iCell ME150 Laborimpex€¦ · Patient Info. Cell Info. Operation Items. 3. Product Detail 3.7...

transcript

iCELL® ME‐150

LABORIMPEX SAadrian.craciun@laborimpex.be

OUTOUT‐‐LINELINE

Product Introduction

Application Background

Product Detail

Principle Overview

1. Product IntroductionIVD Equipment：Automated Blood Cell Morphology Analyzer (ME-150)

Blood Smears(INPUT)

ME-150 Digital Images & Report(OUTPUT)

Clinical Lab. Dept.

HematologyDept.

PathologyDept.

2. Application Background2.1 Blood Routine Examination

70%~80%Samples

20%~30% Samples ( ISLH 41 Rules, 2005 )

Report

Manual Morphology Analyzer

Screening Test

Diagnostic Test

2. Application Background2.2 Manual Differential Cell Analysis：dilemmaLabor‐intensive, time‐consumed

Supposed 1000 samples/day;

Abnormal for morphology check: 200-300 smears/day.Skilled technologist spends an average of 6 minutes/smear for morphology;Total time : 20-30 hours/day！！！

Lack of standards for subjectivity

Inconvenience for storing or sharing sample information

WE NEED AUTOMATED MORPHOLOGY ANALYZER!!

2. Application Background2.3 Development Review for Morphology Analyzer

1980s 1990s

2000s1970s

Semi-Automation

1. CELLSCAN/GLOPR,�Canada;2. LARC,�Corning�Electronics;3. Diff3,�Coulter;4. ADC-500,�Abbott;5. Microx,�Omron.

Microscopy Image

Judith,�Prewitt,�University�of�Pennsylvania

Color Image Capture

SAMBA-200,�Thomson-TITN Smaller Size, Better PerformanceCoulter�Gens,�Beckman�Coulter

Automation Digitizing

1. Easy�Cell�Assistant,�Medica;2. Vision�Hema,�WestMedica;3. HemaCAM,�Horn�Imaging;4. DM96 (2007) DM1200

(2009) DM9600(2014), Cellavision AB (Sweden);

5. ME-150 (2015), iCELL (China)

Microscopic Examination: Gold Standard

3. Product Detail3.1 System Components

Morphology Analyzer Workstation

MonitorMagazine & Smears

iCELL™ ME‐150

Printer

3. Product Detail3.2 Examination ItemsItems Cell Differential Counts Morphology Parameters

Band Neutrophil, Segmented Neutrophil, Lymphocyte, Monocyte, Eosinophil, Basophil, Blast cell, Promyelocyte, Myelocyte, Metamyelocyte, Atypical Lymphocyte.

Left shift, Right shift, Toxic granulation index, Lobulation index, Vacuolation, Mean area, Mean diameter, Area variant, Diameter variant.

Non‐WBCErythroblasts(NRBC), Giant thrombocytes,

Thrombocytes aggregations, Smudge cells and Artefacts

Erythrocytes, Microcytes, Macrocytes, Gigantocytes, Hypochromic erythrocytes, Hyperchromic erythrocytes, Teardrop cell, Elliptocytes, Target Cell, Unidentified.

Poikilocytosis, Rouleaux formation, Anisocytosis, Mean area, Mean diameter, Area variant, Diameter variant, Howell‐Jolly bodies, Pappenheimer bodies, Basophilic stippling, Parasites.

PLTSmall, normal, giant thrombocyte; Severe,

moderate and mild thrombocyte aggregations

Anisocytosis, Mean area, Mean diameter, Area variant, Diameter variant.

3. Product Detail3.3 Application Value

ISO15189:2012 CAP

Remote Diagnosis Joint Diagnosis Medical –Tech Education

3. Product Detail Test Velocity：30~35 smears/hour Image Resolution：0.13 µm/pixel（oil） Preclassification Accuracy：

3.4 Performance

MYE-meta%

MYE-pro%

Blast%

ME-150 91.7 97.1 93.3 84.3 95.5 46.7 24.5 69.2 58 77.4

DM96(UCL) 87.6 94.8 79.9 54.1 99.5 32.6 37.7 77.6 76.6 80.6

DM96(CUF) 92 99 96 80 95.6 58 56

UCL: University College London CHU: University Caen France

3. Product Detail3.5 Highlight

ToxicGranulation

Lobulation

Nucleolus

Precise, Accurate Evidence-based Medical

NEU-b NEU-s MON

LYM BAS EOS

Abnormal Morphology Evaluation System

3. Product Detail3.6 Hardware

Hardware(Image)

Algorithms(Morphology)

Software(User Interface)

Report

3. Product Detail3.7 Software (Analyze)

VideoPatient Info.

Cell Info.

Operation Items

3. Product Detail3.7 Software (Review)

Cell Info.

Images

SampleList

Patient Info.

3. Product Detail3.7 Software (Report)

RBC & PLT

Morphology

3.Product Detail3.8 Specification

Product ME-150（iCELL) DM96 (Cellavision AB)

LDC, WBC morphology WBC presorting

RBC morphology RBC qualitative analysis

PLT morphology PLT count

Toxic granulation Morphology Test, Quantitative Analysis None

Lens 10X/100X(Oil) 10*/50*(Oil)

Principle ANN、SVM ANN

Loading (once) 150 smears 96 smears

Max. Resolution 732×574 pixal 658×494 pixal

Emergency Function

Emergency Case Priority

Single Slide mode can be set

Data Memory Workstation (Parameter can be customized) 1G RAM, 160G ROM

4. Principle Overview4.1 Principle Outline

Monolayer PositioningMonolayer PositioningAuto-focusAuto-focus Cytological

ClassificationCytological

Classification

1.Quantitfication

Focus Function;

2. Focus Locating

3. Capture Images

1.Quantitfication

Focus Function;

2. Focus Locating

3. Capture Images

Core Algorithms

1.Quantitfication

Monolayer Function;

2. Scanning

3. Monolayer Locating

1.Quantitfication

Monolayer Function;

2. Scanning

3. Monolayer Locating

1.Image Segmentation

2. Feature Extraction

3. LDC Analysis

4. Morphology

1.Image Segmentation

2. Feature Extraction

3. LDC Analysis

4. Morphology

4. Principle Overview4.2 Leukocyte Differential Counts: Supported Vector Machine

SVM: Feature Space(Low Dimension)

Hilbert Space(High Dimension) Hyper plane

4. Principle Overview4.3 WBC Morphology Analysis

Left/Right Shift Specificity 98.9%, Sensitivity 76.7%

iCell ME150 Laborimpex€¦ · Patient Info. Cell Info. Operation Items. 3. Product Detail 3.7...

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