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*Over 200 delicious recipes for beef, pork,

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*10-page table with times and temperatures

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Sous Vide Cooking and Chemistry

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ACS WEBINARS™ May 9, 2013

8

Dr. Sara Risch

Popz Europe

Dr. Douglas Baldwin

Author

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Sous vide cooking and chemistry

Douglas E. Baldwin

Department of Applied MathematicsUniversity of Colorado at Boulder

ACS Webinar — May 9, 2013

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How often do you cook?

(a) Cook almost everyday.

(b) Not daily, but quite a lot.

(c) Only for special occasions.

(d) Almost never.

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sous vide /su: ’vi:d/ adjectival & adverbial phr.L20. [ French, from sous under+ vide vacuum.]Of food: (prepared) by cooking invacuumized pouches at preciselycontrolled temperatures.

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Optimal food temperatures▶ Beef, lamb, and pork

∼50 °C Rare∼55 °C Medium-rare∼60 °C Medium>70 °C Well done

▶ Fish and shellfish∼49 °C Medium-rare

▶ Baked goods∼90 °C Breads, rolls, muffins, etc.

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Common kitchen heat sources

∼70 °C Slow-cooker

100 °C Boiling water

125–200 °C Oven

150–250 °C Skillet

200–350 °C Grill

1500–2000 °C Blowtorch

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Sous vide cooking:▶ Cook at the food’s optimal temperature.▶ If 55 °C is medium-rare,

then use a 55 °C water-bath⇒meat can’t exceed 55 °C⇒ nothing overcooked⇒medium-rare from edge to edge

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Vacuum-sealing benefits

▶ Removing air improves heat transfer▶ Increases shelf-life

• Food can’t be recontaminated• Inhibits off-flavors from oxidation• Reduces aerobic bacterial growth

▶ Improves nutrition and flavor• Stops flavor volatile evaporation• Stops nutrients leaching into water

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Outline

▶ Soft-cooked eggs• Protein denaturation

▶ Beef chuck roast• How heating changes meat• Extended heating tenderizes

▶ Chicken breasts• Pasteurizing for safety

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Protein denaturation

Heat

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Protein denaturation

▶ Heat — cooking, baking, …▶ Mechanical agitation — whipping▶ pH change — vinegar, lemon juice, …▶ Inorganic salts — curing and brining▶ Organic compounds — alcohol marinades▶ Detergents — cleanup

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Yolk temperatures in 75 °C water

5 10 15 20 25 30Time HminL

10

20

30

40

50

60

70

Temp H°CL

Measured yolk temperatures of 14 “large” eggs.

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Reaction rate

▶ Temperature• Arrhenius reactions:

10 °C increase roughly doubles rate• Yolk denaturation:

1 °C increase roughly doubles rate▶ Catalysts

• Enzymes catalyze biochemical reactions▶ Concentration

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When you grill meat or poultry, do you

(a) always use a thermometer to see when it’sdone,

(b) sometimes use a thermometer, or

(c) never use a thermometer?

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Meat proteinsMeat’s about 75% water, 20% protein,

and 5% fat and other substances.Proteins:

▶ Muscle fibers— mostly myosin & actin

▶ Soluble proteins— mostly enzymes and myoglobin

▶ Connective tissue— mostly collagen, less elastin

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Meat protein denaturing

In traditional cooking when heating:▶ Muscle fibers shrink

starting 35–40 °C up to ∼80 °C▶ Soluble proteins aggregate and gel

starting ∼40 °C and finishing ∼60 °C▶ Connective tissues shrink

starting ∼60 andmore intensely above ∼65 °C

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Doneness

50 °C Rare — muscle fibers andsoluble proteins start denaturing

55 °C Medium-rare — more muscle fibers andsoluble proteins denature

60 °C Medium— most soluble proteins denatured

>70 °C Well done— connective tissue start denaturing— muscle fibers squeeze out water

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If you eat beef, do you prefer it

(a) rare,

(b) medium-rare,

(c) medium, or

(d) well done?

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If 55 °C is medium-rare,why not cook at 55°C?

You can with sous vide cooking.

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Slower reactions tenderize

Holding meat at 55–60 °C for hoursto days increases tenderness because

▶ Enzymes can catalyzeconnective-tissue protein hydrolysis

▶ Collagen slowly starts to denaturearound 51 to 53 °C

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Beef chuck roast

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Meat flavor

1. Browning or Maillard reaction• Roast and savory flavors• Starts noticably ∼130 °C• Good browning starts ∼150 °C

2. Fat makes• lamb taste like lamb and• beef taste like beef.

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Maillard reaction

▶ Complex reaction betweenamino acids and reducing sugars

▶ Produces hundreds of reaction by-products▶ Reaction rate increased by

• Increasing temperature• Adding a reducing sugar• Increasing the pH

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Rapid browning methods

▶ Beef and lamb• Butane blowtorch• Very hot grill or broiler

▶ Chicken and pork• Pan with smoking-hot oil• Shimmering oil with 4% glucose wash• Very hot grill or broiler

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Beef chuck roast

1. Preheat water to 55 °C for medium-rare

2. () Pre-sear with blowtorch or grill

3. Vacuum-seal roast in a large pouch

4. Put into water bath for 1–2 days

5. Remove from pouch and pat dry

6. Sear each side to a mahogany brown

7. Season and serve immediately

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When do you stop cooking a chicken breasts?

(a) When it’s juices run clear.

(b) When it’s white when you cut into it.

(c) When it reaches 75 °C/165 °F.

(d) When it’s dry and stringy.

(e) Some other criteria.

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Food microorganisms

..Spoilage.

Beneficial

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Pathogenic

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Many ways to reduce pathogens

▶ Heat — both time and temp important▶ Inorganic salts — curing▶ pH changes — acidifying▶ Herbs and spices — essential oils▶ Mechanical agitation — very high pressures▶ Alcohol — marinades▶ Ionizing radiation — not for home kitchens

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“Danger Zone”?

▶ Traditional “danger zone” is 5 to 60 °C▶ Food pathogens don’t grow below−1.3 °C▶ Food pathogens don’t grow above 52.3 °C▶ Dangerous growth takes days at 5 °C▶ Pasteurization takes 43 min at 60 °C

but 3 hr 20 min at 55 °C

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Pasteurization reduces risk

▶ Reduce but can’t eliminate pathogens▶ Healthy may need 105 to 109 to get sick▶ Immunocompromised 1–10/g to get sick▶ 15–20% of US immunocompromised▶ 106 → 1 reduction usually recommended

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Pathogens of interest

▶ Salmonella species▶ Pathogenic strains of Escherichia coli▶ Listeria monocytogenes— the toughest

▶ 106 → 1 after 2 min at 70 °C▶ 106 → 1 after 20 min at 62.5 °C▶ 106 → 1 after 200 min at 55 °C

▶ Spore forms, like the Clostridium species

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Sous vide chicken breasts

1. Preheat water bath to 60 °C.

2. Individually vacuum-seal the breasts.

3. Put sealed pouches in water bath.

4. Cook them for at least 2 hours.

5. Remove from bath and pouches. Pat dry.

6. Sear in a skillet with smoking-hot oil.

7. Serve immediately.

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Balance time and temperature

..Temperature

.Time

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Safety

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Doneness

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Texture

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Pork chops

▶ Medium-rare to medium⇒ 55–60 °C▶ Moderately tender⇒ short to moderate cooking times▶ 60 °C & 20 mm thick⇒ 1½ hours to pasteurize

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Filet mignon

▶ Rare to medium-rare⇒ 50–55 °C▶ Prized tenderness⇒ short cooking time▶ 50–55 °C & short time⇒ can’t pasteurize⇒ healthy people only▶ 50 °C⇒ pathogen growth⇒minimize time▶ 55 °C⇒ short time or mushy texture

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Additional Resources

▶ www.DouglasBaldwin.com▶ Free sous vide cooking guide▶ YouTube video demos▶ Review article [IJGFS vol. 1 (2012) pp. 15–30]

▶ D.B.’s Sous Vide for the Home Cook (2010)▶ Over 200 recipes▶ Less technical than website or review article

▶ Other food science books:▶ H. McGee’s On Food and Cooking (2004)▶ N. Myhrvold et al.’sModernist Cuisine (2011)

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Sous Vide Cooking and Chemistry

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ACS WEBINARS™ May 9, 2013

2

Dr. Sara Risch

Popz Europe

Dr. Douglas Baldwin

Author

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