Waters Positive Pressure-96 Processor
Waters Positive Pressure-96 Processor■■ Highly Uniform Flow from Well-to-Well
■■ Superior Flow for Viscous Samples
■■ Highly Reproducible Assays
■■ Easy-to-Use Design
The Waters Positive Pressure-96 Processor offers state-of-the-art operation for in 96-well and 1 cc flangeless cartridge formats. Each of
the 96 holes in the processor is restricted in order to maintain constant pressure, even if all the plate well positions are not filled. Positive
pressure processing offers many advantages over traditional methods.
Reproducible Analyte Recoveries
A major problem with conventional vacuum manifolds occurs as faster flowing
channels in the 96-well plate run dry, allowing a free flow path for vacuum
through the dry ports, thereby slowing the flow on the remaining wells. This may
result in additional variation in well processing times, and possibly irreproducible
analyte recoveries. The Waters Positive Pressure-96 Processor gasket with 96
independent ports provides restricted output of inert gas at every port. The
processor exerts the same motive force to every port whether it had a full or dry
well downstream or even no well access at all. This will provide highly uniform
extractions which may improve the reproducibility of analyte recoveries.
Improved Flow for Viscous Samples
When processing viscous samples, vacuum manifolds often cannot provide
adequate processing power, since the maximum pressure is limited to less
than atmospheric pressure (approx. 15 psi). The Waters Positive Pressure-96
Processor allows for gas pressures up to 80 psi, supplying greater motive force
for viscous sample flow. Adjusting the pressure up or down provides smooth
and immediate flow response and high flow control on variable viscosity
plasma samples on a 96-well plate.
Fits all 96-Well Plate Formats
Unlike many 96-well vacuum manifolds, the Waters Positive Pressure-96 Processor
is self-adjusting to plate height, thus accommodating all 96-well plate
formats, both modular and integrated, without the need for gaskets.
Fits 96 1 cc Flangeless SPE Cartridges
The Waters Positive Pressure-96 Processor provides the means to batch process
Waters 1 cc 96 flangeless SPE cartridges. Waters 96-flangeless holder is
designed to hold up to 96 1cc flangeless SPE cartridges, and to fit into the
96-position footprint of the manifold. The cartridge and holder assembly is
then stacked onto the appropriate collection plate.
Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com
Waters and T he Science of What’s Possible are trademarks of Waters Corporation.
©2013 Waters Corporation. Produced in the U.S.A.April 2013 720003453EN KP-PDF
Easy-to-Use Design
The Waters Positive Pressure-96 Processor is easy to use. Simply
assemble the loaded SPE and collection plates, and position the
assembly on the processor slide tray platform. Slide the tray
to the rear of the processor until the assembly stops under the
manifold. Two toggle switches on the sides of the unit activate the
compression mechanism. If the user lets go of the switches too
soon the manifold mechanism will automatically open. This unique
built-in safety feature is designed to keep the user hands clear of
moving parts during the compression and decompression cycle,
ensuring the safety of the operator.
ORDERING INFORMATION
Description Qty/Pkg Part Number
Waters Positive Pressure-96 Processor
1 186006961
µElution Positive Pressure Spacer
1 405006528
96 1 cc Flangeless Cartridges Holder
1 186005523
96-well 1 mL Collection Plate
50 186002481
96-well 2 mL Collection Plate
50 186002482
24-well 10 mL Waste Collection Plate
1 186005586
Polypropylene Cap Mat for 1 mL Plate
50 186002483
Polypropylene Cap Mat for 2 mL Plate
50 186002484
Gas Supply Adapter, includes 1/8"-1/4" NPT Fitting, 6ft. of 1/4” Tubing
1 186005524
96 Place Sealing Gasket 1 186005522
Handling Viscous Samples
The Waters Positive Pressure-96 gas delivery system can provide
rapid gas flow to the manifold. This flow is controlled by adjusting
the regulator located below the pressure gauge labeled “MAX
FLOW.” The pressure range is 0-80 psi and is used to maximize
flow through the 96-well plate for processing viscous samples,
and reduces the the risk of well plugging by such samples.
Superior SPE Flow Control
The Waters Positive Pressure-96 Processor exerts fine control
on the flow though the plate. The inert gas flow can be adjusted
by switching the SPE flow rate selector to the left (LOW FLOW
setting). The gas is delivered to the Waters Positive Pressure-96
Processor through the adjustable flow regulator and through the
rotameter (on the left) controlled by the needle valve located at
its base. This will provide a uniform and controlled flow through
the entire 96-well plate without impact on flow from empty wells.