Synthetic Biology Crash CourseDAY ONE

Pre-Assessment1:00-1:10

Who We Are

Course ScheduleDay One

-Overview of Key Biology Concepts-Overview of Lab Protocols

Day Two-Lab Activities: Creating a BioBrick

Day Three-Modelling Workshop-Ethics Workshop

Day One Schedule

1:00-1:15 - Introduction and Pre-Assessment1:15-1:45 - General Biology Refresher1:45-2:15 - Introduction to Synthetic Biology 2:15-2:30 - Micropipetting Exercise

What Is Synthetic Biology?“...The design and construction of new biological entities such as enzymes, genetic circuits, and cells or the redesign of existing biological systems.”

Synberc (Synthetic Biology Engineering Research Center)

DNA and Genes: The Basis of Synthetic Biology1:15 – 1:45

DNA and RNA

DNA at Work

Replication

Transcription

Translation

Genes

Gene Constructs

Plasmids

BioBrick Formation1:45-2:15

Isolating a GenePolymerase Chain Reaction (PCR)

Restriction Enzyme Digest

Gel Electrophoresis

Ligation Reaction

Transformation Reaction

Confirmation

Activity: Micropipetting2:15-2:30

Homework● Read the following selections from the

“vGEM Protocol Handbook”○ Restriction Enzyme Digest○ Gel Electrophoresis ○ Transformation and Plating

● Explore the iGEM Parts Registry: http://parts.igem.org/Main_Page