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VERIFICATION OF VERIFICATION OF BLOOD BLOOD
COLLECTION COLLECTION SYSTEMSYSTEM
GIAN LUCA SALVAGNO , MD, PhDGIAN LUCA SALVAGNO , MD, PhD
University of Verona,University of Verona,
ItalyItaly
HUBHUB
Spoke
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STARTSTART
LaboratoLaboratory’s ry’s errorserrors
Lundberg’s loop.Lundberg’s loop.
Laboratory Laboratory testingtesting
Laboratory Laboratory testingtesting
Errors and patient’s Errors and patient’s outcomeoutcomeErrors and patient’s Errors and patient’s outcomeoutcome
Pre-Pre-analytianalyti
cal cal errorserrors
Pre-Pre-analytianalyti
cal cal errorserrors
Preanalytical:Preanalytical: 56%56%Analytical:Analytical: 21%21%Postanalytical:Postanalytical: 18%18%Multiple:Multiple: 5%5%
Preanalytical:Preanalytical: 56%56%Analytical:Analytical: 21%21%Postanalytical:Postanalytical: 18%18%Multiple:Multiple: 5%5%
……………….and in .and in laboratory?laboratory?
Pre-analytical phase: Where?Pre-analytical phase: Where?Patient identificationPatient identification
CriticalCriticals points pointCriticalCriticals points point
Blood specimen collectionBlood specimen collection
Specimen identificationSpecimen identification
Specimen transportSpecimen transport
Time of specimen arrivalTime of specimen arrival
Check-inCheck-in
Centrifugation decapingCentrifugation decaping
Aliquoting and labeling of the Aliquoting and labeling of the secondary specimensecondary specimen
OutsideOutside
LLaabboorraattoorryy
WithinWithin
Phlebotomist?Phlebotomist?
Blood collection deviceBlood collection device
Blood Blood drawing drawing
techniquetechnique
VENOUS STASISVENOUS STASIS
Venous Venous Stasis:Tourniquet Stasis:Tourniquet timetime
1)Tourniquet time: Clinical 1)Tourniquet time: Clinical ChemistryChemistry
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2) Tourniquet time: 2) Tourniquet time: Coagulation testingCoagulation testing
3) Tourniquet time: 3) Tourniquet time: Hematological testingHematological testing
BLOOD CONTAINERBLOOD CONTAINER
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AnticoagulanAnticoagulants ts
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VENOUS ACCESSVENOUS ACCESS
Hemolysis:Hemolysis:
Clinical Clinical Chemistry Chemistry
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Hemolysis:Hemolysis:
Coaguation Coaguation testingtesting
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VisuallyVisuallyundetectableundetectablehemolysishemolysis
VisuallyVisuallyundetectableundetectablehemolysishemolysis
Percentage RBC lysate on the total RBC mass.Percentage RBC lysate on the total RBC mass.0 0.1 0.2 0.4 0.8 1.6 3.3 6.8 14.70 0.1 0.2 0.4 0.8 1.6 3.3 6.8 14.7
HemolyHemolysis:clinisis:clini
cal cal chemistchemist
ry ry testingtesting
NEEDLE SIZENEEDLE SIZE
Recommendations for Collection of Recommendations for Collection of a Quality Specimen for Coagulation a Quality Specimen for Coagulation
TestingTesting
Recommendations for Collection of Recommendations for Collection of a Quality Specimen for Coagulation a Quality Specimen for Coagulation
TestingTesting
Influence of under-filling of 3.2% buffered sodium citrate blood Influence of under-filling of 3.2% buffered sodium citrate blood tubes on results of activated partial thromboplastin time tubes on results of activated partial thromboplastin time (APTT),(APTT),prothrombin time (PT), fibrinogen, activated protein C resistance prothrombin time (PT), fibrinogen, activated protein C resistance
(APCR), and coagulation factor VIII. The dotted lines designate (APCR), and coagulation factor VIII. The dotted lines designate the desirable biasthe desirable bias
for each parameter.for each parameter.
Influence of under-filling of 3.2% buffered sodium citrate blood Influence of under-filling of 3.2% buffered sodium citrate blood tubes on tubes on results of activated partial thromboplastin time (APTT),results of activated partial thromboplastin time (APTT),prothrombin time (PT), fibrinogen, activated protein C resistance prothrombin time (PT), fibrinogen, activated protein C resistance
(APCR), and coagulation factor VIII. The dotted lines designate (APCR), and coagulation factor VIII. The dotted lines designate the desirable biasthe desirable bias
for each parameter.for each parameter.
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The The JCAHOJCAHO has recently given priority within the has recently given priority within the 2007 2007 National Patient Safety GoalsNational Patient Safety Goals to the to the
improvement of the accuracy of patient improvement of the accuracy of patient identification and it applies to all JCAHO-accredited identification and it applies to all JCAHO-accredited healthcare organizations and those seeking JCAHO healthcare organizations and those seeking JCAHO
accreditation. Failure to comply can result in a accreditation. Failure to comply can result in a special Type I recommendation and jeopardize a special Type I recommendation and jeopardize a
facility’s accreditation status.facility’s accreditation status.
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The The International Organization International Organization for Standardizationfor Standardization 15189:2003 15189:2003
clause 5.4 “clause 5.4 “Pre-examination Pre-examination procedures'procedures'” includes ” includes
requirements for traceability of requirements for traceability of primary samples to an identified primary samples to an identified
individual.individual.
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What’s next?What’s next?
““Which is the best choice?”Which is the best choice?”
Or……Or……
……and I am ready to start!and I am ready to start!
The EndThe End
Take a home message