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TRACE ELEMENT KINETICS IN CONTAMINATED POSIDONIA OCEANICA MEADOW
J. Richir , N. Luy , G. Lepoint, A. Alvera Azcarate, R. Biondo, S. Gobert
Essaouira05-2012
Mediterranean submitted to anthropic pressures
Introduction
INTRODUCTION
bioindicator : Posidonia oceanica- monitoring of coastal ecosystem pollution;- representative accumulation;- pollutant biologically accessible.
pollution trace elements (TEs)
TE Cd Pb Cu Zn Cr Fe Ni
# ref. 39 30 28 26 23 15 14
TE As Ag Co Mn Al Bi V Mo
# ref. 4 3 3 3 2 1 1 1
Luy et al. (2012)
Mediterranean submitted to anthropic pressures
Introduction
INTRODUCTION
bioindicators : Posidonia
oceanica
pollution trace elements (TEs)
TEs uptake and loss kinetics of P. oceanica contaminated in their natural environment?
e.g.: Ledent (1992); Warnau et al. (1996)
Mediterranean submitted to anthropic pressures
Introduction
INTRODUCTION
bioindicators : Posidonia
oceanica
pollution trace elements (TEs)
TEs uptake and loss kinetics of P. oceanica contaminated in their natural environment?
Objectives:
To study TEs kinetics and tissue compartmentalization of P. ocenica contaminated in situ at 2 experimental levels:
- moderate: 21x Calvi SW conc.; 4x world ocean mean conc. - acute: 151x Calvi SW conc.; 31x world ocean mean conc.
e.g.: Ledent (1992); Warnau et al. (1996)
In situ experimental contaminations
MATERIAL - METHODS
MATERIAL
-
METHODS
Contamination at moderate levels
• 5 days of contamination in June 2009;
• 410L bell-shaped mesocosm;
• Contamination every 12 hours (9am-9pm);
• 15 days of decontamination.
MATERIAL
-
METHODS
Contamination at moderate levels
MATERIAL
-
METHODS
Contamination at acute levels
• 24h00 of contamination in June 2009;
• 54L rectangular-box mesocosm;
• 5 contaminations, 1 every 3 hours (9am-9pm);
• 15 days of decontamination
Posidonia oceanica
(A) shoot of leaves on a plagiotropic rhizome (Rz); (B, C) adult leaves (AL); (D) intermediate leaf (IL); (E) juvenile leaf. Libes and Boudouresque,1987
plagiotropic shoot
orthotropic shoot
rhizome
blade
roots
MATERIAL
-
METHODS
limb
ligula
leaf base (petiole)
EDCB
A
rootleaf base
stipule
scale
rhizome
RESULTS
Ag moderate conta.: kinetics
RESULTS
Ag moderate conta.: kinetics
RESULTS
Ag moderate conta.: kinetics
RESULTS
Ag moderate conta.: kinetics
RESULTS
Ag moderate conta.: compartmentalization
RESULTS
Ag moderate conta.: compartmentalization
RESULTS
Ag moderate conta.: kinetics
RESULTS
Ag acute conta.: kinetics
RESULTS
Ag acute conta.: kinetics
RESULTS
Ag acute conta.: kinetics
RESULTS
Ag acute conta.: compartmentalization
RESULTS
Ag acute conta.: compartmentalization
RESULTS
Ag acute conta.: kinetics
CONCLUSION
Conclusion
• P. oceanica takes up TEs from the beginning of experiments;
• Moderate contamination design are preferred;
• Once contaminations end, TE concentrations rapidly decrease;
• Leaves exhibited different uptake kinetics: the younger growing leaves forming new tissues incorporated chemicals more rapidly than the older senescent leaves;
• Rhizomes did not show any clear trend.
P. oceanica is a sensitive sentinel to delineate punctual pollutions similar to what might be measured in contaminated Mediterranean waters routine use in regularly scheduled monitoring programs.
QUESTIONS
Thank you for your attention
RESULTS
Cu moderate conta.: kinetics
RESULTS
Cu moderate conta.: compartmentalization
RESULTS
Cu moderate conta.: kinetics
RESULTS
Cu acute conta.: kinetics
RESULTS
Cu acute conta.: compartmentalization
RESULTS
Cu acute conta.: kinetics
RESULTS
Fe moderate conta.: kinetics
RESULTS
Fe moderate conta.: compartmentalization
RESULTS
Fe moderate conta.: kinetics
RESULTS
Fe acute conta.: kinetics
RESULTS
Fe acute conta.: compartmentalization
RESULTS
Fe acute conta.: kinetics
Analytical steps:
• homogeneous sample
• acidic digestion in a microwave oven
• measures : inductively coupled plasma mass spectrometer
MATERIAL
-
METHODS
Laboratory analyses
Ethos D
ELAN DRCII
adult leavesshoots 1/6/9
Calvi