Tissue Culture

What is Tissue Culture ?

• Refers to technique of growing plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium

Media

• must be sterile• autoclave at 250 F at 15 psi

for 15 minutes• Murashige and Skoog media is commomly used

Explant• portion of plant removed and used for T.C.

Patterns of multiplication

• stage II - light 100-300 foot candles

• callus - shoots - roots• stage III - rooting - light intensity 1000-3000 foot candles

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