TIPS FOR ELEMENTAL ANALYSIS OF BIOLOGICAL SAMPLES

Lead in blood and urine Pica in children Urban poisoning OSHA regulations

Hg, As, and Cd Insecticides Certain fish Water and air Toys

Forensic applications Intentional poisoning Weapon ID

Essential trace nutrients Se as antioxidant Cr for glucose metabolism Co for Vitamin B12

CLINICAL APPLICATIONS OF ICP

CHALLENGES OF CLINICAL SAMPLES

Challenges:

Homogeneity Complex matrix Limited volume Speciation

Solutions:

Digestion; sonication; sample sizeNeb, torch, injectorMicro nebs, increased

transportHPLC-ICP-MS

CHOOSING THE RIGHT NEBULIZER

ApplicationHigh dissolved solids

Particulates and high TDS

Limited volume

Very small sample

Nebulizer SeaSpray

VeeSpray

Low uptake SeaSpray (0.4ml/min)MicroMist

(0.05ml/min)

TORCH SELECTION

Injector shape Aerosol dilution Outer tube

material

PATENTED D-TORCH

Demountable outer tube

Demountable injector

Optional ceramic outer tube

Economical price

QUARTZ VS. CERAMIC

6 HOURS OF RUNNING 10% NaCl

Quartz outer tube Ceramic outer tube

WHY SPECIATION?

Bad form AsIII, AsV CrVI (CH3)Hg BrO3-

Not so badMMA, DMA CrIIIHgII Br-

Increasing requirement of speciation methods for toxicity limits (As, S, Cr).

Need for reliable HPLC and IC interface to your ICP.

HPLC & IC INTERFACE KIT

• Resists blockage• Fast washout

• Zero dead volume• Minimizes peak broadening

The Answer: Glass Expansion LC Fittings Kit Quick and easy connection of your HPLC & IC to your ICP.

Compatible with any GE U-Series nebulizer.

U-SERIES NEBULIZER(SeaSpray)

Made from constant bore thick walled tubing

Machined exterior provides proper aerodynamics

Heated and drawn glass capillary

Variation in wall thickness

Variation in bore ID

ANALYSIS OF LIMITED SAMPLE VOLUMES

Biological applications Intracellular fluids Neonatal samples

Forensic applicationsWaste reduction applications Radioactive samples Toxic samples

Requires micro-flow nebulization Sensitivity and DL’s usually sacrificed

EFFECT OF TEMPERATURE ON SENSITIVITY

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

Al 394.4

01

Cd 214.44

0

Cd 226.50

2

Co 238.89

2

Cr 267

.716

Cr 205

.560

Cu 327.39

3

Cu 324.75

2

Fe 238

.204

Ni 231

.604

Ni 221

.648

Pb 220

.353

Se 196

.026

V 292.46

4

Zn 206

.200

Zn 213

.857

Zn 202

.548

Rela

tive

Sens

itivi

ty

40C60C21C

PE Optima 2100DV

(20μL/min uptake)

EFFECT OF TEMPERATURE ON DL

PE Optima 2100DV

0

2

4

6

8

10

12

Al 396

.153

Al 394

.401

Be 313

.107

Cd 214

.440

Cd 226

.502

Cr 267

.716

Cr 205

.560

Cu 327

.393

Cu 324

.752

Fe 238

.204

Mn 257

.610

Ni 231

.604

Ni 221

.648

Pb 220

.353

Se 196

.026

V 292.4

64

Zn 206

.200

Zn 213

.857

Zn 202

.548

Rel.

DL

2ml/min 21C.02ml/min 21C.02ml/min 60C

FEATURES OF ISOMIST

Programmable from -10 to 60oC in 1oC increments

Maintains temperature to within 0.1oC

Compact design (7.5x4x4 inches)

100% self-contained (no external lines)

Incorporates Bluetooth® technology for clean wireless control (USB available)

Compatible with all ICP-OES and ICP-MS models

CONSTANT TEMPERATURE BENEFITS

Higher accuracy Higher productivity Greater reproducibility Better experiments

(1mL/min sample uptake)