Supplemental Figure 1
Mock MOI-10 MOI-1000
5
10
15
20
25
1.45
6.16
19.74
[18F]-FHBG Uptake in NY-ESO-1-TCR/sr39TK Transduced Jurkat Cells
No
rma
lize
d U
pta
ke
EC50 of Jurkats with Ganciclovir
10- 1 5 10- 1 2 10- 9 10- 6 10- 3
0
20
40
60
80
100
EC50Mock
0.0002888MOI-10
6.370e-011MOI-100
8.454e-011Medium Only0.0004830
R squareMock
0.9483MOI-100.9773
MOI-1000.9727
Medium Only0.9144
MockMOI-10MOI-100Medium Only
[Ganciclovir] (M)
% V
iab
le C
ells
(N
orm
aliz
ed)
C
B
A
NY-ESO-1 Tetramer PE
CD
3 P
erC
P
79.7%21.8%0.5%
Mock MOI-10 MOI-100
B
Supplemental Figure 2
Thymus
L Humerus
Muscle
L Femur
R Humerus
R Femur
A
In Vivo [18F]-FHBG Uptake
% I
D/g
(b
kg
d s
ub
tra
cte
d)
Mock ESO/TK
0
2
4
6
8P < 0.0001
Non-Transduced Humanized
% ID
/g (
bkg
d s
ub
trac
ted
)
NT1 NT2 NT3 NT1 NT2 NT3
0
2
4
6
8
C
Supplemental Figure 3
V1
V2
V3
V4
V5
V6
V7
V8/9
0 5 10 15 20 25 30 35 40 45 50
ψ Neg ψ Pos
%hCD45
A B
