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Waters Positive Pressure-96 Processor...The Waters Positive Pressure-96 Processor offers...

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Waters Positive Pressure-96 Processor Waters Positive Pressure-96 Processor Highly Uniform Flow from Well-to-Well Superior Flow for Viscous Samples Highly Reproducible Assays Easy-to-Use Design The Waters Positive Pressure-96 Processor offers state-of-the-art operation for in 96-well and 1 cc flangeless cartridge formats. Each of the 96 holes in the processor is restricted in order to maintain constant pressure, even if all the plate well positions are not filled. Positive pressure processing offers many advantages over traditional methods. Reproducible Analyte Recoveries A major problem with conventional vacuum manifolds occurs as faster flowing channels in the 96-well plate run dry, allowing a free flow path for vacuum through the dry ports, thereby slowing the flow on the remaining wells. This may result in additional variation in well processing times, and possibly irreproducible analyte recoveries. The Waters Positive Pressure-96 Processor gasket with 96 independent ports provides restricted output of inert gas at every port. The processor exerts the same motive force to every port whether it had a full or dry well downstream or even no well access at all. This will provide highly uniform extractions which may improve the reproducibility of analyte recoveries. Improved Flow for Viscous Samples When processing viscous samples, vacuum manifolds often cannot provide adequate processing power, since the maximum pressure is limited to less than atmospheric pressure (approx. 15 psi). The Waters Positive Pressure-96 Processor allows for gas pressures up to 80 psi, supplying greater motive force for viscous sample flow. Adjusting the pressure up or down provides smooth and immediate flow response and high flow control on variable viscosity plasma samples on a 96-well plate. Fits all 96-Well Plate Formats Unlike many 96-well vacuum manifolds, the Waters Positive Pressure-96 Processor is self-adjusting to plate height, thus accommodating all 96-well plate formats, both modular and integrated, without the need for gaskets. Fits 96 1 cc Flangeless SPE Cartridges The Waters Positive Pressure-96 Processor provides the means to batch process Waters 1 cc 96 flangeless SPE cartridges. Waters 96-flangeless holder is designed to hold up to 96 1cc flangeless SPE cartridges, and to fit into the 96-position footprint of the manifold. The cartridge and holder assembly is then stacked onto the appropriate collection plate.
Transcript
Page 1: Waters Positive Pressure-96 Processor...The Waters Positive Pressure-96 Processor offers state-of-the-art operation for in 96-well and 1 cc flangeless cartridge formats. Each of Each

Waters Positive Pressure-96 Processor

Waters Positive Pressure-96 Processor■■ Highly Uniform Flow from Well-to-Well

■■ Superior Flow for Viscous Samples

■■ Highly Reproducible Assays

■■ Easy-to-Use Design

The Waters Positive Pressure-96 Processor offers state-of-the-art operation for in 96-well and 1 cc flangeless cartridge formats. Each of

the 96 holes in the processor is restricted in order to maintain constant pressure, even if all the plate well positions are not filled. Positive

pressure processing offers many advantages over traditional methods.

Reproducible Analyte Recoveries

A major problem with conventional vacuum manifolds occurs as faster flowing

channels in the 96-well plate run dry, allowing a free flow path for vacuum

through the dry ports, thereby slowing the flow on the remaining wells. This may

result in additional variation in well processing times, and possibly irreproducible

analyte recoveries. The Waters Positive Pressure-96 Processor gasket with 96

independent ports provides restricted output of inert gas at every port. The

processor exerts the same motive force to every port whether it had a full or dry

well downstream or even no well access at all. This will provide highly uniform

extractions which may improve the reproducibility of analyte recoveries.

Improved Flow for Viscous Samples

When processing viscous samples, vacuum manifolds often cannot provide

adequate processing power, since the maximum pressure is limited to less

than atmospheric pressure (approx. 15 psi). The Waters Positive Pressure-96

Processor allows for gas pressures up to 80 psi, supplying greater motive force

for viscous sample flow. Adjusting the pressure up or down provides smooth

and immediate flow response and high flow control on variable viscosity

plasma samples on a 96-well plate.

Fits all 96-Well Plate Formats

Unlike many 96-well vacuum manifolds, the Waters Positive Pressure-96 Processor

is self-adjusting to plate height, thus accommodating all 96-well plate

formats, both modular and integrated, without the need for gaskets.

Fits 96 1 cc Flangeless SPE Cartridges

The Waters Positive Pressure-96 Processor provides the means to batch process

Waters 1 cc 96 flangeless SPE cartridges. Waters 96-flangeless holder is

designed to hold up to 96 1cc flangeless SPE cartridges, and to fit into the

96-position footprint of the manifold. The cartridge and holder assembly is

then stacked onto the appropriate collection plate.

Page 2: Waters Positive Pressure-96 Processor...The Waters Positive Pressure-96 Processor offers state-of-the-art operation for in 96-well and 1 cc flangeless cartridge formats. Each of Each

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com

Waters and T he Science of What’s Possible are trademarks of Waters Corporation.

©2013 Waters Corporation. Produced in the U.S.A.April 2013 720003453EN KP-PDF

Easy-to-Use Design

The Waters Positive Pressure-96 Processor is easy to use. Simply

assemble the loaded SPE and collection plates, and position the

assembly on the processor slide tray platform. Slide the tray

to the rear of the processor until the assembly stops under the

manifold. Two toggle switches on the sides of the unit activate the

compression mechanism. If the user lets go of the switches too

soon the manifold mechanism will automatically open. This unique

built-in safety feature is designed to keep the user hands clear of

moving parts during the compression and decompression cycle,

ensuring the safety of the operator.

ORDERING INFORMATION

Description Qty/Pkg Part Number

Waters Positive Pressure-96 Processor

1 186006961

µElution Positive Pressure Spacer

1 405006528

96 1 cc Flangeless Cartridges Holder

1 186005523

96-well 1 mL Collection Plate

50 186002481

96-well 2 mL Collection Plate

50 186002482

24-well 10 mL Waste Collection Plate

1 186005586

Polypropylene Cap Mat for 1 mL Plate

50 186002483

Polypropylene Cap Mat for 2 mL Plate

50 186002484

Gas Supply Adapter, includes 1/8"-1/4" NPT Fitting, 6ft. of 1/4” Tubing

1 186005524

96 Place Sealing Gasket 1 186005522

Handling Viscous Samples

The Waters Positive Pressure-96 gas delivery system can provide

rapid gas flow to the manifold. This flow is controlled by adjusting

the regulator located below the pressure gauge labeled “MAX

FLOW.” The pressure range is 0-80 psi and is used to maximize

flow through the 96-well plate for processing viscous samples,

and reduces the the risk of well plugging by such samples.

Superior SPE Flow Control

The Waters Positive Pressure-96 Processor exerts fine control

on the flow though the plate. The inert gas flow can be adjusted

by switching the SPE flow rate selector to the left (LOW FLOW

setting). The gas is delivered to the Waters Positive Pressure-96

Processor through the adjustable flow regulator and through the

rotameter (on the left) controlled by the needle valve located at

its base. This will provide a uniform and controlled flow through

the entire 96-well plate without impact on flow from empty wells.


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