63rdAnnualWindRiverConferenceonProkaryoticBiology
Prokaryotic Biology
- The Key to Understanding The Complexity of Life -
June5-9,2019
DaoHouseEstesPark
ColoradoUSA
63rdAnnualWindRiverConference,June5-9,2019
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WELCOMETOTHE63rdANNUALWINDRIVERCONFERENCEONPROKARYOTICBIOLOGY2019
WindRiverExecutiveCommittee:• Dr.HelenWing,UniversityofNevada,LasVegas• Dr.ErinMurphy,OhioUniversity• Dr.EthanMann,SharkletTechnologies,Inc.• Dr.MarioPedraza-Reyes,UniversityofGuanajuato• Dr.EduardoRobleto,UniversityofNevada,LasVegas• Dr.CorrieDetweiler,UniversityofColoradoBoulder• Dr.R.Martin(Marty)Roop,EastCarolinaUniversity • Dr.DanielWozniak,OhioStateUniversity • Dr.MichaelSchurr,UniversityofColoradoAnschutzMedicalCampus • Dr.AmandaOglesby-Sherrouse,UniversityofMaryland
Scientificprogramchair:
• Dr.ClaytonCaswell,VirginiaTech Contributions from the following sponsors helpedmake this year’s conference possible and are gratefullyacknowledged:MonserateBiotechnologyGroupSharkletBiotechnologies,Inc.TheDaoHouseUNLV,SchoolofLifeSciencesSpecialacknowledgement:
• Special thanks are due to Rosy Moloche Garcia at UNLV for expert administrativeassistance.
• Specialthankstoalltraineeswhovolunteeredtheirtimetohelpwiththemeeting.• Special thankstoEduardoRobletoandHelenWingatUNLVfor invaluableacademicand
administrativesupport.• SpecialthankstoEduardoRobletoforgrantwritingactivities.
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THEWINDRIVERHALLOFFAME:PASTANDPRESENTPLENARYSPEAKERS2019 Dr.ChristineJacobs-Wagner YaleUniversity
Dr.ElizabethRucks UniversityofNebraskaMedicalCenterDr.ClayFuqua IndianaUniversityBloomingtonDr.ChristopherWaters MichiganStateUniversity
2018 Dr.DeniseM.Monack StanfordUniversity Dr.AnthonyMaurelli UniversityofFlorida,EmergingPathogensInstitute Dr.AmyVollmer SwarthmoreCollege Dr.AlfredoTorres UniversityofTexasMedicalBranchatGalveston2017 Dr.MelodyMills NIH,NIAID
Dr.CatherineDrennan MassachusettsInstituteofTechnologyDr.MatthewParsek UniversityofWashingtonDr.ForestRohwer SanDiegoStateUniversity
2016 Dr.GrahamWalker MassachusettsInstituteofTechnology Dr.EvgenyNudler NYUSchoolofMedicine Dr.VictorNizet UniversityofCalifornia,SanDiego Dr.PeterSetlow UniversityofConnecticutHealthSciencesCenter2015 Dr.VictorTorres NYUSchoolofMedicine Dr.NancyFreitag UniversityofIllinois-Chicago Dr.DavidWeiss EmoryUniversity Dr.FranzNarberhaus RuhrUniversity2014 Dr.MichaelGilmore HarvardMedicalSchool
Dr.GiselaStorz NationalInstitutesofHealth Dr.FerranGarcia-Pichel ArizonaStateUniversity Dr.BarbaraFunnell UniversityofToronto2013 Dr.NinaSalama FredHutchinsonCancerInstitute Dr.ShelleyPayne UniversityofTexasatAustin Dr.ReneeTsolis UniversityofCaliforniaDavis Dr.RalphIsberg TuftsUniversity2012 Dr.DavidRudner HarvardMedicalSchool Dr.EstherAngert CornellUniversity Dr.MarkGomelsky UniversityofWyoming Dr.DennisA.Bazylinski UniversityofNevada,LasVegas2011 Dr.JamesBardwell UniversityofMichigan Dr.SeanCrosson UniversityofChicago Dr.JoHandelsman YaleUniversity Dr.R.MartinRoop,II EastCarolinaUniversity2010 Dr.PeterChristie UniversityofTexas-HoustonMedicalSchool
Dr.RichardGourse UniversityofWisconsin Dr.DanKearns UniversityofIndiana
Dr.RolfThauer MaxPlanckInstituteforTerrestrialMicrobiology,Marburg2009 Dr.JimImlay, UniversityofIllinoisatUrbana-Champaign Dr.AndyCamilli TuftsUniversity,HowardHughesMedicalInstitute Dr.JadeWang BaylorCollegeofMedicine Dr.NormanPace UniversityofColoradoatBoulder2008 Dr.RobertoKolter, HarvardMedicalSchool
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Dr.GrahamHatfull, UniversityofPittsburgh Dr.RobertoBelas, UniversityofMaryland
Dr.WilliamJacobs, AlbertEinsteinCollegeofMedicine2007 Dr.JaredLeadbetter, CaliforniaInstituteofTechnology Dr.TinaHenkin, OhioStateUniversity Dr.HarryMobley, UniversityofMichiganMedicalSchool Dr.LynnZechiedrich, BaylorCollegeofMedicine2006 Dr.DavidFigurski, ColumbiaUniversityMedicalCenter Dr.KarlE.Klose, UniversityofTexas-SanAntonio Dr.JohnHelmann, CornellUniversity Dr.VictorDirita, UniversityofMichiganSchoolofMedicine2005 Dr.SamuelKaplan, UniversityofTexasHoustonMedicalSchool Dr.GeorgeO’Toole, DartmouthMedicalSchool Dr.DianneNewman, CaliforniaInstituteofTechnology Dr.TomSilhavy, PrincetonUniversity2004 Dr.CurtisSuttle, UniversityofBritishColumbia Dr.PaulGollnick, StateUniversityofNewYork-Buffalo Dr.HeidiKaplan, UniversityofTexasHoustonMedicalSchool Dr.JorgeGalán, YaleUniversitySchoolofMedicine2003 Dr.VincentFischetti, RockefellerUniversity Dr.RobertLandick, UniversityofWisconsin Dr.BonnieBassler, PrincetonUniversity Dr.NeilWelker, NorthwesternUniversity2002 Dr.StephenFarrand, UniversityofIllinois-Urbana Dr.BarryWanner, PurdueUniversity Dr.SanfordA.Lacks, BrookhavenNationalLaboratory Dr.JanetYother, UniversityofAlabama-Birmingham Dr.MalcolmWinkler, LillyResearchLaboratories2001 Dr.KimLewis, TuftsUniversity Dr.AustinNewton, PrincetonUniversity Dr.JulieTheriot, StanfordUniversity Dr.DaleKaiser, StanfordUniversity2000 Dr.ThomasA.Trautner, Max-PlanckInstituteforMolecularGenetics Dr.RylandF.Young, TexasA&MUniversity Dr.AbigailA.Salyers, UniversityofIllinois Dr.JohnW.Costerton, MontanaStateUniversity Dr.TonyRomeo, UniversityofNorthTexasHealthScienceCenter1999 Dr.FrederickNeidhardt, UniversityofMichigan Dr.OlafSchneewind, UniversityofCaliforniaatLosAngeles Dr.RogerW.Hendrix, UniversityofPittsburghBacteriophageInstitute Dr.EugeneNester, UniversityofWashington1998 Dr.ScottHultgren, WashingtonUniversitySchoolofMedicine Dr.SusanM.Rosenberg, BaylorCollegeofMedicine Dr.MaryLouGuerinot, DartmouthCollege Dr.PeterGreenberg, UniversityofIowa
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1997 Dr.MalcolmWinkler, UniversityofTexasHoustonMedicalSchool Dr.JohnBaross, UniversityofWashington Dr.PhilipHarriman, NationalScienceFoundation Dr.SusanGolden, TexasA&MUniversity
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Welker-StreipsMemorialPlenaryAddress
NeilE.Welkerleftusafterashort,butbrave,battlewithcanceronWednesday,Aug.11,2004atage72.Neil,borninDarienCenter,NYonApril21,1932,receivedhisPhDfromWesternReserveinCleveland,OHandmovedtotheChicagoareawherehewasaProfessorofBiochemistry,MolecularBiologyandCellBiologyatNorthwesternUniversityinEvanston,ILfrom1964topresent.Heisnotedforpromotingexcellenceinundergraduatescienceeducationandsupportingstudents'participationintheWindRiverConferenceeachyearinColorado.Heissurvivedbyhislovingwife,FatimaVilich,andherchildren,InesandAdisKurtovic;histhreechildren,ThomasandDouglasWelkerandJenniferBurgerandtheirspousesandchildren;histhreestepchildren,DonandJonPollackandJoAnnCarbrayandtheirspousesandchildren;hissister,ArleneCoccariandbrother,WallaceWelkerandtheirspouses,childrenandgrandchildren;andnumerousniecesandnephews.
ThispastFebruary,TheWindRiverConferenceonProcaryoticBiologylostafriendandanactivesupporter/leader.Dr.UldisStreipshadbeenthelong-timeExecutiveDirectoroftheConferenceandworkedtirelesslyfrom1968to2016tokeeptheconferencemovingforwardasaprinciplemechanismfortheexchangeofcuttingedgescienceandasaplacewherestudentswouldbeofprimaryimportance.
UldiswasBorninRiga,LatviaonFebruary1,1942.In1944,Uldis,hisparentsHugoandElenoraStreips,andolderbrotherLaimonis,fledfromtheirhomelandduetowar.Withnowheretogo,hisfamilylivedindisplacedpersonscampsinGermanyandPolandforsevenyears.TheycametotheUSin1951andeventuallysettledinChicago.HegraduatedValparaisoUniversityin1964withaB.A.inBiologyandChemistryandthenenteredNorthwesternUniversitywhereheworkedonhisPh.D.inthelaboratoryofDr.NeilWelker(oneoftheoriginalparticipantsintheWindRiverConferencewhichwascalledTheTransformationMeetings).Uldisreceivedhisdegreein1969andthenwenttodoapostdoctoralfellowshipinthelaboratoryofDr.FrankE.Young(anotherfoundingmemberoftheTransformationMeetings)atScrippsClinicandResearchFoundation.UldiswhotrulyadoredlifeinSanDiegowasdealtacrueltwistoffatewhenFrankYoungacceptedtheChairmanshipoftheDepartmentofMicrobiology&ImmunologyattheUniversityofRochester,SchoolofMedicineandDentistry.UldishadtoturninplayingvolleyballintheeveningsonthebeachfortheweatherofupstateNY.Luckily,UldiswasrewardedbymeetinghisfuturewifePatriciaWilliamswhoworkedintheDepartmentinRochester;heimmediatelystoppedcomplainingabouttheweather.In1972,UldisbecameanAssistantProfessorofMicrobiologyattheUniversityofLouisville,MedicalCenter.Becauseofhisstate-of-theartresearchandhisoutstandingcommitmenttostudents,heproceededtobepromotedtoAssociateProfessor(1978)andProfessor(1988).HeremainedatULuntilhisretirementtwoyearsago.
Uldisreceivedseveralteachingawards,aswellastheAMWAGenderEquityAward.HewastherecipientofmultipleresearchgrantsandservedasViceChairmanoftheDepartmenttwice(forover10years).Heauthored61peerreviewedjournalarticlesand75chapters,booksandproceedings.Hewasapeerreviewerandaneditor.Hiscommitmenttomedicalstudentandgraduatestudenteducationwasspecificallyrecognizedbyhisuniversity.ThiscommitmenttostudentscarriedovertotheleadershipandguidanceheprovidedduringhisentirecareertotheWindRiverConference.Hemadesurethatstudentswerealwaysfirstandcenterinallconsiderationsandplanningfortheconference.EverythingUldisdidhedidwithpassionandcommitment.Thiswasdemonstratedmostclearlybyhis
commitmenttohisfamily;hiswifePat,hisdaughterKrista,hisson,daughter-inlawandgrandchildrenKrisjans,Sarah,Anja,Zander,Nora&Maia,respectively.UldisneverforgothisLatvianheritageorisloveofmusic.HeworkedtohelpLatviabeforeandafteritgainedindependencefromtheSovietUnionandwasrecognizedforhistirelessworkbybeingmadeamemberoftheLatvianAcademyofScience.Hewasthementorto17M.S.anddoctoralstudentswhotrainedunderhimwhilehewasatUL.Healsoprovidedguidancetocountlessnumberofmedicalstudents.For40years,Uldiswasaproud,loyalbassguitarist,vocalist,andcomedianforthebandCikagasPiecisi,asit
grewfromitsLatvianrootsintointernationalfame.Asmentionedabove,thispastFebruarywelostagreatfriend,scientist,teacher,mentor,LatvianScholar,musicianandmostofallawonderfulhusband,fatherandgrandfather.Uldiswastrulyamenschandheismissed.
Wednesday,June5,20193:00-6:00PM
Registration
4:00-6:30PM
WelcomeReception(Lobbyofmainlodge)
6:30-8:00PM
Dinner&Openingremarks
8:00-9:00PM
KeynoteAddress:
GoverningandorganizationalprinciplesofgeneexpressionDr.ChristineJacobs-Wagner
YaleUniversityHowardHughesMedicalInstitute
WestHaven,CT
SessionConvener:CassandraNelson
9:00PM- HappyHourattheloungeandbar
Thursday,June6,20197:00-8:15AM
Breakfast(GreatRoomDiningRoom)
8:15AM8:30–8:45AM8:45-9:00AM9:00-9:15AM9:15-9:30AM
GeneralAnnouncementsMicrobialBiochemistryandPhysiologyISessionConvener:AmberConsulEffectsofP.aeruginosa2-heptyl-4-quinoloneN-oxide(HQNO)onglobalironregulationinS.aureusLukeK.BrewerUniversityofMarylandBaltimore,Baltimore,MD,USAUnderstandingcorrinoidsalvaginginhyperthermophilicArchaeaElizabethA.VillaUniversityofGeorgia,Athens,GA,USACyclicdi-GMPcontrolsstresstoleranceandcellshapeinVibriocholeraeNicolasL.FernandezMichiganStateUniversity,EastLansing,MI,USA
PolarorganelledevelopmentproteinPodJisimportantforthesynthesisofoneofthetwodistinctunipolarpolysaccharideadhesinsinAgrobacteriumtumefaciensMaureenC.OnyeziriIndianaUniversity,Bloomington,IN,USA
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9:30-9:45AM9:45-10:00AM
LossofYggSinducesaconditionalaspartatelimitationinaspCstrainsofSalmonellaentericaHuongN.VuUniversityofGeorgia,Athens,GA,USA
Alinkbetweenhigh-affinityzinctransport,capsuleproduction,andgrowthofextraintestinalpathogenicEscherichiacoliinbileMichaelOlsonBrighamYoungUniversity,Provo,UT,USA
10:00-10:30AM CoffeeBreakMicrobialBiochemistryandPhysiologyIISessionConvener:KellieKing
10:30-10:45AM10:45-11:00AM11:00-11:15AM11:15-11:30AM11:30-11:45PM11:45-12:0012:15-1:15PM
ThestressoftransitioningtomethylotrophyJannellV.BazurtoUniversityofMinnesota,St.Paul,MN,USAResponseofPseudomonasaeruginosatocalprotectin-mediatedmulti-metalsequestrationCassandraE.NelsonUniversityofMarylandBaltimore,Baltimore,MD,USAMinsystemdisassemblesFtsZatcellpoleinBacillussubtilisYuanchenYu
IndianaUniversity,Bloomington,IN,USABiochemical characterization of the cyclic-GMP-AMP dependent activation of thephospholipaseCapVGeoffreyB.SeverinMichiganStateUniversity,EastLansing,MI,USACobamidesynthaseanchorsthelatestepsofcobamidebiosynthesistothecellmembraneVictoriaL.JeterUniversityofGeorgia,Athens,GA,USADegenerateformaldehydeoxidationpathwaysinMethylobacteriumextorquenscausedifferentialepistaticeffectsbetweenmutationsaccumulatedduringexperimentalevolutionEricL.BrugerUniversityofIdaho,Moscow,ID,USALunch(GreatRoomDiningRoom)
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1:30-1:45PM1:45-2:00PM2:00-2:15PM2:15-3:45PM
ResponsestoEnvironmentalStimuliandEcologySessionConvener:GarettBondsEnterococcusfaecalis:TakingupfatfromitshabitatRachelD.SteeleUniversityofTennessee,Knoxville,TN,USALegume-rhizobiasymbioticoutcomeasdeterminedbyaninterkingdompeptide-peptidasecontestAlexB.BenedictBrighamYoungUniversity,Provo,UT,USAUnderstandingthestresstoleranceofSaccharomycescerevisiaestrainsevolvedforhighsolidspinefermentationsOliviaA.ThompsonUniversityofGeorgia,Athens,GA,USA15LightningTalks:Fast-pacedpresentationstowhetyourappetitefortheupcomingpostersessions.
3:45-4:30PM4:30-6:30PM6:30-7:30PM8:00-9:00PM9:00-
FreeTime&PosterSet-upPosterSession&HappyHour(Oddnumberedposters)Dinner(GreatRoomDiningRoom)
KeynoteAddress:MappingtheChlamydiatrachomatisinclusionmembranewithproximitylabeling
systemsDr.ElizabethRucks
UniversityofNebraskaMedicalCenterOmaha,NE
SessionConvener:GeoffreySeverin
HappyHourattheloungeandbar
Friday,June7,2019
7:00-8:15AM
Breakfast(GreatRoomDiningRoom)
8:15AM
GeneralAnnouncements
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8:30–8:45AM8:45-9:00AM9:00-9:15AM9:15-9:30AM9:30-9:45AM9:45-10:00AM
GeneRegulationandBacterialGeneticsISessionConvener:RaevenBastockInvestigatingthenon-canonicalroleofthetranscription-couplingrepairfactorondisulfidestressinBacillussubtilisHollyA.MartinUniversityofNevada,LasVegas,NV,USATranscriptomicprofilingofbacteriophage-hostinteractionsrevealsphagemediatedquorumquenchingofEnterococcusfaecalisAnushilaChatterjeeUniversityofColoradoDenver,Aurora,COUSAMini-Tn7vectors,universaltoolsforgeneticmanipulationofGram-negativebacteriaHerbertB.SchweizerUniversityofFlorida,Gainesville,FL,USA
Thecyclicdi-GMPdegradationproductpGpGactivatesvirulenceinErwiniaamylovoraBrianY.HsuehMichiganStateUniversity,EastLansing,MI,USAThe activation of the extracytoplasmic function (ECF) σ factor, σP, is a regulatedprocessthatoccursinresponsetothepresenceofβ-lactamsKelsieM.NautaUniversityofIowa,IowaCity,IA,USA
Anovelhigh-throughputmethodforidentifyingmetabolicpathways,Metabolite-coupledTn-Sequencing(MetSeq),revealsnewironandhemeregulationinPseudomonasaeruginosaAndrewT.UlijaszLoyolaUniversityChicago,Maywood,IL,USA
10:00-10:30AM CoffeeBreakGeneRegulationandBacterialGeneticsIISessionConvener:LukeBrewer
10:30-10:45AM
Identification,characterizationandcomparisonofRNAthermosensorsinShigelladysenteriaeandStaphylococcusaureusErinR.MurphyOhioUniversityHeritageCollegeofOsteopathicMedicine,Athens,OH,USA
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10:45-11:00AM11:00-11:15AM11:15-11:30AM11:30-11:45PM11:45-12:0012:00-12:15PM12:30-1:30PM1:30-4:30PM
SwrAregulatesswarmingmotilitybyactivatingmotilityrelatedgeneswithintheDegUregulonAyushiMishraIndianaUniversity,Bloomington,IN,USATheroleoftranscriptioninpilinantigenicvariationofthepathogenicNeisseriaHankSeifert
NorthwesternUniversityFeinbergSchoolofMedicine,Chicago,IL,USADissectingtheregulationofthePseudomonasaeruginosahemeassimilationsystem:AlinkbetweenhememetabolismandregulationSusanaMouriñoUniversityofMaryland,Baltimore,MD,USA
H-NSfamilymembersMvaTandMvaUregulatethePseudomonasaeruginosatypeIIIsecretionsystemEmilyA.WilliamsMcMackinUniversityofIowa,IowaCity,IA,USACharacterizationoftheiron-andheme-responsivesRNAPrrHinPseudomonasaeruginosaTramyHoangUniversityofMaryland,Baltimore,MD,USAPhotograph!Lunch(GreatRoomDiningRoom)Freetime
4:30-6:30PM6:30-7:30PM8:00-9:00PM9:00-
PosterSession&HappyHour(Evennumberedposters)Dinner(GreatRoomDiningRoom)
KeynoteAddress:Pterinitup:Howasmallobscuremetabolitecontrolssurfaceattachmentby
AgrobacteriumtumefaciensDr.ClayFuqua
IndianaUniversityBloomington,IN
SessionConvener:ElizabethVilla
HappyHourattheloungeandbar
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Saturday,June8,20197:00-8:15AM8:15AM
Breakfast(GreatRoomDiningRoom)GeneralAnnouncementsHost-PathogenInteractionsSessionConvener:EmilyMcMackin
8:30–8:45AM8:45-9:00AM9:00-9:15AM9:15-9:30AM9:30-9:45AM9:45-10:00AM
EnterococcalpolysaccharideantigenimpactstransmissionfrommothertoinfantCydneyN.JohnsonUniversityofColoradoAnschutzMedicalCampus,Aurora,CO,USAAn‘openchannelconfirmation’VirB10deregulatesintracellulartraffickingandvirulenceofBrucellasuisDavidO’Callaghan
UniversitédeMontpellier,Nimes,FranceTheroleofbiofilmformationandpolyamineutilizationincolonizationofzebrafishbyVibriocholeraeGarettBondsAppalachianStateUniversity,Boone,NC,USAEvolutionofPseudomonasaeruginosainachronicburnwoundmodelErinS.GloagTheOhioStateUniversity,Columbus,OH,USAElucidatingthehealthyratoralbacteriomeandmycobiomeSaredaT.J.SchrammTheUniversityofTexasatDallas,Richardson,TX,USADiscoveryandcharacterizationofnovelsmallregulatoryRNAsinBrucellaabortusKellieA.KingVirginiaTech,Blacksburg,VA,USA
10:00-10:30AM10:30-10:45AM
CoffeeBreakCoordinatedBacterialBehaviorsSessionConvener:DavidGlanvilleAlginate-associatedproteinsinPseudomonasaeruginosabiofilmsHollyM.JacobsUniversityofWashington,Seattle,WA,USA
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10:45-11:00AM11:00-11:15AM11:15-11:30AM11:30-11:45PM11:45-12:00PM12:15-1:30PM
Geneticdeterminantsofsyntehtic4-oxazolidinonesmallmoleculebiofilminhibitioninSalmonellaTyphimuriumKyleF.GriewischNorthCarolinaStateUniversity,Raleigh,NC,USANutrient-dependenteffectofOprFonPseudomonasaeruginosabiofilmformationBooShanTseng
UniversityofNevadaLasVegas,LasVegas,NV,USAIdentificationofthebindinginterfacebetweenthebiofilmregulatoryproteinsNspSandMbaAinVibriocholeraeErinC.Young
AppalachianStateUniversity,Boone,NC,USA
ExploringspatialdynamicsofantibiotictoleranceinthecysticfibrosislungSophieE.DarchUniversityofSouthFlorida,Tampa,FLUSAThedynamicsofbiofilmmatrixassemblyinthemodelorganismPseudomonasaeruginosaCourtneyReichhardtUniversityofWashington,Seattle,WA,USALunch(GreatRoomDiningRoom)
1:30-4:30PM4:30-6:30PM6:30-7:30PM8:00-9:00PM9:00-
FreeTimeHappyHourDinner(GreatRoomDiningRoom)
THEWELKER-STREIPSMEMORIALPLENARYADDRESS
Cyclicdinucleotides:FromcholeratocancerDr.ChristopherWaters
MichiganStateUniversityEastLansing,MI
SessionConvener:MartyRoop
HappyHourattheloungeandbar
7:00-8:30
Sunday,June9,2019
Breakfast(GreatRoomDiningRoom)Departure