These images illustrate results of in situ hybridization with eighttomato BACs. Putative locations for the BACs are included in the captions. All of these experiments were performed without CISShybridization. On the basis of these results, two of the BACs (045N22,098L02) appear to contain only single-copy sequences. The other sixcontain multiple-copy sequences present either predominantly in thepericentromeric heterochromatin of all of the chromosomes (007K09,098L02, 251G05, 060G11) or at multiple telomeric locations (209L16,216M19). One of the BACs having multiple-copies sequences in thepericentromeric heterochromatin (177F12) also has a locus ofhybridization at the telomere of the long arm of Chromosome 2.We are currently performing additional experiments with these BACsusing CISS hybridization with Cot100 tomato genomic DNA to determinewhether their single-copy sequences can be located specifically.

Stack Lab--April 8,2005

045N22 (Chromosome 12—Telomere P)

098L02 (Chromosome 6—Telomere Q)

007K09 + 251G05 (both Chromosome 6--Centromere)

007K09 251G05

Same SC spread as previous slide

007K09 (FITC, Chr. 6--Centromere) + 177F12 (TRITC, Chr. 2—Telomere Q)

Arrow indicates label at telomere of long arm of Chromosome 2

Chr. 2

007K09 177F12

Same SC spread as previous slide

251G05(Chromosome 6- Centromere)

060G11 (Chromosome 2—Telomere P)

216M19 (Chromosome 8—Telomere P)

209L16 (Chromosome 3—Telomere P)