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    UNIT TWO - UNITY OF LIFE

    CBSE XI - BIOLOGY

    CHAPTER - 4 THE CELL AS A UNIT OF LIFE

    Important points

    1) According to the cell Theory, all living organisms are made of one or

    more cells.

    Cells are the basic units of living organisms. All cells arise from pre-

    existing cells.

    2) Cells of all organisms have close similarities in structure, molecular

    organisation and biological activities. These emphasise unity of life.

    3) Each cell functions as a Self-contained unit with considerable

    autonomy. It can independently carry out nutrition, respiration,

    growth, reproduction and self-regulation.

    4) In a multicellular organism, cells are not totally independent on

    one another. Some of their functions may not be operative. However,

    isolated living cells of multicellular organisms can be cultured for

    many generations in nutrient media. Thus the ability for independent

    existence is not lost by a cell even if it forms part of a specialised

    tissues in a multicellular body.

    5) In a unicellular organism the single cell has to perform all its life

    processes. The presence of specialised structures called organelles

    helps the cell to carry out and regulate specific activities. In

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    multicellular organisms distinct types of cells differentiate and

    perform different activities. The cells co-operate among themselves

    and participate in common functions.

    6) Cells obtain a constant flow of energy through the process of

    photosynthesis from the chemical bond energy of food.

    7) Cells regulate their life processes with the help of continual flow of

    information.

    This may be genetic information carried by DNA or information

    coming from outside.

    8) Compartmentalisation is essential for cellular life. The cell with its

    plasma membrane acts as a compartment. In plant cells the plasma

    membrane is surrounded by a cellulose wall. Eukaryotic cells

    possess many compartments, the membrane-bound organelles.

    Each organelle has a specific structure and function. Procaryotic

    cells do not show any intracellular compartments.

    Definitions

    1. Basal body-Centriole like structures near the cell membrane from

    which cilia or flagella originate.

    2. Cell-The basic unit of a plant or animal. It is an individual, usually

    microscopic mass of living protoplasm.

    3. Cell theory - The concept which states that all living things/organisms

    are made up of cells and that it is by their division that process

    such as growth and reproduction takes place.

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    4. Cell-wall-The non-living external layer of a cell in plants. It is made

    up of cellulose.

    5. Cytoplasm - A part of protoplasm within the cell bounded by cell

    wall and containing nucleus and cell organelles.

    6. Cellular totipotency - The ability of somatic cell of a plant to produce

    a complete plant.

    7. Centriole - A minute granular self-replicating body from which astral

    rays and spindle fibres arise forming a spindle during cell division.

    8. Centrosome - A small clear cytoplasmic body containing centriole,

    situated just outside the nuclear membrane in animal cell and helps

    in spindle formation.

    9. Chromosome - Deeply staining thread-like structure within the

    nucleus of a cell which carries hereditary information.

    10. Chromoplast - coloured plastid containing pigments other than

    chlorophyll.

    11. Chlorophyll - Green pigment of plants which is meant for capturing

    solar energy of sun for photosynthesis.

    12. Cellulose - A complex Carbohydrate which consists of a chain of

    glucose molecules, present in the cell wall of all plants except fungi.

    13. Cilia - Minute, hair like protoplasmic processes arising from the

    cell surface.

    14. Cell Sap - The liquid content present in the vacuole of plant cell.

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    15. Dictyosome - A plant cell organelle consisting of stacks of

    membranous sacs and vesicles that enclose and store cell products.

    16. Endoplasmic Recticulum - A system of Channels and tubes in the

    cytoplasm which functions as site of protein Synthesis and transport.

    17. Entropy - The phenomenon in which all physical and chemical

    changes tend to proceed in such a direction that useful energy

    undergoes irreversible degradation into random form called entropy.

    18. Lamella - A thin ribbon like structure present in Chloroplast of plant

    cells.

    19. Lysosome - Single membranous bag - like organelle containing

    digestive enzymes, often referred to as suicidal bags.

    20. Micro-filament - A very thin, thread - like structure made of protein

    which occurs in the cytoplasm of most cells.

    21. Nucleoplasm-The fluid part of nucleus is called nucleoplasm.

    22. Organelle - Any part of the cell that has a specilised function.

    23. Plastid - A membrane bounded body in the cytoplasm which

    contains DNA, pigments and food reserved. They are found only in

    plant cells.

    24. Tonoplast - The inner plasma membrane of a cell in plants which

    separates the vacuoles from the cytoplasm.

    25. Vesicle - A thin walled drop like structure or a cavity containing

    fluid.

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    Very Short Answers

    Q.1. Define cell.

    A. The cell is the structural and functional unit of life.

    Q.2. Name the following.

    a) Scientist who discovered the cell.

    A. Robert Hooke.

    b) Scientist who discovered Nucleus and cytoplasm.

    A. Nucleus - Robert Brown ,cytoplasm - Dijardin.

    c) Who proposed the cell Theory.

    A. M.J. Schleiden and T. Schwann.

    d) Who introduced the idea of Omnis-Cellula-e-Cellula.

    A. Rudolf Virchow.

    e) Who introduced the Crude microscope.

    A. Antony - Von - Leewenhoek.

    f) Name the smallest cell.

    A. PPLO (Pleuro - Pneumonia like organism).

    g) Name the book published by Robert Hooke.

    A. Micrographia.

    h) What is the size of smallest virus ?

    A. It is 7.0 x 10-7.

    i) What is the size of a unicellular eucaryote ?

    1-2mm to 1mm

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    Q.3. A group of cells with similar structures and performing same

    functions constitute a tissue.

    Q.4. Nucleus was discovered by Robert Brown in cells of Roots of orchids.

    Q.5. A large cell has proportionately Smaller surface area.

    Q.6. The cell derives energy from its surroundings continually to reduce

    entropy.

    Q.7. Animals and heterotrophic plants derive energy in the form of bond

    energy from Food.

    Q.8. Extrinsic flow of information takes place in the form of informational

    molecules.

    Q.9. A human erythrocyte is 7 - 8 um in diameter.

    Q.10. A striated muscle cell is 15 - 20mm long and 30 to 40um in diameter.

    Q.11.The membrane around the vacuole is called tonoplast.

    Q.12.Who concluded, Cells are the ultimate units forming structure of

    all plant tissues ?

    A. M.J. Schleiden.

    Q.13.Define cell culture ?

    A. Cell culture is the technique of growing isolated cells in a sterile

    nutrient medium under controlled conditions.

    Q.14.hat is meant by cell differentiation ?

    A. Cell differentiation is the process of specialisation in a structure to

    assume a specific function.

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    Q.15.What is totipotency ?

    A. Capacity of every living plant cell to regenerate a whole plant is

    called totipotency.

    Q.16.What are the 2 forms in which energy is derived by living beings ?

    A. Light energy & chemical energy.

    Q.17.In what form is the information transferred within the cell ?

    A. The information is transferred in the form of informational molecules

    of nucleic acids within the cells.

    Q.18.What are informational molecules ?

    A. Informational molecules are macromolecules having a specific

    sequence.

    Q.19.What are receptor molecules.

    A. Receptor molecules are specific proteins in the cell membrane that

    enter into the cells like hormones.

    Q.20.Name 2 cell organalle which are bounded by a single membrane.

    A. Vacuole & Lysosome.

    Q.21.Which cell organelle maintains the internal envt.

    of the cell ?

    A. Vacuoles.

    Q.22.Name any 2 double membraned cell organelle.

    A. Mitochondria and nucleus.

    Q.23.Which mature body cells are incapable of reproduction ?

    A. Nerve cells.

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    Q.24.Who gave the term Protoplasm ?

    A. Purkinjie.

    Q.25.Who proposed Omnis cellula-e-cellula ?

    A. Rudolf Virchow (all cells arise from pre existing cells).

    Q.26,Define (a) cellus (b) Heterokaryon.

    A. (a) Mass of unorganized cells developed from single isolated cells

    in nutrient medium is called Callus.

    (b) The fusion product of protoplasm of 2 -somatic cells is called

    heterokeryon.

    Short-Answers Questions

    Q.1. How did Robert Hooke describe the cell when he saw it for the first

    time ?

    A. Robert Hooke described the cell as box like compartments in the

    body of a plant, which acted as passages for conduting fluids. He

    considered them as cell walls enclosing an empty space.

    Q.2. What are the fundamental similarities in all cells ?

    A. Fundamental similarities in all cells are -

    i) Hereditary characters are transmitted in the form of nucleic acids.

    ii) Basic structure of membranes and their properties.

    iii) Mechanism of aerobic respiration.

    iv) Mechanism of synthesis of nucleic acids and proteins.

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    Q.3. Why do you say cell is a Self contained Unit ?

    A. Every cell acts as a self contained unit or autonomous unit. All

    fundamental biological processes are performed by a cell

    independently. Food is oxidised to produce energy in the cell.

    It synthesizes complex molecules and uses these molecules to

    make new structure and replace worn out ones. The cell reproduces,

    regulates all activities and maintains the necessary internal physico

    - chemical conditions. They multiply and produce a large population

    of cells or a tissue to perform all vital activities.

    Q.4. What are the main steps of plant tissue culture technique ?

    The main steps are -

    i) Isolation of cells from multicellular organism in aspetic conditions.

    ii) Transfer of the cells to a sterile nutrient medium under aseptic

    conditions.

    iii) Maintenance of suitable PH, nutrients, vitamins and hormone etc.

    Q.5. What are the major applications of the tissue culture technique ?

    A. i) To develop disease free plants.

    ii) Rapid multiplication of desirable characters of plants.

    iii) To produce large number of haploid plants.

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    iv) To get somatiz hybrids (heterokaryon).

    v) to produce homozygons diploids.

    vi) To control fert il ization, parthenocarpy, genetical plant

    hybridization etc.

    Q.6. What are the advantages of multicellularity.

    A. The Advantages of multicellularity are as follows -

    a) Divison of Labour is the main aspect of multicellularity.

    b) Tissue formation takes place due to division of labour.

    c) Organs and organ systems are formed by tissue which work in

    co-ordination among themselves for the benefit of the organism.

    d) Multicellularity provides differentiation in structure and

    function.

    Q.7. Explain why the cells of unicellular organism are generally spherical,

    whereas those of multicellular may be of many shapes.

    A. The shape of the cell depends upon -

    1) Age of cell 2) Viscosity of cell sap

    3) External pressure 4) Internal skeleton

    5) Function of cell 6) Surface tension (it shapes the cell

    to be spherical).

    Free floating cells having thin membrane may be spherical. When

    the cells multiply (because of multicellular) the shape of the cells

    changes in accordance to the available space.

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    Q.8. What is cell theory ? who proposed it ?

    A. Modern concept of cell theory.

    1) Origin, nature and continuity of life is bounded in the cell.

    2) The growth of the organism is due to growth of cell or cells

    products.

    3) Life is passed from one generation to another through a single

    cell.

    4) The function of an organism as a whole is the outcome of the

    activities and interactions of the constituent cells.

    5) All cells are basically alike in chemical composition and

    interactions of constituent cells.

    6) Omnis cellula-e-cellula i.e. all cells arise from pre-existing

    cells.

    The cell theory was put forward by M.J. Schleiden and T.

    Schwann.

    Q.9. No cell can have a volume whose metabolic requirement exceed

    the exchange capabilities of its surface. Comment.

    A. Normally a cell which is metabolically very active cannot have a

    very large volume. Cell volume depends on the exchange capabilities

    of its surface. Small bodies have more surface per unit volume than

    large bodies. Hence, no cell can have a very large volume whose

    metabolic requirement exceed the exchange capabilities of its

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    2 cm2 cm

    1 cm 3 cm 3 cm

    Surface Volume ratio

    Q.10. Plant cells are totipotent - Comment.

    Steward and his co-workers stated the above statement. They

    conducted their experitent on mature Carrot roots cells. They grew

    these cells in a suitable culture medium supplemented with

    coconut milk.

    They showed that the isolated cells formed an undifferentiated mass

    of cells called Callus which differentiated into plantlets. Thus the

    ability of mature excised cells to give rise to full individual in a

    nutrient medium is known as Cellular totipotency .

    DIAGRAM GIVEN BELOW

    STEWARDS EXPERIMENT DEMONSTRATING CELLULAR

    TOTIPOTENCY IN CARROT.

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    Storage

    root

    T.S. of the

    root

    2 mgphloem

    plants

    Plants cultured in

    medium + coconut milk

    Portion of culture

    Embryoid

    from culture

    free cells

    Cells from

    Embryo

    Plantlet

    Flowering

    plant

    Flow

    er

    Seeds

    Phloe

    m

    explan

    ts

    Cellfrom

    Phloem

    Free cells in

    suspension

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    Q.11. Do the dead cells play any role in a living organism ?

    A. These dead cells are of immense value for the plants as well as for

    animals. The xylem vessels and phloem fibres provide strength to

    the plant and help to increase the bulk of plant. The dead cells act

    as protective covering.

    Q.12.How can you say that multicellular organism has greater capacity

    for survival than unicellular organism?

    A. In a multicellular organism, a number of cells are constantly lost

    from the outer surface of skin and these are replaced by the new

    cells formed by the division of cells in the lower layers. The red

    blood cells have a span of 100-120 days. These are regularly replaced

    by the new cells formed in the bone narrow of long bones. On the

    other, any damage to a single-celled organism may easily cause its

    death.

    Q.13.Distinguish between the following

    a) Extrinsic flow of information

    i) Flow of genetic information

    within the cell.

    ii) Regulates all activities of the

    cell.

    iii) Information flows in the form

    of nucleic acids.

    Intrinsic flow of information

    i) Flow of functional information

    from outside the cell.

    ii) Regulates some activities of

    the cell.

    iii) Information flows in the form

    of protein or other types of

    moleculs.

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    b) Procaryotic cell

    i) Cell wall present non

    cellulosic, composed of

    amino sugars and muramic

    acids.

    ii) Plasma membrane simple.

    iii) Chlorophyll, whenever

    present is not in Chloroplast.

    iv] Membrane bound

    organelles like endo plasmic

    reticulum golgi complex,

    mitochoridria, lysosomes.

    etc. absent.

    v] Nuclear membrane and

    necleolus absent.

    vi] Single chromosome with

    DNA as hereditary material.

    vii] Mitotic apparatus abs. cell

    division by amitosis.

    Eucaryotic cell

    i) Cell wall, when present, is of

    cellulosic plant cells.

    ii) Plasma membrane

    complicated and

    differentiated for different

    functions.

    iii) Chlorophyll when present is

    always in chloroplast.

    iv] Membrane bound organelles

    (endo membranes) present.

    v] These are present.

    vi] More than one chromosome

    DNA is hereditary material.

    vii] It is pr. cell division by mitosis,

    meiosis or amitosis.

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    viii] Flagella lack 9+2 pattern.

    ix] Respiratory enzymes are

    located in the plasma

    membrane.

    viii] Flagella, whenever, pr, possess

    9+2 pattern.

    ix] These are present in pattern

    mitochondria.

    Q.14. What is the chemical nature of the plasma membrane ? Also mention

    the function of plasma membrane ?

    A. The plasma membrane is trilaminar structure. The outer two

    membrane are formed of proteins. The protein layers cover the bimolecular

    phospholipid layer in the centre. It is living and semi-permeable in nature.

    Function:-

    1] Forms the outer covering of the cell. Thus it protects the internal

    cell organelles.

    2] It controls the entry and exit of substances in the cell and thus it

    maintains the chemical nature of the cell.

    Q.15. Diagrammatically represent the flow of energy in a cell ?

    Chloroplasts

    Photosynthesis

    Nutrient molecules

    Oxidation

    ATP synthesis

    Light Photos

    Food

    Molecules

    Flow of energy

    into cell

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    Q.16. Diagrammatically represent the flow of information into a cell ?

    Long answers.( 80 to 150 words )

    1] On what basis can we consider cell as an autonomous unit ?

    A. The cell be considered as an autonomous unit because.

    i] Each cell carries out all fundamental biological processes

    independently.

    ii] Each cell oxidises food material and utilises that energy and some

    nutrient molecules to synthesize complex molecule.

    iii] The cell uses these moleules to build new structures and to replace

    worn out tissue.

    iv] The cell respires and exchanges gases with the envt.

    v] It reproduces to form new cells with similar hereditary characters.

    vi] It also maintains internal physico-chemical conditions.

    Informational

    molecules

    Cellulareffects

    Cellularreceptor

    ProteinRNA

    DNA

    Membranereceptor

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    Q.2. Discuses how the method of science is reflected in the formulation

    of the cell theory.

    A. The following steps of scientific method are reflected in formation of

    cell theory.

    1] Observation. 2] Making a hypothesis

    3] Testing or experimentation and theory.

    1] Robert hooke in 1667 discovered cell Thus the fact that cork was

    made of cells observed by him.

    2] The fact was confirmed by Dutrochet that plants were made up of

    cells.

    3] Robert Brown in 1831 discovered nucleus.

    4] M.J. Schleiden in 1838 after examining a variety of plant tissues

    concluded that the cells were ultimate units forming structure of all plant

    tissues.

    5] T.Schwann in 1939 after observing many types of animal cell defined

    the cell as a membrane bound. nucleus containing structure. He

    proposed the cell hypothesis.

    6] From the obervations of schwann and schleiden and others, a new

    theory was born. Thus, a hypothesis was confirmed by years of verification

    forming a theory.

    7] The theory was modified by virchow in 1855. He gave the hypothesis

    that all cells come from pre-existing cells. This illustrates that the theories

    can change when new discoveries are made.

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    Q.3. If, as the second law of thermodynamies states, The free energy in

    any system tends to decrease, how is it that the earth maintains so many

    living organisums, each in a highly organised, high free energy state ?

    A.3. The earth maintains its organisation in the following way.

    a] To maintain the organisation, any system requires a continous in

    flow of energy to reduce entropy.

    b] Earth receives a continous supply of energy from the sun in the

    from of photons of ligth.

    c] 0.2 to 1% of the solar energy received by the earth enters the

    biosphere in the form of chemical energy through the process of

    photosynthesis.

    d] Flow of energy takes place from photosynthesisers to heterotrophs

    forming food chains.

    e] Approximately 10% of the energy is conserved at each trophic level.

    Thus, all living organisms at each level derive energy to maintian its

    organisation and high free energy state.

    Q.4. What is meant by Flow of information? What is the difference

    between extrinsic and intrinsic flow of information?

    DIAGRAM

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    All the living cells depend on the flow of information.

    The latter adopts two different types of routes.

    i] The flow of genetic information:- The genetic information is given by

    DNA. DNA directs the synthesis of RNA to control the various metabolic

    activities of the cell and ultimately of the organism as a whole. The

    messenger RNA convey the message of DNA to the cytoplasm. The

    m-RNA molecules carry complete information for the synthesis fo more

    than one protein molecules. The kinds of proteins formed are determined

    by the molecular structure of DNA. These proteins are enzyme regulators

    which activates all the vital activities of the cell.

    ii] The flow of extrinsic information. Molecules like hormones pass

    information, coming from outside to the cell. Some of these enter the cell

    to influence its activities. Others bind on to the cell surface with certain

    molecules that serve as receptors such binding leads to significant charges

    in cellular activities.

    Q.5. Justify the statement Cell is the basic unit of life ?

    A. Each cell is an autonomous unit. It perform all fundamental vital

    activities of life. It has mitochondria for the oxidation of food and to produce

    energy. It utilises nutrient molecules to synthesise complex moleules. It

    has the power of division to increase its number for growth and heal up

    the wounds. The cell respires and exchange gases with its surroundings.

    It reproduces cells with similar genetic traits. It can regulate its activities

    to maintian its phyico-chemical environment.

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