Toward the industrialization of Okinawa biological resources
Name Shinya Ikematsu E-mail [email protected]
Status Vice-President ( Research affairs ), Professor
Affiliations
JBS (The Japanese Biochemical Society), MBSJ (The Molecular Biology Society of Japan), JCA (The Japanese Cancer Association), JSPHO (The Japanese Society of Pediatric Hematology/Oncology), JSEE (Japanese Society for Engineering Education), JSOG(The Japan Society of Obstetrics and Gynecology), JSBBA(Japan Society for Bioscience, Biotechnorogy, and Agrochemistry) and JSBi (Japanese Society for Bioinformatics)
Keywords Biological resources,・Regenerative medicine,・lactic acid bacteria,・Development of new
technology,・Functional materials,・Pharmaceuticals,・Cosmetics,・In-vitro diagnostics
Technical Support Skills
・ General analysis- material analysis and gene analysis (including Bioinformatics) ・ Process management, mainly GMP ・ Development of human resources (Bioinformatics technician, Clinical embryologist )
Available Facilities and Equipment
DNA sequencer, CEQ8800 (Beckman Coulter) NGS, MiSeq (illumine) HPLC & UPLC/MS (Waters) Cell sorter Fluorescence microscope, Axio Imager Z2 (Carl Zeiss Microscopy) Safety cabinet
Realtime PCR, 7500 (Applied Biosystems) FPLC AKTA explorer (GE healthcare)
Department of Bioresources Engineering
National Institute of Technology, Okinawa College
Research
contents:
Development and application of cell-free protein synthesis system
Name ITO Masaaki E-mail [email protected]
Status Professor
Affiliations Japan Society for Bioscience, Biotechnology, and Agrochemistry. The Japanese Biochemical Society
Keywords Cell-free protein synthesis system, insect, silkworm, polyphenol oxidase
Technical
Support Skills
・ Recombinant DNA experiment
・ Screening, purification and analysis of enzymes
Research Contents Development and application of insect cell-free protein synthesis system
Insect cell-free protein synthesis system
Available Facilities and Equipment
Spectrophotometer, DU800(Beckman Coulter) Microplate Reader, infinite M200(TECAN)
Spectrophotometer, DU800(Beckman Coulter) Microplate Reader, sunrise rainbow thermo(TECAN)
Chromatography system, AKTA avant(GE Healthcare) Microchip Electrophoresis System, MultiNA(SHIMADZU)
Chromatography system, AKTA prime plus(GE Healthcare)
Cell disruptor, MULTI-BEADS SHOCKER(YASUI KIKAI)
KOSEN National Institute of Technology
Department of Bioresuorces Engineering
National Institute of Technology, Okinawa College
Name Junsei Taira E-mail [email protected]
Status Professor
Affiliations ・The Pharmaceutical Society of Japan ・American Chemical Society
Keywords ・Oxidative stress, Antioxidant, Cosmetics, Functional food
Technical
Support Skills
・Evaluation of functional materials in bioresources
・Isolation of functional compounds
Research Contents
My research target is to find the active compounds as potential useful functional food, pharmaceutical
cosmetics and drugs in bioresources (plants1-4) and marine natural products5-6) and also make the aroma
profiles in beverages 7). The research flow indicated in the under figure and general evaluation is as follows.
Polyphenol content, antioxidant activity (ORAC method, ESR study), anti-inflammatory effect, antitumor
activity, apoptoic activity and inhibition of melanogenesis.
Recent research publications are as follows.
1) Oxidants and Antioxidants in Medical Science. 2, 21-28, 2013
2) Journal of Food Composition and Analysis. 29, 117-125, 2013
3) Journal of Agricultural and Food Chemistry. 62, 167−172, 2014
4) Food Chemistry. 166, 531-536, 2015
5)Marne Drugs. 10, 2741-2748, 2012
6) Tetrahedron Letters. 55, 1421-1423, 2014
7) Food Science and Technology Research. 18, 177 -181, 2012
Available Facilities and Equipment
Large scale headspace GC/MS Agilent Technologies
LC/MS Agilent Technologies
LC for analysis and preparative isolation Agilent Technologies
DNA analyzer Agilent Technologies
Department Bioresources Technology
National Institute of Technology, Okinawa College
Find Assay
Analysis
Natural products
KOSEN National Institute of Technology
Development of Possible Applications for Local Food Resources
Name TANAKA Hiroshi E-mail [email protected]
Status Professor
Affiliations Japanese Society for Bioscience, Biotechnology, and Agrochemistry
The Japanese Society for Food Science and Technology
Keywords Local resources, Lactic acid bacteria, Food processing, Marketing
Technical
Support Skills
・ Preparation of foods and/or ingredients
・ Nutrient analysis and functionality evaluation
・ Functionality of lactic acid bacteria
・ Development of marketing plan for new products
Research Contents Converting local natural resources to food ingredients with added values
1. Development of food processing methods utilized by original microbes
Development of food processing methods by lactic acid bacteria isolated in Okinawa
Isolation of local marine lactic acid bacteria with unique properties
Identification of isolated lactic acid bacteria by sequencing of 16S rRNA
Screening of antimicrobial activity of isolated local lactic acid bacteria
Characterization of isolated lactic acid bacteria for their fermentation properties
2. Utilization of local botanical resources
Construction of an in vitro assay method for induction activity of detoxification enzymes
Screening of extracts of local vegetables and fruits for the induction activity
Purification and identification of active substance(s) of the induction activity
Characterization of Ryukyu-Ai (Strobilanthes cusia) extracts for antimicrobial and antiviral activities
3. Utilization of local animal resources
Evaluation of goat milk for its food processing characteristics
Development of original goat cheese with new rheological properties
Analysis of rheological properties of goat milk
Development of goat milk based alcoholic beverages with fermentation
Analysis of aroma constituents by GCMS
4. Marketing strategy
Development of marketing strategy for utilization of local natural resources
Consultation of marketing method for new product development
Available Facilities and Equipment
Lactic acid bacteria culture system
UPLC (Waters)
Cell culture system
Cooking laboratory
Marketing mix
Department of Biorsources and Engineering,
National Institute of Technology, Okinawa College
Bioresources and Photochemistry
Name HAMADA Taisuke E-mail [email protected]
Status Professor
Affiliations
The Chemical Society of Japan, Japan Society of Coordination
Chemistry, The Electrochemical Society of Japan, Japanese Society
of Engineering Education
Keywords Photochemistry, Physical chemistry, Coordination chemistry
Technical
Support Skills
・ Measurement of photochemical property
・ Supercritical fluid extraction
・ Photo energy conversion
Research Contents
[Dye sensitized solar cell]
Solar energy have attracted much attentions because it is clean, and it is called green energy. Solar cell
is device to convert photo-energy to electric energy. We have been studied a solar cell, which is called the
dye sensitized solar cell (DSSC).
We have made DSSCs and studied the effect of the dye on the efficiency under irradiation of sunlight (AM
1.5G) from the solar simulator (Fig. 1). In this study, we have evaluated the photochemical and
photoelectrical properties of the dye. Therefore, we are able to measure and to evaluate the photochemical
property of various compounds in order to clarify a photochemical ability.
[Super critical fluid extraction]
We have been trying to obtain the useful compound such as poly-methoxy flavonoid (PMF) by means of
super critical CO2 extraction method. The merit of this method is that the temperature for the extraction
is relatively low and CO2 dose not remain in an extract.
Available Facilities and Equipment
Solar simulator (WACOM)
Supercritical CO2 extraction apparatus (AKICO)
Department of Bioresources Engineering
Okinawa National College of Technology
KOSEN National Institute of Technology
Department of Bioresources EngineeringNational Institute of Technology, Okinawa College
Research of the Okinawan traditional distilled liquor
“AWAMORI”
Name Yasutomo Yamaki E-mail [email protected]
Status Associate Professor
Affiliations
Japan Society for Bioscience, Biotechnology, and Agrochemistry
The society for Biotechnology, Japan
The society of Tropical Resources Technology
Keywords Awamori, fermentation, microoganizms, Black koji-mold,
Technical
Support Skills
・ The whole about the AWAMORI. / ・Aromatic component analysis.
・ Alchoholic beverages development of products. / ・Fermented food development.
Research Contents Research of the microbe which has an influence on the quality of Awamori.
Koji
MoromiSampling
Isolation ofmicrooganisms
Experimental brewing
Identification ofmicrooganisms
Preservation ofmicrooganisms
Analysis of qualityof liquor
Elucidation ofthe function
Develotment ofnew Awamori
Awamori
Establishment ofbrewing method
Available Facilities and Equipment
名称・型番(メーカー)
Gas Chromatograph Mass Spectrometer (GC-MS : Shimadzu) Headspace automatic sampler system (Perkin Elmer)
High Performance Liquid Chromatography (HPLC : Shimadzu) Biophotorecorder (ADVANTEC)
Differential scanning calorimeter (DSC : Shimadzu) Thermal Analysis (TA : Shimadzu)
Jar fermenter ( Takasugi) Clean bench (AIRTEC)
Autoclave (Tomy) Thermo-hygrostat (EYELA)
Development of functional food materials from subtropical bioresources
Name Takahiro MITSUE E-mail [email protected]
Status Professor
Affiliations
Japan society for food science and technology., Japan society for Biotechnology., Japan society for Bioscience, Biotechnology, and Agrochemistry.
Keywords Microorganisms, Food product., Quality control
Technical
Support Skills
・ Fermentaion of every materials
・ Establishment of manufacturing process
・ Quality control (ISO, HACCP)
Research Contents
Biochemical scientists are expected to solve the problems of life
science, use of energy and bioresources, and surroundings.
Nowadays, it is possible to produce new biomaterials with excellent
functions.
Also, we are trying to develop the functional food or medical
materials from subtropical bioresources in Okinawa.
Available Facilities and Equipment
HPLC (Shimazu)
Jarfermentor (Takasugi)
Microscope (SEM,Optical)
Capillary for electrophoresis (Agilent)
Color difference meter (Konica)
KOSEN National Institute of Technology
Department of Bioresources Engineering
National Institute of Technology, Okinawa
College
Available Facilities and Equipment
Capillary Sequencer: CEQ8800 (Beckman Coulter) Next Generation Sequencer MiSeq (illumine)
Search for biodiversity and function of marine organisms and application to conservation
Name Naoko Isomura E-mail [email protected]
Status Assistant Professor
Affiliations The Zoological Society of Japan, The Ecological Society of Japan, Japanese Coral Reef Society
Keywords Corals, Reproduction, Reproductive ecology, Genetic differentiation, Speciation
Technical
Support Skills
・ Genotyping using sequences and microsatellite marker
・ Collecting gametes, cross experiments and rearing juveniles of marine organisms
・ Collecting marine organisms by SCUBA
・ Microbial community analysis by NGS (not main theme)
Research Contents
In my study, my purposes are to search biodiversity of marine organisms, especially scleractinian corals,
and to apply for conservation.
My purpose is to estimate the genetic diversity and its function of marine organisms in Okinawa. First, I
will try to clear morphological and/or diversity of species and population. And then, after I search and
clarify the endocrine system of organisms by physiological and molecular analyses, I will evaluate the
relationship between these system and ecological characteristics of organisms. Moreover, I will investigate
conservation method for target organisms using my results.
KOSEN National Institute of Technology
Department of Bioresuorces Engineering
National Institute of Technology, Okinawa College
Cloning of the gene for plant stress responsiveness
Name SANMIYA Kazutsuka E-mail [email protected]
Status Associate professor
Affiliations The Japanese Society of Plant Physiologists The Molecular Biology Society of Japan
Keywords plant stress responsive gene, isoprenoid, heat shock protein, saponin
Technical
Support Skills
・ Gene cloning
・ Gene expression analysis
・ Transgenic plant
Research Contents FPPS gene, heat shock protein gene, UGT gene, Rice yield
● Cloning of the gene for FPPS
For the first time, the gene for chloroplast FPPS was cloned. FPPS is the key enzyme for isoprenoid biosynthesis.
Isoprenoids play roles in plant stress response such as protection against sunlight.
● Heat tolerant transgenic tobacco The gene for heat shock protein was used for the transformation (Fig).
● Cloning of the gene for UGT
For the first time, the gene for saponin biosynthesis was cloned. Saponin protects the Brassicaceae crops, cabbage, radish, broccoli against pests.
● Rice yield increased to 124% by glass silica
Available Facilities and Equipment
Electrophoresis apparatus Mupid (ADVANCE)
Gene expression analyser AE6933 (ATTO)
Thermal cycler TP450 (TaKaRa)
Department of Bioresources Engineering
National Institute of Technology, Okinawa College
wild type
tobacco
heat tolerant
transgenic tobacco
KOSEN National Institute of Technology
Research Contents
Food processing, non-thermal sterilization and high efficiency extraction using the shock wave
Name Takemoto, Ayumi E-mail [email protected]
Status Associate Professor
Affiliations
The Japan Society of High Pressure Science and Technology
The Japan Society of Cookery Science
The Materials Research Society of Japan
Keywords Shockwave processing, Flouring, Extraction, Non-thermal sterilization
Technical
Support Skills
・ Shock wave processing to biosystem
・ Non-thermal milling, Efficient extraction
Available Facilities and Equipment
Rheometer , RE2-33005C (YAMADEN co.,ltd)
Department of Bioresources Engineering
National Institute of Technology, Okinawa College KOSEN National Institute of Technology
Application of Okinawan microorganisms
Name Toshiaki TANABE E-mail [email protected]
Status Associate Professor
Affiliations JSBBA, SBJ, JSMSB, JSCC, MSJ, and BSO
Keywords Chitin, Chitosan, White-rot fungi,
Technical
Support Skills
・ Component analysis of biomass
・ Screening of bacteria or enzymes for bioremediation or bioconversion
Research Contents
Construction of Okinawan Soil library
The soil samples were collected from approximately 2,600 places including each remote island in
Okinawa, and were constructed to the Okinawan soil library. Various kinds of microbes are isolating from
this library. They have various bioactive substances and enzymes.
Construction of the soil microbe library
The soil microbe library was constructed with each microbe group isolated from each soil sample. This
library includes about 500 strains of bacteria utilizing alkane, 500 strains of siderophore-like
material-producing bacteria, 1,700 strains of Actinomyces, 500 strains of Basidiomycetes.
Application of biologically active substances
Bioactive substance library has been constructed from the extracts of the soil microbe. For example,
siderophore is an iron chelating agent which microbe produce. It is thought that siderophore is able to apply
for anticancer.
Application to bioremediation
The isolated bacteria utilizing alkane are able to use for cleaning of the soil polluted with oil. In addition,
the white-rot fungi degrading high selectively lignin are able to remediate the soil polluted by dioxin or
PCB.
Application to hydrogen production
The biomass conversion from molasses, or waste of plants, to hydrogen is paid attention now. The isolated
microbes producing hydrogen are used to constract a hydrogen production system.
Available Facilities and Equipment
Bioshaker G B-200(Taitec)
Electrophoretic apparatus(GE Healthcare Japan)
Department of Bioresources Engineering,
National Institute of Technology, Okinawa College KOSEN National Institute of Technology
Research Contents
○Background and Purpose
In order to solve energy and environmental problems, I am trying
to construct the efficient synthesis process for biomass energies
such as biodiesel and bioethanol and the conversion of waste
materials to useful chemical compounds.
○Researches
Theme 1: The development of solid catalysts through mechanochemical treatments using ball mills
In the theme, the development of preparation method of
effective solid catalysts from magnesium oxides and zirconium
oxides for biodiesel synthesis through the mechanochemical
treatments using ball mills has been tried. In addition, I am
trying to develop the reaction process for the conversion of
glycerol, which is a by-product of biodiesel synthesis, to the useful
chemical compounds such as lactic acid and acrylic acid.
Theme 2: The continuous synthesis of biodiesel using a column reactor
packed with solid catalysts
The efficient synthesis process of biodiesel fuel from vegetable
oils using a simulated moving bed chromatographic reactor is
studied.
Theme 3: The hydrothermal conversion of biomass resources to useful
chemicals under high temperature and high pressure
condition
The hydrothermal conversion of biomass resources such as
bagasse and glycerol to useful chemical compounds is studied.
Biodiesel
Glyceol
Catalyst
Feed( Oil + Methanol)
Reactionproducts
Development of efficient catalysts through mechanochemical treatment
Development of efficient reaction system
OilAlcohol
Biodiesel fuel
Glycerol
Lactic acid
High temperature and high pressure reactor
Research on the conversion of waste glycerol to useful chemical
Biodegradable polymer
Packed bed reactor
250℃~350℃
Glycerol(Biomass resources)
Lactic acid(Reactant of biodegradable polymer)
Acrylic acid(Reactant of superabsorbent polymer)
Research on the Development of Efficient Reaction Process for Biomass Conversion
Name Takuya Fukumura E-mail [email protected]
Status Doctor of Engineering
Affiliations
The Society of Chemical Engineers, Japan
The Japan Institute of Energy Japanese Society for Engineering Education
Keywords Biomass energy, Low Environmental Load, Catalysis
Technical
Support Skills
・ Synthesis of biodiesel fuel and bioethanol
・ Applications of inorganic chemicals
・ Effective use of biomass resources
Available Facilities and Equipment
KOSEN National Institute of Technology
Department of Bioresuorces Engineering
National Institute of Technology, Okinawa College
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Biodiversity of Oribatid mites in Ryukyu Islands
Name Wataru HAGINO E-mail [email protected]
Status Assistant Professor
Affiliations The Acarological Society of Japan, The Japanese Society of Soil Zoology, Japanese Society of Systematic Zoology
Keywords Soil fauna, Oribatid mites, Biological indicator, Ecology, Taxonomy
Technical Support Skills
ð Taxonomical study of Oribatid mites (Order Acari; suborder Oribatida). ð Development of the biological indicator used by soil organisms. ð Experience-based program on biodiversity of our familiar environment.
Research Contents Oribatid mites (Fig. 1) are small arthropods that are also called beetle mites, armored mites, or moss mites. They comprise more than 10,000 species. Most oribatid mites inhabit the soil–litter system; often, they are the dominant arthropods in temperate-forest soil. The ultimate goal of my research is to clarify the entire Japanese oribatid fauna. Toward this goal, I performed a series of taxonomic studies targeting the Oribatid fauna of subtropical regions in Japan (=Ryukyu Islands), by using morphological and molecular biological methods.
(Fig. 1) Various Oribatid mites in Japan.
Available Facilities and Equipment
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