-Overview of Key Biology Concepts-Overview of Lab Protocols
Day Two-Lab Activities: Creating a BioBrick
Day Three-Modelling Workshop-Ethics Workshop
Day One Schedule
1:00-1:15 - Introduction and Pre-Assessment1:15-1:45 - General Biology Refresher1:45-2:15 - Introduction to Synthetic Biology 2:15-2:30 - Micropipetting Exercise
What Is Synthetic Biology?“...The design and construction of new biological entities such as enzymes, genetic circuits, and cells or the redesign of existing biological systems.”
Synberc (Synthetic Biology Engineering Research Center)
DNA and Genes: The Basis of Synthetic Biology1:15 – 1:45
DNA and RNA
DNA at Work
Replication
Transcription
Translation
Genes
Gene Constructs
Plasmids
BioBrick Formation1:45-2:15
Isolating a GenePolymerase Chain Reaction (PCR)
Restriction Enzyme Digest
Gel Electrophoresis
Ligation Reaction
Transformation Reaction
Confirmation
Activity: Micropipetting2:15-2:30
Homework● Read the following selections from the
“vGEM Protocol Handbook”○ Restriction Enzyme Digest○ Gel Electrophoresis ○ Transformation and Plating
● Explore the iGEM Parts Registry: http://parts.igem.org/Main_Page