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Indus Valley electron microscopy and Crystallography

Abstract

The Indus Valley Civilization was an ancient civilization located in what is Pakistan and northwest

India today, on the fertile flood plain of the Indus River and its vicinity. The history of ancient

India is glorious one with Vedic symbols on the seals as evidences of their achievements. The

electron microscopic structures of DNA and crystallographic images on the seals explore their

advanced technology. It was a Vedic civilization with Vedic symbols on the seals.

The quest of creation is quest of human mind from dawn of the Civilization on the earth.

The discovery of the double helical structure of the DNA by the Watson and crick 1953

has brought the revolution in the field of Biotechnology. The same echo is coming from

the scientifically developed Indus Valley Vedic Civilization.

A pictographic symbol is worth than one thousand words. It has been used on the seals

with electron microscopic cellular structures and crystallography images on the seals

The pictographic symbols on the seals are cytological models for teaching and

demonstration; the symbols explore the cellular genetic evolution on the earth with

genetic recombination. Its literary evidences are in the Vedic hymns.

The Vedic metaphoric symbols for DNA have been engraved on the seals.

Accordingly the seals and cytological symbolic pictographs deciphered in

chronological order. It is not a script of known language but microscopic cytological

models. The electron microscopic structure of DNA engraved on the seals confirm

their advance technology, it explore evolution of human species in chronological

order.

Electron microscopic structures of DNA & crystallog raphy images on the

seals explore their advanced technology.

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1. The synthesis of chromosome 2. DNA replication and Protein

translation

3. Nucleotide pairs 4 Nuclear reaction – the ribosome translates the DNA

code into life.

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Prof. Chandra P Trivedi

Prof. Chandra P Trivedi Professor with incredible amount of experience as professor, researcher and Principal of lead college, Bhind and Ratlam under Jeewaji University, Gwalior, and Vikram University, Ujjain respectively.

As an academician author of more than 12 books, his books & work has been published in many leading papers of the Subcontinent & books from India and Germany

He is former senior fellow, Department of culture, Govt. of India, Associate IIAS, Shimla. As a Professor and Scientist, he has gone into the depth of Yoga, Meditation, Spiritualism and Vedas at Vedic Research Institute, Ratlam. He is among original thinker with innovative approach to solve the national problem. He explored India’s glorious past with answer of Einstein’s Grand Unified Force. As an academician, Vedic Scientists and professor, he has first time crack the Vedic code with oldest un-deciphered symbols of the world ‘Advanced gene technology’.

Going ahead Prof. Trivedi has given the answer of modern problems related with origin of life consciousness and Higgs field 2012 The Vedas preserved over the centuries with its musical tones from generation to generation, it explore the secret of creation that each generation is new, but the voice is immortal, which follow the mankind from generation to generation with new Life.

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Indus Valley electron microscopy and Crystallography

Introduction

The Indus Valley Civilization was an ancient civilization located in what is Pakistan and northwest

India today, on the fertile flood plain of the Indus River and its vicinity. The history of ancient

India is glorious one with Vedic symbols on the seals as evidences of their achievements. The

electron microscopic structures of DNA and crystallographic images on the seals explore their

advanced technology. It was a Vedic civilization with Vedic symbols on the seals.

The quest of creation is quest of human mind from dawn of the Civilization on the earth.

The discovery of the double helical structure of the DNA by the Watson and crick 1953

has brought the revolution in the field of Biotechnology. The same echo is coming from

the scientifically developed Indus Valley Vedic Civilization.

A pictographic symbol is worth than one thousand words. It has been used on the seals

with electron microscopic cellular structures and crystallography images on the seals

The pictographic symbols on the seals are cytological models for teaching and

demonstration; the symbols explore the cellular genetic evolution on the earth with

genetic recombination. Its literary evidences are in the Vedic hymns.

The Vedic metaphoric symbols for DNA have been engraved on the seals. Accordingly

the seals and cytological symbolic pictographs deciphered in chronological order. It is

not a script of known language but microscopic cytological models. The electron

microscopic structure of DNA engraved on the seals confirm their advance technology, it

explore evolution of human species in chronological order.

Figure 1 organic evolution

Sons of Sudhanvan, after your long journeying, ye came unto the home of liberal

Savita Rig. 1-161 10

After long period of gap, the Sudhanvan - coaservates reached to the stage of a

cell.

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Figure 1 Figure 1 the animal motif is symbol for the fundamental energy and the incense

burner is symbol for the sun as a source of light and energy, the two antelope

indicate the synthesis of the DNA, and the two dots in front of the mouth indicate

origin of the coasrvates in early primitive conditions. The symbol of leaf above

the animal is the symbol for the photosynthesis and conversion of the light

energy into the chemical energy. The genomic chromosome is symbol for the

genetic material. Accordingly, with time from the Sudhanvan - coaservates a

eukaryotic cell came in to function Rig.1-161-10 the leaf is the symbol for the

nourishment, the genomic chromosome is symbol for the genetic material, and

they have opened the door of organic evolution.

Figure 1 symbol 1 the two independent dots are the symbolic Sudhanvan –

coaservate drops, they have been produced in early primitive condition of origin

of life in the water.

Figure 1 symbol 2 the antelope symbol expresses the synthesis of the

genetic material - two helix of DNA.

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Figure 1 symbol 3 the leaf is a symbol of photosynthesis and

nourishment, & the U shaped genomic chromosome, symbolically it has been

expressed that they together generated a eukaryotic cell, and accordingly they

have open the door for the organic evolution on the earth.

Figure 2 - Evolution of the creation – A group of t iny seals

Figure 2 the animal motif symbol is imaginary fundamental energy in the

cosmos,

The animal symbol on the seal shows evolution of the Creation from the

fundamental energy, the imaginary animal motif is symbolic expression for the

fundamental energy in the cosmos. The incense burner below the neck is symbol

of light and energy, which indicate sun as the source of energy and light on the

earth. Above the animal motif the origin of the biotic and a-biotic components has

been indicated by the bifurcation of the hollow structure in the two different

directions.

Figure 2 symbol 1 a above the animal seal, the button seal indicate origin of the

biotic and a-biotic components of the nature. It has been indicated by the

bifurcation of the hollow structure in the two different directions.

Figure 2 symbol 1, 2, 3 four horned buffalo emitted

2 symbol 1 the symbol with four dots on the four corners and the cross The

four dots on four corner is the symbol for the generation of four components of

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the atom (electron, proton, neutron and the generation of the energy in the atom),

which constitute the base of the creation, the four lines in the square is the

symbol for the four bases (Adenine, Guanine, Thymine, and Cytosine), which

constitute the base of the DNA and the living-beings. Rigveda 4 - 58 1-2-3 four

horned buffalo emitted.

Figure 2 symbol 2 DNA code into life

The next symbol with a fork and two hanging loop, The fork is symbol for the

DNA replication and loops represent the ribosome, it express that the ribosome

translates DNA code into life.

2 symbol 3 DNA duplication - the symbol is ladder like model of DNA

with nucleotide pairs.

Figure 2 symbol 5 to 8 role of messenger RNA

5 DNA replication and transcription

6 mRNA

7 DNA replication

8 inversion of the chromosome with formation of new chromosome.

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The symbols on the miniature show the role of messe nger RNA in DNA

replication for the synthesis of the new chromosome . The chromosome

under go inversion, which leads to evolution.

DNA transcribe the message into the mRNA, it has been shown by single strand

of DNA on one side. The mRNA have the message for the DNA replication .

The replicating DNA synthesize chromosome, the chromosome undergo

inversion ., which leads to evolution in nature.

.

Figure 2 symbols 9 to 12 gene interaction

9 the genomic chromosome

10 DNA replication

the symbol is for the DNA replication, through which they synthesize the gametes

during the sexual reproduction.

11 the cell division, first division and second division,

12 gene interaction

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The symbols show the stages in the evolution of the living-beings. The

development of the physical body is due to the mitotic and meiotic cell division.

The gene interaction decide the trend of evolution.

Figure 2 symbols 13 to 17 evolution of human-being

13 the symbol is for the triplet code on the messenger RNA, which carry the

message for evolution.

14 the DNA replication

15 the symbol of the genomic chromosome with gametes

16 crossing over in the cell

The chromosomal crossing over in the cell is important for the evolution in the

nature, with generation of the two different types of the DNA in the cell body.

17 the two types of the DNA for the synthesis of XX & XY chromosome

The two similar symbols of the DNA replication, showing the role of the two

double bond, and a triple bond of the hydrogen in replication. The triple bond is in

the centre of triplet nucleotide pair. The two different DNA types of DNA in the

womb synthesize the two different types of the chromosome XX and XY, it has

been expressed by two similar DNA.

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The evolution of the living-beings in the nature is regulated by the triple bond of

the hydrogen. The chromosomal crossing over leads to evolution of the different

gametes, and the synthesis of the Genomic chromosome. Accordingly, the

human-species has evolved on the earth.

18 the evolution of human species.

19 the genomic chromosome

The genomic chromosome synthesize the two different types of the chromosome

XX and XY,

20 the human figures express the evolution of male and female from the XX

and XY chromosomes and the gametes.

21 the gametes

22 the humped bull indicates symbolic fundamental energy and the two

horns indicate the origin of the double helical DNA from the fundamental energy.

The human figures expresses the evolution of male and female.

Figure 3 Cell division metaphase stages

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Figure 3 - 1 the chromosome showing chromonemata

2 Modern model of the chromosome

3 the metaphase stage of cell division

4 Chromosomal crossing

3 the DNA nucleotide pairs showing replication

Figure 4 Meiotic crossing over

Figure 4

1 the chromosomal crossing over during the meiotic cell division.

2 the modern model of crossing over

3 the DNA replication

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4 the recombinant DNA, during this the genetic material is broken and then

joined to different DNA.

The chromosomal crossing over is the exchange of genetic material between

homologous chromosomes. This often occurs during prophase stage. Crossing

over was first described in theory by Thomas Hunt Morgan.

Figure 5 Synapsis formation

The figure shows the stages during the meiotic crossing over, during this the

genetic material is broken and then joined to different DNA. During the crossing

over the homologous chromosomes come close to form the pair, it is synapsis,

then after pairing the two chromosomes surrounded by protein structure form

synaptonemal complex , and separated with recombination.

Figure 5 1 pairing of homologous chromosome synapsis

2 synaptonemal complex.

3 chiasmata formation

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4 recombination

5 the modern model of the crossing over with figures on the seal for the testimony of the present finding.

6 the crystallography image of DNA, showing DNA replication on either side.

Figure 6 the tetrahedral arrangement of Hydrogen bo nds

Figure 6 the tetrahedral hydrogen bond and water molecule

The nuclear reactions are driven by the tetrahedral arrangement of the Hydrogen

bond and the water molecule. It has been shown by the hexagonal model in the

beginning. The functions of the Nucleic Acid is shown in series on the seal.

1 tetrahedral arrangement of hydrogen bonds about each water molecule

2 the modern snowflake model to show the arrangement of

hydrogen bonds about each water molecule.

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The tetrahedral hydrogen bond and water molecules are important for the

nuclear reactions. It has been demonstrated by the modern crystallographers.

The hexagonal symmetry of snowflake results from the tetrahedral hydrogen

bonds about each water molecule. The water molecules are arranged similarly to

the Si02. The resulting crystal structure has hexagonal symmetry when viewed

along a principal axis.

Nucleic Acid – functions

3 The ribosome regulate the DNA code into life

The ribosome attached with the replicating DNA to translate the DNA code into

life, and mRNA carries the message for the protein synthesis and the DNA

replication.

4 the messenger RNA carries the message for the protein synthesis.

5 the DNA replication

6 the chromosomal crossing over.

7 the genomic chromosome

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Figure 7 DNA replication & insertion of gene

Figure 7

The symbolic model of fundamental energy; The feeding trough in the front

expresses that the components of the creation are synthesized from the

fundamental energy and merge into the same. The bifurcating horns express the

DNA treplication with projected nucleotides. The insertion of the foreign gene has

been demonstrated by a loop like structure in between the replicating strips on

the neck symbolically.

1 the crossing over with insertion of gene

2 the gene

3 the instrument for the insertion of gene

4 insertion of gene

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5 the genomic chromosome

6 the gene modification

Figure 8 Synthesis of genomic chromosome pair

Figure 8 the cell is in the centre of all the nuclear reaction and synthesis of two

types of chromosome. The formation of the gametes and the DNA replication are

the source of evolution.

1 two types of the chromosome

2 the symbolic DNA replication

3 the cell with nucleus in the centre

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4 the genomic chromosome with gametes

5 the two replicating DNA with a bridge

Figure 9 Synthesis of chromosome from the chromosom e

The seal shows the synthesis of the chromosome from the

chromosome in the cell with formation of the gametes and DNA replication.

1 the extension series of the chromosomal thread

. The chromosome synthesize the chromosome in the end of cell division.

Figure 10 Packaging of DNA

10 a & b

10 a

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Figure 10

1 mRNA

2 the Ribosome translate the DNA code into life

3 c DNA

4 duplicating DNA

5 DNA Electron microscopic model showing spiral bands of DNA

6 modern model of the chromosome packaging

7 Modern model of the DNA duplication

Figure 10 b. Showing Packaging of DNA – solenoid

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10 b The nucleosome bead necklace gradually assumes a coiled form called a

1solenoid, through coiling it produces super coiled condensed DNA. It has

been depicted on the seal.

1 1 2 3 4 5

Figure 11 Octamer – nucleosome bead necklace

The chromosomes are composed of chromatin, it is DNA and protein.

The chromosomal protein play major role in packaging of DNA. During

this process they have observed the bead like structure with string and

octamer, around which the DNA wrapped. The nucleosome bead necklace

gradually assumes a coiled form called a solenoid.

Figure 11 a b.

Figure 14 7 A model of octamer bead necklace b. figure on the seal had been

excavated along with group of seals figure 14

Figure 12 implantation of gene in the womb

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Figure 12 the half animal and a female human figure is symbol

of genetic recombination through implantation of a modified gene from out side,

on the head two curved horn is a symbol for the two helix of DNA, which

produces long chromosome fibril, it has been expressed by the emerging fibril

from the head, in the series chromosomal inversion and RNA with nucleotide has

been depicted, the implantation of the gene has been expressed by the injection

like tube above the hand, and by the mark on the hip of the animal, the position

of ovary has been indicated to implant the gene in the womb.

1 thread like chromosome fibril from the DNA replication

2 chromosomal inversion

3 the symbol of single strand of DNA with projected nucleotides.

4 the symbolic implantation of gene

5 figure showing the position of the ovary

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Figure 13 Modern model of DNA showing extension of DNA fibril from the

chromosome

The structure of DNA as we know today.

Figure 14 The Nucleic Acid study

Figure 14 Groups of tiny seals, sealing & button seals – Nucleic Acid study fine

images

The Nucleic Acid study in the cell requires specific techniques and instruments.

With the discovery of electron microscope and X ray diffraction crystallography,

the secret of DNA has been explored. The seals show the similar stages of

experience in revealing the atomic structure of matter and DNA. Without this the

metaphoric structure of the atom & DNA ‘four horn buffalo emitted’ will remain a

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slogan without the knowledge of electron microscopy and X ray diffraction

crystallography.

The images on the tiny seals are hard to understand, but the skill with which

these seals are prepared, it must have some extra importance. Accordingly with

deep studies the seals have revealed the images, they are very much near to the

modern electron microscopic and x ray crystallography images. We have only the

images, their exact chemical name or name of protein can not be traced due the

difference in terminology. We can compare the images on the computer with

reference to the context of the figures on the seal, The figure of loop pliers and

the robot has been depicted on the seal, besides two images are related with

protein synthesis, three images with implantation technique, and two images are

related with the crystallography.

Figure 14 group of seals figure discussion

Figure 14 1 Micro Loops

a b c

Figure 14 1 Each box contains 20 MicroLoops E™ atta ched to stainless steel rods.

Choose between Vertical, Horizontal and Inclined ap erture MicroLoops E™.

MicroLoops used in modern technique

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MicroLoops E™ are precision attached to beveled, so lid, nonmagnetic

stainless steel rods with standard lengths of 11, 1 9 and 25 mm and SPINE

length. They can be cut to other lengths using spri ng steel compatible

cutters . Rods can be easily bent using needlenose pliers t o place the

crystal in a desired orientation. The same has been indicated symbolically

Figure 14 2 Robot

The Scripps Institute’s Williams was sometimes able to work by remote control

from La Jolla using the advanced automation at the SIBYLS end station, whose

robots and software can load samples, track ongoing experiments in real time,

and collect data.

14 2 the robot on the seal

Figure 14 3 The placement of the crystal in desired direction

Figure 14 3 a. b. c.

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The successive steps have depicted with single crystal and the needlenose plier

in the hand of a man, the triplet has been depicted symbolically with three head

of the animal symbolically. In succession the crystal modify the DNA, it has been

indicated by the growth of the horns and a plant like structure in between two

symbolic head of the animal.

Lord of the dwelling, he subdued the demon who roared aloud, six eyed and triple headed. Trita made stronger by the might he lent him, struck down the boar with shaft whose poison

was iron. Rg.10-99-6

Lord of the brave Indra cleft him in pieces, who sought to gain much strength and deemed him mighty. He smote his three heads from his body seizing the cattle of the omniform Tvastr. Rg. 10-8-9.

Figure 14 4 Crystallography image

Franklin has reported that DNA existed in two forms. These forms are the

dehydrated is A form and the fibrous structure due to humid condition is B form.

The A and B forms were then separated and subjected to X-ray crystallography

obtaining the pictures which would be the basis of Watson and Crick’s helical

DNA structure; one of them the famous photo of the B DNA

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Figure 14 4 a. crystallography image of A form 14 4 b modern model of A form

DNA

.

14 4 c Crystallography image of B DNA 14 4 d the figure on the seal

The seal symbol shows the crystallography image of the DNA, along with the

infection of tryptophan gene to obtain phage genome

X-ray crystallography, was the means to discover the molecular structure of DNA

1952, Rosalind Franklin, produces an x-ray photograph of DNA that proves it has

a double helical structure.

14 - 5 Astbury’s image from X Ray studies of Nucle ic Acids

a.I.. II. b

Figure 14 5 A I, II . Astbury's images, from "X-Ray Studies of Nucleic Acids," 1947

14 5 bThe same studies have been depicted on the steatite button seal

Figure 14 6 Protein translation

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a b

Figure 14 6 a Protein translation b. on the seal

Figure 14 7 DNA copying and protein synthesis

a. a b.

14 7 a modern model The structure on the seal

Figure 14 8 Crystallography of Protein

A b figure on the seal

-

Figure 14 8 A b figure on the seal

Figure 14 9 The two dimensional Electron crystallog raphy,

the typical tetrameric structure of aquaglyceroporin is up to a comparable level

on the seal.

Figure 14 10 2 D crystal image of DNA

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Figure 14 10 a 2 D crystal image of DNA figure 14 9 b on the seal

Two 2D crystal image of DNA has been given by R. Franklin & Gosling 1954

Figure 15 DNA Repair Protein at Work

It has been shown that two Mre11 proteins worked together as a dimer, forming a

U-shaped structure for the DNA repair called homologous recombination. For

repairing the protein bind with DNA.

Figure 15 A U shape of the Mre11 dimer, F 15 b the symbol on the seal

is rare, it has been given with recombinant DNA, it indicate the importance of the

symbol of DNA, the black lining at the border may be protein.

Figure 16 A group of Seals - Nucleic Acid study at omic resolution images Atomic resolution images The X ray crystallography has helped in the study of atomic structure of atom.

The tetrahedral arrangement of the hydrogen bonds has given important clues

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for the study of nucleic acid. The similar experience of studies and atomic

resolution images can be observed on the tiny seals.

Figure 16 A groups of seals – Nucleic Acid study

Atomic resolution images 1, 2 & 3

Figure 16 1 a b crystalline solid atomic image of Strontium titanate.

A b

Figure 16 1A Crystalline solid atomic image of Strontium titanate the brighter

atoms are Sr and darker are Ti b on the seal

Figure 16 2 and 16 3 Square and Hexagonal image

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16 2 A Square image b on the seals

16 3 A snowflake crystal hexagonal image b on the seal

Drawing of square (Figure A, above) and hexagonal (Figure B, below) packing

from Kepler's work, Strena seu de Nive Sexangula.

Work Strena seu de Nive Sexangula (1611) that the hexagonal symmetry of

snowflake crystal was due to a regular packing of spherical water particles.

As shown by X-ray crystallography, the hexagonal symmetry of snowflakes

results from the tetrahedral arrangement of hydrogen bonds hydrogen bonds

about each water molecule. The water molecules are arranged similar to the

silicon atoms in the tridymite polymorph of SiO2. The resulting crystal structure

has hexagonal symmetry when viewed along a principal axis.

Acknowledgement

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I am very much thankful to the Department of Culture, Government of India for

the Grant of Senior fellowship, to the Indian Institute of Advance Study, Shimla

for the associate-ship, & Govt. of M.P. Higher education for the opportunity to

work. I feel great honor for honorable late P.M. Shree Rajeev Gandhi for his

timely encouragement. I am thankful to Dr. R.R. Das Ex V.C. Gwalior, Dr.

S.N.Rath Ujjain for the guidance, and Dr. Bhagirath Prasad Ex V.C. Indore for

encouragement. I am also thankful to the Archaeological survey of India &

Harappa.com for the Indus valley seals and the tablets.

Prof. C.P. Trivedi, 109,111, C-Block, Sagar Apt. Former Senior Fellow, Department of Culture, Govt. of India, 17/1, Old Palasia

Associate IIAS Shimla, Former P.G. Principal, Govt. of M.P. Indore - 452001 Mobile 094254 56518, Ph.0731-2544436

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