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ISO 13485 U U n n c c o o n n j j u u g g a a t t e e d d E E s s t t r r i i o o l l R R I I A A Code: RK-3CT 100 tubes Radioimmunoassay for the quantitative in vitro determination of unconjugated Estriol (uE3) in human serum. ESSENTIALS • Simple format, coated tube assay • One step procedure, no washing THEORY IN PICTURES FLOW CHART Add to coated tubes: • 50 µL calibrator/control/sample • 500 µL tracer • 500 µL antiserum 2 h incubation at RT with shaking Competition between unlabelled uE3 and 125 I-labelled uE3 for a limited number of binding sites on uE3 specific antibody.
