2015-2017: Coordinator for the association Animath proposing mathematical activities for high-schoolstudents.


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