PENGENALAN KULTUR MEDIA BAKTERI
DI EDIT OLEH PROF. DR.DRH .PRATIWI TS,MS
04/20/23 1KULTUR MEDIA BAKTERI-PTS PET
KULTUR MEDIA BACTERI UNTUK APA ?
04/20/23 KULTUR MEDIA BAKTERI-PTS PET 2
APA YANG DIPERLUKAN?
04/20/23 KULTUR MEDIA BAKTERI-PTS PET 3
KEBUTUHAN OKSIGEN
04/20/23 4KULTUR MEDIA BAKTERI-PTS PET
TUJUAN MELAKUKAN PENANAMAN
04/20/23 KULTUR MEDIA BAKTERI-PTS PET 5
ISOLASI KULTUR MURNI
04/20/23 6KULTUR MEDIA BAKTERI-PTS PET
TIPE MEDIA TANAM
04/20/23 7KULTUR MEDIA BAKTERI-PTS PET
04/20/23 8KULTUR MEDIA BAKTERI-PTS PET
04/20/23 9KULTUR MEDIA BAKTERI-PTS PET
04/20/23 10KULTUR MEDIA BAKTERI-PTS PET
MEDIA CAIR
04/20/23 11KULTUR MEDIA BAKTERI-PTS PET
04/20/23 12KULTUR MEDIA BAKTERI-PTS PET
04/20/23 13KULTUR MEDIA BAKTERI-PTS PET
04/20/23 14KULTUR MEDIA BAKTERI-PTS PET
04/20/23 15KULTUR MEDIA BAKTERI-PTS PET
04/20/23 16KULTUR MEDIA BAKTERI-PTS PET
04/20/23 17KULTUR MEDIA BAKTERI-PTS PET
04/20/23 18KULTUR MEDIA BAKTERI-PTS PET
04/20/23 19KULTUR MEDIA BAKTERI-PTS PET
Kuliah berikut
04/20/23 KULTUR MEDIA BAKTERI-PTS PET 20
04/20/23 21KULTUR MEDIA BAKTERI-PTS PET
04/20/23 22KULTUR MEDIA BAKTERI-PTS PET
04/20/23 23KULTUR MEDIA BAKTERI-PTS PET
fuchsine
Gram Staining
Technique
04/20/23 25KULTUR MEDIA BAKTERI-PTS PET
04/20/23 26KULTUR MEDIA BAKTERI-PTS PET
04/20/23 27KULTUR MEDIA BAKTERI-PTS PET
04/20/23 28KULTUR MEDIA BAKTERI-PTS PET
04/20/23 29KULTUR MEDIA BAKTERI-PTS PET
04/20/23 30KULTUR MEDIA BAKTERI-PTS PET
04/20/23 31KULTUR MEDIA BAKTERI-PTS PET
04/20/23 32KULTUR MEDIA BAKTERI-PTS PET
04/20/23 33KULTUR MEDIA BAKTERI-PTS PET
04/20/23 34KULTUR MEDIA BAKTERI-PTS PET
04/20/23 35KULTUR MEDIA BAKTERI-PTS PET
04/20/23 36KULTUR MEDIA BAKTERI-PTS PET
04/20/23 37KULTUR MEDIA BAKTERI-PTS PET
04/20/23 38KULTUR MEDIA BAKTERI-PTS PET
04/20/23 39KULTUR MEDIA BAKTERI-PTS PET
04/20/23 40KULTUR MEDIA BAKTERI-PTS PET
04/20/23 41KULTUR MEDIA BAKTERI-PTS PET
04/20/23 42KULTUR MEDIA BAKTERI-PTS PET
• Goal: To chemically (or physically) suppress unwanted microbes and encourage desired microbes.
Selective Media
Figure 6.9b, c
Mannitol salt agar : selective for halophiles with 7% salt (osmotic challenge) and differential for mannitol fermenters: good for skin bacterial cultures.
EMB Agar: kills gram positives with eosin and methylene blue, selective for gram negatives. Differential for lactose fermenters. Good for growing enterics.
McConkey Agar: supresses gram positives with crystal violet and bile salts; also differential for
MSA
EMB
MA
04/20/23 43KULTUR MEDIA BAKTERI-PTS PET
• Distinguish between different species based on a metabolic ability.
Differential Media
Figure 6.9a
Blood agar(sheep’s blood) reveals if hemolytic
Mannitol salt agar contains the pH sensitive dye phenol red (yellow when acidic)
Se Sa
04/20/23 44KULTUR MEDIA BAKTERI-PTS PET
Direct Measurements of Microbial Growth
Figure 6.1904/20/23 45KULTUR MEDIA BAKTERI-PTS PET
Estimating Bacterial Numbers by Indirect methods
• Direct Measures– Plate counts of viable bacterial forming colonies– Counting low viable bacterial numbers by filtration– Counting viable bacteria with Most Probable Number– Counting bacteria per ml in direct microscopy
• Indirect Measures– Turbidity/Absorbance with a spectrophotometer– Metabolic activity tracking conversion of colored
molecules/enyzme assay– Dry weight by weighing a set volume and knowing weight
of one cell
04/20/23 46KULTUR MEDIA BAKTERI-PTS PET
04/20/23 47KULTUR MEDIA BAKTERI-PTS PET
04/20/23 KULTUR MEDIA BAKTERI-PTS PET 48
04/20/23 KULTUR MEDIA BAKTERI-PTS PET 49
04/20/23 KULTUR MEDIA BAKTERI-PTS PET 50
KULTUR MEDIA BAKTERI-PTS PET
Phases of Growth• Lag
– Adapt to nutrients• Log
– Active growth• Stationary
– Death = Growth rate• Death
– Nutrients consumed– pH too low (why?)
• Optimize curves in production
04/20/23 51
KULTUR MEDIA BAKTERI-PTS PET
Log Growth
04/20/23 52
KULTUR MEDIA BAKTERI-PTS PET04/20/23 53
Figure 6.14
Growth Curve for Bacteria (Logarithmic Plot)
04/20/23 54KULTUR MEDIA BAKTERI-PTS PET
Estimating Bacterial Numbers by Indirect methods
• Direct Measures– Plate counts of viable bacterial forming colonies– Counting low viable bacterial numbers by filtration– Counting viable bacteria with Most Probable Number– Counting bacteria per ml in direct microscopy
• Indirect Measures– Turbidity/Absorbance with a spectrophotometer– Metabolic activity tracking conversion of colored
molecules– Dry weight by weighing a set volume and knowing weight
of one cell
04/20/23 55KULTUR MEDIA BAKTERI-PTS PET
04/20/23 KULTUR MEDIA BAKTERI-PTS PET 56
04/20/23 KULTUR MEDIA BAKTERI-PTS PET 57