A iosti ula t app oa hto edu i g st ess o you tu f
a d i p o i g tu f uality - ith i depe de t t ial data
Product Foliar Rate/Ha Active Benefits Cost/Ha
Phylgreen 15 Ascophyllum nodosum Improve root growth/heat tolerance 290
Alexin 5 Salicylic acid Improve ability of plant to fight disease 76
Delfan 2 24% amino acids; 9% N; 23% carbon Improve plant recovery 35
EDTA Ca 5 14% w/w calcium oxide (CaO) Harden cell wall 148
Silica 1-2 100% silica Harden turf 80-160
Green Fer Energy 5-8 21% iron EDDHA; 17% Humic acid Improve colour and root growth 80-161Monthly
Frequency
Monthly
Monthly
Monthly
Monthly
Monthly
Why is this approach beneficial ?
STRESS Ma age e t
Suppl of o ple sugars Suppl of a tio ida ts
Suppl i oligo-ele e ts
Suppl of Potassiu
Suppl i os oregula ts
MIX Ph lg ee WITH YOUR OTHER FOLIAR PRODUCT“ !
Appli atio : s all doses ut egula
CONTACT EFFECT
pH EFFECT
SYNERGIES ith Catio s Fe, Ca, Mg, M …
Effe ts: MAIN BENEFITS.
REDUCE stress effe ts fro soil a d eather o ditio s
E ha e faster re o er after frost, draught or sali it stress
I pro e utrie t a sorptio espe iall ke ele e ts Mai l atio s like K+, Ca +, Mg +, Fe +, Se+…
I rease i ro iologi al di ersit of the soils
Pseudomonas sp., Bacillus subtilis�
I pro e the root de elop e t i parti ular root le gth
Redu e da agi g effe ts of pathoge s a d pesti ide
treat e ts effe t a idi pH a d a tio ida ts
L A N D L A B
Drought Stress
La dla Resea h “tatio Qui to Vi e to Ital D . Ad ia o Altissi o Rossella Bo tolaso
T ials ea
- “oil: % sa d + % la dla
- epli ates / plot . – “tats ANOVA
- Lolium perenne & Festuca rubra
- 0 % (high stress), 25% (medium Stress) and 50% (low stress)
of Evapotranspiration (water restitution using sprinkler)
Drought Stress E peri e tal Setup:
Sprinkler for irrigation
- Spraying of 0 (control), 30 and 60 litres AG200 every 3 weeks
Para eters Assessed:
- % Li i g G ou d Co e LGC usi g Digital I age A al sis / eek
- Visual Tu f Qualit TQ afte ea h ut - Chlo oph ll o te t efo e e e ut
Chlorophyll Meter (CM) - Che k of the soil hu idit / eek
Drought Stress d Mai Out o e: R eg ass Lolium perenne – Be efits of phylgree
Co t ol L/Ha
-As the stress i rease, the TQ is de reasi g
.
.
.
.T
Q (
Tu
rf Q
ua
lity
)
Ryegrass -Turf Quality at high drought stress
Drought Stress d Mai Out o e: R eg ass Lolium perenne – Be efits of the phylgree
Co t ol L/Ha
As the st ess i ease, the Chlo oph ll o te t is de easi g
. . . . . . . . . . . . . . . . . .
CM
Ryegrass - Chlorophyll Measure (CM) at high drought stress
Drought Stress rd Mai Out o e: R eg ass Lolium perenne – Be efits of the phylgree
Co t ol L/Ha .
.
.
.
.
.
.
.
.
-“ep -“ep -O t -O t -O t -O t -No -No
% Dr Pat hes o R egrass after phylgree treat e t i high drought stress
Co t ol
15 L/ha
Drought Stress
Co trol 15 L/Ha R eg ass Lolium perenne – % ate estitutio ediu st ess – e d of t ial De e e
.9 .
. .
. .
% Gree
Chlo. M.
% D
Meloidogyne minor
Nematodes
“ pto s o a g ee …
Pi tu e ou tes of D . Coli Fle i g
RESULTS RELATION TO NEMATODES i e peri e ts ith AFBI,
I ease of Ne atode ates i o t ol a d significant de ease i Ne ati ide Mai Co lusio s:
AG pu e a d AG Roots a e li iti g de elop e t of e atodes at ost of appli atio s Effe t of o petitio et ee the i o ial life a d Ne atodes
Nematodes
Start of Autu Start of Spri g
Application:
Small doses but frequent
“t ess e ha i al
“t ess heat / ate
Possi le “t ess Fu gi
“t ess F ost
Possi le “t ess A so ptio
N, P
Oligo elts
K AG Hu i s AG Pu e
Use as ase produ t for a foliar spra i g – i ith other produ ts i parti ular fu gi ides
A atu al i of Biosti ula tsBiostimulant
Type/Familly
Active
compounds
Main
Origin
Action
Plant
Growth
Regulators
(PGR)
Plant hormones
Amino Acids
Humic acids
Sugars (Carbon)
Bacteria
Synthetic
Synthetic
Natural
Natural
Natural
Enhance development
of key phases:
Roots, leaf, flowers…
(direct or indirect)
Plant Defense
Stimulators
Auxines
Glutamic acid
Oligosaccharides
Synthetic
Both
Natural
Direct stimulation of
Plant defense system
Osmoregulants
Antioxidants
Glycin Betain
Mannitol
Salicylic acid
Vitamins, tannins
Natural
Natural
Both
Both
Help plant regulate
Water inside/outside
Neutralise free radicals
Biofungicides
Biopesticides
Trichoderma sp.
Garlic, Mustard…
Natural
Natural
Compete with pathos
Act on pest physiology
Seaweed
Cold Extracts -
Plant
Hormone like (+) ,
A.A. (+),
Sugars (+++)
Auxins (+),
Glutamic Acid (+)
Oligosaccharides (+++)
Mannitol (++),
Vitamins (+,
Tannins (++)
In addition:
Alginates (- charged) =
surfactant & water retention
Free amino acids – content of amino acids that can be used fast by the plant
Total amino acids – content of free and peptide amino acids. Only part of the total amino acids can be used
Peptides - short amino acid strains with two or more amino acids
Proteins - long chains of amino acids folded in a special way. Proteins have important functions in plant metabolism
Definitions
Delfan Plus is extremely high concentrated in free amino acids, 24%. Products based on enzymatic hydrolysis
have a lower content of free amino acids
Delfan Plus – technical specifications
PHYSICAL-CHEMICAL CHARACTERISTICS Aspect: Liquid Colour: Brown Density: 1,2 g/cc
pH: 7,2 CHEMICAL ANALYSIS
Parameter Guaranteed value (% w/w) Admitted deviation* Free aminoacids** 24,00% -1,20
Total nitrogen (N) 9,00% -0,90
Organic matter 37,00% -3,00 Organic carbon 23,00% -2,00
The importance of the aminogram
Delfan Plus aminogram
Histidine
Threonine
Methionine
Tryptophane
Valine
Arginine
Serine
Lysine
Phenylalanine
Isoleucine
Leucine
Aspartic acid
Tyrosine
Hydroxyproline
Proline
Alanine
Glycine
Glutamic acid
0
2
4
6
8
10
12
14
free
AA c
onte
nt [%
w/w
]
Vegetable aminogram vs. Delfan Plus
Typical vegetal AA from soybean and wheat Delfan Plus
No Hydroxiproline and little proline
Differences in aminograms
The importance of Proline and Hydroxiproline
Proline is an excellent anti stress agent and leads to tolerance to a wide range of abiotic stresses
Proline is especially important during osmotic stress (drought, salinity and freezing)
Proline leads to:
Osmotic adjustment (Compatible osmolyte)
Stabilized cellular structures
Prevention of cell damage due to free radicals (Radical Scavenger)
Provides energy needed for recovery
Protein synthesis
DNA synthesis
Precursors of hormones
Precursors of other functional molecules
Stress metabolism
Functions of amino acids in plants
Amino acids are the building blocks of life
Without amino acids there is no life
Functions of amino acids
Amino acid Effect or function All AA Protein synthesis Glycine DNA synthesis, Alcaloid metabolism Glutamic acid Chlorophyll synthesis Tryptophane Auxin and phytoalexin precursor Methionine Ethylen and polyamine precursor Aspartate, glutamine and glutamate
N and C storage amino acids, transport amino acids
Proline stress metabolism, flowering Serine Precursor glycine betaine, stress metabolism
Alanine Precursor of certain antibiotics in some species
Leucine, Lysine, tryptophane, histidine, phenylalanine, tyrosine and glycine
Alcaloid metabolism, plant protection against pests and stress
Phenylalanine Salicylic acid production, stress and disease prevention
Tyrosine Glucosinolate precursors “Phytoanticipins”
Methionine
Arginine
Tryptophane
Rest
Delfan Plus content of hormone precursor amino acids
Serine
Proline
Rest
Osmolyte content of Delfan Plus
34.5
35
35.5
36
36.5
37
37.5
Control Delfan
Yiel
d [T
/ha]
Yield of potatoes after Delfan applications of 1.5L/ha compared to standard in a dry year
Delfan effect on potatoes during dry conditions
Delfan and stress prevention
Delfan contains osmoprotectants proline, hydroxiproline and serine
Better tolerance to saline conditions
Protection against dry spells
Enhanced cold hardiness
Enhanced yield
Introduction
Need for more proof Years of independent trial data Consistent, even application of nutrient across turf surface Faster establishment of new turf Enhanced turf quality Improved soil and plant fertility Less leaching of nutrient from the root zone Saving to fertiliser budgets often achievable Simple, integrated plan Easy to use, clean products – boom or fertigation applied
90
Years of R&D done in Broad Acre Ag
Trial Data Unique to Microsoil (broad acre ag) & a focus of Microturf Most trial work has been done via boom application Independent proof of products’ ability to enhance plant nutrition Identified best practice with liquids With or without granular fertiliser
Ongoing trial work is integral to ongoing product development Highlighted inefficiencies of granular fertiliser
LIQUID INJECT TRIAL
97
Treatment Yield T/ha Protein % Net Gain/ha on MAP
SFP =80kg MAP 3.02 8.7% 0
MICROSOIL CROP STARTER + 35kg MAP + AWAKEN ST 3.60 10.3% $179.15
AWAKEN ST + 80kg MAP 3.11 10.3% $20.20
AWAKEN ST + 80kg MAP + AWAKEN 3.37 10.3% $81
AWAKEN ST + 80kg MAP + NUTRISYNC M 3.16 8.1% $23.25
AWAKEN ST +80kg MAP + MAXIMUM N PACT 3.15 10.2% -$1.60
WHEAT price = $280/T
2010-2012 Turf Trial (Buffalo) Windsor NSW
Details: This long term trial was conducted over some difficult growing seasons in Sydney. The Turf business set key indicators for trial success as being: improved turf and soil quality; greater speed to market; reduced fertiliser costs/more efficient use of applied nutrients; a more sustainable nutrition plan with less emissions and increased soil carbon and finally faster establishment of the following turf. A one HA block was used for the trial, half of which continued to have applied an industry standard turf program of synthetic fertiliser costing approx $1,500/HA. 25% of the block received half that amount of standard synthetic fertiliser ($750/HA) along with Microturf’s range of liquid biological mineral fertilisers. The remaining 25% received no synthetic fertiliser and just the Microturf liquids. Comments from the Turf Manager were that throughout the seasons the areas ‘Treated’ with Microturf were visibly ahead in their development compared to the standard ‘Untreated’ areas of the block. This included the establishment and appearance of the turf and importantly how the turf cut. The subsequent turf receiving the same treatments continued to show marked improvements in the Microturf ‘Treated’ areas. A soil testing program was conducted throughout the trial and the results tell a very compelling story about nutrient efficiency and the ability to eliminate wastage from intensive fertiliser programs such as those commonly adopted in Turf and Horticulture programs. A snapshot of the soil tests can be seen here. Note that despite significantly less units of fertiliser being applied in the 'Treated' areas where Microturf's liquid fertilisers were used, these areas continually showed up higher levels of available nutrient when compared to the ‘Untreated’ areas that received synthetic fertiliser at $1500/HA . Organic matter and soil Carbon levels also increased in Microturf ‘Treated’ areas.
Sir Walter Trial Layout 2010-2012 Windsor NSW
U
1 HA trial block on Wilberforce Rd Windsor.
Sta
nd
ard
Sy
nth
eti
c F
ert
Pro
gra
m
½ S
tan
dard
Fert
Pro
gra
m +
Mic
rotu
rf
Mic
rotu
rf o
nly
Wilberforce Rd
Drive W
ay
STD kg/ha
N 264
P 78
K 210
S 102
Ca 38
Mg 18
101
Key Indicators
Summer Grass Dormancy Trial ACT (Buffalo) – 28th Sept 2012 Details: This trial was conducted over a 12 week period in Canberra where summer grasses often remain in a dormant state until the end of December. The trial aimed to gauge the success of a fast recovery from dormancy in conjunction with monitoring any improvements in turf conditions over a three month period to ensure that any gains were not short lived. The Microturf trial products were applied to buffalo grass on the 28th September 2012. The entire field and the ‘Control’ had received a standard application of granular synthetic fertiliser prior. The Microturf products applied were: PRO NITROGEN; PROMOTE, PRO CAL+ and PRONTO. The trial was monitored over 9 occasions during the 12 week period where a visual record of the turf colour, density and health was noted. A tissue sample was taken from each of the plots including the ‘Control’ on the 8th November 2012. Plots with Microturf liquid fertiliser applied consistently showed up improved nutrient levels over the Control in key areas testifying to Microturf’s ability to quickly enhance plant nutrition levels. Comments from the Turf Manager were that from the very first week of the trial onwards the plots treated with Microturf products were significantly ahead of the control in terms of colour and plant vigour. PRONTO was the initial standout performer with PROMOTE & PROCAL+ showing improvement from week two followed by PRO NITROGEN in week three. PROMOTE showed the best longevity throughout the 12 week trial with all plots coming back to an even state as conditions warmed up at the 12 week period.
PHOTOS TAKEN 1ST November 2012 Summer Grass Dormancy Trial ACT – 28th Sept
PLOT 5 PLOT 8
PLOT 4 PLOT 1
PLANT TISSUE REPORT - BUFFALO DORMANCY RECOVERY TRIAL
SITE: Canberra
Teatment applied 28th September 2012
Plot 1 Plot 4 Plot 5 Plot 8 Control Tissue Test Date 8/11/2012 8/11/2012 8/11/2012 8/11/2012 8/11/2012 MICROTURF PRODUCT
Plant Tissue Analysis Nitrogen 2.22 1.69 2.64 1.63 2.01 Phosphorus 0.39 0.32 0.36 0.34 0.29 Potassium 1.5 1.3 1.4 1.5 1.2 Sulphur 0.30 0.27 0.33 0.29 0.31 Calcium 0.40 0.29 0.44 0.28 0.41 Magnesium 0.24 0.19 0.25 0.2 0.21 Sodium 0.75 0.68 1.22 0.57 0.76 Iron 1782 1446 1407 1693 1127 Manganese 73 51 60 88 51 Zinc 90 48 57 124 56 Copper 14 10 13 12 10 Boron 22 21 20.1 18.8 16.2 Molybdenum 0.10 0.10 0.1 0.1 0.1
2012 Ribbon Trial (Buffalo) – Canberra ACT
Details: This trial was conducted over a 12 week period in Canberra where summer grasses often remain in a dormant state until the end of December. The trial aimed to gauge the success of a fast recovery from dormancy in conjunction with monitoring any improvements in turf conditions over a three month period to ensure that any gains were not short lived. The Microturf trial products were applied to buffalo runners on the 1st November 2012. The entire field and the ‘Control’ had received a standard application of granular synthetic fertiliser prior. The Microturf products were applied to four plots which were pegged out for trial measurement. The trial was monitored over a 10 week period where a visual record of the row closure was noted along with turf colour, density and health. Comments from the Turf Manager were that the plots treated with Microturf products were ahead of the control in terms of their development. Runners were staked out and in the treated areas they were shown to be more frequent and they grew ‘across the deck’ faster with less of a ‘dolphining’ effect. The demonstration also showed that areas treated with Microturf products had better colour in ribbons with greater biomass.
PRODUCTS APPLIED 1ST November 2012 Ribbon Trial (Buffalo) ACT
PLOT 5 PLOT 8
1ST NOV 19TH NOV
19TH NOV 19TH NOV
Sand Based Sports Fields Limited media for plant & root development Plus lot of irrigation Less leaching Pro Nitrogen Very happy with results Golf – greens stayed green longer Football – better root dev & turf recovery. Helped with
Rhizoctonia & Pythium issues & new emergence
A liquid organic nutrient complex containing
salicylate derivates to boost the immune response of the plant. Alexin can safely be included in a disease
management programme and is highly tank compatible.
Salycilic acid Plants manufacture salicylic acid to trigger natural defences against disease.
The addition of Salycilic acid acts as
an activator of ‘Systemic Acquired Resistance’
(SAR). However, plants often don’t produce
the acid quickly enough to prevent injury when
attacked by a pathogen and so spraying
Alexin enables the ability of the plant to fight against infection.
Plants have never been totally defenceless
against disease attack. The issue has been
that often plants do not recognize their microbial
attackers in time.
Spraying salicylic acid in the form of Alexin
puts their defences on high-alert against future
attacks.
Research has found:
• Salt stress (salty water) (Borsani et al., 2001). improved plant tolerance to heat on creeping bentgrass (Larkindale and Huang, 2004)
• Improved heat tolerance of Kentucky bluegrass in a