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Contrasting Catalytic Profiles of Multi-Heme Nitrite Reductases Containing CxxCK Heme
Binding Motifs.
Rose-Marie A. S. Doyle1, Sophie J. Marritt1, James D. Gwyer1, Thomas G. Lowe1,
Tamara V. Tikhonova2, Vladimir O. Popov2,3, Myles R. Cheesman1 and Julea N. Butt1
1Centre for Molecular and Structural Biochemistry, School of Chemistry and School of
Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, U.K.2A. N. Bakh Institute of Biochemistry RAS, Leninsky pr.33, 119071 Moscow, Russia3NBICS Centre of Russian National Research Centre "Kurchatov Institute", Akademika
Kurchatova sq. 1, Moscow 123182, Russia
*Running title: Nitrite Ammonification by Multi-Heme Cytochromes.
To whom correspondence should be addressed: Julea Butt, School of Chemistry, University
of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK, Tel: 00 44 1603 593877, Fax:
00 44 1603 592003, E-mail: [email protected]
2The abbreviations used are: EcNrfA, pentaheme cytochrome with nitrite reductase activity from
Escherichia coli; Em, mid-point potential; LMCT, ligand-to-metal charge-transfer; MCD,
magnetic circular dichroism; MOTTLE, MCD compatible Optically Transparent Thin Layer
Electrochemistry; nIR, near infra-red; PGE, pyrolytic graphite edge; SHE, standard hydrogen
electrode; TvNiR, octaheme nitrite reductase from Thioalkalivibrio nitratireducens, WsNrfA,
pentaheme cytochrome with nitrite reductase activity from Wolinella succinogenes.
Keywords: Magnetic Circular Dichroism, Nitrogen Cycle, Cytochrome, Modified Tyrosine,
Reductive Activation
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SUMMARY
The multi-heme cytochromes from Thioalkalivibrio nitratireducens, TvNiR, and Escherichia
coli, EcNrfA, reduce nitrite to ammonium. Both enzymes contain His/His ligated hemes to
deliver electrons to their active sites where a Lys-ligated heme has a distal pocket containing
a catalytic triad of His, Tyr and Arg residues. Protein film electrochemistry reveals
significant differences in the catalytic properties of these enzymes. TvNiR, but not EcNrfA,
requires reductive activation. Spectroelectrochemistry implicates reduction of His/His ligated
heme(s) as being key to this process which restricts the rate of hydroxide binding to the ferric
form of the active site heme. The KM describing nitrite reduction by EcNrfA varies with pH in
a sigmoidal manner that is consistent with its modulation by (de-)protonation of a residue
with pKa ≈ 7.6. This residue is proposed to be the catalytic His in the distal pocket. By
contrast the KM for nitrite reduction by TvNiR decreases approximately linearly with increase
of pH such that different features of the mechanism define this parameter for TvNiR. In other
regards the catalytic properties of TvNiR and EcNrfA are similar, namely, the pH dependence
of Vmax and the nitrite dependence of the catalytic current-potential profiles resolved by cyclic
voltammetry, such that the determinants of these properties the enzymes appear to be
conserved.
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Numerous microbes possess a single enzyme that can reduce nitrite to ammonium without
the release of detectable intermediates (1,2). The coordinated delivery of electrons and
protons to the substrate ensures that two N-O bonds are cleaved to achieve the overall
transformation NO2- + 6e- + 8H+ → NH4
+ + 2H2O without releasing detectable intermediates.
The result is a reaction that removes a potent cytotoxin, serves as a step to assimilate
inorganic nitrogen and that may contribute to the generation of a trans-membrane proton
gradient. The chemistry that occurs during nitrite ammonification is all the more remarkable
when it is recognized that an alternative route to the same transformation employs four
enzymes with nitric oxide, nitrous oxide and di-nitrogen as discrete intermediates.
One class of nitrite ammonifying enzyme is formed by the multi-heme cytochromes c that
contain a CxxCK heme-binding motif (3-10). This motif provides the proximal lysine ligand
to the active site heme in addition to cysteine residues that bind the heme through thioether
bonds. The sidechains of three conserved residues, namely, His, Tyr and Arg, define the
distal pocket of the active site, Fig. 1. Positioned ca. 11 Å from the active site heme iron there
is a Ca2+ ion. This contributes to a positive electrostatic potential attracting nitrite to the active
site and may play a role in supplying protons to the substrate and catalytic intermediates
(11,12).
Of the CxxCK containing multi-heme cytochromes c those with five hemes, termed NrfA,
are the most extensively characterized. These enzymes contain four low-spin c-hemes, bound
through CxxCH heme-binding motifs, which deliver electrons to the active site heme that is
high-spin in the absence of substrate or inhibitors (3-8). The low-spin hemes have His/His
axial ligation and are positioned as two branches converging on the active site from distinct
regions of the protein surface. One branch supports electron exchange between the active site
and the cellular redox partner. The second branch allows for electron exchange between NrfA
subunits (13) in the homodimeric structures resolved by X-ray crystallography (3-8).
With a view to understanding the catalytic mechanism of NrfA several spectroscopic and
electrochemical methods have been used with the aim of resolving the redox chemistry of the
hemes. There is agreement that the heme reduction potentials span several hundred mV
(5,8,14-17). However, there are some differences in the descriptions, Table 1, that are not
surprising given the large number of hemes that makes it extremely difficult to
unambiguously attribute the contributions from individual centers. X-band electron
paramagnetic resonance spectra of the oxidized enzymes are complex and difficult to
interpret quantitatively (5,17). The low-spin ferric hemes with perpendicular axial ligand
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planes, if not spin-coupled, will give rise to resonances of intrinsically low intensity and in
addition there is strong spin-coupling between some pairs of adjacent hemes. In contrast,
electronic absorbance, magnetic circular dichroism (MCD) and resonance Raman
spectroscopies are able to unambiguously identify features from all the low-spin His/His
ligated hemes and from the high-spin Lysine ligated heme of the active site (15,17).
Employing these methods in spectroelectrochemistry, where the sample is equilibrated with
the potential applied to an electrode, has resolved redox properties of the NrfA hemes
(8,15,17) while avoiding the use of the common reductant sodium dithionite that can generate
anions that bind to the active site (18).
Cyclic voltammetry can resolve reduction potentials of the NrfA hemes but by itself does
not provide structural information to allow their correlation to individual centers (8,15,16).
This limitation can be overcome when NrfA is adsorbed as a film on an electrode that allows
for spectroscopic characterization of the enzyme in the oxidation state that is defined by the
applied electrode potential (15,17). A further and very significant strength of protein-film
electrochemistry (PFE) is that it allows the catalytic properties of an enzyme to be resolved as
a function of electrochemical potential, time and concentration. As a consequence the
information afforded by PFE complements that from other techniques. In addition to defining
the kinetic parameters, KM and Vmax, describing steady-state catalysis it is possible to quantify
catalytic bias and the rates and mechanisms of inhibition and (in)activation whether
reversible or irreversible (19).
Largely for the reasons above, PFE has been applied to resolve the catalytic properties of
NrfAs from a number of organisms, see for example (13,16,20,21). However, we are not
aware that the impact of pH on nitrite ammonification by CxxCK containing multi-heme
cytochromes c has received significant attention. Given the importance of protons to nitrite
ammonification we address this here in a PFE study that reveals differences in the kinetic
parameters that describe the pH dependence of nitrite reduction by Escherichia coli NrfA
(EcNrfA) and a closely related enzyme that contains a CxxCK ligated c-heme, namely,
Thioalkalivibrio nitratireducens octaheme nitrite reductase (TvNiR) (9). The C-terminal
domain of TvNiR contains the constellation of five c-type hemes and axial ligand sets
conserved in NrfA. The three additional c-hemes are bound to the N-terminal domain, have
His/His axial ligation and extend one branch of hemes leading to the active site. TvNiR
catalyzes nitrite ammonification at a lysine ligated c-type heme that, like NrfA, has a distal
pocket defined by His, Tyr and Arg side-chains. In a difference to NrfA the Tyr is modified
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at the ortho-position by a covalent bond to the sulfur of the neighboring cysteine residue, Fig.
1B. To reveal the heme reduction potentials and axial ligand sets that underpin catalysis by
TvNiR their magneto-optical and spectroelectrochemical characterization is presented here.
These reveal further differences in the properties of the two enzymes.
Experimental Procedures
Protein Samples and Reagents - The octaheme nitrite reductase from Thioalkalivibrio
nitratireducens (TvNiR) was purified according to the published method (22). Purified
protein in 0.1 M potassium phosphate, pH 7.0 was stored as aliquots in liquid nitrogen.
Protein concentration was quantified by electronic absorption spectroscopy using an
extinction coefficient of 410 nm = 884 000 M-1 cm-1 for air equilibrated (i.e. oxidized) TvNiR.
This extinction coefficient was determined following quantification of the c-heme content by
the pyridine hemochrome method that was calibrated with horse heart cytochrome c (408 nm =
106 000 M-1 cm-1) (23,24). EcNrfA was purified and characterized as described previously
(5). All other reagents were of at least Analar quality and solutions were prepared in water
with resistivity > 18 M cm (Purelab Maxima, ELGA).
Spectroscopic Analysis of Solutions of TvNiR – Samples for ambient temperature MCD of
oxidized TvNiR were prepared in 50 mM Hepes, 100 mM NaCl, pH 7.0. Measurements in the
near infra-red region and at low temperature were performed on samples prepared in
deuterium oxide, 50 mM Hepes, pH* 7.0 with 50% glycerol added as glassing agent and
where pH* is the apparent pH measured in D2O based solutions using standard glass
electrodes. Eu(II) reduced enzyme was prepared by addition of aliquots of 6 mM EuCl2, 50
mM Hepes, pH 7.0 to an anaerobic solution of TvNiR. Eu(II) was added until the electronic
absorption spectrum was typical of low-spin ferrous heme and unchanged by further
additions of EuCl2 at which point the sample was analyzed by MCD. The aqueous Eu(III/II)
couple has Em = -400 mV under the experimental conditions used here (25).
Spectroelectrochemical potentiometric titration of TvNiR monitored by MCD used the
method of Marritt et al (15) using MCD compatible optically transparent thin layer
electrochemistry (MOTTLE). The cell was of 0.5 mm path length and employed a gold mesh
working electrode with the following mediators each at 40 µM; ruthenium hexamine
chloride, trimethylhydroquinone, 5-hydroxynaphthoquinone, duroquinone, menadione, 9,10-
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anthraquinone 2,6 disulfonate, anthraquinone sulfonate, benzyl viologen, sulfonyl viologen
and methyl viologen. Spectra from MOTTLE are presented without correction for the zero-
field baseline because the time required to cycle the magnetic field of the super-conducting
magnet would preclude completion of potentiometric titrations in a practical time period.
MCD spectra were recorded on JASCO circular dichrographs, model J810 for the visible
region and model J730 for the near infrared region, in a magnetic field provided by an Oxford
Instruments superconducting magnet, model SM1 at 6 Tesla for ambient temperature and
model SM4 at 5 Tesla for low temperature measurements. It was not possible to access as
low potentials in the MOTTLE cell as when studying proteins adsorbed on SnO2 electrodes
(15,26). This is most likely due to proton reduction occurring at lower overpotential using the
gold-mesh electrodes. All potentials are reported versus the Standard Hydrogen Electrode
(SHE).
Protein Film Electrochemistry – To measure the catalytic activity of TvNiR, films were
prepared on freshly polished pyrolytic graphite edge (PGE) electrodes by exposure to 30 µM
TvNiR in ice-cold 50 mM Hepes, 100 mM NaCl, pH 7.0 for a few seconds using the
procedures previously described for studies of EcNrfA (20). A nitrite stock solution (500
mM) was freshly prepared for each day of experiments and diluted to the required
concentration. Cyclic voltammetry and chronoamperometry with rapid rotation of the PGE
working electrode were performed using a three-electrode cell configuration housed in a
Faraday cage and placed inside a N2-filled chamber as described previously (20). During
continuous cyclic voltammetry, and chronoamperometry, the catalytic current magnitudes
measured for both EcNrfA and TvNiR showed a first order exponential decay over time. The
catalytic current magnitudes were corrected for this effect prior to further analysis such as the
extraction of imax and KM values. Catalytic currents were recorded with electrode rotation at
3000 rpm at which speed they were free from limitation by the rate of nitrite delivery to the
enzyme film. Kinetic parameters were determined from the variation in catalytic current
magnitude (icat) at -550 vs SHE with nitrite concentration ([nitrite]) fitted to the Michaelis-
Menten equation:
reaction velocity = icat =imax [nitrite ]
K M + [ nitrite ]
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where KM is the Michaelis constant and imax is the maximum velocity (Vmax) presented as a
catalytic current. The pH dependence of imax (Vmax) was defined by film transfer experiments
whereby the catalytic current (ipH) of a given film was measured at the pH of interest, then at
pH 7 and then again at the pH of interest with the nitrite concentration in each solution equal
to the KM at that pH. Transfer of the film between identical solutions established that the
signal loss on film transfer was reproducible but greater than predicted by a first order
exponential decay over time. As a consequence, the value of imax relative to that at pH 7 was
given by the average of ipHx (initial)/ ipH7 and ipHx (final)/ ipH7. Absolute measurements of imax made
with a series of independently prepared films reproduced the variation of imax with pH found
in the film transfer experiments. The latter experiment produced a greater scatter of
individual data points due to the inherent variation, approximately 15%, in current magnitude
displayed by independently prepared films measured under identical conditions. This
variation is most likely to reflect variation in the population of electroactive molecules in the
film forming process. For NrfA the catalytic currents measured above pH 9 were too small
for reliable analysis.
Spectroelectrochemistry of TvNiR films adsorbed on mesoporous nanocrystalline SnO2
electrodes was achieved using minor modification of previously reported procedures (15).
Both optical windows of the electrochemical cell supported a SnO2 electrode of geometric
area 3 mm × 7 mm and 4 μm path length. TvNiR adsorbed onto the electrodes during 4 hours
of cyclic voltammetry (+200 to -400 mV) in an anaerobic solution of 15 M TvNiR, 50 mM
Hepes, 100 mM NaCl, pH 7.0. Prior to spectroelectrochemistry the electrodes were rinsed
thoroughly with 50 mM Hepes, 100 mM NaCl, pH 7.0 to remove non-adsorbed and loosely
bound protein.
Results
Magneto-Optical Characterization of TvNiR- Magnetic circular dichroism (MCD) was used
to assess the ligation- and spin-states of the hemes in solutions of TvNiR. The air-
equilibrated, as prepared enzyme displays a broad trough with a minimum at 620 nm, Fig.
2A. This feature appears in a spectral region where high-spin ferric hemes give rise to ligand-
to-metal charge-transfer (LMCT) bands at energies diagnostic of the chemical nature of the
axial ligands to the heme (27). For TvNiR the energy of this feature is indicative of a high-
spin ferric heme having ligation from a nitrogenous species and hydroxide. The MCD
intensity at 620 nm arising from such centers varies from 1 to 4 M-1 cm-1 T-1 per heme (27)
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and is approximately 2.6 M-1 cm-1 T-1 for TvNiR. This feature is then most reasonably
assigned to the active site heme that X-ray crystallography resolves with axial ligation by
lysine and an oxygen atom when substrates and inhibitors are absent (9).
In the Soret- and /-regions, 400 to 450 nm and 500 to 570 nm respectively, the MCD of
TvNiR shows features characteristic of low-spin ferric heme, Fig. 2A. There is no evidence
for ferrous-heme. Both low-spin and high-spin ferric heme give rise to a bisignate feature in
the Soret region. The peak-to-trough intensity of this feature is significantly greater for low-
spin than high-spin heme with typical values being, respectively, 150 M-1 cm-1 T-1 and 20 M-1
cm-1 T-1 per heme (28). For TvNiR the intensity of this feature is approximately 1100 M-1 cm-1
T-1 and so is consistent with a nearest integer ratio of seven low-spin ferric hemes per high-
spin heme. Low-spin ferric hemes display LMCT bands in the near infra-red region with
energies that reflect the chemical nature of their axial ligands (29). For TvNiR the single
positive band at 1500 nm with a vibrational sideband at shorter wavelengths (1200 to 1300
nm) is typical for His/His ligated ferric heme, Fig. 2B. The MCD provides no evidence for
low-spin hemes with other ligand sets.
To assess the nature of the hemes present in reduced TvNiR the enzyme was reduced with
aqueous Eu(II). Aliquots of Eu(II) were added until the electronic absorbance was unchanged
by further additions and typical of that from low-spin ferrous c-heme with maxima at 424,
524.5 and 554 nm, Fig. 2C inset. The ambient temperature MCD of the Eu(II) reduced TvNiR
in the visible and Soret regions showed features that could be confidently assigned to low-
spin, but not high-spin, ferrous heme (not shown). This could indicate an absence of high-
spin ferrous heme or simply the difficulty of resolving features characteristic of high-spin
ferrous heme in the presence of the much more intense features that arise from low-spin
ferrous heme. To resolve these possibilities MCD was performed at temperatures ≤ 100 K,
Fig. 2C.
The intensities of features from paramagnetic high-spin (S=2) states ≤ 100 K are strongly
temperature dependent whereas those from diamagnetic low-spin (S=0) states are temperature
independent (30). At the relatively high temperature of 100 K, the MCD of TvNiR that has
been reduced by aqueous Eu(II) is dominated by sharp bisignate features between 500 and
560 nm that are typical of low-spin ferrous heme, Fig. 2C. These features have the same
intensities at 100, 10 and 4.2 K since they arise from diamagnetic centers. By contrast the
intensities of the features in the Soret region increase significantly as the temperature is
lowered and must arise from paramagnets; the 440 nm peak and 427 nm trough are typical of
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high-spin ferrous heme. The only exception to this is a small positive and temperature
invariant feature at ≈ 422 nm that is the Soret band of the diamagnetic low-spin hemes. The
397 nm peak and 410/416 nm trough are characteristic of low-spin ferric heme (S = ½).
The absolute intensities of the MCD features at each temperature ≤ 100 K indicate that
addition of Eu(II) has achieved greater than 85% reduction of TvNiR (30). A single high-spin
ferrous heme, most likely to be that associated with the active site, is detected together with at
least six low-spin ferrous hemes. The MCD of air equilibrated and Eu(II) reduced TvNiR is
consistent with the hemes having the same axial ligation as resolved by X-ray diffraction
where the oxidation state of the enzyme is unknown.
Spectroelectrochemical Characterization of TvNiR –Initial analysis of TvNiR redox
chemistry was performed following adsorption of the enzyme onto optically transparent,
mesoporous, nanocrystalline SnO2 electrodes. With the electrode poised at potentials above
+50 mV the electronic absorbance spectra contained a Soret maximum at 410 nm alongside
weaker features between 500 and 600 nm, Fig. 3A. These features are typical of the oxidized
enzyme, e.g., Fig. 2C, and here they are superimposed on a slope due to scattering by the
SnO2 particles. Assuming the extinction coefficient of the adsorbed protein to be that of the
protein in solution, and given that the actual area of the electrode is 300 the geometric area
(31), the amount of adsorbed TvNiR was calculated as approximately 1 picomole cm-2 of the
actual area.
When the electrode was poised below -500 mV the electronic absorbance failed to resolve
features of the oxidized protein. Instead maxima at 423, 524 and 553 nm were displayed.
These are characteristic of reduced TvNiR and demonstrated that all the adsorbed protein was
able to equilibrate with the potential applied to the electrode, Fig. 3A. The non-turnover
peaks resolved by cyclic voltammetry of the adsorbed protein were not sufficiently defined to
be confidently resolved from the background of much larger and non-uniform currents arising
from charging of the nanostructured SnO2 (31,32). Nevertheless, unambiguous and
background free detection of the redox changes in adsorbed TvNiR was afforded by the
potential dependence of its electronic absorbance. This was independent of whether the
potentiometry was performed in an oxidative or reductive direction, Fig. 3A, inset.
The ferric/ferrous couple of low-spin, but not high-spin, c-heme contributes to the
change in absorbance at 553 nm. This is observed here between +160 and -450 mV and so
across a similarly low potential window to that where the NrfA low-spin hemes are redox
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active, Table 1 (8,14,15). This data is not readily resolved into the individual contributions of
the seven His/His ligated hemes in TvNiR. However, an indication of their macroscopic
reduction potentials, Em, can be presented if several assumptions are made, namely, that each
heme contributes equally to the total absorbance change and acts independently in a
reversible manner described by the Nernst equation for a one-electron (n = 1) process. With
these assumptions the least number of components required to describe the plot of 553 nm
absorbance versus potential is four with Em values of +100 (one heme), -80 (one heme), -200
(three hemes) and -370 (two hemes) mV, Fig. 3A inset. Within our model an uncertainty of
20 mV is associated with each Em to reflect the accuracy with which the absolute value of our
reference electrode is known.
In favorable circumstances the ferric/ferrous transformation of high-spin heme can be
detected through changes in the absorbance around 440 nm, e.g. (15,33). Such changes are
most readily identified in reduced minus oxidized difference spectra generated for closely
spaced equilibration potentials since the redox transformation of low-spin hemes can also
give rise to spectral changes at this wavelength. However, for TvNiR the difference spectra
are dominated by features typical of the ferric/ferrous transition of low-spin c-hemes with no
feature(s) that could be assigned to the corresponding transition of a high-spin heme, Fig. 3B.
This is most likely because the absorbance change per low-spin heme is intrinsically of much
larger magnitude than the absorbance change per high-spin heme, a situation that is
compounded in TvNiR by the presence of seven low-spin hemes for each high-spin center. As
a consequence evidence for redox transformation of the high-spin heme was sought from
MCD-monitored spectroelectrochemistry where the 620 nm trough measured at ambient
temperatures reports on the population of high-spin ferric heme with lysine/hydroxide
ligation.
The intrinsically low intensity of the 620 nm feature required spectroelectrochemistry of
highly concentrated TvNiR solutions. For this an optically transparent thin-layer
electrochemical cell employing a gold-mesh working electrode was used together with a
cocktail of redox mediators as described in the Experimental Procedures. The spectral
features arising from both the ferric and ferrous states of the low-spin hemes displayed fully
reversible behavior consistent with that displayed by the enzyme adsorbed on SnO2
electrodes (not shown) and equilibration with the applied potential was typically achieved
within 40 minutes. The active site heme behaved differently in that re-oxidation occurred
over a much longer time frame than reduction. The intensity of the 620 nm trough decreased
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to a negligible level as the potential of the sample was lowered from -200 mV to -275 mV,
Fig. 4A. However, even after 17 hrs exposure to +180 mV the intensity of this feature was
not restored to that displayed by the protein as purified. After 30 minutes the intensity was
approximately 40% of that observed initially and a second trough, not present in the spectrum
of the as-prepared enzyme, was apparent at 630 nm, Fig. 4B. After 17 hours the 620 nm
trough had been restored to 60% of its original intensity and there was no indication of
additional negative intensity at longer wavelengths. The transient appearance of a trough at
630 nm on re-oxidation of TvNiR suggests a Lys/water ligated high-spin ferric heme (34) as
an intermediate in the pathway of oxidizing the high-spin ferrous heme to its
thermodynamically most stable Lys/hydroxide ligated ferric state.
TvNiR nitrite reductase activity resolved by protein film electrochemistry and compared to
that of EcNrfA – PFE was used to resolve the catalytic activity of TvNiR and EcNrfA as a
function of pH. For this the mesoporous nanocrystalline SnO2 electrodes were unsuitable.
Substrate mass transport to enzyme molecules adsorbed deep within the mesoporous structure
limits the catalytic current and masks the intrinsic properties of the enzyme (35). Instead both
enzymes were adsorbed on PGE electrodes that were rotated rapidly to further facilitate
substrate delivery to the electrode surface. Cyclic voltammetry of freshly polished PGE
electrodes exposed to a few microlitres of TvNiR, placed in 0.1 mM nitrite and rotated at
3000 rpm gave rise to negatively signed, and so reductive, catalytic currents below -100 mV,
Fig. 5A. The catalytic response did not change on addition of 100 mM phosphate but it was
lost on introducing 500 µM cyanide, not shown. (Note: cyanide is toxic and extreme care
should be taken in its handling and disposal.) These observations correlate with the catalytic
properties of TvNiR resolved by spectrophotometric assays using dithionite reduced methyl
viologen as electron donor (22). It was concluded that TvNiR, like EcNrfA (20,36), adsorbs
on PGE electrodes in an electrocatalytically active form that retains the characteristic
properties of the enzyme in solution. Non-turnover peaks whose area would quantify the
amount of electroactive enzyme were not visible for either enzyme and this is most likely
related to poor surface coverage. This includes the possibility of a distribution of orientations
on the surface many of which are incapable of direct electron exchange with the electrode
and it prevents the calculation of turnover numbers for nitrite reduction. Nevertheless the
variation in catalytic current with electrochemical potential, time and pH provides much
insight into the catalytic performance of the enzymes as described below.
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The catalytic properties of TvNiR are more apparent when the voltammetric response of
the bare electrode is subtracted from that displayed by the enzyme film under otherwise
identical conditions, Fig. 5B. The catalytic current magnitudes of the first reductive scan from
+200 to -600 mV, Fig. 5B bold continuous line, are smaller than those resolved on returning
to more positive potentials, Fig. 5B thin continuous line. The catalytic current-potential
profiles of the subsequent cyclic voltammograms do not show this hysteresis, Fig. 5B broken
lines. The profiles for cyclic voltammograms 2 to 5 are independent of scan direction aside
from a first order loss of catalytic current magnitude over time that is typical of such
experiments and attributed to loss of electrocatalytically active enzyme. Significantly the
catalytic current-potential profiles of scans 2 to 5 resemble that seen on returning to positive
potentials during the first voltammogram. Taken together the cyclic voltammetry describes an
irreversible reductive activation, on the time scale of the PFE experiment, of the TvNiR film
on scanning from +200 to -600 mV for the first time. Such behavior has not been noted for
EcNrfA. The irreversible reductive activation is distinct from the reversible modulation of
activity that is observed on subsequent cycling of the enzyme from more positive to more
negative potentials. The latter is expected as the enzyme is swept between the oxidized,
catalytically inactive, and reduced, catalytically active, oxidation states.
Chronoamperometry was employed to resolve the rate of irreversible reductive activation
as a function of electrochemical potential, pH and nitrite concentration. This has the
advantage of avoiding the intimate linkage of time and electrochemical potential that is
intrinsic to cyclic voltammetry. The electrode potential was stepped between defined values
and the subsequent relaxation of catalytic current reported on the rate of any reductive
activation that occurred. A typical result is illustrated in Fig. 6A. Initiating the experiment at
+140 mV with the electrode rotating rapidly in 0.1 mM nitrite there is little flow of current as
expected from cyclic voltammetry, Fig. 5. The small drop of current noted in the first 20
seconds arises from charging of the electrical double-layer. On stepping the potential to -160
mV there is an immediate and significant increase in the catalytic current as anticipated from
cyclic voltammetry, e.g. Fig. 5. The current due to double-layer charging decays over 10
seconds after which time a constant catalytic current is measured. Stepping the potential to -
270 mV elicits an immediate increase in catalytic current, again as expected from the cyclic
voltammetry, followed by a further significant increase in the catalytic current over 120
seconds that describes the reductive activation of the protein film. Returning the electrode
potential to -160 mV shows the catalytic current had increased by approximately 30% from
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that prior to reductive activation. Equilibrating the enzyme at -310 mV and returning to -160
mV provided no evidence for further activation of the film.
In the experiment described above the reductive activation of TvNiR at -270 mV is well-
described as a first order process with a rate constant of 0.030 ± 0.005 s -1, Fig. 6A circles.
Reductive activation was studied in a similar manner for a number of potentials in the pH
range 6 to 10. Regardless of conditions, complete activation of the film produced a 30%
increase of activity. Reductive activation was too fast to quantify when the rate constant
exceeded 0.7 s-1 but the data clearly demonstrated how for a given potential the reductive
activation was faster at more acidic pH, Fig. 6B. Further experiments revealed that neither the
rate nor the extent of reductive activation were dependent on nitrite concentration (not
shown). When considering the possible rate-defining event in reductive activation the process
of intramolecular electron transfer seems an unlikely candidate due to the proximity of
adjacent hemes within TvNiR (37). A simple description of the reductive activation then
involves interfacial electron transfer to an activating center followed by a chemical event that
produces the more active enzyme. In this model two limiting behaviors can be put forward to
account for the variation of reductive activation rate constant with electrochemical potential.
When the rate is defined by slow interfacial electron transfer the rate constant will increase
exponentially with overpotential as described by the Butler-Volmer equation (38). This
behavior is illustrated by the red line in Fig. 6B that has been generated using parameters,
stated in the figure legend, that produce a reasonable correlation with the data at pH 7.
However, the correlation with the data is not strong and the catalytic currents supported by
TvNiR suggest interfacial electron transfer is not rate-limiting. Taking 0.05 picomoles TvNiR
as an estimate of the upper limit of electroactive coverage based on failure to resolve non-
turnover peaks when nitrite is absent indicates that each molecule of adsorbed TvNiR
supports a minimum electron flux of 500 s-1. This is significantly greater than the rate
constants for reductive activation and consistent with solution assays that report an electron
flux through TvNiR of the order of 18 000 s-1 (10).
In the absence of rate-defining interfacial electron transfer the rate of reductive activation
will be defined by the chemical step that follows reduction of the activating center. The rate
constant will reflect the population of enzyme with the activating center reduced and vary
with electrochemical potential in a sigmoidal manner as predicted by the Nernst equation and
centered on the mid-point potential of the activating center (39). This is illustrated by the
solid black line in Fig. 6B that show best fit to the pH 7 data for an n =1 center. The lack of
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data at low potentials requires that the precise values of the parameters producing this fit are
interpreted with caution but the model better reproduces the pH 7 data than the prediction
from rate-limiting interfacial electron transfer. The pH dependence of the plots of rate
constant versus electrochemical potential indicates a positive shift of Em for the activating
center with decrease of pH and so a reduction that is accompanied by protonation.
To define the KM and maximum catalytic current (imax, that is equivalent to Vmax) describing
steady-state nitrite reduction by TvNiR, nitrite was titrated into the electrochemical cell after
two cyclic voltammograms had been measured to ensure complete activation of the enzyme
film, e.g. Fig. 5 and 7. Parallel experiments were performed in solutions having a pH value
between 5 and 10. Visual inspection of the voltammograms presented in Fig. 7 readily
illustrates how lower values of imax and KM are displayed at more positive potentials. In this
work analysis was performed at -550 mV to facilitate comparison of the catalytic properties
of the adsorbed enzymes with those displayed in solution phase spectrophotometric assays
with methyl viologen (Em,7 -550 mV) as electron donor (5,10). At each pH the variation of
catalytic current magnitude with nitrite concentration was well-described by the Michaelis-
Menten equation, e.g., Fig 6 inset.
Nitrite reduction by TvNiR at pH 7 was described by a KM of 223 ± 60 µM that is in good
agreement with the value of 180 ± 50 µM determined by spectrophotometric assay (10). The
value of imax decreased in an approximately linear fashion, Fig. 8A, as the pH increased while
that of KM decreased 10-fold in an approximately linear manner, Fig. 8B. Neither behavior is
readily explained by the sigmoidal variation with pH that is expected when the protonation
state of a single ionizable residue modulates these parameters. A series of parallel
experiments established that the nitrite reductase activity of EcNrfA follows Michaelis-
Menten kinetics over this pH range (20). The pH dependence of the maximum activity of
EcNrfA is very similar to that displayed by TvNiR, Fig. 8A. However, the pH dependence of
KM is different. For EcNrfA the pH dependence of KM is well described by the behavior
expected for ionization of a single residue with pKa = 7.6 ± 0.3 where the protonated residue
results in lower values of KM.
Discussion.
Nitrite ammonification is a complex process and one for which the molecular details of the
catalytic cycle remain to be fully understood. In this context it is striking that TvNiR and
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EcNrfA differ in aspects of their catalytic behavior despite their structural similarities. That
His, Tyr and Arg are conserved in the distal pocket of the active sites of TvNiR, EcNrfA and
their homologs indicates a critical role for each of these residues in catalysis. X-ray
crystallography provides evidence for the residues that are important in binding nitrite. The
structure of EcNrfA is very similar to that of Wolinella succinogenes NrfA (WsNrfA) and the
latter enzyme has been co-crystallized with nitrite (40). One nitrite oxygen lies within H-
bonding distance of the N atom of His and the second oxygen within H-bonding distance of
the N2 atom of Arg. The Tyr OH is outside H-bonding distance of the closest nitrite oxygen
and density functional theory supports key roles for His and Arg, but not Tyr, in nitrite
binding (11). A pKa ≈ 8.2 was previously assigned to the active site His in Desulfovibrio
desulfuricans NrfA (16). If the equivalent His in EcNrfA is deprotonated with a pKa 7.6
this can account for the pH dependence of this enzyme’s KM that can be simply considered to
reflect the affinity of the active site for nitrite. His deprotonation will remove an opportunity
for H-bonding and lower the positive charge in the active site. Both effects will lower the
affinity for nitrite and increase the KM describing nitrite reduction by EcNrfA. Such a model
does not account for the pH dependence of the KM describing TvNiR nitrite reduction.
The active site of TvNiR is smaller than that of EcNrfA and it binds nitrite through H-
bonds from His and Tyr OH to one oxygen and from Arg to the second oxygen (9). This may
result in differences in the absolute affinities of TvNiR and EcNrfA for nitrite. However, it
does not readily explain why the KM for TvNiR nitrite reduction increases as the pH drops.
Within the active site of TvNiR the His is expected to contribute to catalysis in much the
same way as the equivalent residue in EcNrfA. That contribution to the mechanism must be
overridden in terms of defining the KM for TvNiR nitrite reduction if we accept that His
(de-)protonation defines the pH dependence of the KM displayed by EcNrfA. In TvNiR the KM
must instead have defining contributions from rates associated with the transformation of
catalytic intermediates and/or the microscopic steps in substrate delivery to the active site).
Differences in the structures of the two enzymes point to the features that will be responsible
for this. One is the covalent modification by Cys of the active site Tyr in TvNiR (9). This will
lower the Tyr reduction potential by up to 0.4 V but as yet there is no evidence that a Tyr
radical contributes to catalysis (4,12). Since replacing Tyr with Phe produces an enzyme
unable to reduce nitrite to ammonium (41) a key role for this residue in supplying protons,
directing nitrite into the active site or releasing reaction products must then be inferred. In
each of these functions lowering of the Tyr pKa by up to 1 unit on covalent modification may
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account for their different rates within EcNrfA and TvNiR and, as a consequence, the
different pH dependences of their KM values.
There are further differences between the two enzymes when the details of the two
channels linking their deeply buried active sites to the bulk solution are considered. These
will no doubt contribute to different rates for the microscopic steps in delivering nitrite to the
active site but whose calculation is beyond the scope of this study. However, one feature of
these channels does warrant comment. In EcNrfA a positively charged channel directs
substrate to the active site and a negatively charged channel allows for product egress. In
TvNiR it is striking that both channels have positive charge at the protein surface. This is
reminiscent of the charge distribution in the nitrite channel NirC that spans the inner
membrane of Salmonella typhimurium (42) such that nitrite may have opportunity to explore
the two channels of TvNiR than just a single channel of EcNrfA.
The catalytic properties of TvNiR and EcNrfA are further distinguished by the reductive
activation displayed by the former enzyme. While the active site of TvNiR is purified with
His/hydroxide ligation it is unlikely that this species appears in the catalytic cycle since it is
formed over a time-scale of hours following reduction and reoxidation of the enzyme. The
high-spin ferrous heme in TvNiR is most likely to be penta-coordinate (43) and it may well
re-oxidise in this state such that penta-coordinate species key to catalysis. A penta-coordinate,
high-spin heme with Lys ligation would have a LMCT band at 660 nm. Such features have
significantly lower intensity than those from the hexa-coordinate counterparts (see (44) and
references therein) and as a consequence it is not surprising that it goes undetected in our
experiments. However, this hypothesis suggests that reductive activation is achieved by
preventing hydroxide ligation of the active site heme in its ferric state. This is not a direct
effect since reduction of the Lys/hydroxide ligated heme occurs at potentials that are more
positive than those required to see reductive activation, Fig. 6B. Rather reduction of low-spin
heme(s) is responsible for this effect. The low-spin heme adjacent to the active site heme is
axially coordinated by the His that is two residues from the Tyr of the distal pocket, Fig. 9.
Movement of this His on reduction of the low-spin heme may alter the disposition of residues
in the active site distal pocket to slow the rate of hydroxide binding.
Given the discussion above it is striking that in other regards the catalytic properties of
TvNiR and EcNrfA are so similar, namely, the pH dependence of Vmax and the nitrite
dependence of the catalytic current-potential profiles resolved by cyclic voltammetry. The
determinants of these properties the enzymes appear to be conserved. Detailed discussion of
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the possible origin of the catalytic current-potential profiles has been presented previously for
EcNrfA (13,19,20,45). The pH dependence of Vmax may reflect the rate of supplying protons
to the active site and/or the roles of multiple residues in delivering the eight protons that are
required to transform nitrite to ammonium. In the future it is hoped that studies of the pH
dependence of the transformation of nitric oxide and hydroxylamine, proposed intermediates
in nitrite reduction, together with engineered variants of both enzymes will shed greater
insight into the molecular origins of each of the properties described here. Such studies may
also help to elucidate the degree to which the catalytic properties of these enzymes arise from
the environments colonized by the microbes from which they originate. Both enzymes are
periplasmic so they will experience the pH of these environments. For TvNiR this will be pH
10 of the hypersaline alkaline lakes of Wadi Natrun (Egypt) (46). For EcNrfA this will range
from pH 2 to 9 during colonization of the gastrointestinal tract. This may have resulted in
EcNrfA developing the ability to scavenge, and reduce, nitrite at low pH a feature not needed
by TvNiR.
In closing we note that the results presented here for TvNiR illustrate the difficulties that
can be experienced in using (magneto-)optical methods to resolve redox properties of a high-
spin c-heme when low-spin c-hemes are present. It has already been mentioned that the
signals from the former are of intrinsically lower intensity than those from the later.
Nevertheless it should be possible to resolve the Em of the high-spin heme if it is sufficiently
separated from those of the low-spin hemes as for EcNrfA (15). In PFE the potential of the
onset of catalysis detected during cyclic voltammetry at low substrate concentration is often
directly related to the active site reduction potential (19). On this basis the Em of the
catalytically relevant ferric/ferrous couple of the TvNiR high-spin active site heme is likely to
lie a little below -0.1 V, in the potential window spanned by redox transformation of its low-
spin hemes, and so to have a value similar to that of the active site heme in EcNrfA. This is
consistent with reduction of the active site occurring only when other hemes are reduced such
that nitrite binding may be prevented until sufficient electrons are present to avoid release of
the as yet undetected intermediates in nitrite ammonification. The Em values of the active
sites in both enzymes would also then be consistent with their ability to reduce nitrite to
ammonium and overcoming the reduction potentials associated with the proposed
intermediates in the reaction pathway; nitrite/nitric oxide (ca. +0.18 V), nitric
oxide/hydroxylamine (ca. -0.06 V) and hydroxylamine/ammonium (ca. +0.87 V). By contrast
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potentials a little below +0.3 V are sufficient to detect nitrite reduction to form nitric oxide by
the copper-containing enzyme from Alcaligenes faecalis (47).
Acknowledgements - We are grateful to Professor James Durrant and Li Xiaoe (Imperial
College London) for providing the SnO2 electrodes used in this work and to the reviewers for
their insights into the interpretation of the data presented in the manuscript. The work was
funded by the UK Biotechnology and Biosciences Research Council through grants B15211,
C007808, B18695, G024758 and G009228.
References
1. Richardson DJ, Watmough NJ (1999) Curr Opin Chem Biol 3:207-219
2. Martinez-Espinosa R M, Cole JA, Richardson DJ, Watmough NJ (2011) Biochem Soc
Trans 39:175-178
3. Einsle O, Messerschmidt A, Stach P, Bourenkov GP, Bartunik HD, Huber R, Kroneck
P (1999) Nature 400:476-480
4. Einsle O, Stach P, Messerschmidt A, Simon J, Kroger A, Huber R, Kroneck PMH
(2000) J Biol Chem 275:39608-39616
5. Bamford VA, Angove HC, Seward HE, Thomson AJ, Cole JA, Butt JN, Hemmings
AM, Richardson DJ (2002) Biochemistry 41: 2921-2931
6. Cunha CA, Macieira S, Dias JM, Almeida G, Goncalves LL, Costa C, Lampreia J,
Huber R, Moura JJG, Moura I, Romao MJ (2003) J Biol Chem 278:17455-17465
7. Rodrigues ML, Oliveira TF, Pereira IAC, Archer M (2006) EMBO J 25:5951-5960
8. Youngblut M, Judd ET, Srajer V, Sayyed B, Goelzer T, Elliott SJ, Schmidt M,
Pacheco, AA (2012) J Biol Inorg Chem 17:647-662
9. Polyakov KM, Boyko KM, Tikhonova TV, Slutsky A, Antipov AN, Zvyagilskaya
RA, Popov AN, Bourenkov GP, Lamzin VS, Popov VO (2009) J Mol Biol 389:846-
862
10. Tikhonova T, Tikhonov A, Trofimov A, Polyakov K, Boyko K, Cherkashin E,
Rakitina T, Sorokin D, Popov V (2012) FEBS J 279:4052-4061
11. Bykov D, Neese F (2011) J Biol Inorg Chem 16:417-430
12. Bykov D,Neese F (2012) J Biol Inorg Chem 17:741-760
13. Judd ET, Youngblut M, Pacheco AA, Elliott SJ (2012) Biochemistry 51:10175-10185
18
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
Page 19
14. Almeida MG, Macieira S, Goncalves LL, Huber R, Cunha CA, Romao MJ, Costa C,
Lampreia J, Moura JJG, Moura I (2003) Eur J Biochem 270:3904-3915
15. Marritt SJ, Kemp GL, Xiaoe L, Durrant JR, Cheesman MR, Butt JN (2008) J Am
Chem Soc 130:8588-8589
16. Almeida MG, Silveira CM, Guigliarelli B, Bertrand P, Moura JJG, Moura I, Leger C
(2007) FEBS Lett 581:284-288
17. Todorovic S, Rodrigues ML, Matos D, Pereira IAC (2012) J Phys Chem B 116:5637-
5643
18. Kemp GL, Clarke TA, Marritt SJ, Lockwood C, Poock SR, Hemmings AM,
Richardson DJ, Cheesman, Butt JN (2010) Biochem J 431:73-80
19. Leger C, Bertrand P (2008) Chem Rev 108:2379-2438
20. Gwyer JD, Richardson DJ, Butt JN (2005) J Am Chem Soc 127:14964-14965
21. van Wonderen JH, Burlat B, Richardson DJ, Cheesman MR, Butt JN (2008) J Biol
Chem 283:9587-9594
22. Tikhonova TV, Slutsky A, Antipov AN, Boyko KM, Polyakov KM, Sorokin DY,
Zvyagilskaya RA, Popov VO (2006) Biochim et Biophys Acta 1764:715-723
23. Berry EA, Trumpower BL (1987) Anal Biochem 161:1-15
24. Butt WD, Keilin D (1962) Proc Roy Soc B 156:429-458
25. Vincent KA, Tilley GJ, Quammie NC, Streeter I, Burgess BK, Cheesman MR,
Armstrong F A (2003) Chem Commun 2590-2591
26. Marritt SJ, McMillan DGG, Shi L, Fredrickson JK, Zachara J, Richardson DJ, Jeuken
LJC, Butt JN (2012) Biochem Soc Trans 40:1217-1221
27. Cheesman MR, Watmough NJ, Gennis RB, Greenwood C, Thomson AJ (1994) Eur J
Biochem 219:595-602
28. Thomson AJ, Cheesman MR, George SJ (1993) Metallobiochemistry, Part C
226:199-232
29. Gadsby PMA, Thomson AJ (1990) J Am Chem Soc 112:5003-5011
30. Cheesman MR, Greenwood C, Thomson AJ (1991) Adv Inorg Chem 36:201-255
31. Astuti Y, Topoglidis E, Briscoe PB, Fantuzzi A, Gilardi G, Durrant JR (2004) J Am
Chem Soc 126:8001-8009
32. Astuti Y, Topoglidis E, Gilardi G, Durrant JR (2004) Bioelectrochemistry 63:55-59
33. Marritt SJ, Lowe TG, Bye J, McMillan DGG, Shi L, Fredrickson J, Zachara J,
Richardson DJ, Cheesman MR, Jeuken LJC, Butt JN (2012) Biochem J 444:465-474
19
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575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
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596
597
598
599
600
601
602
603
604
605
606
Page 20
34. Burlat B, Gwyer JD, Poock S, Clarke T, Cole JA, Hemmings AM, Cheesman MR,
Butt JN, Richardson DJ (2005) Biochem Soc Trans 33:137-140
35. McMillan DGG, Marritt SJ, Kemp GL, Gordon-Brown P, Butt JN, Jeuken LJC
(2013 ) Electrochim Acta in press
36. Gwyer JD, Angove HC, Richardson DJ, Butt JN (2004) Bioelectrochemistry 63:43-47
37. Page CC, Moser CC, Chen XX, Dutton PL (1999) Nature 402:47-52
38. Bard AJ, Faulkner LR (2001) Electrochemical Methods. Fundamentals and
Applications. 2nd Edition, John Wiley and Sons, Inc, New York
39. Fourmond V, Burlat B, Dementin S, Arnoux P, Sabaty M, Boiry S, Guigliarelli B,
Bertrand P, Pignol D, Leger C (2008) J Phys Chem B 112:15478-15486
40. Einsle O, Messerschmidt A, Huber R, Kroneck PMH, Neese F (2002) J Am Chem
Soc 124:11737-11745
41. Lukat P, Rudolf M, Stach P, Messerschmidt A, Kroneck PMH, Simon J, Einsle O
(2008) Biochemistry 47:2080-2086
42. Lu W, Schwarzer NJ, Du J, Gerbig-Smentek E, Andrade SLA, Einsle O (2012) Proc
Nat Acad Sci 109:18395-18400
43. Oganesyan VS, Sharonov YA (1997) Spectrochimica Acta A 53:433-449
44. McMaster, J., and Oganesyan, V. S. (2010) Curr Opin Struct Biol 20:615-622
45. Gates AJ, Kemp GL, To CY, Mann J, Marritt SJ, Mayes AG, Richardson DJ, Butt JN
(2011) Phys Chem Chem Phys 13:7720-7731
46. Sorokin DY, Antipov AN, Kuenen JG (2003) Arch Microbiol 180:127-133
47. Wijma HJ, Jeuken LJC, Verbeet MP, Armstrong FA, Canters GW (2007) J Am Chem
Soc 129:8557-8565
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Table 1 Summary of Em values for TvNiR obtained in this work and for NrfA from a number of organisms obtained in previous studies.
Protein Method Em (mV) vs SHEEscherichia coliNrfA (15)
Spectroelectrochemistry(MCD)
-20 (lsa), -108 (hsa), -153 (ls), -206 (ls), -292 (ls)
PFE(SnO2 electrode)
+22 (ls), -56 (hs), -117 (hs), -189 (hs), -275 (ls)
Shewanella oneidensis MR1NrfA (8)
Spectroelectrochemistry(electronic absorbance)
-62, -140, -250, -283, -342
PFE(graphite electrode)
-36, -105, -166, -230, -295
Desulfovibrio vulgaris NrfHA(17)
Spectroelectrochemistry(resonance Raman)
-50 (hs)
Desulfovibrio desulfuricansNrfHA (14,16)
Potentiometry b
(EPR and Mössbauer)+150 (ls), -50 (ls), -80 (hs), -480 (ls, 2 hemes)
PFE(graphite electrode)
-220 (active site CO binding heme)
Thioalkalivibrio nitratireducensTvNiR(this work)
Spectroelectrochemistry (electronic absorbance, MCD)
+100 (ls), -80 (ls), -200 (3, ls) -370 (2, ls)
a ls low-spin, hs high-spin
b Dithionite or Zn as reductant
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Fig. 1. The active sites of two nitrite ammonifying enzymes that contain CxxCK
coordinated active site hemes Ec NrfA (A) and TvNiR (B).
The catalytic triad of Tyr, His and Arg in the distal pocket are illustrated together with the
Cys modification of the active site Tyr in TvNiR. In Ec NrfA Phe218 (blue) is the equivalent
residue to Cys305 that forms a covalent link to Tyr303 in TvNiR. The Ca2+ located adjacent
to the active site is shown as a magenta sphere.
Fig. 2. Spectroscopic properties of TvNiR.
(A) Ambient-temperature MCD of oxidized TvNiR at 8 µM for 300 to 450 nm and 500 M
for 450 to 750 nm. Samples in 50 mM Hepes, 100 mM NaCl, pH 7.0. (B) Low-temperature
MCD of 70 µM oxidized TvNiR at near infra-red wavelengths measured at 4.2 K and 5 T in
25 mM Hepes, 50% glycerol D2O, pH* 7. (C) Low-temperature MCD of TvNiR reduced by
EuCl2. Spectra were measured at 5 T with the sample in 25 mM Hepes, 50% glycerol, pH 7.
Inset, electronic absorbance of oxidized TvNiR and the Eu(II) reduced enzyme prior to
freezing for MCD; y-axis ε (mM-1 cm-1). TvNiR was 11.6 M.
Fig. 3. Potentiometric titration of TvNiR adsorbed on mesoporous nanocrystalline SnO2
monitored by electronic absorption spectroscopy.
(A) Electronic absorption spectra for the enzyme equilibrated at +0.3, -0.115, -0.215, -0.315
and -0.515 V. The arrows indicate the direction of spectral changes on application of
increasingly negative potentials. Inset: Extent of reduction determined from ∆A553 nm for
reduction (●), re-oxidation (□), and re-reduction (○) where the line shows the sum of seven
Nernstian contributions of equal magnitude with Em= 0.1 (one heme), -0.08 (one heme), -0.20
(three hemes), -0.37 (two hemes) V, see text for details.
(B) Difference spectra for the indicated potentials. The vertical bar corresponds to an
absorbance difference (A) = 0.1. Buffer electrolyte was 50 mM Hepes, 100 mM NaCl, pH7.
Fig. 4. Potentiometric titration of TvNiR monitored by ambient temperature MCD.
(A) MCD of TvNiR equilibrated at increasingly negative potentials of +0.15, -0.115, -0.215, -
0.245 and -0.275 V as indicated by arrow. Inset: normalized variation of MCD intensity, 620
nm minus 634 nm, with equilibration potential in Volts and a line to guide the eye that is
centered on -230 mV. (B) MCD of TvNiR as isolated (light solid line) and following
23
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Page 24
reduction held at +180 mV for 30 minutes (heavy solid line) and 17 hrs (broken line). TvNiR
(500 M) in 50 mM Hepes, 100 mM NaCl, pH 7 with a mediator cocktail as described in the
Experimental Procedures.
Fig. 5. Cyclic voltammetry of a freshly prepared TvNiR film in 0.1 mM nitrite, pH 7.0.
(A) Continuous lines show the initial (bold) and four subsequent cyclic voltammograms from
a TvNiR film. Broken lines show the voltammograms from an equivalent experiment in the
absence of TvNiR. The arrows indicate the direction of scanning. Cyclic voltammetry was at
30 mV s-1 with electrode rotation at 3000 rpm in 20 mM Hepes, 100 mM NaCl, 20oC. (B) The
catalytic current-potential profiles of TvNiR obtained from the data in (A) as the difference
between the currents recorded in the presence and absence of TvNiR. Bold lines are for the
first cyclic voltammogram, arrows indicate scan direction. Cross-hairs indicate the zero of
current and potential.
Fig. 6. Reductive activation of TvNiR resolved by chronoamperometry.
(A) Chronoamperometry of a TvNiR film in 0.1 mM nitrite, pH 7.0. The potential applied to
the electrode for each 120 sec period is indicated. Circles show the fit to a first order increase
with a rate constant of 0.03 s-1. The catalytic current was derived from the raw data by
subtraction of the current recorded in an identical experiment performed in the absence of
TvNiR followed by first order compensation to account for the loss of signal over time. (B)
First order rate constants (s-1)for the reductive activation of TvNiR at pH 10 (star), 9 (closed
circle), 8 (square), 7 (triangle), and 6 (open circle). The black lines show the concentration of
reduced species predicted by the Nernst equation for an n =1 couple with Em = -0.42 (pH 8), -
0.36 (pH 7) and -0.27 (pH 6) V. The blue line shows the fraction of reduced active site heme
predicted by reductive titration of the 630 nm MCD trough, Fig.4A, normalized to 1.0. The
red-line illustrates the heterogeneous rate constant (cm s-1) predicted by the Butler-Volmer
equation for an n=1 process with Em = -0.28 V and ko = 0.03 cm s-1. Experimental conditions
as in Fig. 2.
Fig. 7. The steady-state nitrite reductase activity of TvNiR at pH 7.0.
(A) Steady-state catalytic currents recorded from a single film of TvNiR are shown for the
nitrite concentrations indicated. Cyclic voltammetry was at 30 mV s-1 with electrode rotation
at 3000 rpm in 20 mM Hepes, 100 mM NaCl, 20 oC. Inset: Michaelis-Menten plot derived
24
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Page 25
from the steady-state catalytic currents at -0.55 V where the line shows the best fit to the data
with KM = 0.237 mM and imax (Vmax) = 8 µA, see text for details.
Fig. 8. pH dependence of the kinetic parameters describing steady-state nitrite
reduction by TvNiR (open circles) and E. coli NrfA (closed circles).
The line in panel B describes the variation in KM for NrfA through the ionization of a single
residue with a pKa = 7.6. Buffer-electrolyte was 100 mM NaCl with 20 mM each of Hepes,
Taps, Mes and acetate for TvNiR and 2 mM CaCl2 with 25 mM each of Hepes, Mes, Taps
and acetate for E. coli NrfA. All experiments at 20 oC.
Fig. 9. The active site heme (red) and adjacent His/His ligated heme (green) in TvNiR. Two
residues separate the catalytic Tyr in the distal pocket of the active site from a His ligand of
the adjacent heme.
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Page 26
A B
Fig. 1.
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729
730
Page 27
400 500 600 700-600
-300
0
300
1000 1500 20000
1000
2000
400 6000
500
1000
400 500 600 700-15
-10
-5
0
5
10
15
(m
M-1.c
m-1)
(M
-1.c
m-1)
/H
(M-1.c
m-1.T
-1)
x10
x70
Wavelength (nm)
B J F
440 nm
427 nm
397 nm
410 nm416 nm
A
B
C
4.2 K10 K100 K
Fig. 2.
27
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732
733
Page 29
400 500 600 700
0.0
0.4
0.8
1.2
-0.4 0.0 0.4
-0.165 - (0.115)V
-0.365 - (-0.315)V
-0.415 - (-0.365)V
-0.465 - (-0.415)V-0.515 - (-0.465)V
-0.265 - (-0.215)V
-0.215 - (-0.165)V
-0.115 - (0.30)V
Red
uctio
n (%
)0
100
Potential (V)
Wavelength (nm)
Abs
orba
nce
Abs
orba
nce
Diff
eren
ceA
B
Fig. 3
29
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736
737
738
739
Page 30
600 620 640 660
-4
-2
0
-0.4 0.00
100
500 600
-40
-20
0
20
∆/H
(M-1
cm-1
T-1 )
∆/H
(M-1
cm-1
T-1 )
Wavelength (nm)
A
B
î 7
% R
educ
tion
(V)
Fig. 4.
30
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745
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747
Page 31
-0.6 -0.3 0.0
+
Potential (V) vs S.H.E.
+
1 µA
+
+
A
B
Start
Scan5
1
Scan5
1
Scan5
1
Fig. 5.
31
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750
751
752
753
Page 32
+0.14 -0.16 -0.27 -0.16 -0.31 -0.16 ElectrodePotential(V)
A
B
Cat
alyt
ic C
urre
nt (µ
A)
Time (sec)
Electrode Potential (V) vs SHE
Rat
e C
onst
ant
[Reduced] (norrm
alized)
-0.5 -0.4 -0.3 -0.2 -0.10
1
0
1
0 120 240 360 480 600 720
-4.0
-2.0
0.0
Fig. 6.
32
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758
759
Page 33
-0.6 -0.3 0.0
-4.0
-2.0
0.0
0 60
10
Cat
alyt
ic C
urre
nt (µ
A)
Potential (V) vs S.H.E.
[nitrite]mM0.01
0.08
0.16
0.32
1.282.56 [nitrite] (mM)
Cat
alyt
ic C
urre
ntM
agni
tude
(A
)
Fig. 7.
33
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765
Page 34
0.0
1.0
2.0
3.0
5 6 7 8 9 100.0
0.2
0.4
0.6
pH
KM
(mM
)i m
ax(n
orm
aliz
ed a
t pH
7) A
B
Fig. 8.
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768
769
Page 35
Fig.9.
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