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PENGENALAN KULTUR MEDIA BAKTERI

DI EDIT OLEH PROF. DR.DRH .PRATIWI TS,MS

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KULTUR MEDIA BACTERI UNTUK APA ?

04/20/23 KULTUR MEDIA BAKTERI-PTS PET 2

APA YANG DIPERLUKAN?


KEBUTUHAN OKSIGEN


TUJUAN MELAKUKAN PENANAMAN


ISOLASI KULTUR MURNI


TIPE MEDIA TANAM





MEDIA CAIR










Kuliah berikut





fuchsine

Gram Staining

Technique



















• Goal: To chemically (or physically) suppress unwanted microbes and encourage desired microbes.

Selective Media

Figure 6.9b, c

Mannitol salt agar : selective for halophiles with 7% salt (osmotic challenge) and differential for mannitol fermenters: good for skin bacterial cultures.

EMB Agar: kills gram positives with eosin and methylene blue, selective for gram negatives. Differential for lactose fermenters. Good for growing enterics.

McConkey Agar: supresses gram positives with crystal violet and bile salts; also differential for

MSA

EMB

MA


• Distinguish between different species based on a metabolic ability.

Differential Media

Figure 6.9a

Blood agar(sheep’s blood) reveals if hemolytic

Mannitol salt agar contains the pH sensitive dye phenol red (yellow when acidic)

Se Sa


Direct Measurements of Microbial Growth

Figure 6.1904/20/23 45KULTUR MEDIA BAKTERI-PTS PET

Estimating Bacterial Numbers by Indirect methods

• Direct Measures– Plate counts of viable bacterial forming colonies– Counting low viable bacterial numbers by filtration– Counting viable bacteria with Most Probable Number– Counting bacteria per ml in direct microscopy

• Indirect Measures– Turbidity/Absorbance with a spectrophotometer– Metabolic activity tracking conversion of colored

molecules/enyzme assay– Dry weight by weighing a set volume and knowing weight

of one cell






KULTUR MEDIA BAKTERI-PTS PET

Phases of Growth• Lag

– Adapt to nutrients• Log

– Active growth• Stationary

– Death = Growth rate• Death

– Nutrients consumed– pH too low (why?)

• Optimize curves in production

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KULTUR MEDIA BAKTERI-PTS PET

Log Growth

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Figure 6.14

Growth Curve for Bacteria (Logarithmic Plot)


Estimating Bacterial Numbers by Indirect methods

• Direct Measures– Plate counts of viable bacterial forming colonies– Counting low viable bacterial numbers by filtration– Counting viable bacteria with Most Probable Number– Counting bacteria per ml in direct microscopy

• Indirect Measures– Turbidity/Absorbance with a spectrophotometer– Metabolic activity tracking conversion of colored

molecules– Dry weight by weighing a set volume and knowing weight

of one cell


