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Volume 66 May 1970 Number 1

Proceedings of the

National Academy of Sciences

of the United States of America

OF***a*****

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Proceedings of the

National Academy of Sciences

of the United States of America

VOLUME 66 MAY-AUGUST 1970

f @~~~~1o

<,~ X

EDITORIAL BOARD OF THE PROCEEDINGS

JOHN T. EDSALL, Chairman WALTER KAUZMANN, Vice Chairman MERLE A. TUVE, Home Secretary

JAMEs V. NzEL, Vice Chairman HARRISON S. BROWN, Foreign Secretary

E. R. PioRE, Treasurer

C. B. ANFINSEN FRANK BRINK, JR. R. KEITH CANNAN BERNARD D. DAVIS KATHERINE ESAU

WILLIAM A. FOWLER LEO GOLDBERG GORDON J. F MACDONALD ELLIOTT W. MONTROLL

THEODORE T. PUCK RICHARD B. ROBERTS H. L. SHAPIRO EMIL L. SMITH JOSEPH L. WALSH

JOSEPHINE A. WILLIAMS, Editorial A8sociate

PUBLISHED MONTHLY BY THE NATIONAL ACADEMY OF SCIENCES



T'he Pr oceedings of the National Academy of Sciences

OFFICERS OF THE ACADEMY

PHILIP HANDLER President

G. B. KISTIAKOWSKY Vice President

MERLE A. TUVE Home Secretary

HARRISON S. BROWN Foreign Secretary

E. R. PIORE Treasurer

EDITORIAL BOARD OF THE PROCEEDINGS

JOHN T. EDSALL, Chaimnian

WALTER KAUZMANN, Vice Chairman

MERLE A. TUVE, Home Secretary

JAMES V. NEEL, Vice Chairman

HARRISON S. BROWN, Foreign Secretary

E. R. PIORE, Treasurer

C. B. ANFINSEN FRANK BRINK, JR. R. IKEITH CANNAN BERNARD D. DAVIS KATHERINE ESAU

WILLIAM A. FOWLER LEO GOLDBERG GORDON J. F. IMACDONALD ELLIOTT W. MONTROLL

THEODORE T. PUCK RICHARD B. ROBERTS H. L. SHAPIRO EMIL L. SMITH JOSEPH L. WALSH

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TiE 1PROCEEDINGS OF THIE NATIONAL ACADEMY OF SCIENCES,

is published monthly by THE NATIONAL ACADEMY OF SCIENCES



CONTENTS

NATIONAL ACADEMY OF SCIENCES

NATIONAL ACADEMY OF SCIENCES: OFFICERS, COUNCIL, AND MEMBERS. 1307 INFORMATION TO CONTRIBUTORS TO THE PROCEEDINGS OF THE. NATIONAL ACADEMY OF

SCIENCES. ANNUAL MEETING.-SCIENTIFIC PROGRAM AND ABSTRACTS OF CqNTRIBUTED PAPERS . 221 INDEX TO AUTHORS OF ABSTRACTS. 247 INDEX TO AuTHORS OF ARTICLES (Volume 66) .1351 SUBJECT INDEX (Volume 66) .1355

PHYSICAL SCIENCES

ASTRONOMY

INTERPRFTATION OF THE [CIIII] LINES IN GASEOUS NEBULA-. . By L. H. Aller, S. J. Czyzak, M. F. Walker, and P. K. Krueger 1

EXCITATION OF METASTABLE LEVELS IN Low DENSITY NEBULAR 'LASMAS: [SII] AND [ARIVI. By S. J. Czyzak, T. K. Krueger, and L. H. Aller 282

THE SOLAR ABUNDANCE OF GALLIUM . . . . By John Ross and Lawrence Aller 983

CHEMISTRY

ACTIVE SITE OF a-CHYMOTRYPSIN ACTIVATION BY ASSOCIATION-DESOLYVIa6N . By Saul G. Cohen, Vishnu M. Vaidya, antd Ricfard M. Schultz 249

IS THE BREAKDOWN OF THE BORN-OPPENHEIMER APPROXIKATION RESPONSIBLE FOR INTERNAL CONVERSION IN LARGE MOLECULES?.

.By D. M. Burland and 9. W. Robinson 257

MATHEMATICS

SINGULAR INTEGRALS AND THE PRINCIPAI SERIES, II. .By A. W. Knapp qnd E. M. Stein 13

CHARACTERISTIC CLASSES AND WEYL TENSOR: APPLICATIONS TO GENERAL RELATIVITY .L?y Andr6 Avez 265

A SINGULAR ABSTRACT CAUCHY PROBLEM. By J. A. Donaldson 269 THEORY OF A CLASS OF LOCALLY CONVLX VECTOR LATTICES WIUCH INCLUDE THE

LEBESGUE SPACES .By Witold M. Bogdanowicz 275 FLOWS WITH CROSS SECTIONS .By A lberto Verjovsky 1154

APPLIED MATHEMATICAL SCIENCES

STATISTICAL THEORIES OF SUCCESS. By Clarence Zener 18

PHYSICS

SEARCH FOR POSITRONIUM LYMAN a-RADIATION FROM POSITRONS STOPPED IN INERT GASES. BY Marvin Leventhal 6

PHYSICAL PARTJCLE REPRESENTATION AND GENERALIZED TRANSFORMATION THEORY . By I. Prigogine, Cl. George, F. Henin, P. Mandell, and J. W. Turner 709

iii



iv CONTENTS

BIOLOGICAL SCIENCES

BIOCHEMISTRY

MECHANISM OF ACTIVATION OF CATABOLITE-SENSITIVE GENES: A POSITIVE CONTROL

SYSTEM. By Geoffrey Zubay, Daniele Schwartz, and Jon Beckwith 104 SECONDARY STRUCTURE OF THE CYCLIC MOIETY OF THE PEPTIDE HORMONE OXYTOCIN

AND ITS DEAMINO ANALOG . By D. W. Urry, M. Ohnishi, and Roderich Walter 111 THE MITOCHONDRIAL RIBOSOME OF Xenopus laevis.

.By Ronald F. Swanson and Igor B. Dawid 117 TOPOLOGICAL AND FUNCTIONAL ORGANTZATION OF THE MITOCHONDRION.

. By Jennie M. Smoly, Bo Kuylenstierna, and Lars Ernster 125 THE DYNAMICS OF LIFE, I. DEATH FROM INTERNAL IRRADIATION BY 239PU AND 226RA,

AGING, CANCER, AND OTHER DISEASES . . By Betsy J. Stover and Henry Eyring 132 IDENTITY OF THE 5'-TERMINAL RNA NUCLEOTIDE SEQUENCE OF THE SATELLITE To-

BACCO NECROSIS VIRES AND ITS HELPER VIRUS: POSSIBLE ROLE OF THE 5'-TER-

MINUS IN THE RECOGNITION BY VIRUS-SPECIFIC RNA REPLICASE

.By J. A. Lesnaw and M. E. Reichmann 140 BINDING OF ADENOSINE 3': 5'-CYCLIC PHOSPHATE TO G FACTOR OF Escherichia coli, AND

ITS EFFECTS ON GTPASE, RNASE V, AND PROTEIN SYNTHESIS

.By Michihiho Kuwano and David Schlessinger 146 DOLICHOL MONOPHOSPHATE GLUCOSE: AN INTERMEDIATE IN GLUCOSE TRANSFER IN

LIVER. By Nicolds H. Behrens and Luis F. Leloir 153 REGULATION OF AxON FORMATION BY CLONAL LINES OF A NEURAL TUMOR .

. By N. W. Seeds, A. G. Gilman, T. Amano, and M. W. Nirenberg 160 RELEASE OF TRNA FROM RIBOSOMES BY A FACTOR OTHER THAN G FACTOR

. . . . . . . . By Hieo Ishitsuka and Akira Kaji 168 CYCLIC CHANGES IN THYLAKOID MEMBRANES OF SYNCHRONIZED Chlamydomonas rein-

hardi .By Seth Schor, Philip Siekevitz, and George E. Palade 174 TRANSCRIPTIONS OF THE BACTERIOPHAGE T4 TEMPLATE In Vitro: SEPARATION OF

"DELAYED EARLY" FROM "IMMEDIATE EARLY" TRANSCRIPTION.

By Gabriele Milanesi, Edward N. Brody, Oscar Grau, and E. Peter Geiduschek 181 COOPERATIVE EFFECTS IN MODELS OF STEADY-STATE TRANSPORT ACROSS MEMBRANES,

II. OSCILLATING PHASE- TRANSITION . . . By Terrell L. Hill and Yi-der Chen 189 COMPLEMENTARITY OF RNA PRODUCED BY ENZYMIC TRANSCRIPTION OF NATIVE AND

DENATURED B. subtilis DNA . By Era Cassuto, Marshall Stein, and Erwin Chargaff 197 (ERRATUM, 62, 558).-THE ROLES OF SYNTHESIS AND DEGRADATION IN DETERMINING

TISSUE CONCENTRATIONS OF LACTATE DEHYDROGENASE-5

. By Paul J. Fritz, Elliot S. Vesell, E. Lucile White, and Kenneth M. Pruitt 218 (ERRATUM, 64, 1103).-PHOTOREGULATION OF BIOLOGICAL ACTIVITY BY PHOTOCHROMIC

REAGENTS, II. INHIBITORS OF ACETYLCHOLINESTERASE

By Joseph Bieth, Spyros M. Vratsanos, Norbert Wassermann, and Bernard F. Erlanger 218 POSTTRANSCRIPTIONAL CONTROL OF TYROSINE AMINOTRANSFERASE SYNTHESIS BY

INSULIN. By Thomas D. Gelehrter and Gordon M. Tomkins 390 ELEVATION OF GUANOSINE 3',5'-CYCLIC PHOSPHATE IN RAT HEART AFTER PERFUSION

WITH ACETYLCHOLINE. . By W. J. George, J. B. Polson, A. G. O'Toole, and N. D. Goldberg 398

Bacillus s.ubtilis MUTANT ALTERED IN SPORE MORPHOLOGY AND IN RNA POLYMERASE ACTIVITY . By Roy H. Doi, Lyle R. Brown, Gregory Rodgers, and Yao-pi Hsu 404

ELECTROPHORETICALLY HOMOGENEOUS ANTI-DNP ANTIBODIES WITH RESTRICTED ISO-

ELECTRIC POINTS ELICITED IN MICE BY IMMUNIZATION WITH THE ANTIGEN PAPAIN-

S-DNPL .By Gary N.- Trump and S. J. Singer 411 ELECTRON MICROSCOPY OF KINETOPLASTIC DNA FROM Trypanosoma mega .

.By Monique Laurent and M. Steinert 419



CONTENTS v

SYNTHESIS OF IMMUNOGLOBULIN HEAVY AND LIGHT CHAINS BY THE FREE RIBOSOMES OF

A MOUSE PLASMA CELL TUMOR . By B. Lisowska-Bernstein, M. E. Lamm, and P. Vassalli 425

MEMBRANE STRUCTURE: LIPID-PROTEIN INTERACTIONS IN MICROSOMAL MEMBRANES

. By Benjamin F. Trump, Sue M. Duttera, William L. Byrne, and Antii U. Arstila 433 THE DYNAMICS OF LIFE, II. THE STEADY-STATE THEORY OF MUTATION RATES

.By Henry Eyring and Betsy J. Stover 441 A SOURCE FOR THE SPECIAL CATALYTIC POWER OF ENZYMES: ORBITAL STEERING .

.By Dan R. Storm and D. E. Koshland, Jr. 445 NEURALLY MEDIATED INCREASE IN DOPAMINE-_,HYDROXYLASE ACTIVITY .

By Perry B. Molinoff, Steven Brimijoin, Richard Weinshilboum, and Julius Axe7rod 453 INTERCONVERTIBLE FORMS OF GLYCOGEN PHOSPHORYLASE IN Neurospora crassa .

.By Maria T. Tgllez-Iiion and Hictor N. Torres 459 RAPID PURIFICATION AND THE IMMUNOLOGICAL SPECIFICITY OF MAMMALIAN MICRO-

TUBULAR PARACRYSTALS POSSESSING AN ATPASE ACTIVITY

.By Ariaki Nagayama and Samuel Dales 464 ISOLATION, CHARACTERIZATION, AND LOCATION OF A DONOR-ACCEPTOR UNIT FROM

CROSS-LINKED FIBRIN. By Renne Chen and Russell F. Doolittle 472 CYCLIC AMP RECEPTOR PROTEIN OF E. coli: ITS ROLE IN THE SYNTHESIS OF INDUCIBLE

ENZYMES

. By Michael Emmer, Benoit deCrombrugghe, Ira Pastan, and Robert Perlman 480 CODING AND CONFORMATIONAL PROPERTIES OF OLIGONUCLEOTIDES MODIFIED WITH THE

CARCINOGEN N-2-ACETYLAMINOFLUORENE. By Dezider Grunberger, James H. Nelson, Charles R. Cantor, and I. Bernard Weinstein 488

SYNTHESIS OF ACID MUCOPOLYSACCHARIDES BY GLIAL TUMOR CELLS IN TISSUE CULTURE

.By Albert Dorfman and Pei-Lee Ho 495 IDEhNTIFICATION OF L-METHIONINE-S-SULFOXIMINE AS THE CONVULSANT ISOMER OF

METHIONINE SULFOXIMINE. By W. Bruce Rowe and Alton Meister 500 STRUCTURE AND FUNCTION OF E. coli FORMYLMETHIONYL TRNA, I. EFFECT OF MODI-

FICATION OF PYRIMIDINE RESIDUES ON AMINOACYL SYNTHETASE RECOGNITION

.By LaDonne H. Schulman 507 GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND Neurospora MORPHOLOGY .

.By W. A. Scott and E. L. Tatum 515 STUDIES ON THE FORMATION OF TRANSFE.R RIBONUCLEIC ACID-RIBOSOME COMPLEXES,

XII. PHENYLAIANYLOLIGONUCLEOTIDE BINDING TO E. coli RIBOSOMES: NECES- SITY FOR A FREE AMINO GROUP

. By Tokutaro Hishizawa, James L. Lessard, and Sidney Pestka 523 13-DESMETHYL RHODOPSIN AND 13-DESMETHYL ISORHODOPSIN: VISUAL PIGMENT

ANALOGUES. By Ralph Nelson, J. Kim deRiel, and Allen Kropf 531 Qg-REPLICASE AND E. coli TRANSCRIPTASE: REQUIREMENTS FOR SUBSTRATE SELECTION

AS REVEALED BY A STUDY OF BASE ANALOGS

.By A. M. Kapuler and S. Spiegelman 539 STUDIES OF THE MFCHANISM OF ANTHRANILATE; SYNTHASE REACTION.

.By H. Tamir and P. R. Srinivasan 547 EFFECT OF LACTATE ON COLLAGEN PROLINE HYDROXYLASE ACTIVITY IN CULTURED

L-929 FIBROBLASTS . . . . By John Paul Comstock and Sidney Udenfriend 552 IMPLICATIONS OF HEAVY CHAIN DISEASE PROTEIN SEQUENCES FOR MULTIPLE GENE

THEORIES OF IMMUNOGLOBULIN SYNTHESIS . By William D. Terry and Jack Ohms 5,58 ABTILITY OF NONENZYMIC NITRATION OR ACETYLATION OF E. coli GLUTAMINE SYNTHE-

TASE TO PRODUCE EFFECTS ANALOGOUS TO ENZYMIC ADENYLYLATION .

. By Filiberto Cimino, Wayne B. Anderson, and E. R. Stadtman 564 RIBONUCLEiC ACID SYNTHESIS OF VESICULAR STOMATITIS VIRUS, II. AN RNA POLY-

MERASE IN THE VIRION. . By David Baltimore, Alice S. Huang, and Martha Stampfer 572



vi CONTENTS

DINUCLEOTIDES AS PRODUCTS OF AN EXONUCLEOLYTIC ACTIVITY ASSOCIATED WITH THE

MicrOcoccus luteus DNA POLYMERASE

By S. J. Harwood, P. F. Schendel, L. K. Mliller, and R. D. Wells 595 COO'ERATIVE EFFECTS IN MODELS OF STEADY-STATE TRANSPORT ACROSS MEMBRANES,

III. SIMULATION OF POTASSIUM ION TRANSPORT IN NERVE.

.By Terrell L. Hill and Yi-der Chen 607 BIOLOGICAL MEMBRANE STRUCTURE, I. THE PROTEIN CRYSTAL MODEL FOR MEMBRANES

.By Garret Vanderkooi and David E. Green 615 PHOSPHOGLUCOMUTASE MUTANTS OF Neutrospira sitophola AND THEIR RELATION TO

MORPHOLOGY. By N. C. Mishra and E. L. Tatum 638 REPLICATIVE FOiM II DNA OF 4X174; RESISTANCE TO EXONUCLEOLYTIC CLEAVAGE

BY E. coli DNA POLYMERASE . . By Aharon Razin and Robert L. Sinsheimer 646 USE OF DIMETHYL SUBERIMIDATE, A CROSS-LINKING REAGENT, IN STUDYING THE SUB-

trNIT STRUCTURE OF OLIGOMERIC PROTEINS

.By Gregg E. Davies and George R. Stark 651 THE DYNAMICS OF LIFE, III. MECHANISMS OF NONSURVIVAL AND THE RELATION OF

DOSE SIZE . By Betsy J. Stover and Henry Eyring 672 FORMATION OF A VACCINIA VIRUS STRUCTURAL POLYPEPTIDE FROM A HIGHER MOLECU-

LAR WEIGHT PRECURSOR: INHIBITION BY RIFAMPICIN

.By Ehud Katz and Bernard Moss 677 ROLE OF RNA SYNTHESIS IN THE ESTROGEN INDUCTION OF A SPECIFIC UTERINE PROTEIN

.By Anthony B. DeAngelo and Jack Gorski 693 INITIATION AND RELEASE OF RNA BY DNA-DEPENDENT RNAiPOLYMERASE

.By Robert L. Millette and Carol D. Trotter 701 A T4-INDUCED ENDONUCLEASE WHICH ATTACKS T4 DNA

.By Sidney Altman and Matthew Meselson 716 ELECTRON PARAMAGNETIC RESONANCE STUDIES OF SPIN-LABELED HEMOGLOBINS AND

THEIR IMPLICATIONS TO THE NATURF OF COOPERATIVE OXYGEN BINDING TO HEMO-

GLOBIN .By Chien Ho, Joseph J. Baldassare, and Samuel Charache 722 PROTIINS SPECIFIED BY HERPES SIMPLEX VIRUS, IV. SITE OF GLYCOSYLATION AND

ACCUMULATION OF VIRAL MEMBRANE PROTEINS

.By Patricia G. Spear and Bernard Roizman 730 SUBU'NIT STRUCTURE OF HUMAN FIBRINOGEN, SOLUBLE FIBRIN, AND CROSS-LINKED

INSOLUBLE FIBRIN . . By Patrick A. McKee, Patrick MIattock, and Robert L. Hill 738 CONTROLLED DEAMIDATION OF PEPTIDES AND PROTEINS: AN EXPERIMENTAL HAZARD

AND A POSSIBLE BIOLOGICAL TIMER. . By Arthur B. Robinson, James H. McKerrow, and Paul Cary 753

TEMPORAL RESOLUTION OF INDIVIDUAL STEPS IN AN ENZYMIC REACTION AT Low TEM-

PE,RATURE. By P. Douzou, R. Sireix, and F. Travers 787 OPTICAL ACTIVITY OF BIOLOGICAL MEMBRANES: SCATTERING EFFECTS AND PROTEIN

CONFORMATION . By Allan S. Schneider, Mary-Jane T. Schneider, and K. Rosenheck 793

PRECITPITATION OF PROTEINS BY VIRBLASTINE AND CALCIUM IONS.

.By L. Wilson, J. Bryan, A. Ruby, arid D. Mazia 807 CONFORMATIONAL SIMILARITY OF OVINE PROLACTIN AND BOVINE GROWTH HORMONE .

.By S. AM. Aloj and H. Edelhoch 830 BIOLOGICAL ELECTRON TRANSPORT SYSTEMS.

By Dwaine 0. Cowan, Gavril Pasternak, and Frank Kaufman 837 CONVERSION OF BACTERIAL ALDOLASE FROM VEGETATIVE TO SPORE FORM BY A SPORULA-

TION-SPECIFIC PROTEASE.

.By H. L. Sadoff, Emel Celikkol, and H. L. Engelbrech.t 844 PHOTOREGULATION OF BIOLOGICAL ACTIVITY BY PHOTOCHROMIC REAGENTS, IV. A

M9OEL FOR DIURNAL VARIATION OF ENZYMIC ACTIVITY. By Joseph Bieth, Norbert Wassermann, Spyros M. Vratsanos, and Bernard F. Erlanger 850



CONTENTS vii

TRANSLATIONAL COMPLEMENTARITY OF RNA TRANSCRIPTS OF NATIVE AND DENATURED

B. subtilis DNA. By Era Cassuto and Erwin Chargaff 863 DIRECTIONAL CHARACTER OF PROTON TRANSFER IN ENZYME CATALYSIS.

.By Jui H. WVang 874 PROPERTIES OF JINA TRANSCRIPTASE IN RFOViRUS SUBVIRAL PARTICLES .

.By Daniel H. Levin, Naomi Mendelsohn, Michael Schonberg, Hanna Klett, Samuel Silverstein, Alan Al. Kapuler, and George Acs 890

A NEW APPROACH TO ISOELECTRIC FOCUSING AND FRACTIONATION OF PROTEINS IN A PH

GRADIENT. By Stephen J. Luner and Alexander Kolin 898 MEMBRANE STRUCTURE: SPIN LABELING AND FREEZE ETCHING OF Mycoplasma laidlawii

. By Mark E. Tourtellotte, Daniel Branton, and Alec Keith gog LOCALIZATION OF CYTOCHROME IN INTACT AND FRAGMENTED MITOCHONDRIAL MEM-

BRANES. By Britton Chance, M. Erecifiska, and C. P. Lee 928 IMMUNOGLOBULIN SYNTHESIS AND SECRETION, IV. SITES OF INCORPORATION OF SUGARS

AS DETERMINED BY SUBCELLULAR FRACTIONATION

.By Jonathan W. Uhr and Isaac Schenkein 952 ISOLATION AND CHARACTERIZATION OF STRUCTURALLY HOMOGENEOUS ANTIBODIES FROM

ANTIPNEUMOCOCCAL SERA .

By Jean-Claude Jaton, Michael D. Waterfield, Michael N. Margolies, and Edgar Haber 959 Two FORMS OF YEAST GLYCOGEN SYNTHETASE AND THEIR ROLE IN GLYCOGEN ACCUMU-

LATION. By Lucia B. Rothman-Denes and Enrico Cabib 967 ATTACHMENT OF CARBOHYDRATE TO THE VARIABLE REGION OF MYELOMA IMMUNO-

GLOBULIN LIGHT CHAINS. By Harold C. Sox, Jr., and Leroy Hood 975 SYNTHESIS AND SECRETION OF -y-GLOBULIN BY LYMPH NODE CELLS, VIII. ORDER OF

SYNTHESIS OF THE INTERCHAIN DISULFIDE LINKAGES OF IMMUNOGLOBULINS . . By Earl W. Sutherland III, Daniel H. Zimmerman, and Milton Kern 987

GLUCOCORTICOID REGULATION OF ACTH SENSITIVITY OF ADENYL CYCLASE IN RAT FAT

CELL MEMBRANES. By T. Braun and 0. Hechter 995 BINDING OF DODECYL SULFATE TO PROTEINS AT HIGH BINDING RATIOS. POSSIBLE

IMPLICATIONS FOR THE STATE OF PROTEINS IN BIOLOGICAL MEMBRANES

.By Jacqueline A. Reynolds and Charles Tanford 1002 THE RELATIONSHIP OF A MEMBRANE-BOUND D-(-)-LACTIC DEHYDROGENASE TO AMINO

ACID TRANSPORT IN ISOLATED BACTERIAL MEMBRANE PREPARATIONS .

.By H. R. Kaback and L. S. Milner 1008 In Vitro ASSEMBLY OF Neurospora ASSIMILATORY NITRATE REDUCTASE FROM PROTEIN

SUBUNITS OF A Neurospora MUTANT AND THE XANTHINE OXIDIZING OR ALDEHYDFE

OXIDASE SYSTEMS OF HIGHER ANIMALS. By Paul A. Ketchum, Helene Y. Cambier, William A. Frazier III, Charles Madansky, and Alvin Nason 1016

COMPONENTS OF HISTIDINE TRANSPORT: HISTIDINE-BINDING PROTEIN AND hisP PRO- TEIN. By Giovanna Ferro-Luzzi Ames and Julia Lever 1096

,8-GALACTOSIDE TRANSPORT IN BACTERIAL MEMBRANE PREPARATIONS: ENERGY

COUPLING VIA MEMBRANE-BOUND D-LACTIC DEHYDROGENASE

.By Eugene M. Barnes, Jr., and H. R. Kaback 1190 ENERGY-COUPLING MECHANISMS IN MITOCHONDRIA: KINETIC, SPECTROSCOPIC, AND

THERMODYNAMIC PROPERTIES OF AN ENERGY TRANSDUCING FORM OF CYTOCHROME b .By B. Chance, D. F. Wilson, P. L. Dutton, and M. EreciEieka 1175

In Vitro TRANSCRIPTION OF A TRANSFER RNA GENE. . By Violet Daniel, Sara Sarid, Jacques S. Beckmann, and Uriel Z. Littauer 1260

PROINSULIN: CRYSTALLIZATION AND PRELIMINARY X-RAY DIFFRACTION STUDIES

.By W. Wardle Fullerton, Reginald Potter, and Barbara W. Low 1213 (ERRATUM, 64, 283) FRACTIONATION OF PHYTOHEMAGGLUTININ, I. PURIFICATION OF

THE RNA AND DNA SYNTHESIS-STIMULATING SUBSTANCES AND EVIDENCE THAT THEY ARE NOT PROTEINS . By M. L. Goldberg, W. Rosenau, and G. C. Burke 1306



viii CONTENTS

SPECIFIC BINDING OF LEUCYL TRANSFER RNA TO AN IMMATURE FORM OF L-THREONINE

DEAMINASE: ITS IMPLICATIONS IN REPRESSION

.By G. Wesley Hatfield and R. 0. Burns 1027 ANALYSIS OF T4 PHAGE PROTEINS, I. CONVERSION OF PRECURSOR PROTEINS INTO

LOWER MOLECULAR WEIGHT PEPTIDES DURING NORMAL CAPSID FORMATION .

By Junko Hosoda and Ric Cone 1275 SUBUNIT HETEROGENEITY IN HUMAN SERUM BETA LIPOPROTEIN.

.By J. P. Kane, E. G. Richards, and R. J. Havel 1075 APPLICATION OF 129I MOSSBAUER EFFECT TO BIOLOGICAL SYSTEMS: STUDIES WITH

HEME MODELS.

By M. Pasternak, Peter G. Debrunner, G. DePasquali, Lowell P. Hager, and L. Yeoman 1142 PROMITOCHONDRIA OF ANAEROBICALLY GROWN YEAST, IV. CONVERSION INTO RESPIR-

ING MITOCHONDRIA . By H. Plattner, M. M. Salpeter, J. Saltzgaber, and G. Schatz 1252 IRON: ITS INTRACELLULAR LOCALIZATION AND POSSIBLE ROLE IN CELL DIVISION .

.By Elliott Robbins and Thoru Pederson 1244 IMMUNOGLOBULIN SYNTHESIS AND SECRETION, V. INCORPORATION OF LEUCINE AND

GLUCOSAMINE INTO IMMUNOGLOBULIN ON FREE AND BOUND POLYRIBOSOMES .

.By Charles J. Sherr and Jonathan W. Uhr 1183 SEQUENTIAL TRANSLATION OF TRINUCLEOTIDE CODONS FOR PEPTIDE BOND FORMATION,

TRANSLOCATION, AND TERMINATION. By Richard K. Tompkins 1164 SPIN-STATE CHANGES IN CYTOCHROME P-450cam ON BINDING OF SPECIFIC SUBSTRATES .

.- . . . . . . . . . . . . . . . By R. Tsai, C. A. Yu, I. C. Gunsalus, J. Peisach, W. Blumberg, W. H. Orme-Johnson, and H. Beinert 1157

PROTEIN CHAIN INITIATION IN RABBIT RETICULOCYTES.

. . . . . . . . . . By David B. Wilson and Howard M. Dintzis 1282 OXIDIZED RNASE AS A PROTEIN MODEL HAVING No CONTRIBUTION TO THE HYDROGEN

EXCHANGE RATE FROM CONFORMATIONAL RESTRICTIONS.

.By C. K. Woodward and Andreas Rosenberg 1067

BOTANY

VIRUSLIKE PARTICLES IN NUCLEI OF CULTURED PLANT CELLS WHICH HAVE LOST THE ABILITY TO DIFFERENTIATE. By R. D. Sjolund and C. Y. Shih 25

(ERRATUM, 64, 287).-QUANTITATIVE STUDY OF CLINAL VARIATION IN Juniperus virgini- ana USING TERPENOID DATA.

.By R. H. Flake, Ernst von Rudloff, and B. L. Turner 218 MITOCHONDRIAL LONGEVITY In Vitro: THE RETENTION OF RESPIRATORY CONTROL

.By Roger Romani and Anna Monadjem 869 A HUMMINGBIRD-POLLINATED SPECIES OF BORAGINACEAE IN THE ARIZONA FLORA .

.By Verne Grant and Karen A. Grant 917 ALTERATION OF GENE CONVERSION PATTERNS IN Sordariafimicola BY SUPPLEMENTATION

WITH DNA BASES. By Y. Kitani and Lindsay S. Olive 1290 ACETYLENE REDUCTION ASSAY FOR DETERMINATION OF PHOSPHORUS AVAILABILITY IN

WISCONSIN LAKES . By W. D. P. Stewart, G. P. Fitzgerald, and R. H. Burris 1104

GENETICS

FURTHER STUDIES ON LOSS OF T-ANTIGEN FROM SOMATIC HYBRIDS BETWEEN MOUSE CELLS AND SV40-TRANSFORMED HUMAN CELLS. . . By Mary C. Weiss 79

SELECTION AND POLYGENIC CHARACTERS .By Montgomery Slatkin 87 PREMEIOTIC DNA SYNTHESIS AND THE TIME OF CHROMOSOME PAIRING IN Locusta migra-

toria .By Peter B. AMoens 94 STUDIES OF LP-LIPOPROTEIN AS A QUANTITATIVE GENETIC TRAIT.

.By Nancy R. Harvie and Jane S. Schultz 99



CONTENTS ix

THE PHENOMENON OF "VESTING" IN RING CHROMOSOMES AND ITS ROLE IN THE MUTA-

TION THEORY AND UNDERSTANDING OF THE MECHANISM OF CROSSING-OVER .

.By N. P. Dubinin and L. S. Nemtseva 211 SYNCHRONOUS REINITIATION OF CHROMOSOME B EPLICATION IN E. coli B/r AFTER NALI-

DIXIC ACID TREATMENT . . By C. B. Ward, M. W. Hane, and D. A. Glaser 365 TEMPORAL PHENOTYPIC HETEROGENEITY AS A SUBSTRATE1 FOR SELECTION .

. . . . . . By Harold W. Kerster and Donald A. Levin 370 ISOLATION OF TEMPERATURE-SENSITIVE MUTANTS OF L-CELLS . . . . By L. H.

Thompson, R. Mankovitz, R. M. Baker, J. E. Till, L. Siminovitch, and G. F. Whitmore 377 GENETIC EVOLUTION OF BACTERIOPHAGE, II. PHYSICAL LENGTH OF THE HOmOoIGOUS

REGION IN A HYBRID BETWEEN SEROLOGICALLY AND MORPHOLOGICALLY UNRELATED

PHAGES. By Hiroto Akiyoshi and Nobuto Yamamoto 385 (ERRATUM, 65, 200).-TOPOLOGICAL RELATIONSHIP OF PROPHAGE X TO THE BACTERIAL

CHROMOSOME IN LYSOGENIC CELLS . By David Freifelder and Matthew Meselson 583 BACTERIOPHAGE LAMBDA: ABORTIVE INFECTION OF BACTERIA LYSOGENIC FOR PHAGE

P2 . By Gunnar Lindahl, Gianpiero Sironi, Harvey Bialy, and Richard Calendar 587 AGE-SPECIFIC SELECTION . . . . By Wyatt W. Anderson and Charles E. King 780 SEGREGATION OF FUNCTIONAL SEX FACTOR INTO MINICELLS

.By Leon R. Kass and Michael B. Yarmolinsky 815 SUPPRESSION OF CHEMICAL MUTAGENESIS IN BACTERIOPHAGFE T4 BY GENETICALLY

MODIFIED DNA POLYMERASES . . By John W. Drake and Elaine 0. Greening 823 REGULATION OF REPRESSOR EXPRESSION IN X.

. By H. Eisen, P. Brachet, L. Pereira da Silva, and F. Jacob 855 ISOZYME PATTERNS AND SEXUAL MORPHOGENESIS IN Schizophyllum.

. . . . . . I . . . . . By Chiut-Sheng Wang and John R. Raper 882 CHROMOSOME ABERRATIONS AMONG THE YANOMAMA INDIANS . . .- . By Arthur

D. Bloom, James V. Neel, Kyoo W. Choi, Shozo Iida, and Napoleon Chagnon 920 TRANSDUCTION BY BACTERIOPHAGE T. By Henry Drexler 1083 EVIDENCE FOR TRIPLICATE GENES FOR ALCOHOL DEHYDROGENASE IN HEXAPLOID WHEAT

.By Gary E. Hart 1136 (ERRATUM, 65, 226)-A STUDY ON THE TRANSCRIPTION OF THE POLYOMA VIRAL GENOME

.By J. Hudson, D. Goldstein, and R. Weil 1306 MAPPING OF A HUMAN GENETIC REGULATOR ELEMENT BY SOMATIC CELL GENETIC

ANALYSIS. . . By Robert J. Klebe, Tchaw-ren Chen, and Frank H. Ruddle 1220

IMMUNOLOGY

A VARIABLE REGION SUBCLASS OF HEAVY CHAINS COMMON TO IMMUNOGLOBULINS G, A, AND M AND CHARACTERIZED BY AN UNBLOCKED AMINo-TERMINAL RESIDUE .

. By A. C. Wang, J. R. L. Pink, H. H. Fudenberg, and J. Ohms 657 SPECIFIC SEPARATION OF CELLS ON AFFINITY COLUMNS.

.By Paolo Truffa-Bachi and Leon Wofsy 685 GENETIC CONTROL OF THE IMMUNE RESPONSE OF GUINEA PIGS TO LIMITING DOSES OF

BOVINE SERUM ALBUMIN: RELATIONSHIP TO THE POLY-L-LYSINE GENE . .By I. Green, J. K. Inman, and B. Benacerraf 1267

SPECIFIC INHIBITION OF TUMOR CELL DNA SYNTHESIS In Vitro BY LYMPHOCYTES FROM PERITONEAL EXUDATE OF IMMUNIZED SYNGENEIC GUINEA PIGS.

.By Joost J. Oppenheim, Berton Zbar, and Herbert Rapp 1119 ACTINOMYCIN D BEFORE AND DURING PRIMARY AND SECONDARY ANTI-FORSSMAN

IMMUNOGLOBULIN HEMOLYSIN RESPONSES IN RABBITS

.By William H. Taliaferro and Lucy Graves Taliaferro 1036 ACCELERATION BY THYMOSIN OF THE DEVELOPMENT OF RESISTANCE TO MURINE SAR-

COMA VIRUS-INDUCED TUMOR IN MICE . By Martin Zisblatt, Allen L. Goldstein, Frank Lilly, and Abraham White 1170



x CONTENTS

MEDICAL SCIENCES

COLONY INHIBITION OF FIBROBLASTS FROM CIITMERIC DoGS MEDIATED BY THE DOGS'

OWN LYMPHOCYTE8 AND SPECIFICAIIJY ABROGATED BY THEIR SERUM

. By I. Hellstr6m, K. E. Hellstrom, R. Storb, and E. D. Thomras 65 SUPPRESSION OF MALtGNANCY AND DIFFERENrTIATION IN MELANOTIC MELANOMA CELLS

.By Selma Silagi and Sarah A. Bruce 72 GLOBOID CELL LEUCODYSTROPHY (KRABBE'S DISEASE): DEFICIENCY OF GALACTO-

CEREBROSIDE ,3-GALACTOSIDASE . . . J3y Kunihiko Suzuki and Yoshiyuki Suzuki 302 TOLERANCE TO OPIOID NARCOTICS, II. CELLULAR TOLERANCE TO LEVORPHANOL IN

MOUSE BRAIN. By Judith A. Richter and Avram Goldstein 944 IMMUNOLOGICAL ALTERATION OF LEUKEMIC CELLS In ViVo AFTER TREATMENT WITH AN

ANTITUMOR DRUG.

. By E. Bonmassar, A. Bonmassar, S. Vadlamuli, and A. Goldin 1089 CHANGES IN CHROMOSOMES OF BONE MARROW AFTER INTRAVENOUS INJECTIONS OF

7,12-DIMETHYLBENZ(A)ANTHRACENE AND RELATED COMPOUNDS

. By E. Douglass Rees, S. K. Majumdar, and Amy Shuck 1228 CELLS INVOLVED IN CELL-MEDIATED AND TRANSPLANTATION IMMUNITY, II. A CON-

SIDERATION OF THE FUNCTIONAL IDENTITY OF THE CELLS INVOLVED IN BOTH

HUMORAL AND CELL MEDIATED IMMUNITY: A PHYLOGENETIC APPROACH

. . . . . . . . . . . . . . . . . . By Maxwell Richter 1127 IMMUNOASSAY OF PLASMA VASOPRESSIN IN MAN.

By Gary L. Robertson, Lester A. Klein, Jesse Roth, and Phillip Gorden 1298

MICROBIOLOGY

GLUCOSPHINGOLIPIDS OF PLASMA MEMBRANES OF CULTURED CELLS AND AN ENVELOPED

VIRUS (SV5) GROWN IN THESE CELLS

.By Hans-Dieter Klenk and Purnell W. Choppin 57 A CELL-ASSOCIATED FACTOR ESSENTIAL FOR FORMATION OF AN INFECTIOUS FORM OF

ROUS SARCOMA VIRUS . . . . By H. Hanafusa, T. Mliyamoto, and T. Hanafusa 314 LINK&GE BETWEEN THE POLY-L-LYSINE GENE AND THE LOCUS CONTROLLING THE

MAJOR HISTOCOMPATIBILITY ANTIGENS IN STRAIN 2 GUINEA PIGS.

By Leonard Ellman, Ira Green, William J. Martin, and Baruj Benacerraf 322 DNA COMPLEMENTARY TO VIRAL RNA IN LEUKEMIC CELLS INDUCED BY AVIAN MYELO-

BLASTOSIS VIRUS. By M. A. Baluda and D. P. Nayak 329 EVIDENCE FOR CONTROL OF SYNTHESIS OF THE VARIABLE REGIONS OF THE HEAVY

CHAINS OF IMMUNOGLOBULINS G AND M BY THE SAME GENE. By A. C. Wang, S. K. Wilson, J. E. Hopper, H. H. Fudenberg, and A. Nisonoff 337

LE3uKERMIA VIRUSES ASSOCIATED WITH MOUSE MYELOMA CELLS. . By J. Wattson, P. Ralph, S. Sarkar, and Melvin Cohn 344

GE.NE.TIC CONTROL OF THE CELL-DIVISION CYCLE IN YEAST, I. DETECTION OF MUTANTS . By Leland H. Hartwell, Joseph Culotti, and Brian Reid 352

(ERRATUM, 65, 81).-TRANS-SPECIES OF DEFECTIVE GENOMES OF MURINE SARCOMA

VIRUS FROM HAMSTER TUMOR CELLS WITH HELPER FELINE LEUKEMIA VIRUS . By Padman S. Sarma, T. Log, and Robert J. Huebner 583

INACTIVATING AND MUTAGENIC EFFECTS OF NITROSOGUANIDINE ON SIMIAN VIRUS 40 . . By Peter Tegtmeyer, Curt Dohan, Jr., and Catherine Reznikoff 745

CATABOLITE-INSENSITIVE REVERTANTS OF Lac PROMOTER MUTANTS . By Allen E. Silverstone, R. Rita Arditti, and Boris Magasanik 773

SYNTHESIS OF PROTEINS IN CELLS INFEICTED WITH HERPESVIRUS, VI. CHARACTERIZA-

TION OF THE PROTEINS OF THE VIRAL MEMBRANE.

.By Albert S. Kaplan and Tamar Ben-Porat 799



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