Kinesin-5 nano-motors in cell division Kinesin-5 nano-motors in cell division

Leah GheberLeah Gheber

Departments of Clinical Biochemistry and ChemistryIlse Katz Institute for Nanoscale Science and Technology

Ben-Gurion University of the Negev, Beersheba, Israel

Mitotic spindle

human cells – specific kinesin-5 inhibitor human cells – specific kinesin-5 inhibitor monastrolmonastrol

monastrolmonastrolcontrolcontrol5 m

Leizerman et al., CMLS 2005 (Gheber lab)

- +

+ -

Kinesin-5

Kinesin-5 motor proteins Kinesin-5 motor proteins

Spindle assemblySpindle assembly Maintenance of bipolar Maintenance of bipolar spindle structurespindle structure

Anaphase spindle Anaphase spindle elongationelongation

Our GoalOur Goal

To understand the mechanisms and regulation To understand the mechanisms and regulation of the mitotic kinesin-5 nano motorsof the mitotic kinesin-5 nano motors

Our StrategyOur Strategy

To correlate between in vivo functions and in To correlate between in vivo functions and in vitro motor activity of specific kinesin-5 vitro motor activity of specific kinesin-5

mutants and variantsmutants and variants

In vivo assays – protein expression and In vivo assays – protein expression and quantitative live imaging quantitative live imaging

Cin8-3GFPCin8

Cin8-5A-3GFPCin8-3A

Cin8

26 37 26 37

cin8-5A

Microtubule plus-end trackingTubulinKinesin-5

In vitro - Single molecule fluorescence In vitro - Single molecule fluorescence motility assay - Kinesin-5-3GFPmotility assay - Kinesin-5-3GFP

CollaboratorsCollaboratorsChristoph Schmidt – GAUGStefan Lakamper – GAUGMart Loog – Tartu, EstoniaLiam Holt – UC Berkeley, USAndy Hoyt – JHU, US Dina Raveh – BGU, Israel

NataliaNatalia

AdinaAdina

VladimirVladimir

RachelRachel

People who do the workPeople who do the work

Vladimir FridmanVladimir Fridman

Adina Gerson-Gurwitz Natalia Adina Gerson-Gurwitz Natalia

MovshovichMovshovich

Maria PodolskayaMaria Podolskaya

Rachel Abunie-MasalaRachel Abunie-Masala

Ofer ShapiraOfer Shapira

Nurit SchkolnikNurit Schkolnik

Alina GoldsteinAlina Goldstein

AlinaAlina

OferOfer

NuritNurit