TSQ ENDURA Vent and Clean Procedure

The instructions provided should be used to supplement the official hardware manual for the TSQ Endura.

What you will need:

Sonicator BathDi H20LCMS Grade H20LCMS Grade MethanolClean Glass BeakersLiquidnox

Vent the system

Make sure you are not running any samples and there is no flow into the mass spec.

Click the “off” button in the Endura tune program

Set the electronics to service mode by flipping the switch to the down position. The switch is located on the right side of the instrument. (Figure 1 - A)

Pull the main power switch down to the off position (Figure 1 - B). The Mass Spec will now be vented and in 2 mins it will be safe to proceed, but it would be best to wait till the source has cooled down. Disconnect the power cord from the back and proceed to remove the source.

Remove the Source

Make sure to use clean gloves to remove the source, sweep cone and ion transfer tube. If you don’t know how to do this, consult a more qualified person to complete the rest of the steps. After they have been removed your source should look like this (Figure 2).

Figure 1: Right Side of the Endura

Figure 2: Ion Source, sweep cone and transfer tube removed

Remove the ion source interface

Flip the release lever up. You will need to use significant force to push the ion source interface out from the source mount. If you think you have loosen it by using minimal force, then you are wrong, you really need to crank it. Refer to Figure 3 (not loose enough) and Figure 4 (fully released) and pay attention to the position of the lever and there should be a gap between the ion source interface and the ion source mount on all sides.

Figure 3: Interface not fully released Figure 4: Fully released interface

Once you crank the lever enough, remove the ion source interface and place onto a lint free cloth. Handle with care (Figure 5).

Remove the MP00 multipole and L0 lens assembly

Loosen and extend the Retainer Thumbscrews that is securing the assembly onto the ion interface, refer to figure 6. Once that is done, the MP00/L0 lens assembly should just fall out (Figure 7). Handle with care and place on cloth.

Figure 5: Removed Ion interface

Removing the RF and exit lenses from the ion source cage

Once fully extended, the retaining thumbscrews are free to move around, which is not good. While fully extended, loosen the thumbscrews 2 – 2.5 times more to engage the threads. This will allow for much more leverage, as it is near impossible to remove the RF and exit lenses from the ion source cage without doing this step.

With the Thumbscrews engaged, proceed to pull out the RF and exit lens. It should pop out (Figure 8). Remove the exit lens by turning it upside and the exit lens should slide out.

Figure 6: Thumbscrew securing MP00 and L0 lens assembly

Figure 7: MP00 and L0 assembly

Figure 8: RF and exit lenses ready to be removed

Figure 9: RF and Exit Lenses

Disassemble the MP00 multi-pole and L0 lens from its mount cage

Begin by removing the L0 lens by rotating it so the slots line up with the copper clips on the side. It should now be easy to remove the L0 lens, refer to figure 10.

Next, pull the MP00 out of the mount cage. You should now have all three parts disassembled (Figure 11).

Clean and Sonicate Parts

Place all the parts into clean glass beakers. Place the RF lens in one beaker and the rest in another beaker.

Prepare a 1% solution of Liquidnox in DI H20 and make enough to fill both beakers. Make sure all parts are emerged in the Liquidnox solution (Figure 12).

Sonicate the Parts for 10-15 mins. For each part sonicated, remove from beaker and rinse with copious amounts of tap water. The tap water will ensure that no soap residue remains. Once rinsed thoroughly with tap water, place the parts into clean beakers, fill with LCMS grade H20 and sonicate again for 10-15 mins.

Next, drain the LCMS h20 water and fill with LCMS grade Methanol and sonicate again for 10-15. Repeat the step one more time with fresh methanol.

Figure 10: L0 lens

Figure 11: Disassembled MP00 and L0 lens

Figure 12: Parts in 1% Liquidnox

Complete the process by drying the parts under nitrogen gas. Place all parts for reassembly on clean, non-lint cloth.

Reassemble Parts

Reassembly the MP00 Multipole by aligning the slot it on the mount cage (Figure 13). Push in until it snaps into place.

Take the L0 lens, place it on the mount cage and rotate the lens 90 degrees to secure it in place (Figure 14).

Reassembly the Ion source interface by aligning the three pins on the back of the RF lens, to the three slots on the Ion source cage (Figure 15). Push until it snaps in place.

Install the Exit Lens to the front of the RF lens by guiding the contact pin into the hole as shown in Figure 16.

Align the guide pin on the exit lens to the guide pin hole on the back of the MP00 and L0 assembly. Secure together by hand tightening the retainer thumbscrews (Figure17).

Everything should now be reassembled (Figure 18).

Figure 13: MP00 Alignment

Figure 14: L0 Lens Alignment

Figure 15: Ion cage and RF lens pin alignment

Figure 16: Exit Lens Contact Alignment.

Figure 17: MP00/L0 lens assembly installed

Install the ion source interface and start pump down

Place the Ion source interface back onto the mass spec. Carefully guide it in place and push it in to seat it completely. Do not install the ion transfer tube or the Sweep cone yet. This will speed up the pump down process. Install the source, plug the power cord back in, flip the main power back on and switch the electronics to operation. A series of self-tests will occur, wait at least 1 hour before installing the iontransfer tube and then allow the mass spec to pump down over night. After that process, a full calibration on both positive and negative modes should be done before it is ready for duty.

Figure 18: Complete Ion Source Interface reassembled