Post on 17-Dec-2015
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PREPARATION OF A BACTERIAL SMEARPREPARATION OF A BACTERIAL SMEAR
SPECIAL STAINING TECHNIQUESSPECIAL STAINING TECHNIQUES
ASEPTIC PROCECURE FOR MICROBE REMOVAL
http://www.youtube.com/watch?v=0odxJy0nR9s&feature=related
http://www.youtube.com/watch?v=HxjN2mBLGCE&feature=related
http://www.youtube.com/watch?v=n-HHI4E1LhI&feature=related
STAINING SPECIAL STAINING TECHNIQUES
Negative Staining for Capsules» Capsules
• Determinants for virulence in some genera of bacteria
» Capsule Staining• Preparation of a bacterial smear along with
negative staining w/ Nigrosin or India Ink• Counterstain w/ Crystal Violet
STAINING SPECIAL STAINING TECHNIQUES
Negative Staining for Capsules» Preparing a bacterial smear for Capsule Staining
STAINING SPECIAL STAINING TECHNIQUES
Endospore Stain Genera of bacteria that produce endospores
» Bacillus & Clostridium» Other genera: Desulfotomaculum, Sporosarcina,
Sporolactobacillus, Oscillospira, Thermoactinomyces» Schaeffer-Fulton Method for Endospore Staining
• Preparation of a Bacterial Smear-Air dry• Application of Primary Dye (Malachite Green) w/
concurrent application of heat• Allow slide to cool• Rinse w/ water• Application of Counterstain (Safranin)
http://www.tgw1916.net/video_pages/Spore.html
http://www.answers.com/topic/endospore
STAINING SPECIAL STAINING TECHNIQUES
Endospore Stain Results
Flagellar Stain» Uses mordant and carbolfuchsin stain to increase
flagellar diameter until resolved by light microscope
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http://www.answers.com/topic/endospore
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