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Supplemental figure A FA-D2 FA-D2 +FANCD2 FA-D2 +FANCD2(K561R) No HU 0.1mM HU S phase: 43% S phase: 34.7% S phase: 30.8% S phase: 52.7% S phase: 25.4% S phase: 21.9%
| Date post: | 17-Dec-2015 |
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Supplemental figure A
FA-D2 FA-D2+FANCD2
FA-D2+FANCD2(K561R)
No HU
0.1mM HU
S phase: 43% S phase: 34.7% S phase: 30.8%
S phase: 52.7% S phase: 25.4% S phase: 21.9%
Supplemental figure B
Input IgG FlagAb
IP
Rad18
Rad51
FACND2
Blot:
1 2 3
Supplemental figure C
- HU - HU
Whole cell
Blot:C Rad51siRNA
- HU - HU
Chromatin
Blot:C Rad51siRNA
Rad51
Ku86
USP1
Rad51
Ku86
USP1
1 2 3 4 1 2 3 4
Supplemental figure D
Blot:- MMC HU - MMC HU - MMC HU
Chromatin
Rad51-7 Rad51-2siRNA CBlot:
- MMC HU - MMC HU - MMC HU
Chromatin
FANCD2-18 FANCD2-2siRNA C
PCNA
Ku86
Ub-PCNA
Ku86
PCNA
Ub-PCNA
FANCD2 FANCD2
Blot:- MMC HU - MMC HU - MMC HU
Whole cell
Rad51-7 Rad51-2siRNA CBlot:
- MMC HU - MMC HU - MMC HU
Whole cell
FANCD2-18 FANCD2-2siRNA C
Rad51
Ku86
FANCD2
PCNA
Rad51
Ku86
FANCD2
PCNA
Supplemental figure D (continued)
Blot:- MMC HU - MMC HU - MMC HU - MMC HU - MMC HU
Soluble
FANCD2-18 FANCD2-2siRNA C Rad51-7 Rad51-2
PCNA
Ku86
FANCD2
PCNALong exposure
Supplemental figure E
PCNA
Ku86
- HU - HU
DMSO B02
DMSO B02Whole cell
Chromatin
Blot:- HU - HU
PCNA
Ku86
Blot:Ub-PCNA
Supplemental figure F
PCNA
Ku86
- HU - HU
BRCA2-/- BRCA2+/+BRCA2-/- BRCA2+/+
Whole cellChromatin
Blot:- HU - HU
PCNA
Ku86
Blot:Ub-PCNA
Supplemental figure G
Blot:- MMC HU - MMC HU - MMC HU - MMC HU - MMC HU
Chromatin
FANCD2-18 FANCD2-2siRNA C Rad51-7 Rad51-2
Blot:- MMC HU - MMC HU - MMC HU - MMC HU - MMC HU
Whole cell
FANCD2-18 FANCD2-2siRNA C Rad51-7 Rad51-2
PolH
Ku86
FANCD2
Rad51
Ku86
FANCD2
PolH
Supplemental figure H
Ku86
Blot:
Rad51
siRNA C 7 2
Rad51
