Dr. Alvin Yeh Department of Biomedical Engineering Blood
Harvest: Laboratory Creation of Blood Vessels Josh Bergerson
Normangee High School
Slide 2
Virtual Model of Angiogenesis Important Process In: normal
tissue repairmultiple diseasesengineered tissue.
Slide 3
In Vitro Angiogenesis Model Can study the effects of Varying
collagen stiffness Varying collagen thickness Varying collagen
concentration Endothelial cell monolayer Collagen gel preparation
Serumfree medium
Slide 4
The Results
Slide 5
Electromagnetic spectrum Properties of waves Experimental
design Optics & imaging PolymersFluorescenceMechanics PC data
collection Elements of Research Applied in the Classroom
Slide 6
Relevance to Standards
Slide 7
Learning Objectives
Slide 8
Materials Borax Cardboard Silicon mold Computer paper Corn meal
Sugar Salt Baking soda Clear glue Water Gelatin Fishing line Water
Laser pointers(r/g/b) Meter stick Weight set Spring scale Ruler
Beakers Craft sticks Binder clips Calculators Force sensor (opt)
Light sensor (opt) Go-link(opt) scissors packing tape Food coloring
(opt)
Slide 9
Classroom Lesson Day 1
Slide 10
Classroom Lesson Day 2 Matrix Constraints Must be smaller than
5cm x 5cm x 2cm Must contain fishing line Must be transparent
Matrix Preparation Make mold (silicon/Cardboard) Mix polymer matrix
Add additives (opt) Let gels set overnight Corn meal Salt Sugar
Baking Powder
Slide 11
Classroom Lesson Day 3 Mechanical Testing Force sensor to
measure x/y strength Mass set to test z strength Imaging the Sample
Image with R/G/B Record diameter of sample Light sensor to gauge
transparency of gel Testing the Matrix
Slide 12
Classroom Lesson Day 4 Image Analysis Use diffraction pattern
to find diameter of sample Compare results of intensity with R/G/B
Concluding Presentation Present matrix & data to class Suggest
improvements they would make Compare class data to find most
efficient model
Slide 13
Sample Pre- and Post- Test Questions Which of the following is
the result of excessive angiogenesis? A stroke Cancer Tissue growth
Tissue Repair Which color of visible light has the most energy? Red
Green Blue Ultraviolet (True / False) Polymers with different
chemical compositions may have different physical properties. (True
/ False) A polymers color is an example of an extensive
property.
Slide 14
Sources From the Web uwbwritingcenter.wordpress.com
en.wikipedia.org stacymakescents.com unschoolme.blogspot.com
http://stemak.org/sites/default/files/Measuring%20the%2
0diameter%20of%20a%20hair%20using%20a%20laser.pdf
http://stemak.org/sites/default/files/Measuring%20the%2
0diameter%20of%20a%20hair%20using%20a%20laser.pdf
http://www.vernier.com/products/sensors/force- sensors/dfs-bta/
biology.clc.uc.edu
Slide 15
Acknowledgements TAMU E3 RET Program National Science
Foundation Nuclear Power Institute Dr. Alvin Yeh Research Group:
Tissue Microscopy Lab Dr. Arne Lekven Lab Group