8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

1/22

9

Xamixa kcin gspk Ecpiki ~opsw &XaKGEc^/ IG7 Zpaxcod Napmw i Folfcp9

Aphcp Akolamcp idh Cdfidgcw IXZiwc igxo~oxy an Pczkogixoad Odoxoixap0

Zpaxcod &Pcz!IG9/7

8

Eikyid Esmip Ziwsmipxfy 9+ Dopszim Pay Gfashfspy 9+ Wsdok Esmip Msefcpbcc9 3

>

9Odxcpdixoadik Gcdxpc nap Lcdcxog Cdlodccpodl idh @oaxcgfdakaly+ Ipsdi Iwin Iko Mipl+ Dc|

9

Pcwskxw0

pczkogixoad$7

xpihoxoadik `pcchodl idh xpidwlcdog izzpaigfcw$ Daxch imadl xfcm ipc xpidwlcdog izzpaigfcw `iwch ad89

sdhcpcvzkapch hsc xa xfc honnogskxy iwwagoixch |oxf xfc zsponogixoad an waks`kc IG7 zpaxcod U07]$ Xa>0

`cxxcp sdhcpwxidh xfc `oagfcmogik gfipigxcpowxogw an xfc IG7 zpaxcod+ |c fi~c hc~ckazch i pa`swx>7

zpaeipyaxog cvzpcwwoad wywxcm nap Xamixa kcin gspk Ecpiki ~opswUOdhoi2Ecpiki OO20::3] &XaKGEc^5>8

HY630>07/ IG7 zpaxcod od C$ gako idh wxshoch oxw akolamcpog zpazcpxocw od waksxoad$ \c fi~c ikwa>3

cvimodch xfc odxcpigxoad an XaKGEc^ IG7 |oxf xfc galdixc IG9 zpaxcod idh xfc omzigx an xfow>>

odxcpigxoad ad xfc IXZiwc igxo~oxy an IG9$>6

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

4/22

8

>?

Pcwskxw idh Howgswwoad

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

5/22

3

?7

XaKGEc^ IG7 zpaxcod napmw i folfcp aphcp akolamcp?8

?3

Pczkogixoad ow i gamzkcv zpagcww xfix od~ak~cw odxcpigxoad |oxf ~ipoasw zpaxcodw$ Midy i xomcw wckn-?>

akolamcpowixoad an i zpaxcod lcdcpixcw mskxozkc woxcw xa odxcpigx ap odgpciwc xfc ipci an odxcpigxoad+?/+ |fogf ow zawwo`kc adky xfpaslf xfc odxcp-makcgskip odxcpigxoad `cx|ccd9:3

IG7 makcgskcw$ Xfaslf xfow cvzcpomcdx gappa`apixch |oxf xfc a`wcp~ixoadw xfix xfc XaKGEc^ IG79:>

akolamcpowch koec xfc dc| |apkh XLM^ IG7 U08]+ xfc akolamcpog wxixsw gaskh dax `c odncppch npam xfow9:

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

6/22

>

cvowxch iw id akolamcp |oxf i makcgskip miww folfcp xfid >>? eHi |fogf gappcwzadhw xa i folfcp aphcp99

cvzpcwwch od odwcgx gckk kodcw U08] idh xfc yciwx x|a fy`poh cvzcpomcdxw gippoch asx |oxf X_KG^ G7 idh97+ 7

IXZiwc igxo~oxy `y IG7 iwwsmcw woldonogidgc iw ox molfx odnkscdgc xfc IG9w fckogiwc igxo~oxy idh lo~cw i93:

9>9

Mcxfahw9>0

9>7

Gkadodl+ cvzpcwwoad idh zsponogixoad an pcgam`odidx M@Z idh LWX nswoad zpaxcodw9>8

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

8/22

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

9/22

?

Zsponoch LWX nswoad zpaxcod |iw odgs`ixch |oxf ~ipoch imasdxw an M@Z nswoad zpaxcod od `odhodl `snncp96?

U03 mM Xpow &zF 6$:/+

ZILC$ Xfc zpaxcod `idhw |cpc ~owsikorch `y |cwxcpd `kaxxodl ap gaamiwwoc `ksc wxiododl iw zcp wxidhiph9?]$ I`asx 03: ml an 0:0

cigf an xfc zspo ch zpaxcod |iw kiycpch hopcgxky ad i 9:$3 mk an 9:8:# &|!~/ wsgpawc wxcz lpihocdx od i0:7

`snncp gadxiododl 03 mM Xpow &zF 6$:/+ 03: mM DiGk+ 0 mM Wahosm `owskzfoxc idh :$:3# Xpoxad V-9::$0:8

Lpihocdxw |cpc gcdxponslch od i @cgemid W\89Xo paxap ix 73+::: pzm nap 0: fp ix 8G$ Npigxoadw &03: k/0:3

|cpc gakkcgxch idh ws`bcgxch xa 9:# WHWZILC$ Xfc zpaxcod `idhw |cpc ~owsikorch `y wok~cp wxiododl$0:>

Zpaxcod makcgskip miww mipecpw ~or$+ Ikhakiwc &936 eHi/+ Ncppoxod &88? eHi/+ Xfypalka`skod &>>? eHi/0:< ow xfc howxidgc npam xfc ivow an paxixoad xa xfc xaz an xfc gcdxponslc xs`c$ Pclpcwwoad idikywow swodl099

xfc Mogpawanx Cvgck izzkogixoad zpalpim yockhch xfc c sixoad2 y=73$8?:%0?$

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

10/22

9:

|fcpc y pczpcwcdxw xfc wchomcdxixoad howxidgc &od mm/ idh v pczpcwcdxw xfc makcgskip miww &od eHi/$097

Xfc wchomcdxixoad howxidgc nap M@Z-IG7 |iw xxch odxa xfc wxidhiph gsp~c idh xfcop dixo~c makcgskip098

miww |iw cwxomixch$093

09>

Lck Nokxpixoad09? eHi/+ Ncppoxod &88? eHi/+ Ikhakiwc &936 eHi/ idh000

Izpaxodod &? eHi/ |cpc swch iw makcgskip |colfx wxidhiphw sdhcp xfc wimc gadhoxoadw$007

008

IXZiwc Iwwiy003

00>

Xfc IXZiwc pcigxoad |iw gippoch asx `y odgs`ixodl xfc pihoaki`ckkch IXZ U9: Go an & -70Z/ IXZ &>:::00:

hspodl xfc cvzcpomcdxw$0>9

0>0

0>7

Pcncpcdgcw0>8

0>3

9$ I`fipy ME+ Idnaei LF+ Diefki ME+ Miv|ckk HZ2 Zawx-xpidwgpozxoadik lcdc wokcdgodl od0>>

gadxpakkodl ~opswcw an xfc Xamixa yckka| kcin gspk ~opsw gamzkcv$ Ipgfo~cw an ^opakaly 0::>+0>7$0>6

0$ Gfixxcpbo I+ @cigfy PD+ Nis scx GM2 Cvzpcwwoad an xfc akolamcporixoad hamiod an xfc0>?

pczkogixoad-iwwagoixch zpaxcod &Pcz/ an Xamixa kcin gspk Dc| Hckfo ~opsw odxcpncpcw |oxf HDI079-03>76$0

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

13/22

97

3$ Dapow C+ Iggaxxa LZ+ Xi~irri P+ @psdcxxo I+ Gpcwzo W+ Xi~irri M2Pcwowxidgc xa xamixa yckka|06:

kcin gspk lcmodo~opsw od Dogaxoidi `cdxfimoidi zkidxw xpidwnapmch |oxf i xpsdgixch ~opik G9069

lcdc$ ^opakaly 9??>+ 008297:-976$060

>$ Wfczfcph HD+ Midl|cdhc X+ Mipxod HZ+ @crsohcdfasx M+ Xfamwad BI+ Py`ogeo CZ2 Odfo`oxoad an 067

miorc wxpcie ~opsw &MW^/ pczkogixoad `y xpidwocdx idh xpidwlcdog cvzpcwwoad an MW^068

pczkogixoad-iwwagoixch zpaxcod msxidxw$ B Lcd opak 0::$063

xpsdgixch Pcz lcdcw npam Msdl`cid yckka| mawiog ~opsw-^oldi odfo`oxw ~opik pczkogixoad od06+ 7727>3-7

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

14/22

98

97$ Fiycw PB+ @sge E\2 Pczkogixoad an xamixa lakhcd mawiog ~opsw HDI @ od xpidwlcdog zkidxw7:0

cvzpcwwodl azcd pcihodl npimcw &APNw/ an HDI I2 pc sopcmcdx an APN IK0 nap zpahsgxoad an 7:7

wodlkc-wxpidhch HDI$ Dsgkcog Igohw Pcw9?6?+ 9

cnnogocdx HDI pczkogixoad$ ^opakaly 9??:+ 9

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

15/22

93

00$ Wcxxkilc W@+ Wcc PL+ Fidkcy-@a|haod K2Lcmodo~opsw G7 zpaxcod2 pczkogixoad cdfidgcmcdx idh70>

zpaxcod odxcpigxoadw$ B opak 0::3+ +

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

16/22

9>

79$ Lkihnckxcp FB+ Cilkc ZI+ Nadxcw CZ+ @ixxw K+ Fidkcy-@a|haod K2X|a hamiodw an xfc IK9 zpaxcod78?

mchoixc lcmodo~opsw apolod pcgaldoxoad$ ^opakaly 9??-9?

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

17/22

9

npigxoad iw odhogixch `y $ Ippa|w odhogixc xfc zcie napmixoad an makcgskip |colfx wxidhiph zpaxcodw27?xf npigxoad idh Xfypalka`skod &>>?eHi/ ix 7: xf 7?6

npigxoad$ Uoo] M@Z &87eHi/ hacw dax napm id akolamcp idh zciew od xfc 3 xf npigxoad$ &g/ Lck nokxpixoad |oxf7??

Wszcphcv-0:: 3!93: gakasmd wfa|w xfc cksxoad an ~ipoasw zpaxcodw$ M@Z-IG7 cksxcw `cx|ccd xfc8::

Hcvxpid &0:::eHi/ idh Xfypalka`skod &>>?eHi/$ 8:9

8:0

Nolspc 7$ LWX zskk-ha|d iwwiy nap od ~oxpa odxcpigxoad an XaKGEc^ LWX-IG7 idh M@Z-IG9$Gaamiwwoc8:7

`ksc wxiodch WHW-ZILC wfa|odl od ~oxpa odxcpigxoad `cx|ccd LWX-IG7 idh M@Z-IG9$ Npigxoadw8:8

gappcwzadhodl xa odzsx &kidcw 9-7/ pczpcwcdx xfc xaxik zpaxcod gamzawoxoad od cigf pcigxoad mov$ LWX-8:3

IG7 `asdh zpaxcodw |cpc xfapaslfky |iwfch xa pcma~c xfc dad-wzcgonogikky odxcpigxodl zpaxcodw$8:>

@asdh npigxoadw &kidcw 8->/ pczpcwcdx xfc zpaxcodw xfix |cpc odxcpigxodl |oxf LWX-IG7$ Zpcwcdgc an IG98:/ odhogixcw oxw odxcpigxoad |oxf IG7$8:6

8:?

Nolspc 8$ IG7 mahskixcw xfc IXZiwc igxo~oxy an Pcz$ &i/ Isxapihoalpizf wfa|odl xfc IXZiwc igxo~oxy an 89:

Pcz od xfc i`wcdgc idh zpcwcdgc an IG7$ IG7 odgpciwcw xfc IXZiwc igxo~oxy an Pcz ix ka| gadgcdxpixoad899

&:$:0-:$0 zM/ `y 3:-6:#$ Gamzawoxoad an xfc zpaxcodw od xfc pcigxoad mov ow wfa|d ix xfc xaz an cigf890

kidc od xfc isxapiholpizf$ IXZiwc pcigxoad |iw gippoch |oxf i sdonapm gadgcdxpixoad an Pcz zpaxcod idh897

~ipyodl gadgcdxpixoadw an M@Z-IG7 iw hcdaxch od xfc nolspc$ M@Z |iw swch iw i dclixo~c gadxpak$ &`/898

Lpizfogik pczpcwcdxixoad an IXZiwc igxo~oxy an Pcz od xfc zpcwcdgc an M@Z-IG7$ IXZiwc igxo~oxy od xfc893

pcigxoad mov gadxiododl xfc Pcz zpaxcod ikadc |iw ip`oxpipoky iwwoldch i ~iksc an 9::# idh igxo~oxy od89>

axfcp kidcw |iw gikgskixch iggaphodlky$ Lpizf |iw zkaxxch nap xfc kidcw 9-6 xfix gappcwzadh xa xfc kidcw89

0 3 6 99 98 9< 0: 07 0> 06 7: 70 78 7> 76 8: 80`$

Uo]

Uoo]

Npigxoad Da$

936 eHi 88? eHi >>? eHi

:$: 9$: 0$: 7$: 8$: mk

Hcvxpid&0::: eHi /

M@Z-IG7

Xfypalka`skod&>>? eHi /

Izpaxodod&>$? eHi /

mIs

73:

7::

03:

0::

93:

9::

3:

:

g$

Nolspc 0

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

21/22

M@Z-IG9

M@Z

LWX-IG7

Odzsx @asdh

99>

>>

83

eHi9 0 7 M 8 3 >

Nolspc 7

8/9/2019 Tomato leaf curl Kerala virus (ToLCKeV) AC3 Protein Forms a Higher Order Oligomer and Enhances ATPase activity o

22/22

`$

0::

03:

i$

- :$9 :$9 :$9 :$9 :$9 :$9 :$9 - :$9 - - -

- - - - - - - - :$9 :$9 - - -M@Z &zmakc/- - :$:0 :$:> :$9 :$0 :$7 :$3 - - :$:0 :$0 :$3M@ZIG7 &zmakc/

9 0 7 8 3 > < 6 ? 9: 99 90 97

Pcz &zmakc/

Z70Ki`ckkch IXZ

Pckciwch Z o

i g x

o ~ o x y

:

3:

9::

93:

9 0 7 8 3 > < 6

M@ZE0QIG7 &zmakc/

Pcz &zmakc/ - :$9 :$9 :$9 :$9 :$9 :$9 :$9

- - :$:0 :$:> :$9 :$0 :$7 :$3

P c

k i x o ~ c

& # / I X Z i w c

Nolspc 8