AdvancedGeneticsMar.7th 2017

YangLi

andthe

Wild-type?• Consideredinbredstrainif20consecutivegenerationsofbrotherxsistermatings

• E.g.C57BL/6J– C57BListheparentstrain– 6istheline– JistheJacksonLabcode

• LaboratoryregistrationcodesindicatewhichlabthecrosswascarriedoutinJ:TheJacksonLaboratoryMcw:MedicalCollegeofWisconsinKyo:Kyoto UniversityMit:MassachusettsInstituteofTechnology

CrossingStrains

• ProgenyofhybridcrossesdesignatedbyF1• IntercrossingtwoF1s->F2s

-D2B6F1(DBA/2motherandC57BL6/Jfather)-D2B6F2(offspringofaD2B6F1intercross)

Alleles• Namedbyphenotype• Superscripted• Italicized• Dominant=firstletteruppercase• Recessive=alllowercase– ApcMin =dominantmultipleintestinalneoplasiaalleleoftheadenomatosis polyposiscoligene

– Leprdb =recessivediabetesalleleoftheleptinreceptorgene

LociandGenes

• Firstlettercapitalized• Italicized– E.g.Apc =adenomatosis polyposiscoli

• Ifgeneisonlyknownbymutantallele,theallelenameservesasaplaceholdergenename– Leprdb usedtobeDbdb

• Loci:D8Mit17=the17thlocusmappedtomouseChromosome8byM.I.T.

Genotypes

• MRL/MpJ-Faslpr/J– Geneticbackground– Gene– Allele– Labdesignation

Genotypes

http://www.informatics.jax.org/mgihome/nomen/index.shtml

Proteins

• Allcapitalletters• Noitalics

• E.g.LEPRorAPC

Comparison

Species gene proteinC.elegans actn1 ACTN1Drosophila Act5C Act5CYeast ACT1 Act1pZebrafish actb1 Actb1Mouse Acta1 ACTA1Human ACTB ACTB

Sources

http://www.informatics.jax.org/mgihome/nomen/http://jaxmice.jax.org/support/nomenclature/tutorial.htmlhttp://www.informatics.jax.org/mgihome/nomen/gene.shtml

Homework

• Drosophila(Due.Mar.28)• Yeast(DueApr.4)