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II Simposio de Fisiología y Mejora de Cereales The Zliten mosaic is a Roman floor mosaic from about the 2nd century AD, found in the town of Zliten in Libya (Roman Leptis Magna) and it is now on display at The Archaeological Museum of Tripoli . Why did Roman Gladiators eat barley? Barley, a healthy grain
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Page 1: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

The Zliten mosaic is a Roman floor mosaic from about the 2nd century AD found in the town of Zliten in Libya (Roman Leptis Magna) and it is now on display at The Archaeological Museum of Tripoli

Why did Roman Gladiators eat barley Barley a healthy grain

II Simposio de Fisiologiacutea y Mejora de Cereales

Gaius Plinius Secundus der altere (23 AD- 79 AD) (NaturalisHistoria) Barley is one of the most ancient aliments of man hellip the name of hordearii that used to be given to gladiators (barley men)

Aelius Galenus of Pergamum (130 AD ndash 210 ad) surgeon of gladiators The gladiatorsrsquo standard foods were pulse pudding and barley The pudding was often served as a mash of grain legume mixed with peeled barley sometimes it was watered down to make a soup a thick liquid drunk from pottery cups

Gladiators were slaves or criminals that suffered infamia that was the officialexclusion from the legal protections enjoyed by a Roman citizen However trainingwas expensive they were highly valued well-fed and not to be slain lightlyUnwittingly barley was key to achieve the speed strength and endurance theyneeded

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley is one of the most adaptable crops grown in subtropicaltemperate and subarctic zones from the sea level to the Andes andHimalayas

Colored grainsvrs1 6 rows Vrs1 2 rows

Nud hulled nud Hull-less Endosperm

Type Amylose Amylopectin

Normal 1 3

Waxy 05 35

High Amylose 15 25

Genetic diversity of barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Uses of Barley

Reinheitsgebot 1487 1516

II Simposio de Fisiologiacutea y Mejora de Cereales

1) Encourage diversity of varieties and cereal species 2) Genetic-selection research should focus on lsquopackagesrsquo of bioactives compounds3) Measure the impact of organic agriculture on the health potential of grain products 4) Study the effect of reducing rate of nitrogen fertilisation

A more sustainable cereal sector would preserve the environment (environmentalsustainability) and promote health (physiological sustainability) at a price that is acceptableto consumers (socio-economic sustainability) hellip

Improvement of the nutritional value of cereal products in the framework of a sustainabledevelopment should involve the concerted contribution of different actors of the sectorrdquo

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Nutritional Composition

JL Molina-Cano (1989) La cebada morfologiacutea geneacutetica agronomiacutea y usos industriales

CARBOHIDRATES 70-80

PROTEINS 9-17

LIPIDS 2-3

MINORITY COMPONETS

Dietetic fiber β-Glucans Arabinoxylans

Phenolic Compounds

Vitamins Tocopherols Tocotrienols

Micronutrients Mo Se Cu Cr P Mg

II Simposio de Fisiologiacutea y Mejora de Cereales

β (1-31-4)glucans

Content 3-12

β(1-4)

β(1-3)

Content 4-6

Arabinoxylans

Dietary (Mostly Soluble) fiber

MS Izydorczyk Canada Grain Commi

II Simposio de Fisiologiacutea y Mejora de Cereales

Material Claimed effect EFSA Journal

Beta-glucans Contribute to the maintenance of normal blood cholesterol levels2009 7(9)1254 2011 9(6)2207

Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo

Wide range of claims 2010 8(10)1735

Whole grain(WG) WG flour WG foods Diets rich in WG

Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176

Resistant starchReplacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal

2011 9(4)2024

Arabinoxylan (AX) produced from wheat endosperm

Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal

2011 9(6)2205

Beta-glucans from oats and barley

Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal

2011 9(6)2207

Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249

httpshealthgrainorg

Barley-related Health Claim

Hoja1

Hoja2

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley and Oat β-Glucan Health Claim

American Food and Drug Administration (FDA) 2006 European Food Safety Authority (EFSA) 2011Health Canada 2012

Health claim Dosis

Reduction of the postprandial glycemicresponse

4 g per 30 g of carbohidrates

Maintenance of normal levels of LDL cholesterol in blood

1 g meal 3 g day

Reduction of blood cholesterol

3 gday 1 g portion

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

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  • Phenolic Compounds
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Material Claimed effect EFSA Journal
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels 2009 7(9)1254 2011 9(6)2207
Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo Wide range of claims 2010 8(10)1735
Whole grain(WG) WG flour WG foods Diets rich in WG Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal 2011 9(4)2024
Arabinoxylan (AX) produced from wheat endosperm Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal 2011 9(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal 2011 9(6)2207
Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249
Material Claimed effect EFSA Opinion Conditions of use EFSA Journal
Barley grain fibre Contributes to an increase in faecal bulk 20119(6)2249
Arabinoxylan (AX) produced from wheat endosperm (wheat grain fibre mainly soluble) Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal May be used only for food which contains at least 8 g AX- rich fibre produced from wheat endosperm (ge 60 AX) per 100 g of available carbohydrates in a quantified portion as part of the meal 20119(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal The claim may be used only for food which contains ge 4 g of beta-glucans from oats or barley for each 30 g of available carbohydrates in a quantified portion as part of the meal In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained by consuming the beta-glucans from oats or barley as part of the meal 20119(6)2207
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels The claim may be used only for food which contains ge 1 g of beta-glucans from oats oatbran barley barley bran or from mixtures of these sources per quantified portion In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained with a daily intake of 3g 2009 7(9)1254 20119(6)2207
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal The claim may be used only for food in which digestible starch has been replaced by resistant starch so that the final content of resistant starch is at least 14 of total starch 20119(4)2024
Whole grain(WG) WG flour WG foods Diets rich in WG gut healthbowel function weight control blood glucoseinsulin levels the food constituent whole grain which is the subject of this opinion is not sufficiently characterised in relation to the 20108(10)176
Page 2: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Gaius Plinius Secundus der altere (23 AD- 79 AD) (NaturalisHistoria) Barley is one of the most ancient aliments of man hellip the name of hordearii that used to be given to gladiators (barley men)

Aelius Galenus of Pergamum (130 AD ndash 210 ad) surgeon of gladiators The gladiatorsrsquo standard foods were pulse pudding and barley The pudding was often served as a mash of grain legume mixed with peeled barley sometimes it was watered down to make a soup a thick liquid drunk from pottery cups

Gladiators were slaves or criminals that suffered infamia that was the officialexclusion from the legal protections enjoyed by a Roman citizen However trainingwas expensive they were highly valued well-fed and not to be slain lightlyUnwittingly barley was key to achieve the speed strength and endurance theyneeded

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley is one of the most adaptable crops grown in subtropicaltemperate and subarctic zones from the sea level to the Andes andHimalayas

Colored grainsvrs1 6 rows Vrs1 2 rows

Nud hulled nud Hull-less Endosperm

Type Amylose Amylopectin

Normal 1 3

Waxy 05 35

High Amylose 15 25

Genetic diversity of barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Uses of Barley

Reinheitsgebot 1487 1516

II Simposio de Fisiologiacutea y Mejora de Cereales

1) Encourage diversity of varieties and cereal species 2) Genetic-selection research should focus on lsquopackagesrsquo of bioactives compounds3) Measure the impact of organic agriculture on the health potential of grain products 4) Study the effect of reducing rate of nitrogen fertilisation

A more sustainable cereal sector would preserve the environment (environmentalsustainability) and promote health (physiological sustainability) at a price that is acceptableto consumers (socio-economic sustainability) hellip

Improvement of the nutritional value of cereal products in the framework of a sustainabledevelopment should involve the concerted contribution of different actors of the sectorrdquo

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Nutritional Composition

JL Molina-Cano (1989) La cebada morfologiacutea geneacutetica agronomiacutea y usos industriales

CARBOHIDRATES 70-80

PROTEINS 9-17

LIPIDS 2-3

MINORITY COMPONETS

Dietetic fiber β-Glucans Arabinoxylans

Phenolic Compounds

Vitamins Tocopherols Tocotrienols

Micronutrients Mo Se Cu Cr P Mg

II Simposio de Fisiologiacutea y Mejora de Cereales

β (1-31-4)glucans

Content 3-12

β(1-4)

β(1-3)

Content 4-6

Arabinoxylans

Dietary (Mostly Soluble) fiber

MS Izydorczyk Canada Grain Commi

II Simposio de Fisiologiacutea y Mejora de Cereales

Material Claimed effect EFSA Journal

Beta-glucans Contribute to the maintenance of normal blood cholesterol levels2009 7(9)1254 2011 9(6)2207

Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo

Wide range of claims 2010 8(10)1735

Whole grain(WG) WG flour WG foods Diets rich in WG

Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176

Resistant starchReplacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal

2011 9(4)2024

Arabinoxylan (AX) produced from wheat endosperm

Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal

2011 9(6)2205

Beta-glucans from oats and barley

Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal

2011 9(6)2207

Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249

httpshealthgrainorg

Barley-related Health Claim

Hoja1

Hoja2

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley and Oat β-Glucan Health Claim

American Food and Drug Administration (FDA) 2006 European Food Safety Authority (EFSA) 2011Health Canada 2012

Health claim Dosis

Reduction of the postprandial glycemicresponse

4 g per 30 g of carbohidrates

Maintenance of normal levels of LDL cholesterol in blood

1 g meal 3 g day

Reduction of blood cholesterol

3 gday 1 g portion

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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  • Phenolic Compounds
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Material Claimed effect EFSA Journal
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels 2009 7(9)1254 2011 9(6)2207
Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo Wide range of claims 2010 8(10)1735
Whole grain(WG) WG flour WG foods Diets rich in WG Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal 2011 9(4)2024
Arabinoxylan (AX) produced from wheat endosperm Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal 2011 9(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal 2011 9(6)2207
Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249
Material Claimed effect EFSA Opinion Conditions of use EFSA Journal
Barley grain fibre Contributes to an increase in faecal bulk 20119(6)2249
Arabinoxylan (AX) produced from wheat endosperm (wheat grain fibre mainly soluble) Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal May be used only for food which contains at least 8 g AX- rich fibre produced from wheat endosperm (ge 60 AX) per 100 g of available carbohydrates in a quantified portion as part of the meal 20119(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal The claim may be used only for food which contains ge 4 g of beta-glucans from oats or barley for each 30 g of available carbohydrates in a quantified portion as part of the meal In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained by consuming the beta-glucans from oats or barley as part of the meal 20119(6)2207
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels The claim may be used only for food which contains ge 1 g of beta-glucans from oats oatbran barley barley bran or from mixtures of these sources per quantified portion In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained with a daily intake of 3g 2009 7(9)1254 20119(6)2207
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal The claim may be used only for food in which digestible starch has been replaced by resistant starch so that the final content of resistant starch is at least 14 of total starch 20119(4)2024
Whole grain(WG) WG flour WG foods Diets rich in WG gut healthbowel function weight control blood glucoseinsulin levels the food constituent whole grain which is the subject of this opinion is not sufficiently characterised in relation to the 20108(10)176
Page 3: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley is one of the most adaptable crops grown in subtropicaltemperate and subarctic zones from the sea level to the Andes andHimalayas

Colored grainsvrs1 6 rows Vrs1 2 rows

Nud hulled nud Hull-less Endosperm

Type Amylose Amylopectin

Normal 1 3

Waxy 05 35

High Amylose 15 25

Genetic diversity of barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Uses of Barley

Reinheitsgebot 1487 1516

II Simposio de Fisiologiacutea y Mejora de Cereales

1) Encourage diversity of varieties and cereal species 2) Genetic-selection research should focus on lsquopackagesrsquo of bioactives compounds3) Measure the impact of organic agriculture on the health potential of grain products 4) Study the effect of reducing rate of nitrogen fertilisation

A more sustainable cereal sector would preserve the environment (environmentalsustainability) and promote health (physiological sustainability) at a price that is acceptableto consumers (socio-economic sustainability) hellip

Improvement of the nutritional value of cereal products in the framework of a sustainabledevelopment should involve the concerted contribution of different actors of the sectorrdquo

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Nutritional Composition

JL Molina-Cano (1989) La cebada morfologiacutea geneacutetica agronomiacutea y usos industriales

CARBOHIDRATES 70-80

PROTEINS 9-17

LIPIDS 2-3

MINORITY COMPONETS

Dietetic fiber β-Glucans Arabinoxylans

Phenolic Compounds

Vitamins Tocopherols Tocotrienols

Micronutrients Mo Se Cu Cr P Mg

II Simposio de Fisiologiacutea y Mejora de Cereales

β (1-31-4)glucans

Content 3-12

β(1-4)

β(1-3)

Content 4-6

Arabinoxylans

Dietary (Mostly Soluble) fiber

MS Izydorczyk Canada Grain Commi

II Simposio de Fisiologiacutea y Mejora de Cereales

Material Claimed effect EFSA Journal

Beta-glucans Contribute to the maintenance of normal blood cholesterol levels2009 7(9)1254 2011 9(6)2207

Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo

Wide range of claims 2010 8(10)1735

Whole grain(WG) WG flour WG foods Diets rich in WG

Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176

Resistant starchReplacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal

2011 9(4)2024

Arabinoxylan (AX) produced from wheat endosperm

Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal

2011 9(6)2205

Beta-glucans from oats and barley

Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal

2011 9(6)2207

Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249

httpshealthgrainorg

Barley-related Health Claim

Hoja1

Hoja2

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley and Oat β-Glucan Health Claim

American Food and Drug Administration (FDA) 2006 European Food Safety Authority (EFSA) 2011Health Canada 2012

Health claim Dosis

Reduction of the postprandial glycemicresponse

4 g per 30 g of carbohidrates

Maintenance of normal levels of LDL cholesterol in blood

1 g meal 3 g day

Reduction of blood cholesterol

3 gday 1 g portion

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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  • Phenolic Compounds
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Material Claimed effect EFSA Journal
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels 2009 7(9)1254 2011 9(6)2207
Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo Wide range of claims 2010 8(10)1735
Whole grain(WG) WG flour WG foods Diets rich in WG Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal 2011 9(4)2024
Arabinoxylan (AX) produced from wheat endosperm Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal 2011 9(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal 2011 9(6)2207
Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249
Material Claimed effect EFSA Opinion Conditions of use EFSA Journal
Barley grain fibre Contributes to an increase in faecal bulk 20119(6)2249
Arabinoxylan (AX) produced from wheat endosperm (wheat grain fibre mainly soluble) Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal May be used only for food which contains at least 8 g AX- rich fibre produced from wheat endosperm (ge 60 AX) per 100 g of available carbohydrates in a quantified portion as part of the meal 20119(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal The claim may be used only for food which contains ge 4 g of beta-glucans from oats or barley for each 30 g of available carbohydrates in a quantified portion as part of the meal In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained by consuming the beta-glucans from oats or barley as part of the meal 20119(6)2207
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels The claim may be used only for food which contains ge 1 g of beta-glucans from oats oatbran barley barley bran or from mixtures of these sources per quantified portion In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained with a daily intake of 3g 2009 7(9)1254 20119(6)2207
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal The claim may be used only for food in which digestible starch has been replaced by resistant starch so that the final content of resistant starch is at least 14 of total starch 20119(4)2024
Whole grain(WG) WG flour WG foods Diets rich in WG gut healthbowel function weight control blood glucoseinsulin levels the food constituent whole grain which is the subject of this opinion is not sufficiently characterised in relation to the 20108(10)176
Page 4: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Uses of Barley

Reinheitsgebot 1487 1516

II Simposio de Fisiologiacutea y Mejora de Cereales

1) Encourage diversity of varieties and cereal species 2) Genetic-selection research should focus on lsquopackagesrsquo of bioactives compounds3) Measure the impact of organic agriculture on the health potential of grain products 4) Study the effect of reducing rate of nitrogen fertilisation

A more sustainable cereal sector would preserve the environment (environmentalsustainability) and promote health (physiological sustainability) at a price that is acceptableto consumers (socio-economic sustainability) hellip

Improvement of the nutritional value of cereal products in the framework of a sustainabledevelopment should involve the concerted contribution of different actors of the sectorrdquo

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Nutritional Composition

JL Molina-Cano (1989) La cebada morfologiacutea geneacutetica agronomiacutea y usos industriales

CARBOHIDRATES 70-80

PROTEINS 9-17

LIPIDS 2-3

MINORITY COMPONETS

Dietetic fiber β-Glucans Arabinoxylans

Phenolic Compounds

Vitamins Tocopherols Tocotrienols

Micronutrients Mo Se Cu Cr P Mg

II Simposio de Fisiologiacutea y Mejora de Cereales

β (1-31-4)glucans

Content 3-12

β(1-4)

β(1-3)

Content 4-6

Arabinoxylans

Dietary (Mostly Soluble) fiber

MS Izydorczyk Canada Grain Commi

II Simposio de Fisiologiacutea y Mejora de Cereales

Material Claimed effect EFSA Journal

Beta-glucans Contribute to the maintenance of normal blood cholesterol levels2009 7(9)1254 2011 9(6)2207

Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo

Wide range of claims 2010 8(10)1735

Whole grain(WG) WG flour WG foods Diets rich in WG

Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176

Resistant starchReplacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal

2011 9(4)2024

Arabinoxylan (AX) produced from wheat endosperm

Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal

2011 9(6)2205

Beta-glucans from oats and barley

Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal

2011 9(6)2207

Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249

httpshealthgrainorg

Barley-related Health Claim

Hoja1

Hoja2

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley and Oat β-Glucan Health Claim

American Food and Drug Administration (FDA) 2006 European Food Safety Authority (EFSA) 2011Health Canada 2012

Health claim Dosis

Reduction of the postprandial glycemicresponse

4 g per 30 g of carbohidrates

Maintenance of normal levels of LDL cholesterol in blood

1 g meal 3 g day

Reduction of blood cholesterol

3 gday 1 g portion

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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  • Phenolic Compounds
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Material Claimed effect EFSA Journal
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels 2009 7(9)1254 2011 9(6)2207
Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo Wide range of claims 2010 8(10)1735
Whole grain(WG) WG flour WG foods Diets rich in WG Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal 2011 9(4)2024
Arabinoxylan (AX) produced from wheat endosperm Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal 2011 9(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal 2011 9(6)2207
Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249
Material Claimed effect EFSA Opinion Conditions of use EFSA Journal
Barley grain fibre Contributes to an increase in faecal bulk 20119(6)2249
Arabinoxylan (AX) produced from wheat endosperm (wheat grain fibre mainly soluble) Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal May be used only for food which contains at least 8 g AX- rich fibre produced from wheat endosperm (ge 60 AX) per 100 g of available carbohydrates in a quantified portion as part of the meal 20119(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal The claim may be used only for food which contains ge 4 g of beta-glucans from oats or barley for each 30 g of available carbohydrates in a quantified portion as part of the meal In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained by consuming the beta-glucans from oats or barley as part of the meal 20119(6)2207
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels The claim may be used only for food which contains ge 1 g of beta-glucans from oats oatbran barley barley bran or from mixtures of these sources per quantified portion In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained with a daily intake of 3g 2009 7(9)1254 20119(6)2207
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal The claim may be used only for food in which digestible starch has been replaced by resistant starch so that the final content of resistant starch is at least 14 of total starch 20119(4)2024
Whole grain(WG) WG flour WG foods Diets rich in WG gut healthbowel function weight control blood glucoseinsulin levels the food constituent whole grain which is the subject of this opinion is not sufficiently characterised in relation to the 20108(10)176
Page 5: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

1) Encourage diversity of varieties and cereal species 2) Genetic-selection research should focus on lsquopackagesrsquo of bioactives compounds3) Measure the impact of organic agriculture on the health potential of grain products 4) Study the effect of reducing rate of nitrogen fertilisation

A more sustainable cereal sector would preserve the environment (environmentalsustainability) and promote health (physiological sustainability) at a price that is acceptableto consumers (socio-economic sustainability) hellip

Improvement of the nutritional value of cereal products in the framework of a sustainabledevelopment should involve the concerted contribution of different actors of the sectorrdquo

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Nutritional Composition

JL Molina-Cano (1989) La cebada morfologiacutea geneacutetica agronomiacutea y usos industriales

CARBOHIDRATES 70-80

PROTEINS 9-17

LIPIDS 2-3

MINORITY COMPONETS

Dietetic fiber β-Glucans Arabinoxylans

Phenolic Compounds

Vitamins Tocopherols Tocotrienols

Micronutrients Mo Se Cu Cr P Mg

II Simposio de Fisiologiacutea y Mejora de Cereales

β (1-31-4)glucans

Content 3-12

β(1-4)

β(1-3)

Content 4-6

Arabinoxylans

Dietary (Mostly Soluble) fiber

MS Izydorczyk Canada Grain Commi

II Simposio de Fisiologiacutea y Mejora de Cereales

Material Claimed effect EFSA Journal

Beta-glucans Contribute to the maintenance of normal blood cholesterol levels2009 7(9)1254 2011 9(6)2207

Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo

Wide range of claims 2010 8(10)1735

Whole grain(WG) WG flour WG foods Diets rich in WG

Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176

Resistant starchReplacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal

2011 9(4)2024

Arabinoxylan (AX) produced from wheat endosperm

Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal

2011 9(6)2205

Beta-glucans from oats and barley

Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal

2011 9(6)2207

Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249

httpshealthgrainorg

Barley-related Health Claim

Hoja1

Hoja2

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley and Oat β-Glucan Health Claim

American Food and Drug Administration (FDA) 2006 European Food Safety Authority (EFSA) 2011Health Canada 2012

Health claim Dosis

Reduction of the postprandial glycemicresponse

4 g per 30 g of carbohidrates

Maintenance of normal levels of LDL cholesterol in blood

1 g meal 3 g day

Reduction of blood cholesterol

3 gday 1 g portion

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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  • Phenolic Compounds
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Material Claimed effect EFSA Journal
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels 2009 7(9)1254 2011 9(6)2207
Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo Wide range of claims 2010 8(10)1735
Whole grain(WG) WG flour WG foods Diets rich in WG Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal 2011 9(4)2024
Arabinoxylan (AX) produced from wheat endosperm Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal 2011 9(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal 2011 9(6)2207
Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249
Material Claimed effect EFSA Opinion Conditions of use EFSA Journal
Barley grain fibre Contributes to an increase in faecal bulk 20119(6)2249
Arabinoxylan (AX) produced from wheat endosperm (wheat grain fibre mainly soluble) Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal May be used only for food which contains at least 8 g AX- rich fibre produced from wheat endosperm (ge 60 AX) per 100 g of available carbohydrates in a quantified portion as part of the meal 20119(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal The claim may be used only for food which contains ge 4 g of beta-glucans from oats or barley for each 30 g of available carbohydrates in a quantified portion as part of the meal In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained by consuming the beta-glucans from oats or barley as part of the meal 20119(6)2207
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels The claim may be used only for food which contains ge 1 g of beta-glucans from oats oatbran barley barley bran or from mixtures of these sources per quantified portion In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained with a daily intake of 3g 2009 7(9)1254 20119(6)2207
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal The claim may be used only for food in which digestible starch has been replaced by resistant starch so that the final content of resistant starch is at least 14 of total starch 20119(4)2024
Whole grain(WG) WG flour WG foods Diets rich in WG gut healthbowel function weight control blood glucoseinsulin levels the food constituent whole grain which is the subject of this opinion is not sufficiently characterised in relation to the 20108(10)176
Page 6: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Nutritional Composition

JL Molina-Cano (1989) La cebada morfologiacutea geneacutetica agronomiacutea y usos industriales

CARBOHIDRATES 70-80

PROTEINS 9-17

LIPIDS 2-3

MINORITY COMPONETS

Dietetic fiber β-Glucans Arabinoxylans

Phenolic Compounds

Vitamins Tocopherols Tocotrienols

Micronutrients Mo Se Cu Cr P Mg

II Simposio de Fisiologiacutea y Mejora de Cereales

β (1-31-4)glucans

Content 3-12

β(1-4)

β(1-3)

Content 4-6

Arabinoxylans

Dietary (Mostly Soluble) fiber

MS Izydorczyk Canada Grain Commi

II Simposio de Fisiologiacutea y Mejora de Cereales

Material Claimed effect EFSA Journal

Beta-glucans Contribute to the maintenance of normal blood cholesterol levels2009 7(9)1254 2011 9(6)2207

Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo

Wide range of claims 2010 8(10)1735

Whole grain(WG) WG flour WG foods Diets rich in WG

Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176

Resistant starchReplacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal

2011 9(4)2024

Arabinoxylan (AX) produced from wheat endosperm

Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal

2011 9(6)2205

Beta-glucans from oats and barley

Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal

2011 9(6)2207

Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249

httpshealthgrainorg

Barley-related Health Claim

Hoja1

Hoja2

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley and Oat β-Glucan Health Claim

American Food and Drug Administration (FDA) 2006 European Food Safety Authority (EFSA) 2011Health Canada 2012

Health claim Dosis

Reduction of the postprandial glycemicresponse

4 g per 30 g of carbohidrates

Maintenance of normal levels of LDL cholesterol in blood

1 g meal 3 g day

Reduction of blood cholesterol

3 gday 1 g portion

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Material Claimed effect EFSA Journal
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels 2009 7(9)1254 2011 9(6)2207
Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo Wide range of claims 2010 8(10)1735
Whole grain(WG) WG flour WG foods Diets rich in WG Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal 2011 9(4)2024
Arabinoxylan (AX) produced from wheat endosperm Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal 2011 9(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal 2011 9(6)2207
Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249
Material Claimed effect EFSA Opinion Conditions of use EFSA Journal
Barley grain fibre Contributes to an increase in faecal bulk 20119(6)2249
Arabinoxylan (AX) produced from wheat endosperm (wheat grain fibre mainly soluble) Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal May be used only for food which contains at least 8 g AX- rich fibre produced from wheat endosperm (ge 60 AX) per 100 g of available carbohydrates in a quantified portion as part of the meal 20119(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal The claim may be used only for food which contains ge 4 g of beta-glucans from oats or barley for each 30 g of available carbohydrates in a quantified portion as part of the meal In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained by consuming the beta-glucans from oats or barley as part of the meal 20119(6)2207
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels The claim may be used only for food which contains ge 1 g of beta-glucans from oats oatbran barley barley bran or from mixtures of these sources per quantified portion In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained with a daily intake of 3g 2009 7(9)1254 20119(6)2207
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal The claim may be used only for food in which digestible starch has been replaced by resistant starch so that the final content of resistant starch is at least 14 of total starch 20119(4)2024
Whole grain(WG) WG flour WG foods Diets rich in WG gut healthbowel function weight control blood glucoseinsulin levels the food constituent whole grain which is the subject of this opinion is not sufficiently characterised in relation to the 20108(10)176
Page 7: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Nutritional Composition

JL Molina-Cano (1989) La cebada morfologiacutea geneacutetica agronomiacutea y usos industriales

CARBOHIDRATES 70-80

PROTEINS 9-17

LIPIDS 2-3

MINORITY COMPONETS

Dietetic fiber β-Glucans Arabinoxylans

Phenolic Compounds

Vitamins Tocopherols Tocotrienols

Micronutrients Mo Se Cu Cr P Mg

II Simposio de Fisiologiacutea y Mejora de Cereales

β (1-31-4)glucans

Content 3-12

β(1-4)

β(1-3)

Content 4-6

Arabinoxylans

Dietary (Mostly Soluble) fiber

MS Izydorczyk Canada Grain Commi

II Simposio de Fisiologiacutea y Mejora de Cereales

Material Claimed effect EFSA Journal

Beta-glucans Contribute to the maintenance of normal blood cholesterol levels2009 7(9)1254 2011 9(6)2207

Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo

Wide range of claims 2010 8(10)1735

Whole grain(WG) WG flour WG foods Diets rich in WG

Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176

Resistant starchReplacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal

2011 9(4)2024

Arabinoxylan (AX) produced from wheat endosperm

Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal

2011 9(6)2205

Beta-glucans from oats and barley

Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal

2011 9(6)2207

Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249

httpshealthgrainorg

Barley-related Health Claim

Hoja1

Hoja2

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley and Oat β-Glucan Health Claim

American Food and Drug Administration (FDA) 2006 European Food Safety Authority (EFSA) 2011Health Canada 2012

Health claim Dosis

Reduction of the postprandial glycemicresponse

4 g per 30 g of carbohidrates

Maintenance of normal levels of LDL cholesterol in blood

1 g meal 3 g day

Reduction of blood cholesterol

3 gday 1 g portion

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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  • Phenolic Compounds
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  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Material Claimed effect EFSA Journal
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels 2009 7(9)1254 2011 9(6)2207
Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo Wide range of claims 2010 8(10)1735
Whole grain(WG) WG flour WG foods Diets rich in WG Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal 2011 9(4)2024
Arabinoxylan (AX) produced from wheat endosperm Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal 2011 9(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal 2011 9(6)2207
Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249
Material Claimed effect EFSA Opinion Conditions of use EFSA Journal
Barley grain fibre Contributes to an increase in faecal bulk 20119(6)2249
Arabinoxylan (AX) produced from wheat endosperm (wheat grain fibre mainly soluble) Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal May be used only for food which contains at least 8 g AX- rich fibre produced from wheat endosperm (ge 60 AX) per 100 g of available carbohydrates in a quantified portion as part of the meal 20119(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal The claim may be used only for food which contains ge 4 g of beta-glucans from oats or barley for each 30 g of available carbohydrates in a quantified portion as part of the meal In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained by consuming the beta-glucans from oats or barley as part of the meal 20119(6)2207
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels The claim may be used only for food which contains ge 1 g of beta-glucans from oats oatbran barley barley bran or from mixtures of these sources per quantified portion In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained with a daily intake of 3g 2009 7(9)1254 20119(6)2207
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal The claim may be used only for food in which digestible starch has been replaced by resistant starch so that the final content of resistant starch is at least 14 of total starch 20119(4)2024
Whole grain(WG) WG flour WG foods Diets rich in WG gut healthbowel function weight control blood glucoseinsulin levels the food constituent whole grain which is the subject of this opinion is not sufficiently characterised in relation to the 20108(10)176
Page 8: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Nutritional Composition

JL Molina-Cano (1989) La cebada morfologiacutea geneacutetica agronomiacutea y usos industriales

CARBOHIDRATES 70-80

PROTEINS 9-17

LIPIDS 2-3

MINORITY COMPONETS

Dietetic fiber β-Glucans Arabinoxylans

Phenolic Compounds

Vitamins Tocopherols Tocotrienols

Micronutrients Mo Se Cu Cr P Mg

II Simposio de Fisiologiacutea y Mejora de Cereales

β (1-31-4)glucans

Content 3-12

β(1-4)

β(1-3)

Content 4-6

Arabinoxylans

Dietary (Mostly Soluble) fiber

MS Izydorczyk Canada Grain Commi

II Simposio de Fisiologiacutea y Mejora de Cereales

Material Claimed effect EFSA Journal

Beta-glucans Contribute to the maintenance of normal blood cholesterol levels2009 7(9)1254 2011 9(6)2207

Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo

Wide range of claims 2010 8(10)1735

Whole grain(WG) WG flour WG foods Diets rich in WG

Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176

Resistant starchReplacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal

2011 9(4)2024

Arabinoxylan (AX) produced from wheat endosperm

Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal

2011 9(6)2205

Beta-glucans from oats and barley

Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal

2011 9(6)2207

Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249

httpshealthgrainorg

Barley-related Health Claim

Hoja1

Hoja2

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley and Oat β-Glucan Health Claim

American Food and Drug Administration (FDA) 2006 European Food Safety Authority (EFSA) 2011Health Canada 2012

Health claim Dosis

Reduction of the postprandial glycemicresponse

4 g per 30 g of carbohidrates

Maintenance of normal levels of LDL cholesterol in blood

1 g meal 3 g day

Reduction of blood cholesterol

3 gday 1 g portion

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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  • Phenolic Compounds
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Material Claimed effect EFSA Journal
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels 2009 7(9)1254 2011 9(6)2207
Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo Wide range of claims 2010 8(10)1735
Whole grain(WG) WG flour WG foods Diets rich in WG Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal 2011 9(4)2024
Arabinoxylan (AX) produced from wheat endosperm Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal 2011 9(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal 2011 9(6)2207
Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249
Material Claimed effect EFSA Opinion Conditions of use EFSA Journal
Barley grain fibre Contributes to an increase in faecal bulk 20119(6)2249
Arabinoxylan (AX) produced from wheat endosperm (wheat grain fibre mainly soluble) Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal May be used only for food which contains at least 8 g AX- rich fibre produced from wheat endosperm (ge 60 AX) per 100 g of available carbohydrates in a quantified portion as part of the meal 20119(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal The claim may be used only for food which contains ge 4 g of beta-glucans from oats or barley for each 30 g of available carbohydrates in a quantified portion as part of the meal In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained by consuming the beta-glucans from oats or barley as part of the meal 20119(6)2207
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels The claim may be used only for food which contains ge 1 g of beta-glucans from oats oatbran barley barley bran or from mixtures of these sources per quantified portion In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained with a daily intake of 3g 2009 7(9)1254 20119(6)2207
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal The claim may be used only for food in which digestible starch has been replaced by resistant starch so that the final content of resistant starch is at least 14 of total starch 20119(4)2024
Whole grain(WG) WG flour WG foods Diets rich in WG gut healthbowel function weight control blood glucoseinsulin levels the food constituent whole grain which is the subject of this opinion is not sufficiently characterised in relation to the 20108(10)176
Page 9: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

β (1-31-4)glucans

Content 3-12

β(1-4)

β(1-3)

Content 4-6

Arabinoxylans

Dietary (Mostly Soluble) fiber

MS Izydorczyk Canada Grain Commi

II Simposio de Fisiologiacutea y Mejora de Cereales

Material Claimed effect EFSA Journal

Beta-glucans Contribute to the maintenance of normal blood cholesterol levels2009 7(9)1254 2011 9(6)2207

Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo

Wide range of claims 2010 8(10)1735

Whole grain(WG) WG flour WG foods Diets rich in WG

Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176

Resistant starchReplacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal

2011 9(4)2024

Arabinoxylan (AX) produced from wheat endosperm

Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal

2011 9(6)2205

Beta-glucans from oats and barley

Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal

2011 9(6)2207

Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249

httpshealthgrainorg

Barley-related Health Claim

Hoja1

Hoja2

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley and Oat β-Glucan Health Claim

American Food and Drug Administration (FDA) 2006 European Food Safety Authority (EFSA) 2011Health Canada 2012

Health claim Dosis

Reduction of the postprandial glycemicresponse

4 g per 30 g of carbohidrates

Maintenance of normal levels of LDL cholesterol in blood

1 g meal 3 g day

Reduction of blood cholesterol

3 gday 1 g portion

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
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  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Material Claimed effect EFSA Journal
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels 2009 7(9)1254 2011 9(6)2207
Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo Wide range of claims 2010 8(10)1735
Whole grain(WG) WG flour WG foods Diets rich in WG Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal 2011 9(4)2024
Arabinoxylan (AX) produced from wheat endosperm Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal 2011 9(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal 2011 9(6)2207
Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249
Material Claimed effect EFSA Opinion Conditions of use EFSA Journal
Barley grain fibre Contributes to an increase in faecal bulk 20119(6)2249
Arabinoxylan (AX) produced from wheat endosperm (wheat grain fibre mainly soluble) Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal May be used only for food which contains at least 8 g AX- rich fibre produced from wheat endosperm (ge 60 AX) per 100 g of available carbohydrates in a quantified portion as part of the meal 20119(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal The claim may be used only for food which contains ge 4 g of beta-glucans from oats or barley for each 30 g of available carbohydrates in a quantified portion as part of the meal In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained by consuming the beta-glucans from oats or barley as part of the meal 20119(6)2207
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels The claim may be used only for food which contains ge 1 g of beta-glucans from oats oatbran barley barley bran or from mixtures of these sources per quantified portion In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained with a daily intake of 3g 2009 7(9)1254 20119(6)2207
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal The claim may be used only for food in which digestible starch has been replaced by resistant starch so that the final content of resistant starch is at least 14 of total starch 20119(4)2024
Whole grain(WG) WG flour WG foods Diets rich in WG gut healthbowel function weight control blood glucoseinsulin levels the food constituent whole grain which is the subject of this opinion is not sufficiently characterised in relation to the 20108(10)176
Page 10: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Material Claimed effect EFSA Journal

Beta-glucans Contribute to the maintenance of normal blood cholesterol levels2009 7(9)1254 2011 9(6)2207

Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo

Wide range of claims 2010 8(10)1735

Whole grain(WG) WG flour WG foods Diets rich in WG

Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176

Resistant starchReplacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal

2011 9(4)2024

Arabinoxylan (AX) produced from wheat endosperm

Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal

2011 9(6)2205

Beta-glucans from oats and barley

Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal

2011 9(6)2207

Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249

httpshealthgrainorg

Barley-related Health Claim

Hoja1

Hoja2

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley and Oat β-Glucan Health Claim

American Food and Drug Administration (FDA) 2006 European Food Safety Authority (EFSA) 2011Health Canada 2012

Health claim Dosis

Reduction of the postprandial glycemicresponse

4 g per 30 g of carbohidrates

Maintenance of normal levels of LDL cholesterol in blood

1 g meal 3 g day

Reduction of blood cholesterol

3 gday 1 g portion

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
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  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Material Claimed effect EFSA Journal
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels 2009 7(9)1254 2011 9(6)2207
Dietary fibre (DF) rich in DF and ldquosoluble fibrerdquo Wide range of claims 2010 8(10)1735
Whole grain(WG) WG flour WG foods Diets rich in WG Gut healthbowel function weight control blood glucoseinsulin levels 2010 8(10)176
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal 2011 9(4)2024
Arabinoxylan (AX) produced from wheat endosperm Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal 2011 9(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal 2011 9(6)2207
Barley grain fibre Contributes to an increase in faecal bulk 2011 9(6)2249
Material Claimed effect EFSA Opinion Conditions of use EFSA Journal
Barley grain fibre Contributes to an increase in faecal bulk 20119(6)2249
Arabinoxylan (AX) produced from wheat endosperm (wheat grain fibre mainly soluble) Consumption of AX as part of a meal contributes to a reduction of the blood glucose rise after that meal May be used only for food which contains at least 8 g AX- rich fibre produced from wheat endosperm (ge 60 AX) per 100 g of available carbohydrates in a quantified portion as part of the meal 20119(6)2205
Beta-glucans from oats and barley Consumption of beta- glucans from oats or barley as part of a meal contributes to the reduction of the blood glucose rise after that meal The claim may be used only for food which contains ge 4 g of beta-glucans from oats or barley for each 30 g of available carbohydrates in a quantified portion as part of the meal In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained by consuming the beta-glucans from oats or barley as part of the meal 20119(6)2207
Beta-glucans Contribute to the maintenance of normal blood cholesterol levels The claim may be used only for food which contains ge 1 g of beta-glucans from oats oatbran barley barley bran or from mixtures of these sources per quantified portion In order to bear the claim information shall be given to the consumer that the beneficial effect is obtained with a daily intake of 3g 2009 7(9)1254 20119(6)2207
Resistant starch Replacing digestible starches with resistant starch in a meal contributes to a reduction in the blood glucose rise after that meal The claim may be used only for food in which digestible starch has been replaced by resistant starch so that the final content of resistant starch is at least 14 of total starch 20119(4)2024
Whole grain(WG) WG flour WG foods Diets rich in WG gut healthbowel function weight control blood glucoseinsulin levels the food constituent whole grain which is the subject of this opinion is not sufficiently characterised in relation to the 20108(10)176
Page 11: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley and Oat β-Glucan Health Claim

American Food and Drug Administration (FDA) 2006 European Food Safety Authority (EFSA) 2011Health Canada 2012

Health claim Dosis

Reduction of the postprandial glycemicresponse

4 g per 30 g of carbohidrates

Maintenance of normal levels of LDL cholesterol in blood

1 g meal 3 g day

Reduction of blood cholesterol

3 gday 1 g portion

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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  • Phenolic Compounds
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 12: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Ratio of papers published in the Web of Sciencein 2009-2018 and 1989-1998

0 200 400 600 800 1000

BIOCHEM MOL BIOMICROBIOLOGY

PHARMACOLOGYIMMUNOLOGY

BIOTECHN APPL MICROBIOCHEMISTRY ORGANIC

CHEMISTRY MULTIDISCCHEMISTRY APPLIED

INFECTIOUS DISEASESFOOD SCI TECHN

NUTRITION DIETETICSPOLYMER SCI

0

50

100

150

200

250

300

Glucans Barley Wheat Photosynthesis

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 13: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

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ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 14: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Phenolic Compounds

bull Free Phenols

bull Anthocyaninbull Bound Phenols

FlavonolsCatequinesProdelphinidin B3Procyanidin B3

Aacutecidos fenoacutelicos

Aacutecidos fenoacutelicosCoumaricCaffeicFeruacutelico

Barley Content 1000-4000 microgg

10 - 500 microgg

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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  • Phenolic Compounds
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 15: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Vitamin E (α β γ δ) Tocopherols amp (α β γ δ) Tocotrienols

Fuente M Martiacutenez (2016) TFM UdL

(microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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  • Phenolic Compounds
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  • Nuacutemero de diapositiva 21
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  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 16: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Resistant starch High amylose barley

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

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  • Phenolic Compounds
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 17: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Barley grown in Spain has high β-glucan content

(and β-glucanase activity)

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

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ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
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  • Phenolic Compounds
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 18: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Objectives

Assess the spatial variability in bioactive composition ofsequential fractions of two hull-less two-rowed waxy andnon-waxy food barley genotypes

Evaluate the potential interest of fractions derived from thebarley pearl to be used as ingredients for functional foods

Briefly present the food barley breeding program jointlycarried out by three Spanish institutions the James HuttonInstitute of Scotland and the Oregon State Barley Project

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 19: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Annapurnareg 2r hull-less waxy yellow grain 9 βglucan Hindukush 2r hull-less non-waxy purple grain 8 βglucan

Material and Methods

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 20: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Enriched fractions in bioactive compounds

Sequential pearling High in bioactive compounds High in β-glucans

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 21: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

β-glucans ( ww)

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

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hush

who

leHi

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heat

flou

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hole

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uts

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lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 22: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Arabinoxylans ( ww) Anthocyanins (microgg)

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 23: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Bound amp Free Phenols (microgg)

Bound amp Free ORAC (micromol Trolox100g)

Tocols (microgg)

Color ()

II Simposio de Fisiologiacutea y Mejora de Cereales

0

5000

10000

15000

20000

25000

Anna

purn

aHi

nduk

hush

who

leHi

nduk

hush

(0-2

0)

Refin

ed w

heat

flou

rW

hole

whe

atPu

rple

whe

atYe

llow

Whe

atRe

d W

heat

Oat

Whi

te so

rghu

mW

hite

rice

Who

le ri

ceBl

ack

rice

Blac

k qu

inoa

Yello

w p

eas

Gree

n pe

asLe

ntils

Com

mon

bea

nsBl

ack

Soyb

eans

Yello

w S

oybe

ans

Chick

pea

Alm

onds

Pist

achi

ous

Peca

nsHa

zel n

uts

Grap

e R

ed G

lobe

Grap

e B

lack

See

dlee

sBl

uebe

rry

Blu

egol

dBl

ackb

erry

Rasp

berr

yCh

erry

Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

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  • Nuacutemero de diapositiva 42
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 24: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

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Total Phenols (microgg)

05000

1000015000200002500030000350004000045000

ORAC (micromoles Trolox100g)

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 25: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Potentially functional biscuits (AACC Method 10-5005) by replacing refined wheatwith β-glucan extract whole barley flour or antioxidant enriched fractions

Biscuit CodeRefined

wheat flourWhole

wheat flourβminusglucan

extractWhole

barley flourBarley external (0-15) fraction

Wheat RW 100Whole wheat WW 100Wheat and βminusglucan extract βW 87 13Whole barley ( Annapurna ) BA 100Whole barley ( Hindukush ) BH 100Wheat and Annapurna 30BA 70 30Wheat and Hindukush 30BH 70 30Wheat and Annapurna (external fraction) 30eA 70 30Wheat and Hindukush (external fraction) 30eH 70 30

Functional Foods

filtrada

Horneado

Hoja1

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Biscuit Code Refined wheat flour Whole wheat flour b-glucan extract Whole barley flour Barley external (0-15) fraction
Wheat RW 100
Whole wheat WW 100
Wheat and b-glucan extract bW 87 13
Whole barley ( Annapurna ) BA 100
Whole barley ( Hindukush ) BH 100
Wheat and Annapurna 30BA 70 30
Wheat and Hindukush 30BH 70 30
Wheat and Annapurna (external fraction) 30eA 70 30
Wheat and Hindukush (external fraction) 30eH 70 30
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext A_A II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext A_A III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext H _A I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext H _A II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext H _A III 810 230466 360535 591000 13258
C A Galleta C Galleta C 100C A_C I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C A_C II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C A_C III 903 588 156401 501
C H Galleta C Galleta C 100C H _C I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C H _C II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C H _C III 192637 547996 740632
TC A Galleta E Galleta E 70T30C A_E I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C A_E II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C A_E III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C H _E I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C H _E II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C H _E III 465 102619 174611 277230 2252
T TR Galleta T Galleta T 100T TR_T I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T TR_T II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T TR_T III 273 33700 44156 77856 224
T TI Galleta TI Galleta I 100T TI_I I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T TI_I II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T TI_I III 917 42770 57837 100607 019
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 236 30492 30222 60715 094
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C III 635 14334
TC A Harina E Harina E 70T30C A_E I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Page 26: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Bioactive compounds in Biscuits

65

18

30BH

BHBA

30eH30eA

WW

RW

βW

β Glucans

Arabinoxylans

Anthocyanins

Free Phenols

Bound Phenols

Free amp Bound Phenols ORAC

30BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Page 27: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effectsβ-Glucans

AnthocyaninsFlourBiscuit (corrected)

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Page 28: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Baking effects

FlourBiscuit (corrected)

Arabinoxylans

Amylose

Total Phenols

Total ORAC

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Page 29: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsβ-Glucans ( ww)

Number of biscuits needed to fulfill the Health ClaimRW WW βW 30BA 30BH 30eA 30eH BA BH

3 gday 105 37 24 13 13 20 26 32 35

RW WW βgW BA BH 30BA 30BH 30eA 30eH

Hoja1

Hoja2

filtrada

Horneado

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
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Especies Genotipo Code_1 Material Code_2 Tipo Code_3 Repeticioacuten Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu bgW III 242 2870 4972 7842 032
T TR Harina T Harina T 100T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T rW III 3370 4210 7580
C A Harina C Harina C 100C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C wBH III 635 14334
TC A Harina A Harina A 70T30Cext WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext WeH III 841 17061 27677 44737 26625
T TI Harina TI Harina I 100T wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T wW III 779 5785 6839 12624
TC A Harina E Harina E 70T30C WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C WwH III 388 3439 13950 17389 7206
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW I 6749 1297 1110 353 28934 28267 57200 122 264291 329253 593543
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW II 6472 1341 1283 291 29489 28816 58305 095 201839 288965 490804
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu bgW III 236 30492 30222 60715 094
T TR Galleta T Galleta T 100T rW I 8166 1518 027 295 33036 44269 77306 137699 161031 298730
T TR Galleta T Galleta T 100T rW II 7298 1454 028 295 35389 47154 82543 195 188631 198449 387080
T TR Galleta T Galleta T 100T rW III 273 33700 44156 77856 224
C A Galleta C Galleta C 100C wBA I 5151 446 845 620 199481 164877 364358 589861 402492 992353
C A Galleta C Galleta C 100C wBA II 5191 488 895 581 201538 169161 370699 494 561248 339089 900337
C A Galleta C Galleta C 100C wBA III 903 588 156401 501
C H Galleta C Galleta C 100C wBH I 5614 715 835 628 189319 500139 689458 6354 608530 528552 1137082
C H Galleta C Galleta C 100C wBH II 5036 728 781 610 194854 522015 716869 6858 644877 576640 1221517
C H Galleta C Galleta C 100C wBH III 192637 547996 740632
TC A Galleta A Galleta A 70T30Cext WeA I 5549 615 144 804 102581 323814 426395 607 737276 594814 1332089
TC A Galleta A Galleta A 70T30Cext WeA II 5808 732 136 782 101841 289829 391670 657750 557241 1214991
TC A Galleta A Galleta A 70T30Cext WeA III 790 102619 319032 421651 624
TC H Galleta A Galleta A 70T30Cext WeH I 3541 865 118 903 13281 1053038 781818 1834856
TC H Galleta A Galleta A 70T30Cext WeH II 3717 1101 101 791 180982 359133 540115 13123 924431 754007 1678438
TC H Galleta A Galleta A 70T30Cext WeH III 810 230466 360535 591000 13258
T TI Galleta TI Galleta I 100T wW I 6122 1221 076 814 45294 57116 102410 019 323008 332746 655754
T TI Galleta TI Galleta I 100T wW II 6013 1273 957 42467 60228 102694 015 327102 331792 658894
T TI Galleta TI Galleta I 100T wW III 917 42770 57837 100607 019
TC A Galleta E Galleta E 70T30C WwA I 6160 1497 236 564 68558 124706 193264 771 288187 199479 487666
TC A Galleta E Galleta E 70T30C WwA II 6778 1356 211 491 73966 132239 206205 718 250544 243940 494484
TC A Galleta E Galleta E 70T30C WwA III 404 72277 132505 204782 685
TC H Galleta E Galleta E 70T30C WwH I 6511 1754 209 431 27097 162315 189412 2215 281805 393708 675513
TC H Galleta E Galleta E 70T30C WwH II 6591 1881 214 397 68767 174288 243055 2437 257909 346870 604779
TC H Galleta E Galleta E 70T30C WwH III 465 102619 174611 277230 2252
C A Fraccion Bran Fraccio bran 015 A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Especies Genotipo Code_1 Flour vs Biscuit Code_2 Tipo Code_3 Material Repeticioacuten Grasa Proteina Ceniza Almidoacuten Amilosa Be_glu AX FF FB F_TOTAL AN AOX_FF AOX_BB AOX_TOTAL
TC A Galleta A Galleta A 70T30Cext A_A WeA I 10281 7941 1820 2924 324 076 424 5406 17065 22471 320 3885 3135 7020
TC A Galleta A Galleta A 70T30Cext A_A WeA II 10652 7991 1782 3061 386 072 412 5367 15274 20641 3466 2937 6403
TC A Galleta A Galleta A 70T30Cext A_A WeA III 10704 7884 1798 416 5408 16813 22221 329
TC H Galleta A Galleta A 70T30Cext H _A WeH I 11774 8238 2427 1866 456 062 476 6999 5550 4120 9670
TC H Galleta A Galleta A 70T30Cext H _A WeH II 12271 8389 2571 1959 580 053 417 9538 18926 28464 6916 4872 3974 8845
TC H Galleta A Galleta A 70T30Cext H _A WeH III 11897 8458 2504 427 12146 19000 31146 6987
C A Galleta C Galleta C 100C A_C wBA I 9637 7985 1604 2715 235 445 327 10513 8689 19202 3109 2121 5230
C A Galleta C Galleta C 100C A_C wBA II 9704 8154 1600 2736 257 471 306 10621 8915 19536 260 2958 1787 4745
C A Galleta C Galleta C 100C A_C wBA III 10133 8104 1612 476 310 8242 264
C H Galleta C Galleta C 100C H _C wBH I 9986 8603 2023 2959 377 440 331 9977 26357 36334 3348 3207 2785 5992
C H Galleta C Galleta C 100C H _C wBH II 10204 8838 2057 2654 384 411 322 10269 27510 37779 3614 3399 3039 6437
C H Galleta C Galleta C 100C H _C wBH III 10071 8665 2103 10152 28879 39031
TC A Galleta E Galleta E 70T30C A_E WwA I 9595 7498 1325 3246 789 124 297 3613 6572 10185 407 1519 1051 2570
TC A Galleta E Galleta E 70T30C A_E WwA II 9400 7354 1408 3572 714 111 259 3898 6969 10867 379 1320 1286 2606
TC A Galleta E Galleta E 70T30C A_E WwA III 9702 7410 1417 213 3809 6983 10792 361
TC H Galleta E Galleta E 70T30C H _E WwH I 9760 7584 1428 3431 924 110 227 1428 8554 9982 1167 1485 2075 3560
TC H Galleta E Galleta E 70T30C H _E WwH II 9726 7789 1439 3473 992 113 209 3624 9185 12809 1284 1359 1828 3187
TC H Galleta E Galleta E 70T30C H _E WwH III 10042 7578 1450 245 5408 9202 14610 1187
T TR Galleta T Galleta T 100T TR_T rW I 9093 5402 1203 4303 800 014 155 1741 2333 4074 726 849 1574
T TR Galleta T Galleta T 100T TR_T rW II 9058 5329 1225 3846 766 014 155 1865 2485 4350 103 994 1046 2040
T TR Galleta T Galleta T 100T TR_T rW III 9142 5259 1222 144 1776 2327 4103 118
T TI Galleta TI Galleta I 100T TI_I wW I 10827 7280 1605 3226 643 040 429 2387 3010 5397 010 1702 1754 3456
T TI Galleta TI Galleta I 100T TI_I wW II 10572 7280 1603 3169 671 504 2238 3174 5412 008 1724 1749 3472
T TI Galleta TI Galleta I 100T TI_I wW III 11513 7280 1645 483 2254 3048 5302 010
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW I 8572 5123 1345 3557 683 585 186 1525 1490 3014 064 1393 1735 3128
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW II 9494 5263 1350 3411 707 676 153 1554 1519 3073 050 1064 1523 2587
T Tb-glu Galleta Tb-glu Galleta Tb 87T13b-glu Tb-glu_ Tb bgW III 9784 5132 1330 124 1607 1593 3200 049
TC A Harina A Harina A 70T30Cext A_A WeA I 5546 1247 148 798 15186 24295 39481 7432 3151 10583
TC A Harina A Harina A 70T30Cext A_A WeA II 5153 1249 077 764 14826 26584 41410 451 7495 3743 11238
TC A Harina A Harina A 70T30Cext A_A WeA III 770 15389 26482 41871 452
TC H Harina A Harina A 70T30Cext H _A WeH I 4943 1507 158 815 28330 7065 4156 11221
TC H Harina A Harina A 70T30Cext H _A WeH II 4934 1547 061 824 17282 27564 44845 27073 7040 4437 11478
TC H Harina A Harina A 70T30Cext H _A WeH III 841 17061 27677 44737 26625
C A Harina C Harina C 100C A_C wBA I 4494 544 810 786 20436 17574 38010 485 4537 2734 7271
C A Harina C Harina C 100C A_C wBA II 5488 693 796 685 21637 18265 39902 452 4657 2868 7526
C A Harina C Harina C 100C A_C wBA III 734 20510 17630 38140 439
C H Harina C Harina C 100C H _C wBH I 5099 2020 831 618 12957 39916 52873 15788 6204 6968 13172
C H Harina C Harina C 100C H _C wBH II 5230 2000 821 625 12233 43030 55262 15602 6174 6505 12680
C H Harina C Harina C 100C H _C wBH III 635 14334
TC A Harina E Harina E 70T30C A_E WwA I 6622 1750 273 385 8595 10152 18747 1985 1429 3414
TC A Harina E Harina E 70T30C A_E WwA II 6133 1707 275 354 8180 10571 18751 495 1840 1753 3593
TC A Harina E Harina E 70T30C A_E WwA III 390 8400 10877 19277 522
TC H Harina E Harina E 70T30C H _E WwH I 6411 2175 267 352 3216 14697 17913 7212 2603 2445 5048
TC H Harina E Harina E 70T30C H _E WwH II 6329 2241 255 409 3624 14495 18119 7391 2337 2194 4532
TC H Harina E Harina E 70T30C H _E WwH III 388 3439 13950 17389 7206
T TR Harina T Harina T 100T TR_T rW I 7347 2396 019 288 2981 054 1248 1084 2332
T TR Harina T Harina T 100T TR_T rW II 6899 2125 026 340 3571 3810 7381 084 1045 1032 2077
T TR Harina T Harina T 100T TR_T rW III 3370 4210 7580
T TI Harina TI Harina I 100T TI_I wW I 5397 1285 082 735 145 1666 2931 4598
T TI Harina TI Harina I 100T TI_I wW II 5811 1190 084 589 5827 7094 12921 148 1442 2855 4297
T TI Harina TI Harina I 100T TI_I wW III 779 5785 6839 12624
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW I 6181 1524 144 294 2923 4630 7552 045 1318 1764 3081
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW II 6334 1554 131 266 2945 5094 8040 050 1783 1512 3295
T Tb-glu Harina Tb-glu Harina Tb 87T13b-glu Tb-glu_ Tb bgW III 242 2870 4972 7842 032
C A Fraccion Bran Fraccio bran 015 A_bran A_bran I 1320 1806 27 2335 59445 61360 120805 1084 25952 11826 37778
C A Fraccion Bran Fraccio bran 015 A_bran A_bran II 1353 1982 51 1816 46955 48421 95376 793 14680 11348 26027
C A Fraccion Bran Fraccio bran 015 A_bran A_bran III 79 1454 26767 30599 57366 688 11376 6066 17442
C H Fraccion Bran Fraccio bran 015 H_bran A_bran I 609 2409 07 2504 31638 81690 113328 27426 12636 15403 28039
C H Fraccion Bran Fraccio bran 015 H_bran A_bran II 593 2463 09 2215 69282 88724 158006 68591 22715 17826 40541
C H Fraccion Bran Fraccio bran 015 H_bran A_bran III 22 1949 60961 95330 156291 68204 25322 18835 44158
C H Fraccion Bran Fraccio bran 015 H_bran A_bran IV 51 1683 46877 81478 128355 36083 17873 15994 33867
Promedio de AOX_TOTAL Etiquetas de columna
Etiquetas de fila Fraccio Galleta Harina (en blanco) Total general
A_bran 325503626688138 325503626688138
bgW 28572546564238 31879745637772 30226146101005
rW 18071092084001 22044938234148 20058015159075
wBA 49872372474179 73983077675575 61927725074877
wBH 62149078679948 129258024020206 95703551350077
WeA 6711556465968 109104268570488 88109916615084
WeH 92575304016251 113495478521452 103035391268851
wW 34640982443069 44475526067722 39558254255395
WwA 25879648117575 35035527803258 30457587960416
WwH 33735679973164 47898976383694 40817328178429
(en blanco)
Total general 325503626688138 45845807668012 67463951434924 10042095466674
Promedio de Be_glu
Etiquetas de fila Galleta
bgW 63041648451 24 06
rW 01429296108 1049 262
wBA 46434010538 32 08
wBH 42585558849 35 09
WeA 07387220096 203 51
WeH 05765238587 260 65
wW 04007424635 374 94
WwA 11764543043 128 32
WwH 1116835442 134 34
(en blanco)
Total general 2386628752
RW WW bW 30BA 30BH 30eA 30eH BA BH
3 gday 105 37 24 13 13 20 26 32 35
075 gserving 26 9 06 08 09 32 34 51 65
Page 30: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsDietary Fiber β-Glucans + Arabinoxylans ( ww)

6

3

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Page 31: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Functional properties of biscuitsTotal ORAC (micromol Trolox100g)

Grapes LentilsPistachios

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Page 32: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical properties

49

30

30BH

BH

30BA

30eH

30eA

WWRW

βW

Color_b

Volume

WT

Diet Fiber

Density

Color_a

Color_L

Strength

BA

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Page 33: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Physical propertiesof biscuits

Density

Strength (N)

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Page 34: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Best biscuits

β- glucan RW WW βW 30BA 30BH 30eA 30eH BA BH

Total Dietary Fiber RW WW βW 30BA 30BH 30eA 30eH BA BH

Antioxidant activity RW WW βW 30BA 30BH 30eA 30eH BA BH

Physical Properties RW WW βW 30BA 30BH 30eA 30eH BA BH

Do they taste good

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Page 35: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Food Barley Breeding

1 High βminusglucan adapted to Spain2 Hull-less high βminusglucan with enhanced antioxidant activity3 Hull-less high βminusglucan with antioxidant activity high amylose

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Page 36: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

1 Phase I High βminusglucan adapted to Spainbull Kamalamaireg Two-row hulled high β-glucan waxy high yield bull Annapurnareg Two-row hull-less high β-glucan waxy bull Rajapanireg Six-row hull-less high β-glucan non-waxy

2 Phase II Hull-less high β-glucan with enhanced antioxidant activitybull 400 DHL under selection at Bell-Lloch Dundee Corvallis

3 Phase III Hull-less high βminusglucan with enhanced antioxidant activity high amylosebull First DHL are being produced crossing advanced lines from Phase II with a

High Amylose Canadian donor

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Page 37: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Page 38: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

The bioactive compounds are spatially distributeddifferentially in the barley grain The pearling allowsobtaining fractions differentially rich in bioactivecompounds

Fractions derived from the barley pearl can be used asingredients for functional foods such as biscuitsbakery products or pasta rich in dietary fiberphenolic compounds or antioxidants

Two rowed hull-less high in dietary fiber (β-glucanarabinoxylan) phenols (including anthocyanintocopherols tocotrienols) amylose content arebeing characterized to identify a next generation offood barley genotypes

However regardless of their healthy value food barleywill only reach the market if attractive and tasty foodproducts are developed which remains a challenge

Conclusions

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Page 39: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Is there a market for Food Barley in Spain

Dos estudiantes de la UPNA idean un cafeacute soluble enriquecido con una fibra vegetal que protege la salud cardiovascularldquo 1erPremios Pascual Start up

Lordi a taula (Barley to the table) Premio UB - Ferran Adriagrave amb Gallina Blanca

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Page 40: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Why did Roman Gladiators eat barley

We can only guess but on top of its potential benefits as food keepingin mind their main activity it may not have not been such a bad option

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
  • Nuacutemero de diapositiva 23
  • Nuacutemero de diapositiva 24
  • Nuacutemero de diapositiva 25
  • Nuacutemero de diapositiva 26
  • Nuacutemero de diapositiva 27
  • Nuacutemero de diapositiva 28
  • Nuacutemero de diapositiva 29
  • Nuacutemero de diapositiva 30
  • Nuacutemero de diapositiva 31
  • Nuacutemero de diapositiva 32
  • Nuacutemero de diapositiva 33
  • Nuacutemero de diapositiva 34
  • Nuacutemero de diapositiva 35
  • Nuacutemero de diapositiva 36
  • Nuacutemero de diapositiva 37
  • Nuacutemero de diapositiva 38
  • Nuacutemero de diapositiva 39
  • Nuacutemero de diapositiva 40
  • Nuacutemero de diapositiva 41
  • Nuacutemero de diapositiva 42
Page 41: Why did Roman Gladiators eat barley? Barley, a healthy grainsefimec.csic.es/wp-content/uploads/2019/03/Ignacio-Romagosa.pdfII Simposio de Fisiología y Mejora de Cereales Gaius Plinius

II Simposio de Fisiologiacutea y Mejora de Cereales

Mariona Martiacutenez-Subiragrave Luis Cistueacute Bill Thomas Pat Hayes Paco Batlle Mari Paz Romero Marian Moralejo

Agrotecnio Universitat de Lleida Lleida SpainEstacioacuten Experimental de Aula Dei ndash CSIC Zaragoza Spain

Semillas Batlle Bell-Lloch drsquoUrgell Lleida Spain

The James Hutton Institute Dundee Scotland United KingdomOregon State University Corvallis Oregon USA

AGL2015-69435-C3-R

II Simposio de Fisiologiacutea y Mejora de Cereales

Thanks for your attention

  • Nuacutemero de diapositiva 1
  • Nuacutemero de diapositiva 2
  • Nuacutemero de diapositiva 3
  • Nuacutemero de diapositiva 4
  • Nuacutemero de diapositiva 5
  • Nuacutemero de diapositiva 6
  • Nuacutemero de diapositiva 7
  • Nuacutemero de diapositiva 8
  • Nuacutemero de diapositiva 9
  • Nuacutemero de diapositiva 10
  • Nuacutemero de diapositiva 11
  • Nuacutemero de diapositiva 12
  • Nuacutemero de diapositiva 13
  • Phenolic Compounds
  • Nuacutemero de diapositiva 15
  • Nuacutemero de diapositiva 16
  • Nuacutemero de diapositiva 17
  • Nuacutemero de diapositiva 18
  • Nuacutemero de diapositiva 19
  • Nuacutemero de diapositiva 20
  • Nuacutemero de diapositiva 21
  • Nuacutemero de diapositiva 22
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II Simposio de Fisiologiacutea y Mejora de Cereales

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