VCE VET LABORATORY SKILLSWritten examination
Wednesday 16 November 2016 Reading time: 9.00 am to 9.15 am (15 minutes) Writing time: 9.15 am to 10.45 am (1 hour 30 minutes)
QUESTION AND ANSWER BOOK
Structure of bookSection Number of
questionsNumber of questions
to be answeredNumber of
marks
A 20 20 20B 17 17 80
Total 100
• Studentsarepermittedtobringintotheexaminationroom:pens,pencils,highlighters,erasers,sharpeners,rulersandonescientificcalculator.
• StudentsareNOTpermittedtobringintotheexaminationroom:blanksheetsofpaperand/orcorrectionfluid/tape.
Materials supplied• Questionandanswerbookof19pages.• Answersheetformultiple-choicequestions.
Instructions• Writeyourstudent numberinthespaceprovidedaboveonthispage.• Checkthatyournameandstudent numberasprintedonyouranswersheetformultiple-choice
questionsarecorrect,andsignyournameinthespaceprovidedtoverifythis.• AllwrittenresponsesmustbeinEnglish.
At the end of the examination• Placetheanswersheetformultiple-choicequestionsinsidethefrontcoverofthisbook.
Students are NOT permitted to bring mobile phones and/or any other unauthorised electronic devices into the examination room.
©VICTORIANCURRICULUMANDASSESSMENTAUTHORITY2016
SUPERVISOR TO ATTACH PROCESSING LABEL HEREVictorian Certificate of Education 2016
STUDENT NUMBER
Letter
2016VETLABSKILLSEXAM 2
Question 1Whilealaboratorytechnicianischeckingsomemicroscopes,shenoticesthetagsontheelectricalleadsareoutofdate.Whatshouldshedo?A. Informthelaboratorysupervisor.B. Replacethemicroscopeswithnewones.C. Testtheelectricalleadsherselfwithamultimeter.D. Cleantheelectricalleadswithethanoltoremoveanyoil.
Question 2Whichoneofthefollowingpiecesofequipmentinalaboratorydoesnotregularlyrequireacalibrationoftemperaturecheck?A. dryingovenB. microwaveovenC. refrigerationunitsD. autoclaveforsterilising
Question 3Whenperforminganasepticsamplingtechniqueonadairyfoodproductionline,asidefromalaboratorycoat,whatwouldbethemostappropriateprotectiveclothingtouse?A. acapforthehair,closedfootwearandsafetyglassesB. acapforthehair,gloves,closedfootwearandsafetyglassesC. latexgloves,afacemask,acapforthehairandsafetyglassesD. afacemask,acapforthehair,closedfootwear,glovesandsafetyglasses
Question 4Toprepareasolutionofapproximately0.1Msodiumhydroxide(NaOH),therelevantstandardmethodistoweigh2.00gofNaOHpellets,thenA. placea500mLstandardflaskinanicebath,add200mLofdistilledwaterandtheNaOHpellets,
agitatetodissolve,andmakeuptothemarkwithdistilledwater.B. add200mLofdistilledwatertoa500mLstandardflask,addtheNaOHpellets,dissolveandthenmake
uptothemarkwithdistilledwater.C. placea500mLstandardflaskinanicebath,addtheNaOHpelletstotheflask,thenadddistilledwater
andmakeuptothemark.D. placetheNaOHpelletsina500mLstandardflask,dissolveindistilledwaterandthenmakeuptothe
mark.
SECTION A – Multiple-choice questions
Instructions for Section AAnswerall questionsinpencilontheanswersheetprovidedformultiple-choicequestions.Choosetheresponsethatiscorrect forthequestion.Acorrectanswerscores1;anincorrectanswerscores0.Markswillnotbedeductedforincorrectanswers.Nomarkswillbegivenifmorethanoneansweriscompletedforanyquestion.
SECTION A – continued
3 2016VETLABSKILLSEXAM
Question 5ThebestwaytocleananddisinfectaninoculatingloopbeforereturningittotheequipmentstoreistoA. holdtheloopintheblueflameofaBunsenburneruntilitturnsorange.B. holdtheloopintheyellowpartofaBunsenburnerflameforthreeseconds.C. soaktheloopindetergentandwaterbeforeallowingittodrynaturally.D. washtheloopindetergentandwater,anddryitwithapapertowel.
Question 6WhatdoesSOPstandfor?A. safeoperatingprotocolB. safeoperatingprocedureC. standardoperatingprotocolD. standardoperatingprocedures
Question 7Whenperforminganasepticsamplingtechniqueonafoodsample,themostappropriateequipmenttousewouldbealaminarflowcabinetaswellasA. aBunsenburner,aninoculantloop,sterileglasswareandagrowingmedium.B. ethanol,aninoculantloop,sterilecleanglasswareandagrowingmedium.C. aBunsenburner,aninoculantloop,glasswareandagrowingmedium.D. aBunsenburner,aninoculantloopandsterilecleanglassware.
Question 8ItisnecessarytokeeptheworkingareanearamicroscopecleaninordertoA. createapleasantworkenvironment.B. preventcontaminationbyoilordust.C. stopamessbeingmade.D. helporganisethearea.
Question 9Whichofthefollowingorganellesareincludedinabasicanimalcell?A. cellmembrane,chloroplastandmitochondriaB. cellmembrane,nucleusandmitochondriaC. largevacuole,nucleusandmitochondriaD. cellwall,nucleusandmitochondria
Question 10Whichofthefollowingwouldbeleast likelytobeincludedinanemployee’scompanyhandbookforservicingcustomerrequirements?A. makingtestresultsreadilyavailablewhenrequested,beforebeingapprovedbyasupervisorB. followingenterprisepolicyandproceduresC. workingdiligentlyandresponsiblyD. behavinghonestlyandopenly
SECTION A – continuedTURN OVER
2016VETLABSKILLSEXAM 4
Question 11Sterilisedsalinesolutionsareusedasanemergencyeyewashinalaboratory.Asterilisedsalinesolutionismadebyadding9.5gofsodiumchloride,NaCl(s),towaterandthenmakingthevolumeupto100mLwithwater.Inadditiontothechemicalname‘sodiumchloride,NaCl(aq)’,whichoneofthefollowingconcentrationswillthelabeldisplay?A. 0.95%m/vB. 9.5%v/vC. 9.5%m/vD. 95%m/v
Question 12Whatisthepurposeofswabbingaworksurfaceimmediatelyaftercleaningwithethanol?A. tomaintainsterilityB. topreventcross-infectionC. topreventcross-contaminationD. tocheckformicrobiologicalgrowth
Question 13Whatisthemostappropriatewaytocleanupamicroscopeimmersionoilspillonalaboratorybench?A. Wetdownwithwaterandwipeupthespillwithapapertowel.B. Putanacidsolutionontheoilandwipeupthespillwithapapertowel.C. Putastrongbasesolutionontheoilandwipeupthespillwithapapertowel.D. Wetdownwithadetergentsolutionandthenwipeupthespillwithapapertowel.
Question 14Pyrexglasswarewouldnotbesuitableforwhichoneofthefollowingprocedures?A. forstoringculturemediainacupboardB. whenheatingsolutionsaboveaBunsenburnerC. forstoringstrongcausticsolutionsinacupboardD. whenheatingsolutionsbyplacingthemstraightontoahotplate
Question 15SustainableenergyworkpracticeswouldnotincludeA. examiningworkpracticesthatuseexcessiveelectricity.B. liftingmanuallyinsteadofusingmachines.C. switchingoffequipmentwhennotinuse.D. minimisingprocesswaste.
Question 16Thesafestandmostappropriatemethodofhandlingcoolglassware,includingflasks,bottlesandbeakers,istoholditA. bythesidesonly.B. bythesidesandthebottomonlyandnotbythetoportherim.C. atthetoparoundtherim,whileusingtheotherhandtosupportthebottom.D. atthetoparoundtherimofabeakerandaroundtheneckofabottleandflask.
SECTION A – continued
5 2016VETLABSKILLSEXAM
Question 17Alaboratorytechnicianhasthetaskofcleaningoutthechemicalstoreroom.Duringthistask,hefindsasmall,sealedbottle.Thelabelonthebottleishardtoreadandthetechniciancanunderstandonlyasmallpartofit.Whatshouldhedo?A. Placethebottleinthechemicalrubbishbin.B. Tipthecontentsdownthesinksothebottlecanbere-used.C. Informthelaboratorysupervisorandplacethebottleinadesignatedareaforsafedisposallater.D. Obtainanewlabel,includetheinformationthatcanbeunderstoodandmakealogicalguessaboutthe
restoftheinformation.
Question 18Ethanolsolutionisveryusefulinthelaboratoryasasolventandforcleaningsurfaces.Asolutionofethanolhasaconcentrationof10%v/v,butaworkingsolutionof2%v/visrequired.Howmuchwaterneedstobeaddedto50mLofthe10%v/vtomaketheconcentration2%v/v?A. 5mLB. 50mLC. 200mLD. 250mL
Question 19Aclientcallsandasksatechniciantochangesometestresultsastheclientbelievesthemtobeincorrect.Oncheckingthetestresults,thetechniciandiscoversatransfererror.Whatactionshouldthetechniciantake?A. Changethetestresultsandreissuethereportaftercheckingwiththelaboratorymanager.B. Changethetestresultsandreissuethereportbeforecheckingwiththelaboratorymanager.C. Changethetestresultsandreissuethereportaftercheckingwithacolleague.D. Changethetestresults,reissuethereportandapologisetotheclient.
Question 20Whenusingglassware,whichoneofthefollowingfactorsaffectstheaccuracyoftheresults?A. achipontherimofabeakerB. ahairlinecrackonthesideofabeakerC. atextamarkontheoutsideofabeakerD. alabelplacedontheoutsideofabeaker
END OF SECTION ATURN OVER
2016VETLABSKILLSEXAM 6
Question 1 (6marks)Identifythehazardwarningindicatedforeachofthesafetysignsbelow.Listtheassociatedpersonalprotectiveequipment(PPE)thatwouldberequiredwhenworkingwithsolutionsandmaterialsinvolvingeachhazardinalaboratorysetting.
1.
Hazardwarning
PPErequired
2.
Hazardwarning
PPErequired
3.
Hazardwarning
PPErequired
SECTION B – Short-answer questions
Instructions for Section BAnswerall questionsinthespacesprovided.
SECTION B – continued
7 2016VETLABSKILLSEXAM
Question 2 (3marks)Listthreegoodpracticesthatsupportasustainableenergyapproachintheworkplace.
1.
2.
3.
SECTION B – continuedTURN OVER
2016VETLABSKILLSEXAM 8
Question 3 (7marks)AtechnicianworkinginapathologylaboratoryhasbeenaskedtocarryoutaGramstaintestonasamplesuspectedtobeSalmonella enterica.Theresultsseenontheslideareshownbelow.Theresultsappearpinkincolour.
a. Describethetestresultsthatwouldberecordedforthisslide. 1mark
b. Givetwo reasonsthatexplainthetestresultsdescribedinpart a. 2marks
c. TherearetwocoloursthatindicatetheresultsofaGramstaintest.Oneoftheresultsisindicatedbythecolourpink.
Identifythecolouroftheotherresult. 1mark
d. Explainhowthetestcausesthedifferencesincolour. 3marks
SECTION B – continued
9 2016VETLABSKILLSEXAM
Question 4 (6marks)a. Explainthedifferencebetweencross-infectionandcross-contamination. 2marks
b. Severalsampleswereacceptedbyalaboratoryformicrobiologicaltesting.
Givetwotechniquesthatshouldbeusedtotransferthesamplesasepticallyinordertopreventcross-contamination.Provideanexampleforeachtechnique. 4marks
Technique1
Example
Technique2
Example
Question 5 (3marks)a. Givetworeasonsforalaboratorytechniciantohaveanunderstandingofthebusinessgoals,
productsandservicesofthecompanytheyworkfor. 2marks
1.
2.
b. Giveoneexampleofhowalaboratorytechnician’ssuggestionstoimproveproductsandservicescouldassistinsupportingthecompany’sgoals. 1mark
SECTION B – continuedTURN OVER
2016VETLABSKILLSEXAM 10
Question 6 (5marks)Alaboratorytechnicianhasbeenaskedtoexamineaslideunderamicroscope.Animageoftheslideisshownbelow.Theslideshowsasampleofanimalcells.
a. Twoofthesecellslookverydifferentfromtheotherfive.
Giveanexplanationofwhatmightbehappeningtothesetwocells. 2marks
b. Whatisthebiologicaltermforthisprocess? 1mark
c. Hasthisslidebeencontaminated?Giveonereasonforyouranswer. 2marks
SECTION B – continued
11 2016VETLABSKILLSEXAM
Question 7 (2marks)Thediagrambelowshowsaclose-upofasectionofthesideofa100mLmeasuringcylinder.Thescaleshownonthesideofthemeasuringcylinderislabelledinincrementsof10mL,withsmallerincrementsinbetween.
60
50
40
a. Atechnicianusesameasuringcylindertomeasure50mLofasolution.
Onthemeasuringcylinderabove,indicatethepositionofthemeniscusthatwouldgivethemostaccuratereadingfor50mLofasolution. 1mark
b. Anothertechnicianinthesamelaboratoryhasassistedinmeasuring50mLofthesamesolutionineachoffourothermeasuringcylinders.Thesehavebeenleftonthebench,readytobeusedinalaboratoryprocedure.
Thetechnicianchecksthesemeasuresbeforecontinuingwiththeprocedureandfindsthatoneofthecylindershas52mLofthesolution.
Whatshouldthetechniciandonext? 1mark
SECTION B – continuedTURN OVER
2016VETLABSKILLSEXAM 12
Question 8 (6marks)Atypicallightmicroscopeisshownbelow.
a. Labelthefollowingpartsofthelightmicroscopeonthediagramabove:• stage• eyepiecelens• objectivelens• condenser 4marks
b. Whatisthepurposeofeachofthefollowingpartsofthelightmicroscope? 2marks
• Objectivelens
• Condenser
SECTION B – continued
13 2016VETLABSKILLSEXAM
Question 9 (2marks)Describehowtheenvironmentalimpactofwastegeneratedinalaboratoryorproductionlinecouldbeminimisedintheworkplace.Givetwoexamplestosupportyouranswer.
Question 10 (5marks)Thefollowingquestionsrefertomakingaprimarystandardsolutionofsodiumcarbonate,Na2CO3.
a. GivetworeasonswhyNa2CO3(s)isanexcellentprimarystandard. 2marks
b. AspartoftheprocedurewhenmakingaprimaryNa2CO3standardsolution,thechemicalisdried.
i. WhyistheNa2CO3dried? 1mark
ii. WhatistheoptimumtemperatureatwhichtodryNa2CO3(s)? 1mark
iii. Afterdrying,howwouldtheNa2CO3(s)bestoredtokeepitdry? 1mark
SECTION B – continuedTURN OVER
2016VETLABSKILLSEXAM 14
Question 11 (4marks)Theresultsontheagarplatesbelowindicatethatfouroutof10platesarecontaminated.
a. Identify twopossiblecausesofcontamination. 2marks
1.
2.
b. Apartfromswabbingtheworksurface,suggesttwowaysofapplyinglaboratorypracticesthatwouldassistineliminatingthecausesofcontaminationidentifiedinpart a. 2marks
SECTION B – continued
15 2016VETLABSKILLSEXAM
Question 12 (4marks)a. Whatismeantbytheterm‘samplingplan’inasepticanalysis? 1mark
b. Whyisitimportanttofollowasamplingplan? 1mark
c. Withregardtocollectingsamples,suggesttwoplaceswheredetailedsamplinginformationcanberecorded. 2marks
1.
2.
Question 13 (4marks)Anewlogbookneedstobesetupforalaboratory’sworkingsolutions.Informationaboutallthesolutionsmustbeincludedinthelaboratorylogbook.
a. Listtwocomponentsthatmustbeincludedinthelaboratorylogbookforaworking solution. 2marks
1.
2.
b. Astocksolutionof10Mhydrochloricacidwaspurchasedfromthelocalhardwarestore.
i. Whereshouldthecontainerbestored? 1mark
ii. Whatotherpieceofinformationfromthelabelmustberecordedinthelaboratorylogbook?(Youmaynotuseanswersfrompart a.) 1mark
SECTION B – continuedTURN OVER
2016VETLABSKILLSEXAM 16
Question 14 (7marks)a. Whatisa‘controlblank’inmicrobiologicalanalysisandwhyisitrequired? 2marks
b. Wherecouldthecriteriaforacceptanceoftheresultsfromacontrolblankbefound? 1mark
c. Someresultsobtainedfromacontrolblankwereattheouterlimitoftheacceptableguidelines.
Suggesttwofactorsthatmayhaveresultedinthishappening.Explainhoweachfactorwouldcausetheresultsthatwereobserved. 4marks
Factor1
Explanation
Factor2
Explanation
SECTION B – continued
17 2016VETLABSKILLSEXAM
Question 15 (4marks)Suggestwhatiscommonlytreatedbyeachofthefollowingdisposalmethods.
• Add0.5%hypochloritesolution.
• Soakin80%ethanolforfiveminutes.
• Exposetoultravioletlightinabiosafetycabinet.
• Autoclaveatthecorrecttemperatureandpressurefortherecommendedtime.
Question 16 (3marks)Maxisalaboratoryassistantwhohasjustreturnedtoworkafterbeingonsickleaveforacold.Heassumedhewouldbewellenoughtoreturntowork,despitehisoccasionalboutsofsneezing.Maxhasbeenaskedtotransferaclient’sthroatswabsamplesontomicroscopeslides.Maxhastheworkingareasetupcorrectlywiththeappropriateequipmentfortheprocedure.Heisalsowearingsafetyclothing,buthasforgottentowearafacemask.
a. WhatcouldhappentothesamplesontheslideswhenMaxpreparesthem? 1mark
b. GivenMax’sproceduraltechnique,wouldtheslidesstillbeviablesamples?Why? 1mark
c. Ifthesampleswerenotviable,whatshouldbedone? 1mark
SECTION B – continuedTURN OVER
2016VETLABSKILLSEXAM 18
Question 17 (9marks)Duringascheduledcheckofthelaboratorysolutions,itisfoundthatthestocksolutionof1.00Msodiumhydroxide(NaOH)usedforpreparingthesecondarystandardsolutionsisabouttoexpire.
a. Whatismeantbytheterm‘stocksolution’? 1mark
A1.00MNaOHsolutionhasbeenpreparedandatechnicianstandardisesthesolutionbytitrating 4×20mLaliquotsof1.00Mpotassiumhydrogenpthalate(KC8H5O4),aprimarystandard,withtheNaOH.NaOHreactswithKC8H5O4ina1:1ratio.Thetablebelowshowstheresultsobtainedfromthetitrationofthe1.00MKC8H5O4withthe1.00MNaOHsolution.
Titre number Titre volume (mL)
1 19.45
2 19.35
3 19.60
4 19.40
b. Calculatetheaveragetitrevalueusingconcordanttitrevalues.Showyourworking. 2marks
SECTION B – Question 17–continued
19 2016VETLABSKILLSEXAM
c. Usingthevaluecalculatedinpart b.,determinetheconcentration,inmol/L,oftheNaOHstocksolutiontothreesignificantfigures.Showyourworking. 3marks
d. Thelaboratoryspecificationsstatethatthestandardstocksolutionshouldbewithinarangeof5%oftheexpectedvalueof1.00MNaOH.
WouldthestandardstocksolutionofNaOHmadeupbythetechnicianbeaccepted?Justifyyouranswerusingyourresponsetopart c. 3marks
END OF QUESTION AND ANSWER BOOK