VLA Weybridge, New Haw, Addlestone, Surrey KT15 … · Catalogue 2010 11 e x p e r t s c i e n c e...

VLA is an Executive Agency of the Department forEnvironment, Food and Rural Affairs (Defra) VLAS5/04.2010/1

Safeguarding public and animal health

To contact VLA Scientific:Telephone: +44 (0)1932 357641 Facsimile: +44 (0)1932 357701Email: [email protected]

VLA Weybridge, New Haw, Addlestone, Surrey KT15 3NB United Kingdom

www.vlascientific.com Biological Reagents &Proficiency Testing

Catalogue 2010 11

e x p e r t s c i e n c e l e x c e l l e n t s e r v i c e

The Veterinary Laboratories Agency is a UKnetwork of veterinary laboratories and isinternationally recognised as a centre of scientificexcellence.VLA safeguards public and animal health byproviding all sectors of the animal health industrywith veterinary research, diagnostic services andanimal disease surveillance of farmed livestock andwildlife.

ReagCat1011_CovAW:Layout 1 13/04/2010 10:01 Page 1

The Veterinary Laboratories Agency is internationallyrecognised as a centre of scientific excellence and acts as anInternational Reference Laboratory for a wide range ofinfectious and non-infectious diseases in farm animals.

UK National Reference Laboratory for:

n Anthraxn Antimicrobial Resistance (animal samples)n Aujeszky's Diseasen Avian Influenzan Brucellosisn Campylobactern Classical Swine Fevern Enzootic Bovine Leukosisn Equine Encephalomyelitisn Equine Infectious Anaemian Newcastle Diseasen Salmonellan Teschen Disease (Porcine Viral

Encephalomyelitis)n Transmissible Spongiform

Encephalopathiesn Trichinella

UN Food and Agriculture Organisation(FAO) Reference Centre for:

n Avian Influenzan Brucellosisn Newcastle Diseasen Poultry Diseases

World Health Organisation (WHO)Collaborating Centre for:

n Characterisation of Rabies andRabies-related Viruses

n Reference and Research onBrucellosis

European Union (EU) CommunityReference Laboratory for:

n Avian Influenzan Newcastle Diseasen Transmissible Spongiform

Encephalopathies

World Organisation for Animal Health(OIE) Reference Laboratory for:

n Antimicrobial Resistance (animal samples)n Avian Influenzan Bovine Tuberculosisn Bovine Viral Diarrhoean Brucellosis n Classical Swine Fevern Contagious Agalactian Contagious Equine Metritisn Enzootic Bovine Leukosisn Equine Viral Arteritisn Infectious Bovine Rhinotracheitisn Newcastle Diseasen Rabiesn Salmonellosisn Scrapie and Bovine Spongiform

Encephalopathy


Contents

How to use this catalogue

1

VLA Scientific 2-3

VLA Scientific Biological Reagents 4

VLA Scientific Proficiency Testing (Vetqas) 5

General Information 6-7

Reagents - Bacterial Diseases 8-14

VLA and Identibac Bacterial Genotyping 12

VLA and Pro-Lab Diagnostics 13

VLA Scientific Antimicrobial Testing 15

Reagents - Viral Diseases - Avian 16-21

VLA and Applied Biosystems 20

Reagents - Viral Diseases - Mammalian 22-26

VLA and Qiagen 24

TuffTM RNA - RT PCR Controls 25

Reagents - Miscellaneous 27

VLA Scientific and Phengenix Cell Characterisation 28

VLA Scientific Laboratory Testing Service 29

Proficiency Testing Schemes (Vetqas) 30-32

VLA Worldwide Distributors 33

Index 34-36

The reagents in this catalogue are divided into four maincategories:

Bacterial DiseasesViral Diseases - AvianViral Diseases - MammalianMiscellaneous

Each of the four categories is sub-divided into four groups:

AntibodiesAntigensAntiseraDiagnostic Kits

32

The Veterinary Laboratories Agency (VLA) has a mission to safeguardpublic and animal health, protect the economy and enhance food securitythrough world class veterinary research and surveillance.

The Agency has a rich scientific history dating back over 100 years and hascontributed to many of the milestones in the veterinary landscape over the lastcentury. It is an International Reference Laboratory for many important diseasesand VLA scientists have had a significant influence on the development of animalhealth and food safety strategies in the UK and the rest of the world.

These achievements have earned the VLA an international reputation for scientificexcellence as it has led the world in developing new techniques for the diagnosisand management of animal disease.

To enhance its scientific activity in recent years, VLA has developedcommercial products and services which have now been brought togetherunder VLA Scientific.

VLA Scientific includes business development managers, a customer serviceteam, scientific research and reagent production groups to ensure the productsand services offered are delivered effectively to our customers. They are also ableto bring scientific expertise, high quality service, innovative methods and, in manycases unique products and services to the market.

The products and services available include:

n Specialist laboratory reagents & diagnostic kits

n A world leading range of proficiency testing schemes

n Testing services for veterinary practitioners and the pharmaceutical industry

n Disinfectant testing

n Veterinary research and development projects

n Risk analysis and data science services

n A unique cell characterisation serviceTo contact VLA Scientific: Telephone: +44 (0)1932 357641

Facsimile: +44 (0)1932 357701 Email: [email protected] visit www.vlascientific.com

Products and servicesfor scientists

around the world


VLA Scientific

VLA has developedcommercial products andservices which have nowbeen brought together underVLA Scientific.

VLA Scientific Biological Reagents

VLA has over 100 years experience in the diagnosisand control of important diseases of farm livestockand has developed many diagnostic tests. We offer arange of specialist laboratory reagents anddiagnostic kits for the testing of bacterial, viral andTSE infections. These reagents are available for saleto the animal health industry and veterinarydiagnostic laboratories worldwide.

VLA Scientific ProficiencyTesting Services

The Quality Assurance Unit - Vetqas is based at SuttonBonington, near Loughborough, Leicestershire (on the same siteas the Regional Laboratory). It is the market leader in proficiencytesting for veterinary laboratories, with over 30 years experience.

PT demonstrates that laboratory testing is beingperformed to a high standard and when doneregularly provides a tool to:

n demonstrate the accuracy of results

n check the efficiency of quality control measures

n select optimal methodology

n maintain the quality of tests

n compare results with other organisations

The schemes we offer are internationally recognisedand accepted by accreditation bodies for laboratoryaccreditation purposes, e.g. for ISO17025.

Schemes are available in the following areas:

Antibody Detection TSE

Microbiology Virology

Haematology Parasitology

Clinical Chemistry Feed Tests

For further information on Proficiency Testing Schemes see pages 30/32or contact Vetqas at:

Veterinary Laboratories Agency Quality Assurance UnitCollege Road, Sutton BoningtonLoughborough, Leicestershire LE12 5RB United Kingdom

Telephone: +44 (0)1509 670607 Facsimile +44 (0)1509 670206Email: [email protected]

or visit www.vla.gov.uk/eqa/eqa-home.htm

reagents PT gives you and yourcustomers the confidence thatyour testing is accurate

54

for quality testing

The management systems in our manufacturingfacility are certified to ISO9001: 2008.

Our specialist reagents enable customers toperform their own tests. However, VLA also hasa Veterinary Diagnostic Testing Service ifrequired (see page 29).

Quality

For further information please contact:

VLA Scientific Product SalesVeterinary Laboratories AgencyNew Haw, Addlestone, Surrey, KT15 3NB United Kingdom

Telephone: +44 (0) 1932 357641 Facsimile +44 (0) 1932 357701Email: [email protected]

A product catalogue is available on-line atwww.vlascientific.com

NEWSCHEMESfor 10/11

Orders

When ordering please identify all items using the catalogue numbere.g. RAA0066 and a description of the items.

Telephone orders will be accepted but must be confirmed by fax or e-mail within 48hours, and be marked 'confirmation of telephone order'. Your order numbershould be quoted on all correspondence following confirmation of order.

Please give the full address for delivery (and for the invoice where this is different)including telephone, fax number and email address.

Reagent orders are processed either by Product Sales or by a Distributorappointed by VLA.

If you reside in a country without an appointed Distributor please contact ProductSales (details below).

If you reside in a country with an appointed Distributor please contact the distributor.A list of appointed VLA Worldwide Distributors and the regions they covercan be found on page 33.

OVERSEAS CUSTOMERS (from countries without Distributors) MUST ENSURETHAT ALL NECESSARY IMPORT PERMITS ACCOMPANY THE ORDER.

THE CONDITIONS OF SALE APPLY TO ALL ORDERS RELATING TO PRODUCTSAND PROFICIENCY TESTING. PLEASE CONTACT PRODUCT SALES FOR DETAILSOF VLA’S STANDARD TERMS AND CONDITIONS OR SEE OUR WEBSITE.

Orders should be sent to:

VLA Scientific Product Sales, Veterinary Laboratories AgencyNew Haw, Addlestone, Surrey KT15 3NB United KingdomTelephone +44 (0)1932 357641Facsimile +44 (0)1932 357701Email: [email protected]

Technical Services and Advice

Documentation is supplied with all catalogue products, and technical services andadvice are also available. In addition to the products listed in this catalogue VLA has awide range of research grade biological products for laboratory use. If you have anyspecial requirements or require further advice, please contact Product Sales at theabove address.

Prices

Prices quoted in the Price List are for the UK only and are valid from 1 April2010 to 31 March 2011.

The price charged will be that prevailing at the time of despatch.

All prices are exclusive of VAT, which will be added to UK orders at the rateprevailing at the time of the invoice. For EC orders please provide VAT registrationnumber otherwise VAT will be charged at the UK prevailing rate.

Delivery charges are not included in the Price List and will be added at cost.

All and any import duties, value added taxes or other charges of whatever natureleviable or chargeable on the goods on importation into the country of destinationshall be paid by the Overseas Buyer.

We reserve the right to amend prices without notification.

General Information

Payment

Payment is due within 30 days on receipt of the invoice, in pounds sterling, bybankers order or cheque drawn on a UK clearing bank made payable to 'VeterinaryLaboratories Agency'. Cheques must show the UK Bank Sort Code. Payment is alsoavailable through BACS. VLA's bank details for payment by this method are availableon request from Product Sales at VLA. Payment can also be made by Credit/DebitCard. All payment details are shown on page 2 in the Biological Reagents andProficiency Testing - Terms and Conditions of Sale. See our Website.

In some cases it will be necessary to clear payment before despatch.

UNLESS OTHERWISE STATED, AND AGREED IN WRITING, PROPERTY AND TITLE OF THE GOODS WILL NOT PASS TO THE CUSTOMER UNTIL THE WHOLE PRICE HAS BEEN RECEIVED BY US.

Returns

Biological reagents are not generally accepted for return. Should the need arise,please contact Product Sales (at the address on page 6) before sending any goods forreturn.

Product Liability

Products listed are intended for use as in-vitro animal diagnostics. Those products foruse in veterinary in-vivo diagnostic procedures or disease prophylaxis are clearlyspecified. It is the responsibility of the user to ensure that they possess the necessarytechnical skills to determine the appropriateness of these products for the proposedapplication. VLA accepts no responsibility for results obtained from these products, asthey are also dependent on conditions of use and the variability of materials beyondour control.

In no event shall VLA be held responsible for the loss of profits, or for indirect orconsequential loss including, but not limited to, personal injury arising from the use ofthese products.

Customers are advised that VLA accepts no responsibility for the non-deliveryof goods, or for damages in transit unless it can be proved to have resultedfrom our negligence. Claims for goods damaged in transit should be madewithin three working days of receipt. Any replacements are made strictly asan act of goodwill, and should in no way be construed as an acceptance ofliability on our part.

Live and Infectious Materials

Many of the products are infectious. Please ensure that:

n all necessary precautions are taken when handling live infectious material

n full operator safety precautions are observed when handling material which may beinfectious to humans

n operators are fully informed as to the nature of the material they are handling

n instructions for use and safe disposal are carried out

n overseas orders are accompanied by all the import documents necessary for this class of materials

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Proficiency Testing Services are available for reagents marked PT.See page 5 for details or pages 30/32 for the PT Schemes.PT

Diagnostic Kits

RAI0990 BRUCELISA-160 PT

RAI0705 BRUCELISA-400 PT

An indirect ELISA kit for the detection of circulating antibodies to Brucella abortus inthe serum of bovine blood samples. BRUCELISA-160 contains sufficient reagents,including antigen coated plates, to test up to 160 samples and BRUCELISA-400 up to400 samples.

RAI2017 BRUCELISA-160SG

RAI2018 BRUCELISA-400SG

An indirect ELISA kit for the detection of circulating antibodies to Brucella melitensis inthe serum of sheep and goat blood samples. BRUCELISA-160SG contains sufficientreagents, including antigen coated plates, to test up to 160 samples and BRUCELISA-400SG up to 400 samples.

RAI0991 BRUCELISA-160M PT

An indirect ELISA kit for the detection of antibodies to Brucella abortus in bovine milksamples. Each kit contains sufficient reagents, including antigen coated plates, to testup to 160 samples.

RAI2005 COMPELISA-160 PT

RAI2006 COMPELISA-400 PT

A competition ELISA kit for the detection of circulating antibodies to Brucella infectionin the serum of all animal species. COMPELISA-160 contains sufficient reagents andstandard sera to test 160 samples and COMPELISA-400 contains sufficient reagentsand standard sera to test 400 samples.

Antigens

RAA0066 Brucella abortus/melitensis/suis Complement Fixation Test 100ml PT

Inactivated antigen for use in the Complement Fixation Test for the detection ofantibodies to B.abortus/melitensis/suis. Working dilution x10 concentrate. Volume permicrotitre well 25�l. Standardised to give 50% fixation with a 1/200 dilution of theInternational Standard Serum to B.abortus. Liquid presentation; store at 4°C in thedark.

RAA0048 Brucella abortus Milk Ring Test 100ml PT

Inactivated antigen for use in the Milk Ring Test for the detection of antibodies toB.abortus. 30�l required per test. All batches are adjusted to have a comparable levelof sensitivity when tested against dilutions made in milk of the International StandardSerum to B.abortus. Liquid presentation; store at 4°C in the dark.

RAA0060 Brucella Rose Bengal Test (Standard) 100ml PT

Inactivated antigen for use in the Rose Bengal Test for the detection of antibodies toall smooth strains of Brucella. The antigen is standardised to give a positive reactionwith a 1/45 dilution of the International Standard Serum (OIEISS) (30�l per test).Liquid presentation; store at 4°C in the dark.

RAA2016 Brucella Rose Bengal Test (Sensitive) 100ml PT

Inactivated antigen for use in the Rose Bengal Test for the detection of antibodies toall smooth Brucella strains and recommended for the detection of Brucella antibodiesin sheep and goats. Distribution in the UK is restricted to authorised laboratories. Theantigen is standardised to give positive reaction with a 1/47.5 dilution of theInternational Standard Serum (OIEISS) (30�l per test).Liquid presentation; store at 4°C in the dark.

RAA0054 Brucella abortus/melitensis/suis Serum Agglutination Test 100ml PT

Inactivated antigen for use in the Serum Agglutination Test for the detection ofantibodies to B.abortus/melitensis/suis. Working dilution x10 concentrate. At workingstrength the antigen is standardised to give 50% agglutination with a 1/650 dilutionof the International Standard Serum to B.abortus. Volume per tube, 500�l, volumeper microtitre well, 50�l. Liquid presentation; store at 4°C in the dark.

Bacterial Diseases Bacterial Diseases

Antisera

RAB0701 Brucella abortus negative control serum 1ml PT

Freeze-dried presentation; store at 4°C. Bovine origin.

RAB1003 Brucella abortus positive control serum 1ml PT

Compared against the International Standard Serum to B.abortus.B.abortus positive control serum for monitoring diagnostic tests. Bovine origin.Freeze-dried presentation; store at 4°C.

RAB0974 Brucella melitensis positive control serum 1ml PT

Compared against the International Standard Serum to B.abortus.Used for monitoring diagnostic tests. Caprine origin. Freeze-dried presentation; storeat 4°C.

RAB0075 Brucella ovis negative control serum 1ml PT

Freeze-dried presentation; store at less than 10°C. Ovine origin.

Brucella

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To order: Contact: VLA Scientific Product Sales Tel +44 (0) 1932 357641 Fax +44 (0)1932 357701 or Email: [email protected] your Distributor (see page 33)

Antibodies - Monoclonal mAb immunoglobulin

class

RAE0234 Clostridium perfringens � toxin IgG1 1mg

Specific for the epsilon toxin of C.perfringens and intended for use in assays tomeasure neutralising antibody responses following vaccination. Murine origin; affinitypurified hybridoma culture supernatant concentrated to 1mg/ml. Freeze-driedpresentation; store at 4°C.

Campylobacter

Escherichia coli

Clostridium

Antisera - PolyclonalA comprehensive range of antisera for use in Slide Agglutination Tests for thepresumptive identification of pathogenic E.coli from birds, cows or pigs. All E.coliantisera contain agglutinins to both ‘O’ and ‘K’ antigens. Sera are of rabbit origin andare presented in liquid form ready to use. Store at 4°C.

Avian Antisera

RAB0976 E.coli O1:K1 typing serum OK ‘F1’ (avian strain) 1ml

RAB1002 E.coli O2:K1 typing serum OK ‘F21’ (avian strain) 1ml

RAB0977 E.coli O78:K80 typing serum OK ’F103’ (avian strain) 1ml

Bovine Antisera

RAB0978 E.coli O78:K80 typing serum OK ‘RVC330’ 1ml

Antibodies - MonoclonalmAb immunoglobulin

class

RAE0235 E.coli K88a fimbrial adhesin IgG1 1mg

RAE0236 E.coli K88b fimbrial adhesin IgG1 1mg

RAE0237 E.coli 987P fimbrial adhesin IgG3 1mg

RAE0238 E.coli F41 fimbrial adhesin IgG2b 1mg

RAE0239 E.coli K99 fimbrial adhesin IgG2a 1mg

For specific detection and identification of the fimbrial adhesins in animal isolates ofE.coli.

Porcine Antisera

Polyvalent A and constituents: All 1ml

RAB0086 E.coli Polyvalent A typing serum

RAB0089 E.coli G205 typing serum O8:K87,K88ac

RAB0090 E.coli E65 typing serum O45:K ‘E65’

RAB0091 E.coli E57 typing serum O138:K81

RAB0092 E.coli E6811 typing serum O141:K85ab

RAB0093 E.coli E145 typing serum O141:K85ac

RAB0094 E.coli G1253 typing serum O147:K89,K88ac

RAB0095 E.coli Abbotstown typing serum O149:K91,K88ac

Polyvalent B and constituents: All 1ml

RAB0087 E.coli Polyvalent B typing serum

RAB0096 E.coli P16 typing serum O9:K103,987P

RAB0097 E.coli V142 typing serum O64:K ‘V142’


RAB0099 E.coli E4 typing serum O139:K82


Polyvalent C and constituents: All 1ml

RAB0088 E.coli Polyvalent C typing serum




RAB0104 E.coli V113 typing serum O119:K’V113’

RAB0975 E.coli K87 typing serum containing antibodies to E.coli antigen D271(O2:K87)

RAB0108 E.coli K91 typing serum containing antibodies to E.coli antigen K91



Diagnostic KitsFIMBREX kits combine latex agglutination technology with monoclonal antibodyspecificity to provide rapid, easy-to-perform tests for the presence of fimbrial adhesinson cultures of enterotoxigenic E.coli (ETEC). Coloured latex makes test results easy toread and end-points are reached in a few minutes. Each kit is specific for one of thefimbrial adhesins and contains disposable agglutination test cards and reagents for 60tests performed individually, or 90 tests when performed in multiples of three. Inaddition to the monoclonal antibody-coated latex, the kit also contains a controlreagent to detect non-specific agglutination, an inactivated positive control antigen,and suspension buffer, all supplied in convenient dropper bottles; only mixing sticksare required in order to carry out the test. Reagents are presented in liquid form andare ready for use. Store at 4°C.

RAI0261 FIMBREX K99

RAI0262 FIMBREX F41

RAI0263 FIMBREX K88

RAI0264 FIMBREX 987P


Kit for Transportation of Samples

RAI0800 Campyculture Kit

The methodology and transport enrichment media were developed at VLA for thesuccessful sampling and culturing of Campylobacter fetus spp. from bulls and cows.The kit contains a filter and sufficient reagents for sampling one bull or two cows.Each kit is supplied with a set of user instructions, a leaflet providing details ofcontents together with two laboratory sample submission forms. It should be notedthat insertion tubes and syringes are not supplied. This kit can also be used for thetransport of Trichomoniasis samples.

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1312

If you require further information or wish to place an order,please contact:

VLA Scientific produces an extensive rangeof CE marked

SalmonellaAgglutination TypingAntisera

Pro-Lab DiagnosticsUnit 7, Westwood Court, Clayhill, Neston, South Wirral, Cheshire CH64 3UJ, United Kingdom

Telephone +44 (0)151 353 1613Fax +44 (0)151 353 1614Email: [email protected]

www.pro-lab.com

The antisera, for in-vitro diagnostic use, are availablefrom Pro-Lab Diagnostics and are presented in 3mlcolour-coded dropper bottles and have shelf lives of upto 5 years from date of manufacture.

The range of antisera includes a full panel of:

l polyvalent somatic l monovalent somaticl polyvalent flagellal monovalent flagellafor traditional slide agglutination testing

Mycobacteria

Salmonella

Diagnostic Kit

RAI5047 Mycobacterium bovis - Brock TB Stat-PakTM

A rapid serological test kit (manufactured by Chembio Diagnostics Inc) to detectantibodies to Mycobacterium bovis (TB) in badgers. The kit contains 10 lateral flowdevices, which can be used in the field or the laboratory. Serum from the badger ismixed with the diluent supplied and placed on the sample pad of the device.A positive result shows in the result window as a blue line within 5 minutes.

Mycoplasma

Diagnostic Kits

RAI6223 BoviLAT

A latex agglutination test for the serological detection of Contagious BovinePleuropneumonia (CBPP). Can be used on the farm or in the laboratory. Store at 4°C.There is sufficient reagent for 100 tests.

RAI6224 CapriLAT

A latex agglutination test for the serological detection of Contagious CaprinePleuropneumonia (CCPP). Can be used on the farm or in the laboratory. Store at 4°C.There is sufficient reagent for 100 tests.

Antigens

RAA0191 Salmonella Pullorum stained antigen 25ml

Inactivated antigen for use in the Rapid Slide Agglutination Test. Contains bothstandard and variant strains of S.Pullorum stained with crystal violet and suspended inbuffered formol saline. There is sufficient reagent for 500 tests. Store at 4°C - do notfreeze.

Antisera PT

An extensive range of CE marked Salmonella agglutination typing antisera, for in-vitrodiagnostic use, can be obtained from PRO-LAB DIAGNOSTICS (see page 13).The range includes a full panel of polyvalent somatic, monovalent somatic, polyvalentflagella and monovalent flagella antisera for traditional slide agglutination testing.



VLA Scientific Antimicrobial Testing

over30,000

bacterial isolates

VLA Scientific is able to provide up-to-date susceptibilityprofiles for a wide range of currently marketed or researchantimicrobials. We can carry out comparative studies betweendifferent antimicrobials and changes in resistanceprofile over time, which may be suitable for productregistrations, claim extensions or marketingpurposes.

To support these services we maintain an extensivecollection of over 30,000 recent and historicbacterial isolates of large animal pathogens, which includesboth common and less common aerobes and anaerobes fromall livestock species.

Some of these isolates already have establishedsensitivity profiles, so we can selectively testorganisms of known provenance. We can alsoassemble collections of recent isolates for testing ifthe above do not fulfil your requirements.

We can also collect isolates to your requirementsif you have a specific need not covered by thecurrent collection.

For further information please contact:VLA Bury St EdmundsRougham Hill, Bury St Edmunds, Suffolk IP33 2RXTelephone +44 (0)1284 724499 Facsimile +44 (0)1284 724500Email: [email protected] visit the VLA Scientific website www.vlascientific.com

Storage facility for bacterial isolates

1514

Viral Diseases - Avian

Avian Adenovirus (CELO)

Antigen

RAA0026 Avian Adenovirus (CELO) 0.5ml

Live antigen prepared for use in the Gel Immunodiffusion Test. Grown in thechorio-allantoic membrane of Specific Pathogen Free (SPF) hen eggs. Freeze-driedpresentation; store at 4°C.

Antisera

RAB0027 Avian Adenovirus (CELO) positive serum 0.5ml

Chicken antiserum to avian adenovirus (CELO) for use as a positive reference serum inthe Gel Immunodiffusion Test. Freeze-dried presentation; store at 4°C.

Avian Paramyxovirus (PMV 3)

Antisera

RAB0033 PMV 3 positive serum 0.5ml

Chicken antiserum to avian paramyxoviruses sub-type 3. Prepared as a positive controlserum to be used in the Haemagglutination Inhibition Test. Freeze-dried presentation;store at 4°C.Other subtypes are available for commercial supply but they are strictlylimited. Please contact Product Sales for details.

Avian Reovirus

Antigen

RAA0028 Avian Reovirus 0.5ml

Live antigen prepared for use in the Gel Immunodiffusion Test. Grown in the chorio-allantoic membrane of SPF hen eggs. Freeze-dried presentation; store at 4°C.

Antisera

RAB0029 Avian Reovirus positive serum 0.5ml

Chicken antiserum to avian reovirus for use as a positive reference serum in the GelImmunodiffusion Test. Freeze-dried presentation; store at 4°C.

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Viral Diseases - Avian

Egg Drop Syndrome ’76

Antigen

RAA0081 Egg drop syndrome ‘76 0.5ml

Live antigen produced by growth of the EDS ‘76 virus in the allantoic sac of duck eggsand harvested as allantoic fluid. Intended for use as an antigen in theHaemagglutination Inhibition Test. Freeze-dried presentation; store at 4°C.

Antisera

RAB0082 Egg drop syndrome ‘76 positive serum 0.5ml

Chicken antiserum to EDS ‘76 virus for use as a positive reference serum in theHaemagglutination Inhibition Test. Freeze-dried presentation; store at 4°C.

Infectious Bronchitis Virus PT

Antigens

RAA0121 Infectious bronchitis virus M41 0.25ml

RAA2003 Infectious bronchitis virus CVL793/B 0.25ml

RAA0715 Infectious bronchitis virus D274 0.25ml

RAA0716 Infectious bronchitis virus D1466 0.25ml

RAA5041 Infectious bronchitis virus 755/03 0.25ml

Live antigen produced by growth of the specified strain of infectious bronchitis virus inthe SPF hen eggs and harvested as allantoic fluid. Enzyme treated; intended for use asantigen in the Haemagglutination Inhibition Test. Freeze-dried presentation; store at4°C.

Antisera

RAB0122 Infectious bronchitis virus positive serum M41 0.5ml

RAB2004 Infectious bronchitis virus positive serum CVL793/B 0.5ml

RAB5040 Infectious bronchitis virus positive serum 755/03 0.5ml

Chicken antiserum to infectious bronchitis virus for use as a positive reference serumin the Haemagglutination Inhibition Test. Freeze-dried presentation; store at 4°C.


Viral Diseases - Avian Viral Diseases - Avian

1918

If you would like further details regarding product availability or to discussyour particular requirements or to place an order please contact:

VLA Scientific Product Sales, Veterinary Laboratories AgencyNew Haw, Addlestone, Surrey KT15 3NB United KingdomTelephone +44 (0)1932 357641 Facsimile +44 (0)1932 357701Email: [email protected]: www.vlascientific.com

VLA is an InternationalReference Laboratory (IRL)for Avian Influenza andNewcastle Disease.

Avian Influenzahelping in the worldwidefight against

It may be possible to initially screen all haemagglutinatingagents with the primary serum. However, to achieve a definitiveidentification of the virus subtype, a second serum (produced toa virus with a heterologous neuraminidase subtype) should beused to ensure accurate identification and eliminate any crossreactivity due to a common neuraminidase subtype betweenstrains.For serology, consistent with the principles for virusidentification, one antigen should be used for screening and allpositive reactors confirmed using a second antigen in order toeliminate interference from cross neuraminidase antibodies.

Virus ID Virus ID Serology Serology primary secondary primary secondaryserum serum antigen antigen

H5 H5N1 H5N2 H5N1 H5N2RAB 7000 RAB 7001 RAA 7002 RAA 7003

H7 H7N7 H7N1 H7N7 H7N1RAB 7005 RAB 7004 RAA 7007 RAA 7006

It is important to ensure that reagents supplied are fitfor purpose and appropriate at a global level. For theidentification of Avian Influenza A virus (subtypes H5and H7) we recommend the reagents listed below.

A full VLA statement on a virus identification service,serology and VLA’s role as the WRL is available at:

www.vla.gov.uk(see Tests and Services)


Viral Diseases - AvianViral Diseases - Avian

20 21

Infectious Bursal Disease (Gumboro Disease) Virus PT

Antigen

RAA0123 Infectious bursal disease virus 1ml

Live antigen (Type 1) produced from the bursae of infected SPF chickens. Prepared foruse as antigen in the Gel Immunodiffusion Test. Freeze-dried presentation; store at4°C.

Antisera

RAB0124 Infectious bursal disease virus (type 1) positive serum 0.5ml

Chicken antiserum to infectious bursal disease virus (type 1) for use as a positivereference serum in the Gel Immunodiffusion Test. Freeze-dried presentation; store at4°C.

Marek’s Disease Virus

Antigen

RAA0156 Marek’s disease virus 1ml

Live antigen for Gel Immunodiffusion Test (HPRS-16). Freeze-dried presentation; storeat -20°C.

Antisera

RAB0157 Marek’s disease virus positive serum 0.5ml

For use as a positive control serum for the Gel Immunodiffusion Test. Freeze-driedpresentation; store at -20°C.

Newcastle Disease Virus

Antigen

RAA0158 Newcastle disease virus 1ml

Inactivated antigen for Haemagglutination Inhibition Test. Freeze-dried presentation;store at 4°C.

Antisera

RAB0155 Newcastle disease virus positive serum 0.5ml

Chicken antiserum to Newcastle disease virus for use as a positive reference serum inthe Haemagglutination Inhibition Test. Freeze-dried presentation; store at 4°C.


VLA andApplied Biosystems

VLA TaqMan®Influenza Detection Kits

When testing for Avian Influenza virus A (AIV), laboratoriesaround the world require rapid and reliable assays forinfluenza surveillance and to help enable immediate responsein the case of an outbreak.

In collaboration with the Veterinary Laboratories Agency, World ReferenceLaboratory for Avian Influenza, Applied Biosystems has developed a seriesof new and optimised VLA TaqMan® Influenza Detection Kits.

The kits provide superior detection accuracy, sensitivity and specificity foravian influenza A viruses and the H5 and H7 subtypes. Extensive validationhas been performed by the VLA with samples from multiple wild birdspecies and poultry samples. Furthermore, the assays are in an easy-to-uselyophilised bead format, which significantly simplifies the workflow,reduces samples-to-result time and improves the overall reproducibility ofavian influenza detection.

Detection Kit (DK) details:

n DK-Adetects all avian influenza A virus subtypes (H1 through to H16) from all geographic regions

n DK-H5detects Eurasian H5 avian influenza virus subtypes

n DK-H7detects Eurasian H7 avian influenza virus subtypes

Karl MeyerSenior Product Manager, Food & Environmental TestingApplied Biosystems Inc.850 Lincoln Centre Drive, Foster City, CA 94404, USATelephone +1 650 638 6329 Email: [email protected]

For further information please contact:

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. The kits are not approved for sale inthe United States.

© 2010, Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of LifeTechnologies Corporation or their respective owners. TaqMan® is a registered trademark of Roche Molecular Systems, Inc.

Bovine Viral Diarrhoea / Border Disease Viruses

Antibodies - Monoclonal (mAb) - Murine origin Pan-pestivirus reactive monoclonal antibodies PT

mAb antigen immunoglobulin detectsclass

RAE0801 WB103 NS2-3 IgG1 virtually all pestivirus 1mg

WB105 NS2-3 IgG2a virtually all pestivirus 1mg

A pool of two non-competing monoclonal antibodies, which have reacted with allpestiviruses tested at VLA to date, including BVD, CSF and BD viruses as well asuncharacterised viruses from other ruminants. Suitable for indirect labelling techniquesfor detection of pestivirus antigens in cell cultures and tissue sections. Store at 4°C.

Bovine viral diarrhoea virus (BVDV) monoclonal antibodies PT


RAE2020 WB103 NS2-3 IgG1 all pestivirus 1mg

WB112 NS2-3 IgG2a all pestivirus 1mg

WB166 E2 IgG1 BVDV type 1 & BVD 1mg

WB214 E2 IgG1 BVDV type 1 1mg

A pool of four monoclonal antibodies, which detects a wider range of BVDV antigensin tissue cultures. This pool is preferable to RAE0801 for detecting bovinepestiviruses. Store at 4°C.

Bovine viral diarrhoea (BVD) and Border disease (BD) virus monoclonal antibodies PT


RAE0822 WB162 E2 IgG1 BVDV-1 1mg

RAE0823 WB210 Erns IgG1 BVDV-1 1mg

RAE0824 WB215 E2 IgG2b BVDV-1 1mg

RAE4174 WB214 E2 IgG1 BVDV-1 1mg

RAE0241 WB160 NS2-3 IgG1 BVDV-1 & BDV 1mg

RAE4173 WB166 E2 IgG1 BVDV-1 & BDV 1mg

RAE0993 WS538 E2 IgG2a BVDV-2 1mg

RAE0992 WS433 Erns IgG2a BVDV-2 & BDV 1mg

RAE0825 WS363 Erns IgG1 BDV 1mg



Ruminant pestiviruses can be divided into BVDV1, BVDV2 and BDV. No singlemonoclonal antibody binds specifically to all three subtypes but the above panel takentogether will recognise most strains and indicate to which subtype they belong. Noneof these monoclonal antibodies bind to the CSF virus, so the panel may be used inconjunction with RAE0242 or RAE0826 (see page 23) for the differentiation ofBVD/BD and CSF isolates from pigs as recommended by the EC. Store at 4°C.Further discriminatory mAbs are available on request. Please contact Product Sales.

References:Edwards S, Sands J J, Paton D J (1991) Vet. Microbiol 29, 101-108.Paton D J, Sands J J, Edwards S (1994) Arch.Virol. 135, 241-252.Edwards S, Sands J J, Harkness J W (1988) Arch.Virol. 102,197-206.

Viral Diseases - Mammalian

2322


Please see page 23 for CSF mAbs


Antibodies -Fluorescein Isothiocyanate Conjugate (FITC)

RAC0205 FITC Ig to Bovine Viral Diarrhoea virus 2ml PT

This antibody is intended for the in-vitro detection of specific viral antigens in frozentissue sections, cytospin preparations and in cell cultures. The conjugates are preparedby joining the immunoglobulin fraction of hyperimmune antiserum to fluoresceinisothiocyanate. Non-specific fluorescence is removed by gel filtration and ionexchange chromatography. Freeze-dried presentation; store at 4°C.After reconstitution store at -20°C. Working dilution at least 1/10.

Antisera

RAB0042 Bovine Viral Diarrhoea virus positive serum 2ml PT

For use as a positive control for serum antibody assays, in antigen detectionprocedures and within research applications. Hyperimmune bovine serum derived fromnew born calves. Freeze-dried presentation; store at 4°C or at -20°C afterreconstitution.

Classical Swine Fever Virus PT

Antibodies - Monoclonal

Classical Swine Fever (CSF) virus†


RAE0242 WH211 E2 IgG1 CSF 1mg

RAE0826 WH303 E2 IgG1 CSF 1mg

Two monoclonals which detect the CSF virus specific antigenic domain on the majorenvelope glycoprotein of the virus. They bind to all strains of CSF virus and with noother pestiviruses. The monoclonals may be used for the specific identification of CSFvirus isolates as recommended by the EC. (See reference page 22).

Reference:Lin M, Lin F, Mallory M, Clavijo A (2000) J Virol 74, 11619 -11625.

†Not available in UK without prior approval of Defra.Please contact Product Sales for further details.

Infectious Bovine Rhinotracheitis


RAC0202 FITC Ig to Bovine Herpes virus 1 2ml PT

This antibody is raised in bovines. Intended for the in-vitro detection of specific viralantigens in frozen tissue sections, cytospin preparations and in cell cultures. Theconjugates are prepared by joining the immunoglobulin fraction of hyperimmuneantiserum to fluorescein isothiocyanate. Non-specific fluorescence is removed by gelfiltration and ion exchange chromatography. Freeze-dried presentation; store at 4°C.After reconstitution store at -20°C. Working dilution at least 1/10.

Please see page 22 for BVDV/BDV mAbs


2524

working togetherVLA and QIAGEN

VLA and QIAGEN are working together on a selected range of PCR-based, veterinary molecular assays.

These assays have been developed by VLA and are distributed byQIAGEN.

With a global network of sales professionalsserving customers in over 40 countries, QIAGENprovides you and your colleagues withstandardised solutions when you need them.High manufacturing standards, the QIAGENquality guarantee and expert technical serviceensure that you receive the best possibleresults from your research.

QIAGEN’s product expertise includes:

n Manual and automated technologies for DNA and RNA extraction and purification even from the most difficult sample types

n Optimised kits and protocols for PCR

n Rotor-Gene Q thermal cyclers

n cador real-time PCR assays for veterinary molecular diagnosticsl cador BVDV RT-PCR Kitsl cador BVDV Type1/2 RT-PCR Kitsl cador T.equigenitalis PCR Kits

For further information please contact QIAGEN UK LimitedQIAGEN House, Fleming Way, Crawley, West Sussex RH10 9NQ, UKTelephone +44 (0)1293 422911 Fax +44 (0)1293 422922 or visit:

www.qiagen.com

VLA Scientific and the JohnInnes Centre have createdTuff™RNA molecules that encodetarget sequences for a number ofRT-PCR assays for animal viruses.These molecules can be used as positivePCR or process controls for diagnosticmolecular tests, avoiding the risk of using aninfectious virus.

The Tuff™RNA is a genetically modified Cowpea Mosaic Virusthat is stable, transportable at 4oC and, most importantly is notinfectious to animals or plants. It can be incorporated into yourroutine testing schedule assuring the correct performance ofyour RT-PCR assay. As the target RNA is encapsidated, theproblem of RNA degradation associated with naked RNA orin-vitro transcripts is eliminated.

Tuff™RNAs available NOW:

Influenza A virus (M gene) RAK6270Bovine viral Diarrhoea virus (5‘UTR) RAK6271

Tuff™RNAs available later in 2010:

Rabies virusClassical swine fever virusPandemic H1N1 Influenza virusInfluenza A virus (H5, H7 and N1 targets)

Product not for sale in Australia and the United States.

Tuff™RNART-PCR Controls forRNA Viral Targets

VLA Scientific Product SalesNew Haw, Addlestone, Surrey KT15 3NB United KingdomTelephone +44 (0)1932 357641 Facsimile +44 (0)1932 357701Email: [email protected]: www.vlascientific.com

If you are interested in purchasing Tuff™RNAs please contact:

Bovine Prion Protein (PrP) Monoclonal Antibody

RAE5037 Bovine PrP R145/20/1/2/1 1mg PT

A monoclonal antibody from a mouse-rat heterohybridoma, prepared by the fusion ofrat splenocytes. Detects PrP in sheep and cattle, but not mouse or rat. Freeze driedpresentation, store at 4°C. After reconstitution store at -18°C or below.

Chick Embryo Cells

RAF3740 Chick Embryo Fibroblasts (CEF) 100ml

A suspension of fibroblast cells prepared from 9 -11 day old chick embryos seeded atleast 1x106 cells per ml. Store at 4°C.

Negative Control Serum

RAB0631 Negative Control Serum 0.5ml

Specific Pathogen Free (SPF) chicken serum prepared for use as a negative control inserological tests. Freeze-dried presentation; store at 4°C.


26


Miscellaneous Reagents

27

Maedi-Visna

Antisera

RAB0969 MV/CAE weak positive serum to gp-135 2ml PT

RAB0970 MV/CAE positive serum to gp-135 2ml PT

RAB0858 MV/CAE negative control serum 2ml PT

PLEASE NOTEThese antisera are suitable for use with the MAEDITECT diagnostic kit. One vial offreeze-dried reagent to be stored at 4ºC. Once reconstituted with 2ml of steriledistilled water, store at 4ºC for up to one month.

Diagnostic Kits

RAI0259 MAEDITECT ‘100’ PT

RAI0260 MAEDITECT ‘1000’ PT

Test kits for the serological diagnosis of Maedi-Visna (MV) in sheep by the Agar GelImmunodiffusion Test (AGIDT). Kits consist of precipitating antiserum to theglycosylated envelope polypeptide (gp 135) antigen of MV virus and MV antigen.MAEDITECT ‘100’ contains sufficient reagents for 100 tests and MAEDITECT ‘1000’for 1000 tests. Liquid presentation; stable for 1 year on storage at -20°C.

RAK0266 Agar gel cutter

RAI0267 Agar gel ‘100/30’

RAI0268 Agar gel ‘1000/300’

The ‘100/30’ agar gel pack contains sufficient gel for 100 tests and the ‘1000/300’ for1000 tests. Store at 4°C.

Parainfluenza 3 Virus


RAC0211 FITC Ig to Bovine Parainfluenza 3 virus 2ml PT


Respiratory Syncytial Virus


RAC0204 FITC Ig to Bovine Respiratory Syncytial virus 2ml PT



Veterinary Parasep®Faecal Filters

For fast and accurate Worm Egg CountsSingle use device for in-vitro diagnostic use only

www.woodleyequipment.com

l Improved health and safety -Separates eggs from faecal debris in a safe, enclosed environment.

l More consistent results - Simplified method reduces chance of error.

l Eliminates risk of cross contamination - Single use and disposable product.

l Proven to work with McMaster chamber techniques - Independent laboratory testing undertaken by a leading veterinary institution.

Validated by the Veterinary LaboratoriesAgency for repeatable and accurateWorm Egg Counts with Ovine, Bovineand Equine faecal samples.

Old Station Park Buildings, St Johns Street, Horwich, Bolton, Lancashire BL6 7NYTel: +44 (0)1204 669033 Fax: +44 (0)1204 669034 Email: [email protected]

2928

VLA Scientific offers acomplete diagnostic testing,advice and support service forthe diagnosis, control andprevention of disease infarm animals.

Tests carried out areassured by acomprehensivequality assurancesystem.Most tests are

subject to third partyaccreditation by UKAS.

For further information visit theVLA Scientific websitewww.vlascientific.com

testinglaboratoryVLA Scientific

services

For further information please contact:VLA ScientificVeterinary Laboratories AgencyNew Haw, Addlestone, Surrey KT15 3NBUnited Kingdom

Telephone +44 (0)1932 357641Facsimile +44 (0)1932 357701Email: [email protected]

Website: www.vlascientific.com

for massphenotyping andgenotypingPhengenix is a comprehensive cellcharacterisation service for prokaryoticand eukaryotic cells. It employs a set ofpowerful interrogative techniques thatdelivers support for a variety ofapplications including:

n Definitive cell description and characterisation

n Strain validationn Gene detection and identificationn Whole genome sequencingn Characterisation of probiotics (in-vitro& in-vivo)

n Development and evaluation of vaccinesn IP protection

Our scientists have expertise in genotyping andphenotyping methodologies and access toextensive data sources and internationalexpertise. They also have an in-depthknowledge of animal and zoonotic diseasesand a wide experience of food safety,environmental and public health issues.

Prof ic iency Test ing Schemes (Vetqas) Prof ic iency Test ing Schemes (Vetqas)

Antibody Detection

PT0001 African Horse Sickness (AHS) ELISA - serum

PT0137* African Swine Fever (ASF) ELISA - serum

PT0003* Aujeszky’s disease ELISA / SNT - serum/fluid

PT0005 Avian Influenza (AI) AGIDT / HAIT - serum

PT0142* Avian Leukosis Antigen ELISA - serum

PT0144* Avian Pneumovirus (APV) ELISA - serum

PT0145* Avian Reticuloendotheliosis (REV) ELISA - serum

PT0007 Babesia caballi IFAT / ELISA / CFT - serum

PT0009 Big Liver and Spleen disease (BLS) AGIDT - serum

PT0138* Bluetongue Virus (BTV) ELISA - serum

PT0011 Bovine Viral Diarrhoea (BVD) ELISA - milk

PT0012* Bovine Viral Diarrhoea (BVD) ELISA / SNT - serum

PT0015 Brucella abortus CFT & SAT - serum

PT0016 Brucella abortus ELISA - serum

PT0018 Brucella abortus ELISA - milk

PT0019 Brucella abortus Milk Ring test - milk

PT0020 Brucella abortus Rose Bengal test - serum

PT0022* Brucella canis SAT / RSA - serum

PT0035 Chlamydophila CFT / ELISA - serum

PT0128* Contagious Bovine Pleuropneumonia (CBPP) cELISA / CFT / IBT - serum

PT0036* Classical Swine Fever (CSF) ELISA / SNT - serum

PT0041 Egg Drop Syndrome (EDS) HAIT - serum

PT0044 Enzootic Bovine Leukosis (EBL) ELISA - milk

PT0045 Enzootic Bovine Leukosis (EBL) ELISA / AGIDT - serum

PT0046 Equine Infectious Anaemia (EIA) AGIDT - serum

PT0047 Equine Viral Arteritis (EVA) SNT - serum

PT0139* Foot & Mouth Disease (FMD)Eurasian Strains ELISA / VNT - serum

PT0065 Gamma interferon (Bovine TB) ELISA - simulated sera

PT0049 Glanders CFT - serum

PT0051* Hypoderma bovis (Warble fly) Titration / cELISA - serum

PT0054 IBR / PI3 / RSV ELISA - serum

PT0052 Infectious Bovine Rhinotracheitis (IBR) ELISA - milk

PT0053* Infectious Bovine Rhinotracheitis (IBR) ELISA / SNT - serum

PT0055 Infectious Bronchitis Virus (IBV) avian HAIT - serum

PT0056 Infectious Bursal Disease (IBD) Gumboro AGIDT - serum

PT0058* Leptospira hardjo bovis MAT - serum

PT0057 Leptospira hardjo ELISA - milk

PT0059 Maedi visna / CAEV AGIDT / ELISA - serum

PT0068 Mycobacterium paratuberculosis - Bovine ELISA - serum

PT0120* Mycoplasma gallisepticum - Chicken RSA / ELISA - serum

PT0122* Mycoplasma gallisepticum &meleagridis - Turkey RSA / ELISA - serum

PT0072 Mycoplasma hyopneumoniae ELISA - serum

PT0155* Neospora caninum ELISA / IFAT - serum

PT0076 Porcine Parvovirus (PPV) ELISA / HAIT - serum

PT Schemes

Test Method (Currently in use)

PT Schemes


PT0077 Porcine Reproductive &Respiratory Syndrome (PRRS) ELISA / IPMA - serum

PT0083 Salmonella meat juice ELISA - serum

PT0106 Theileria (Babesia) equi IFAT / CFT / ELISA - serum

PT0110 Toxoplasma gondii LAT / IFAT - serum

PT0112 Trypanosoma equiperdum (Dourine) IFAT / CFT - serum

Haematology

PT0050 Haematology - ruminant blood Blood cell counts

Clinical Chemistry

PT0107* Tissue - copper Tissue biochemistry

PT0108* Tissue - lead Tissue biochemistry

Transmissible Spongiform Encephalopathy

PT0124*✝ BSE Rapid Tissue Test Rapid BSE tests - brain

PT0135 PrP Immunohistochemistry Technical Histopathology - brain sections

PT0094 Scrapie blood genotyping Genotyping - blood

PT0125*✝ Scrapie Rapid Tissue Test Rapid Scrapie tests - brain

Virology

PT0010 Bovine Viral Diarrhoea (BVD) virus AgELISA / PCR - blood

PT0031 IBR / RSV / PI3 Fluorescent antibody tests

Parasitology

PT0111 Trichinella spiralis Enumerationin post digestion filtrate

PT0114* Worm / Fluke egg WEC / FEC faeces

Feed Tests

PT0063 Bone in animal feed Microscopic examination

PT0080 Protein in animal feed PCR

Microbiology

PT0002 Bacillus anthracis Microscopic examination of blood

PT0008 Bacterial diseases in cold water fish Isolation & ID of fishpathogens

PT0025 Brucella abortus - slides Stain & ID

PT0039 E.coli 0157 Isolation & ID from clinicalsamples

PT0040 E.coli Serotyping

PT0134 Equine microbiology Isolation & ID of equine pathogens

PT0060 Mastitis - bovine Isolation & ID of milkpathogens

PT0061* Microbiology - farm animals Isolation & ID of animalpathogens

PT0127* Mycobacterium avian subsp.paratuberculosis (Johnes) Culture - faeces

PT0154* Mycobacterium avian subsp.paratuberculosis (Johnes) PCR - faeces

PT0121* Mycoplasma gallisepticum &meleagridis culture Isolation & ID

PT0126 Mycoplasma hyopneumoniae Culture

PT0150 Mycoplasma hyopneumoniae PCR

PT0074* Pasteurella multocida toxin detection Culture & ELISA / PCR

3130

To order: Contact: VLA Quality Assurance Unit (Vetqas) Tel +44 (0)1509 670607 Fax +44 (0)1509 670206 or Email: [email protected] visit: www.vla.gov.uk/eqa/eqa-home.htm

3332

VLA Worldwide Distributors

Algeria, Bahrain, Cyprus, Egypt,Ethiopia, India, Iran, Iraq, Jordan,Kuwait, Lebanon, Libya, Nepal, Oman,Pakistan, Qatar, Saudi Arabia, Sudan,Syria, Turkey, UAE, Yemen.

Lillidale Animal Health LimitedBadbury View, Bothenwood, Wimborne,Dorset BH21 4HU, UK

� +44 (0)1202 848456 � +44 (0)1202 848570� [email protected]� www.lillidale.co.uk

Products - All Products

Brazil (and other South Americacountries)

HIPRA Brazil LtdaAv. Cristóvão Colombo, 2360 - Ap 60290560-002Porto Alegre - (RS), Brazil

� +55 (51) 3325-4500� [email protected]� www.hipra.com.br


Croatia & Slovenia

Labormed d.o.o.Periceva 29, 1000 Ljubljana, Slovenia

� +386 1 4364901� +386 1 4364905� [email protected]� www.labormed.si


Germany & Austria

C.C.Pro GmbHAm Bahnhof 1, D-99986, OberdorlaGermany

� +49 700 2277 6366� +49 700 2277 6329� www.c-c-pro.com


Israel

NBT New Biotechnology Limited3 Mekor Haim Street, Jerusalem 91086Israel

� +972 2 6732001� +972 2 6731611� [email protected]� www.nbtltd.com


Italy

Star Ecotronics SRLVia Ascanio Sforza 87, 20141, MilanItaly

� +39 (0)2 89540225� +39 (0)2 89549300� [email protected]� www.starecotronics.it


Romania

SC Redox Lab SRL.Str. Maramuresului 38, Cluj-NapocaCluj, Romania

� +40 264 416427� +40 264 439884� [email protected]� www.redoxcj.ro


Spain & Portugal

Genomica S.A.U.Alcarria, 7- Pol. Industrial de CosladaCoslada 28820, Madrid, Spain

� +34 91 674 8990� +34 91 674 8991� [email protected]� www.genomica.es


Worldwide - unless covered by otherdistributors

Pro-Lab DiagnosticsUnit 7, Westwood CourtClay Hill Industrial Estate, NestonSouth Wirral, Cheshire CH64 3UJUnited Kingdom

� +44 (0)151 3531613� +44 (0)151 3531614� [email protected]� www.pro-lab.com

Products - Salmonella Antisera

Microbiology (continued)

PT0087 Salmonella in animal feed Isolation & ID

PT0088 Salmonella in poultry Isolation & ID

PT0084 Salmonella serotyping Serotyping

PT0026* Staphylococci - including MRSA Culture & ID - sim. clinical specimen

Non UK laboratoriesPT0102 Taylorella equigenitalis only Isolation & ID

Non UK laboratoriesPT0104 Taylorella / Klebsiella / Pseudomonas Isolation & ID

Prof ic iency Test ing Schemes

PT Schemes


*New or updated schemes

✝ VLA is the EU Community Reference Laboratory for TSEs. The schemes are separate from, and do not replace, those run by the Community Reference Laboratory for participation by the National Reference Laboratories within the EU.

The Quality Assurance Unit is always looking to increase the range of PT Schemes, soplease visit our website for any new Schemes:

www.vla.gov.uk/eqa/eqa-home.htm

3534

CCAMPYCULTURE - Campylobacter fetus spp RAI0800 10

CapriLAT RAI6224 14

Chick embryo fibroblasts (CEF) RAF3740 27

Classical swine fever (CSFV) mAb RAE0242, RAE0826 23

Clostridium perfringens � toxin monoclonal antibody RAE0234 10

COMPELISA 160 - Brucella RAI2005 9

COMPELISA 400 - Brucella RAI2006 9

Contagious bovine pleuropneumonia (CBPP) RAI6223 14

Contagious caprine pleuropneumonia (CCPP) RAI6224 14

EE.coli monoclonal antibodies to fimbrial adhesins K88a RAE0235 10

K88b RAE0236 10

987P RAE0237 10

F41 RAE0238 10

K99 RAE0239 10

E.coli polyclonal antisera See page 10/11

Egg drop syndrome '76 antigen RAA0081 17

Egg drop syndrome '76 positive serum RAB0082 17

FFIMBREX K99 - E.coli RAI0261 11

FIMBREX F41 - E.coli RAI0262 11

FIMBREX K88 - E.coli RAI0263 11

FIMBREX 987P - E.coli RAI0264 11

IIBRV conjugated antibody FITC-Ig to bovine herpes virus 1 RAC0202 23

Identibac bacterial genotyping See pages 12

Infectious bronchitis virus CVL793/B antigen RAA2003 17

Infectious bronchitis virus CVL793/B positive serum RAB2004 17

Infectious bronchitis virus D274 antigen RAA0715 17

Infectious bronchitis virus D1446 antigen RAA0716 17

Infectious bronchitis virus M41 antigen RAA0121 17

Infectious bronchitis virus M41 positive serum RAB0122 17

Infectious bronchitis virus 755/03 antigen RAA5041 17

Infectious bronchitis virus 755/03 positive serum RAB5040 17

Infectious bursal disease (Gumboro disease) virus antigen RAA0123 21

Infectious bursal disease virus positive serum RAB0124 21

Catalogue No(s). Page

Index

AAgar gel cutter RAK0266 26

Agar gel ‘100/30’ RAI0267 26

Agar gel ‘1000/30’ RAI0268 26

Antimicrobial testing See page 15

Applied Biosystems avian influenza detection kits See page 20

Avian adenovirus (CELO) antigen RAA0026 16

Avian adenovirus (CELO) positive serum RAB0027 16

Avian influenza antigens and antisera See page 18/19

Avian paramyxovirus (PMV 3) positive serum RAB0033 16

Avian reovirus antigen RAA0028 16

Avian reovirus positive serum RAB0029 16

BBacterial isolates See page 15

BoviLAT RAI6223 14

Bovine PrP mAb RAE5037 27

Brucella abortus/melitensis/suis CFT antigen RAA0066 8

Brucella abortus MRT antigen RAA0048 8

Brucella abortus SAT antigen RAA0054 8

Brucella abortus negative serum RAB0701 9

Brucella abortus positive serum RAB1003 9

Brucella melitensis positive serum RAB0974 9

Brucella ovis negative serum RAB0075 9

Brucella RBT (Standard) antigen RAA0060 8

Brucella RBT (Sensitive) antigen RAA2016 8

BRUCELISA 160, 160SG RAI0990, RAI2017 9

BRUCELISA 400, 400SG RAI0705, RAI2018 9

BRUCELISA 160M RAI0991 9

BVDV conjugated antibody to bovine viral diarrhoeaRAC0205 23

BVDV, BDV monoclonal antibodies See page 22

BVDV, BDV, pan-pestivirus reactive monoclonal antibodies See page 22

BVDV positive serum RAB0042 23


36

Index

MMAEDITECT '100' - MV RAI0259 26

MAEDITECT '1000' - MV RAI0260 26

Marek’s disease virus antigen RAA0156 21

Marek's disease virus positive serum RAB0157 21

MV/CAE negative control serum RAB0858 26

MV/CAE positive serum to gp-135 RAB0970 26

MV/CAE weak positive serum to gp-135 RAB0969 26

Mycobacterium bovis RAI5047 14

Mycoplasma latex reagent kits RAI6223/RAI6224 14

NNegative control serum – chicken RAB0631 27

Newcastle disease virus antigen RAA0158 21

Newcastle disease virus positive serum RAB0155 21

PPhengenix phenotyping and genotyping service See page 28

PI3V conjugated antibody FITC-Ig to bovineparainfluenza 3 virus RAC0211 26

Proficiency testing schemes (Vetqas) 30/32

Pro-Lab Diagnostics See page 13

QQiagen sample and assay technologies See page 24

RRespiratory syncytial virus FITC Ig to Bovine RSV3 RAC0204 26

SSalmonella agglutination typing antisera 13

Salmonella Pullorum stained antigen RAA0191 14

TTrichomoniasis RAI0800 10

Tuff TMRNA Influenza A virus (M gene) RAK6270 25

Tuff TMRNA Bovine viral diarrhoea virus (5‘UTR) RAK6271 25

VVeterinary Parasep® Faecal filters 27

VLA Scientific 2/3

VLA Scientific biological reagents 4

VLA general information 6/7

VLA Scientific testing services 29

VLA Scientific proficiency testing (Vetqas) 5

VLA worldwide distributors 33


VLA is an Executive Agency of the Department forEnvironment, Food and Rural Affairs (Defra) VLAS5/04.2010/1

Safeguarding public and animal health

To contact VLA Scientific:Telephone: +44 (0)1932 357641 Facsimile: +44 (0)1932 357701Email: [email protected]

VLA Weybridge, New Haw, Addlestone, Surrey KT15 3NB United Kingdom

www.vlascientific.com Biological Reagents &Proficiency Testing

Catalogue 2010 11


The Veterinary Laboratories Agency is a UKnetwork of veterinary laboratories and isinternationally recognised as a centre of scientificexcellence.VLA safeguards public and animal health byproviding all sectors of the animal health industrywith veterinary research, diagnostic services andanimal disease surveillance of farmed livestock andwildlife.


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