©2017 Waters Corporation 1COMPANY CONFIDENTIAL
Ultimate Efficiency Unleashed:
Introducing CORTECS™ Solid-Core Particle Columns for
Maximizing Resolution and Throughput
Willem Joubert
Microsep
Waters Division
©2017 Waters Corporation 2COMPANY CONFIDENTIAL
Agenda
Review of Solid-Core Particles
CORTECS® Solid-Core Particle Columns
Column Benefits for Improved Laboratory Performance
Practical Applications of CORTECS Columns
Summary
©2017 Waters Corporation 3COMPANY CONFIDENTIAL
Review of Solid-Core Particles
Solid-Core particles have been around since the 1970’s
– Waters Corasil I 30-80 µm particles
– Poor efficiency and poor loading capacity
Today's modern core-shell particles are prepared by the multi-layering of
silica sols around a solid silica core.
– Thicker shell, allows for better Loading capacity
– Better particle design, improvements in packing provide higher efficiency
FIB SEM ImagesSolid-Core
Core
Part
icle
©2017 Waters Corporation 4COMPANY CONFIDENTIAL
The Solid-Core Particle
Only thin outer layer contains the pores
with the chromatographic surface
The center core is nonporous
The outer shell is typically “bumpy” not
pretty
The particle size distribution is narrower
CORTECSSolid-core
dcore = 1.1 µm
dp
= 1
.6 µ
m
Rho, r = 1.1/1.6 = 0.7
66% Porous Volume
ρ = 0 → fully porous particle
ρ = 1 → nonporous particle
ρ = core diameter / particle diameter
Compared to Fully Porous Particles
G. Guiochon, F. Gritti, J. Chromatogr. A 1218 (2011) 1915–1938 Omamogho et al., J. Chromatogr. A 1218 (2011) 1942-1953
©2017 Waters Corporation 5COMPANY CONFIDENTIAL
Why are Modern Solid-Core Columns so
Popular?
Provide higher efficiency when compared to fully porous particles of
equivalent particle size.
Provide lower backpressure when compared to fully porous particles of
equivalent particle size.
Efficiency
Pressure
©2017 Waters Corporation 6COMPANY CONFIDENTIAL
Achieving Optimal Efficiency
Impact of System Dispersion
The Effect of System Dispersion on Column Performance
– In 2004, key component of UPLC introduction was the relationship between
observed efficiency of small particle columns and the dispersion of the system
– As system dispersion decreases, observed efficiency of columns increases
– In order to realize full efficiency of CORTECS Columns, match the column
dimension and particle size with the instrument dispersion
©2017 Waters Corporation 7COMPANY CONFIDENTIAL
Where Does System Dispersion Occur?
Band Spreading: 1) From the Injector2) Into, through and out
of the column3) Into the Detector
Extra ColumnWithin Column
©2017 Waters Corporation 8COMPANY CONFIDENTIAL
Band Spreading, Peak Height, and
Resolution
LC systems (column and instrument) capable of producing narrower/sharper bands create narrower/sharper peaks
This results in better resolution, taller peaks and better sensitivity
Better separationMore concentrated “Bands”Higher Sensitivity
Both analytes (blue and red) are not separated [a partial co-elution –shown as a “purple” band]
System withMORE
Band Spreading
System with LESS
Band Spreading
©2017 Waters Corporation 9COMPANY CONFIDENTIAL
System Dispersion Influence Across Different
Column i.d.s
AU
0.00
0.02
0.04
0.06
AU
0.00
0.02
0.04
0.06
AU
0.00
0.02
0.04
0.06
Minutes
0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.00 1.10 1.20 1.30 1.40 1.50
4.6 x 50 mm
3.0 x 50 mm
2.1 x 50 mm
7200
4800
2900
Efficiency
3.6
2.5
1.4
Alliance HPLC: Bandspread 36 µL
Resolution
Using a larger column I.D. (higher column volume) mitigates the effect of system dispersion on a separation
©2017 Waters Corporation 10COMPANY CONFIDENTIAL
Matching System Dispersion With Column I.D.
AU
0.00
0.02
0.04
0.06
AU
0.00
0.02
0.04
0.06
AU
0.00
0.02
0.04
0.06
Minutes
0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 0.90 1.00 1.10 1.20 1.30 1.40 1.50
4.6 x 50 mm
3.0 x 50 mm
2.1 x 50 mm
HPLC: AllianceDispersion: >30 µL
UHPLC: ACQUITY ArcDispersion: 12-30 µL
UPLC: ACQUITY UPLC H-ClassDispersion: <12 µL
7200
7600
7300
Efficiency
3.6
Resolution
3.8
3.7
Matching column I.D. (column volume) to system dispersion helps to maintain column efficiency and resolution
©2017 Waters Corporation 11COMPANY CONFIDENTIAL
Matching System Dispersion With Column I.D.
Dispersion > 30 µL
Columns accepted: • 3.0 – 4.6 mm ID• 3 - 10 µm particles
Recommended: • 4.6 mm ID, 3.5 or 5 µm
Typical operating pressure: • < 6,000 PSI
Dispersion 12 - 30 µL
Columns accepted: • 2.1 - 4.6 mm ID• 1.7 - 5 µm particles
Recommended: • 3.0 mm ID, 2.x µm
Typical operating pressure: • 6,000 – 15,000 PSI
Dispersion < 12 µL
Columns accepted: • 1.0 - 4.6 mm ID• 1.6 - 5 µm particles
Recommended: • 2.1 mm ID, 1.x µm
Typical operating pressure: • 9,000 – 15,000 PSI
Increased flexibility and sample characterization
©2017 Waters Corporation 12COMPANY CONFIDENTIAL
What makes Solid-Core Particles Different from
Fully Porous?
Lets look into the science of how to get lower backpressure and higher
efficiency
– Backpressure equation
– Improved efficiency
o - the van Deemter equation
F. Gritti, G. Guiochon, J. Chromatogr. A 1221 (2012) 2– 40F. Gritti, Chromatography Today May/June (2012) 4-11
©2017 Waters Corporation 13COMPANY CONFIDENTIAL
What makes Solid-Core Particles Different from
Fully Porous?
Lets look into the science of how to get lower backpressure and higher
efficiency
– Backpressure equation
– Improved efficiency
o - the van Deemter equation
F. Gritti, G. Guiochon, J. Chromatogr. A 1221 (2012) 2– 40F. Gritti, Chromatography Today May/June (2012) 4-11
©2017 Waters Corporation 14COMPANY CONFIDENTIAL
Lower Backpressure? How?
Pre
ssure
(psi)
External or Interstitial Porosity, ee
3
2
22
)1(180
e
e
pdr
LFP
e
e
Kozeny-Carman Equation
The effect particle size, dp, and
the external porosity, ee , on
column backpressure
Fully porous sub-2-µm particle
columns tend to have ee <0.38
CORTECS solid-core columns tend
to have ee 0.39
A. Daneyko, Anal. Chem. 2011, 83, 3903–3910
Flow Rate (F) = 0.5 mL/min
1.7 µm BEH(Fully Porous)
1.6 µm CORTECS(Solid Core)
©2017 Waters Corporation 15COMPANY CONFIDENTIAL
Similar Backpressure
0
2,000
4,000
6,000
8,000
10,000
12,000
14,000
0.00 0.25 0.50 0.75 1.00 1.25
Pre
ssure
(psi)
Flow Rate (mL/min)
SpecificPermeability
CORTECS UPLC C18+ 1.6 µm: k0 = 2.22E-15 m²
ACQUITY UPLC BEH C18 1.7 µm: k0 = 2.28E-15 m²
Conditions: Tested on an ACQUITY UPLC I-Class using 2.1 x 50 mm columns, 70% Acetonitrile at 30 °C
©2017 Waters Corporation 16COMPANY CONFIDENTIAL
What makes Solid-Core Particles Different from
Fully Porous?
Lets look into the science of how to get lower backpressure and higher
efficiency
– Backpressure equation
– Improved efficiency
o - the van Deemter equation
F. Gritti, G. Guiochon, J. Chromatogr. A 1221 (2012) 2– 40F. Gritti, Chromatography Today May/June (2012) 4-11
©2017 Waters Corporation 17COMPANY CONFIDENTIAL
More Efficient, How???
The van Deemter Equation the basics
The van Deemter equation describes empirically additive sources of
dispersion that result as a function of mobile phase velocity and particle
size.
ucu
baH
0
2
4
6
8
10
0 5 10 15 20 25 30
Linear Velocity u
H-u
©2017 Waters Corporation 18COMPANY CONFIDENTIAL
More Efficient, How???
The van Deemter Equation the basics
The van Deemter equation describes empirically additive sources of
dispersion that result as a function of mobile phase velocity and particle
size.
The a-term was thought to be a constant dp
and takes into account flow heterogeneity
ucu
baH
0
2
4
6
8
10
0 5 10 15 20 25 30
Linear Velocity u
0
2
4
6
8
10
0 5 10 15 20 25 30
Linear Velocity u
H-u
a-term
©2017 Waters Corporation 19COMPANY CONFIDENTIAL
More Efficient, How???
The van Deemter Equation the basics
The van Deemter equation describes empirically additive sources of
dispersion that result as a function of mobile phase velocity and particle
size.
The a-term was thought to be a constant dp
and takes into account flow heterogeneity
The b-term is the longitudinal diffusion term
which diminishes at high linear velocity
ucu
baH
0
2
4
6
8
10
0 5 10 15 20 25 30
Linear Velocity u
0
2
4
6
8
10
0 5 10 15 20 25 30
Linear Velocity u
0
2
4
6
8
10
0 5 10 15 20 25 30
Linear Velocity u
H-u
a-term
b-term
©2017 Waters Corporation 20COMPANY CONFIDENTIAL
More Efficient, How???
The van Deemter Equation the basics
The van Deemter equation describes empirically additive sources of
dispersion that result as a function of mobile phase velocity and particle
size.
The a-term was thought to be a constant dp
and takes into account flow heterogeneity
The b-term is the longitudinal diffusion term
which diminishes at high linear velocity
The c-term is the mass transfer term which
increases at high linear velocity
ucu
baH
0
2
4
6
8
10
0 5 10 15 20 25 30
Linear Velocity u
0
2
4
6
8
10
0 5 10 15 20 25 30
Linear Velocity u
0
2
4
6
8
10
0 5 10 15 20 25 30
Linear Velocity u
0
2
4
6
8
10
0 5 10 15 20 25 30
Linear Velocity u
H-u
a-term
b-term
c-term
©2017 Waters Corporation 21COMPANY CONFIDENTIAL
What makes Solid–Core Particles more Efficient
than Fully Porous???
The one of the major differences for small molecules is in the b-term:
The b-term is the longitudinal diffusion term and is the
easiest to explain and examine:
– At the () optimum linear velocity the b-term
contribution is significant.
– The impervious solid-core at the center of these
particles decrease the volume available for diffusion thereby decreasing the b-
term. Higher Rho-values lead to lower b-terms.
– At higher linear velocities, convective flow dominates and the b-term no longer
plays a significant role.
– Independent measurement of the b-term can be made by peak parking
experiments.
F. Gritti, G. Guiochon, J. Chromatogr. A 1221 (2012) 2– 40K. Miyabe, ANALYTICAL SCIENCES MARCH 2013, VOL. 29
0
2
4
6
8
10
0.0 0.5 1.0 1.5Linear Velocity cm/sec
H-u
©2017 Waters Corporation 22COMPANY CONFIDENTIAL
Reduced van Deemter Equation
h = A+ B/u + Cu
Reduced h for Heptanophenone k' = 3.5
Reduced Linear Velocity (ue)
hmin = 1.9
Solid-Core 1.6 µm CORTECS C18+
A = 0.48, B = 4.20, C = 0.070R² = 0.9994
Fully Porous 1.7 µm BEH C18
A = 0.30, B = 6.93, C = 0.093R² = 0.9997
0
2
4
0 5 10 15 20 25 30
htotal for ACQUITY UPLC 1.7 µm BEH C18
hEddy (A) hLong (B)
hmin = 1.6
htotal for CORTECS UPLC 1.6 µm C18+
0
2
4
0 5 10 15 20 25 30
hmass trans (C) + heat
Comparative separations may not be representative in all applications.
©2017 Waters Corporation 23COMPANY CONFIDENTIAL
Competitive
Solid-Core Comparison
CORTECS 1.6 µm C18 +
Competitor Solid-Core 1.7 µm C18
Solid-Core 1.7 µm Competitor C18
A = 0.85, B = 4.72, C = 0.066R² = 0.9996
3,000
5,000
7,000
9,000
11,000
13,000
15,000
17,000
19,000
21,000
0.0 0.5 1.0 1.5
Pla
tes (
4 s
igm
a)
Flow Rate (mL/min)
-
34%higher
Reduced h for Heptanophenone k' = 3.5 and 3.3
hEddy (A) hLong (B) hmass trans (C) + heat
Solid-Core 1.6 µm CORTECS™ C18+
A = 0.48, B = 4.20, C = 0.070R² = 0.99940
2
4
0 5 10 15 20 25 30
htotal for Competitor Solid-Core 1.7 µm C18
hmin = 1.8
hmin = 1.6
htotal for CORTECS UPLC 1.6 µm C18+
0
2
4
0 5 10 15 20 25 30
Comparative separations may not be representative in all applications.
Reduced Linear Velocity (ue)
©2017 Waters Corporation 24COMPANY CONFIDENTIAL
Competitive
Solid-Core Comparison
CORTECS 1.6 µm C18 +
Competitor Solid-Core 1.7 µm C18
Solid-Core 1.7 µm Competitor C18
A = 0.85, B = 4.72, C = 0.066R² = 0.9996
3,000
5,000
7,000
9,000
11,000
13,000
15,000
17,000
19,000
21,000
0.0 0.5 1.0 1.5
Pla
tes (
4 s
igm
a)
Flow Rate (mL/min)
-
34%higher
Reduced h for Heptanophenone k' = 3.5 and 3.3
hEddy (A) hLong (B) hmass trans (C) + heat
Solid-Core 1.6 µm CORTECS™ C18+
A = 0.48, B = 4.20, C = 0.070R² = 0.99940
2
4
0 5 10 15 20 25 30
htotal for Competitor Solid-Core 1.7 µm C18
hmin = 1.8
hmin = 1.6
htotal for CORTECS UPLC 1.6 µm C18+
0
2
4
0 5 10 15 20 25 30
Comparative separations may not be representative in all applications.
Reduced Linear Velocity (ue)
©2017 Waters Corporation 25COMPANY CONFIDENTIAL
CORTECS Efficiency Advantage
(small molecules)
The largest improvement in efficiency comes from the reduction of the b-
term (longitudinal diffusion)
– The impervious solid core prevents the analytical band from spreading through
the particle
A-term (Eddy dispersion) is a smaller contribution
– Influenced by how well the column bed is packed/arranged
C-term (mass transfer) is already small for small particle columns that
are either fully porous or superficially porous
– The story of a shorter diffusion path is negligible
S. Khirevich et al., J. Chromatogr A 1212 (2010) 4713F. Gritti, G. Guiochon, J. Chromatogr A 1221 (2012) 2– 40
F. Gritti, Chromatogr. Today, May/June (2012), 4-11
©2017 Waters Corporation 26COMPANY CONFIDENTIAL
Agenda
Review of Solid-Core Particles
CORTECS® Solid-Core Particle Columns
©2017 Waters Corporation 27COMPANY CONFIDENTIAL
Agenda
Review of Solid-Core Particles
CORTECS® Solid-Core Particle Columns
©2017 Waters Corporation 28COMPANY CONFIDENTIAL
Complete Confidence
Quality
– Set the industry standard in column
reproducibility
– Experienced primary manufacturer of silica
and hybrid particles
o Own every step of the manufacturing
process
o Exhibit control over our processes
• From synthesis to hardware production
to column packing
High quality, reproducible columns
©2017 Waters Corporation 29COMPANY CONFIDENTIAL
Batch-to-Batch Reproducibility
©2017 Waters Corporation 30COMPANY CONFIDENTIAL
1.00
1.50
2.00
2.50
3.00
3.50
4.00
4.50
5.00
5.50
6.00
0.0 5.0 10.0 15.0 20.0 25.0 30.0
Reduced P
late
Heig
ht,
h (
USP)
Linear Velocity (cm/min)
2.1 x 50 mm
3.0 x 50 mm
4.6 x 50 mm
Consistent packing qualityacross all three diameters
provides for improved scalability and smooth method
transfer
Column Packing Consistency
CORTECS Columns
This is difficult to achieve!
©2017 Waters Corporation 31COMPANY CONFIDENTIAL
Challenges of Efficiently Packing Across
Different Column Diameters
1.00
1.50
2.00
2.50
3.00
3.50
4.00
0.0 5.0 10.0 15.0 20.0 25.0 30.0
Reduce
d P
late
Heig
ht,
h (
USP)
Linear Velocity (cm/min)
Kinetex C18, 2.6µm - van Deemter Performance Across Diameters
2.1 x 50 mm
3.0 x 50 mm
4.6 x 50 mm
Competitive sub-3-µm Solid-Core Columns
Variable efficiencies acrossinternal diameters (14.3% difference in efficiency)
Comparative separations may not be representative in all applications.
E. Oláh, S. Fekete, J. Fekete, K. Ganzler, J Chrom. A 1217, (2010), 3642
2.1 mm
3.0 mm
4.6 mm
©2017 Waters Corporation 32COMPANY CONFIDENTIAL
CORTECS Column Family
UPLC Columns featuring 1.6 µm solid-core silica particles
HPLC/UHPLC Columns featuring 2.7 µm solid-core silica particles
Key Benefits
– High Efficiency
o Resolution
o Speed
– Scalability UPLC HPLC/UHPLC
7 chemistries:
Phenyl
T3
HILIC
Shield RP18
C18+
C18
C8
©2017 Waters Corporation 33COMPANY CONFIDENTIAL
Having Scalability Between Different Solid-Core
Particle Sizes
Solid-Core
Core
Part
icle
Attribute CORTECS
r 0.7
Particle Size 1.6 µm, 2.7 µm
Pore Volume 0.26 cm³/g
Pore Size 90 Å*
Surface Area 100 m²/g
FIB SEM Images
Solid-Core
Core
Part
icle
1.6 µm 2.7 µmRho (ρ) = 0 → fully porous
particle
ρ = 1 → nonporous particle
r = core diameter / particle diameter
Maintaining the rho value,Allows for scalability between the particle sizes
*T3 chemistry is 120 Å
©2017 Waters Corporation 34COMPANY CONFIDENTIAL
Agenda
Review of Solid-Core Particles
CORTECS® Solid-Core Particle Columns
Column Benefits for Improved Laboratory Performance
©2017 Waters Corporation 35COMPANY CONFIDENTIAL
Agenda
Review of Solid-Core Particles
CORTECS® Solid-Core Particle Columns
Column Benefits for Improved Laboratory Performance
©2017 Waters Corporation 36COMPANY CONFIDENTIAL
Performance Benefits
Particle Equivalency
CORTECS Solid-Core Particle Columns
– Efficiencies that are equivalent to that of smaller fully porous particles
– Backpressures equivalent to that of larger fully porous particles
CORTECS Particle Size
Equivalent Porous Particles
Efficiency Backpressure
1.6 µm 1.3 µm 1.8 µm
2.7 µm 2.2 µm 3.1 µm
©2017 Waters Corporation 37COMPANY CONFIDENTIAL
Fully Porous C18, 3.5 µm
Psi: 2900
N peak 4: 17,600
High Efficiency Separation at HPLC
BackpressuresA
U
0.00
0.02
0.04
0.06
0.08
0.10
AU
0.00
0.02
0.04
0.06
0.08
0.10
AU
0.00
0.02
0.04
0.06
0.08
0.10
Minutes
1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50
1
2 34
Rs 4.9
Rs 6.3
Rs 8.2
Fully Porous C18, 5 µm
Psi: 1900
N peak 4: 10,400
CORTECS C18, 2.7 µm
Psi: 3200
N peak 4: 23,600
1. Estradiol2. Ethinyl estradiol3. Estrone4. Levonorgestrel
Configuration: 4.6 x 150 mm
Note: 2.5 µm fully porous Psi ~6000
©2017 Waters Corporation 38COMPANY CONFIDENTIAL
Transferability
Method Transfer
– Scaled synthetic process
o Rho value scaled
– UPLC HPLC
o Seamless method transfer
o Future proofing
1.6 µm UPLC 2.7 µm HPLC/UHPLC
©2017 Waters Corporation 39COMPANY CONFIDENTIAL
Agenda
Review of Solid-Core Particles
CORTECS® Solid-Core Particle Columns
Column Benefits for Improved Laboratory Performance
Practical Applications of CORTECS Columns
– The right column for your challenging assays
©2017 Waters Corporation 40COMPANY CONFIDENTIAL
Agenda
Review of Solid-Core Particles
CORTECS® Solid-Core Particle Columns
Column Benefits for Improved Laboratory Performance
Practical Applications of CORTECS Columns
– The right column for your challenging assays
©2017 Waters Corporation 41COMPANY CONFIDENTIAL
The CORTECS Family
C18
C18+
HILIC
C8
Phenyl
T3
Shield RP18
General Purpose, balanced acidic, basic, neutral analyte retention
Best choice for use with MS detection and low ionic strength acidic mobile phases
Analysis of very polar analytes using HILIC mode
Lower retention; general purpose
Different selectivity particularly for aromatic analytes
Balanced retention of non-polar and polar compounds using RPLC
Different selectivity Improved peak shapes for basic analytes
vs. CORTECS C18
©2017 Waters Corporation 62COMPANY CONFIDENTIAL
Method Transfer:
HPLC UHPLC UPLC
1
2
34 5
AU
0.00
0.01
0.02AU
0.00
0.01
0.02
AU
0.00
0.01
0.02
12.0 14.0 16.0 18.0 20.0 22.0 24.0 26.0 28.0 30.0
3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0
AU
0.00
0.01
0.02
3.5 4.0 4.5 5.0 5.5 6.0 6.0 7.0 7.5 8.0
Minutes
1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6
Fully Porous C18, 5 µm4.6 x 150 mm1.00 mL/minAlliance® HPLC
CORTECS 2.7 µm C184.6 x 75 mm1.85 mL/minAlliance HPLC
CORTECS 2.7 µm C184.6 x 75 mm1.85 mL/minH-Class
CORTECS 1.6 µm C182.1 x 50 mm0.65 mL/minH-Class
Initial Method
Compatible with HPLC, UHPLC, and
UPLC systems
Transfer to 1.6 µm9X Faster method15X Less solvent
Transfer to 2.7 µm4X Faster method2X Less solvent
©2017 Waters Corporation 63COMPANY CONFIDENTIAL
1
2
34 5
AU
0.00
0.01
0.02AU
0.00
0.01
0.02
AU
0.00
0.01
0.02
12.0 14.0 16.0 18.0 20.0 22.0 24.0 26.0 28.0 30.0
3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0
AU
0.00
0.01
0.02
3.5 4.0 4.5 5.0 5.5 6.0 6.0 7.0 7.5 8.0
Minutes
1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6
Fully Porous C18, 5 µm4.6 x 150 mm1.00 mL/minAlliance® HPLC
CORTECS 2.7 µm C184.6 x 75 mm1.85 mL/minAlliance HPLC
CORTECS 2.7 µm C184.6 x 75 mm1.85 mL/minH-Class
CORTECS 1.6 µm C182.1 x 50 mm0.65 mL/minH-Class
Initial Method
Compatible with HPLC, UHPLC, and
UPLC systems
Transfer to 1.6 µm9X Faster method15X Less solvent
Transfer to 2.7 µm4X Faster method2X Less solvent
Method Transfer:
HPLC UHPLC UPLC
©2017 Waters Corporation 64COMPANY CONFIDENTIAL
1
2
34 5
AU
0.00
0.01
0.02AU
0.00
0.01
0.02
AU
0.00
0.01
0.02
12.0 14.0 16.0 18.0 20.0 22.0 24.0 26.0 28.0 30.0
3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0
AU
0.00
0.01
0.02
3.5 4.0 4.5 5.0 5.5 6.0 6.0 7.0 7.5 8.0
Minutes
1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6
Fully Porous C18, 5 µm4.6 x 150 mm1.00 mL/minAlliance® HPLC
CORTECS 2.7 µm C184.6 x 75 mm1.85 mL/minAlliance HPLC
CORTECS 2.7 µm C184.6 x 75 mm1.85 mL/minACQUITY UPLC H-Class
CORTECS 1.6 µm C182.1 x 50 mm0.65 mL/minH-Class
Initial Method
Compatible with UHPLC systems4X Less Solvent
Transfer to 1.6 µm9X Faster method15X Less solvent
Transfer to 2.7 µm4X Faster method2X Less solvent
AU
0.000
0.010
0.020
3.5 4.0 4.5 5.0 5.5 6.0 6.0 7.0 7.5 8.0
CORTECS 2.7 µm C183.0 x 75 mm0.79mL/minACQUITY Arc UHPLC
Method Transfer:
HPLC UHPLC UPLC
©2017 Waters Corporation 65COMPANY CONFIDENTIAL
1
2
34 5
AU
0.00
0.01
0.02AU
0.00
0.01
0.02
AU
0.00
0.01
0.02
12.0 14.0 16.0 18.0 20.0 22.0 24.0 26.0 28.0 30.0
3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0
AU
0.00
0.01
0.02
3.5 4.0 4.5 5.0 5.5 6.0 6.0 7.0 7.5 8.0
Minutes
1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6
Fully Porous C18, 5 µm4.6 x 150 mm1.00 mL/minAlliance® HPLC
CORTECS 2.7 µm C184.6 x 75 mm1.85 mL/minAlliance HPLC
CORTECS 2.7 µm C184.6 x 75 mm1.85 mL/minACQUITY UPLC H-Class
CORTECS 1.6 µm C182.1 x 50 mm0.65 mL/minH-Class
Initial Method
Compatible with UHPLC systems4X Less Solvent
Transfer to 1.6 µm9X Faster method15X Less solvent
Transfer to 2.7 µm4X Faster method2X Less solvent
AU
0.000
0.010
0.020
3.5 4.0 4.5 5.0 5.5 6.0 6.0 7.0 7.5 8.0
CORTECS 2.7 µm C183.0 x 75 mm0.79mL/minACQUITY Arc UHPLC
Method Transfer:
HPLC UHPLC UPLC
©2017 Waters Corporation 66COMPANY CONFIDENTIAL
Agenda
Review of Solid-Core Particles
CORTECS® Solid-Core Particle Columns
Column Benefits for Improved Laboratory Performance
Practical Applications of CORTECS Columns
Summary
©2017 Waters Corporation 67COMPANY CONFIDENTIAL
Summary
CORTECS Solid Core columns set the new gold standard for efficiency,
reproducibility, and quality.
Built on a proven solid-core particle technology, CORTECS Solid-core
Columns provide more information in less time
CORTECS Columns are fully scalable between particle sizes, and can be
used with any UPLC, UHPLC, and HPLC system in your laboratory.
o Ultimate Efficiency = CORTECS 1.6 µm Columns
o Ultimate Utility = CORTECS 2.7 µm Columns
©2017 Waters Corporation 68COMPANY CONFIDENTIAL
Premier Particle Technologies
CORTECS™ Solid-Core Technology
Highestefficiency
Optimized Backpressure
Seamless scalability
BEH Technology
Unparalleled pH stability
Mobile phase and temperature versatility
Seamless scalability
HSS Technology
Enhanced retention
Particle and ligand selectivity
Seamless Scalability
CSH™ Technology
Exceptional loading capacity
Superior basic peak shape
Seamless Scalability
Wide pH Range Wide Selectivity Range High EfficiencyPeak Capacity
©2017 Waters Corporation 69COMPANY CONFIDENTIAL
Thank you for your attention
QUESTIONS
©2017 Waters Corporation 71COMPANY CONFIDENTIAL
CORTECS Performance, when System and Column
Dispersion are not Matched
UHPLCDispersion 30 – 12 µL
UPLCDispersion <12 µL
HPLCDispersion >30 µL
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00 2.50
2.1 mm ID1.6 μm50mm length
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00 2.50
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00 2.50
3.0 mm ID2.7 μm50mm length
4.6 mm ID2.7 μm50mm length
©2017 Waters Corporation 72COMPANY CONFIDENTIAL
CORTECS Performance, when System and Column
Dispersion are not Matched
UHPLC UPLCHPLC
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00 2.50
2.1 mm ID1.6 μm50mm length
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00 2.50
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00
AU
0.00
0.20
0.40
0.60
0.80
1.00
Minutes
0.50 1.00 1.50 2.00 2.50
3.0 mm ID2.7 μm50mm length
4.6 mm ID2.7 μm50mm length