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Thermo Scientific TMT10plex Reagents
Applications:
• Protein identification and quantitation from multiple samples of cells, tissue or biological fluids
• Protein expression profiling of normal vs. disease states or control vs. treated
• Multiplex up to 10 different samples concurrently in a single experiment
• Quantitative analysis of proteins for which no antibodies are available
• Identification and quantitation of membrane and post-translationally modified proteins
• Identification and quantification of hundreds to thousands of proteins in a single experiment
References1. Thompson, A., et al. (2003). Tandem mass tags: a
novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS. Anal. Chem. 75(8):1895-1904.
2. McAllister, G.C., et al. (2012). Increasing the multiplexing capacity of TMTs using reporter ion isotopologues with isobaric masses. Anal. Chem. 84(17):7469-78.
3. Wenger, C.D., et al. (2011). Gas-phase purification enables accurate, multiplexed proteome quantification with isobaric tagging. Nat. Methods 8(11):933-5.
Visit thermoscientific.com/protein-custom or contact our Large Volume and Custom Sales Team directly at +815-968-0747 ext. 300, at 800 874 3723 ext. 300 or [email protected]
informationfor more product
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Figure 2. Procedure summary for MS experiments with Thermo Scientific TMT10plex Isobaric Mass Tagging Reagents. Protein extracts isolated from cells or tissues are reduced, alkylated and digested overnight. Samples are labeled with the TMT Reagents and then mixed before sample fractionation and clean up. Labeled samples are analyzed by high-resolution Orbitrap LC-MS/MS before data analysis to identify peptides and quantify reporter ion relative abundance.
Figure 3. Example of 10-plex relative quantitation using Thermo Scientific TMT10plex Reagents. BSA tryptic digests labeled with TMT10plex reagents (TMT10 126-131Da ) were mixed 16:8:4:2:1:1:2:4:8:16 and analyzed by high-resolution (Oribitrap LC-MS). The relative abundance of the target protein or peptide fragment in 10 different samples is easily measured by comparing the reporter ions generated by MS/MS fragmentation of the different mass tags.
TMT10-126
TMT10-127N
TMT10-128N
TMT10-129N
TMT10-130N
TMT10-131
Label
TMT10-127C
TMT10-128C
TMT10-129C
TMT10-130C
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TreatSamples& IsolateProteins
Denature,Reduce,
Alkylate &TrypticDigest
Fractionate,Clean-up,Perform
LC-MS/MSanalysis
Combine
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MS Selection MS/MS Quantitation
Life Science Research
Africa/Belgium/Europe/Middle East +32 53 85 71 84France 0 800 50 82 15Germany 0228 9125650Netherlands 076 50 31 880Switzerland 0800 56 31 40 UK 0800 252 185
Email: [email protected] thermoscientific.com/pierce
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1602641 04/13 Printed in the U.S.
thermoscientific.com/pierce© 2013 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only. Tandem Mass Tag, TMT10plex, TMTduplex, TMTsixplex, TMTzero and TMT are trademarks of Proteome Sciences plc. Facebook is a trademark of Facebook, Inc. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are available in all countries. Please consult your local sales representative for details.
