BioConfirm B.08.00 Presentation

17 June 2016

BioConfirm B.08.00 Customer Presentation

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Major Enhancements to Increase Your ProductivityThe Latest Version of our BioConfirm Product, B.08.00

BioConfirm is protein and peptide characterization software

using accurate mass LC/MS and MS/MS data from Agilent

instruments. Major new features are:

• Usability and user experience improvements

• New disulfide bond mapping and scrambling workflow

17 June 2016

BioConfirm B.08.00 Customer Presentation

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For Research Use Only. Not for use in diagnostic procedures.

BioConfirm B.08.00 Offers the Lab Administrator

BioConfirm B.08.00 Customer Presentation

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BioConfirm B.08.00 out of the box increases productivity of your lab! No need to create multiple methods or to manage sequence

lists. Spend your time only reviewing samples that need your expert attention.

With Automatic mass range detection

• Never miss proteins ortheir modifications orimpurities

• No need to create aspecial method perBiomolecule or samplecondition.

Standalone Sequence Manager

• Organize your sequencesat one central database

• Cached preprocessingresults makes dataanalysis faster, increasesthroughput

Easy Batch view

• Find the sample that needsreview

• Red/ Green / Yellow flags

• Result review only modefor Biologist can restrictthem from changingmethod parameters

17 June 2016For Research Use Only. Not for use in diagnostic procedures.

BioConfirm B.08.00 Offers the Biologist

BioConfirm B.08.00 Customer Presentation

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Tell BioConfirm B.08.00 how you prepared your sample and collect your report! No need to understand Mass Spectrometry!

Answer 4 questions about

your sample

Press the green button

BioConfirm will tell you how

well the protein was confirmed,

along with reports and results

17 June 2016For Research Use Only. Not for use in diagnostic procedures.

Simple and Clean UI for both: Lab Admin and Biologist

BioConfirm B.08.00 Customer Presentation

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BioConfirm B.07.00 and previous versions were add on of MassHunter

Qual.

BioConfirm B.08.00 is a separate application which works side by

side with MassHunterQual.

There is also centralized Sequence Management by Sequence Manager

application.

17 June 2016For Research Use Only. Not for use in diagnostic procedures.

BioConfirm B.08.00 – Disulfide Bond Mapping

17 June 2016

BioConfirm B.08.00 Customer Presentation

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Cysteine disulfide bonds

- assist in maintaining structure of proteins

- incorrect disulfide linkages can generate protein misfolding

- important to identify location of these bonds and confirm that

they are correct

BioConfirm B.08.00 workflow for:

- Disulfide bond mapping

- Disulfide bond scrambling

For Research Use Only. Not for use in diagnostic procedures.

BioConfirm B.08.00 – Disulfide Bond Mapping

17 June 2016

BioConfirm B.08.00 Customer Presentation

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16 disulfide bonds total

- 4 intra chain (light

chain)

- 8 intra chain (heavy

chain)

- 4 inter chain

Sample prep is very

important for this workflow

as it can cause non-native

scrambling of disulfide

bonds

For Research Use Only. Not for use in diagnostic procedures.

BioConfirm B.08.00 – Disulfide Bond Mapping

17 June 2016

BioConfirm B.08.00 Customer Presentation

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For Research Use Only. Not for use in diagnostic procedures.

BioConfirm B.08.00 – Sample Comparison

17 June 2016

BioConfirm B.08.00 Customer Presentation

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For Research Use Only. Not for use in diagnostic procedures.

BioConfirm B.08.00 – Deconvolution Mirror PlotAllows you to compare biosimilars or lot-to-lot variation

17 June 2016

BioConfirm B.08.00 Customer Presentation

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For Research Use Only. Not for use in diagnostic procedures.

Sample-based Navigation

17 June 2016

BioConfirm B.08.00 Customer Presentation

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For Research Use Only. Not for use in diagnostic procedures.

New BioConfirm tab in Acquisition Method Editor

17 June 2016

BioConfirm B.08.00 Customer Presentation

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For Research Use Only. Not for use in diagnostic procedures.

Disulfide Bond Mapping Application NoteP/N 5991-6951EN

17 June 2016

BioConfirm B.08.00 Customer Presentation

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For Research Use Only. Not for use in diagnostic procedures.

ASMS 2016 Poster on Disulfide Bond Mapping

17 June 2016

BioConfirm B.08.00 PS Training June 2016

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For Research Use Only. Not for use in diagnostic procedures.

Summary

Agilent offers a complete workflow for the

characterization of intact proteins and peptide digests:

• Solid, reliable LC/MS hardware

• Dedicated consumables in the AdvanceBio columns line

• Integrated software from data acquisition through reporting

• Open access productivity with MassHunter Walkup that

allows new users to be trained in five minutes

New features have been added to BioConfirm B.08.00

for enhanced usability and disulfide bond mapping

17 June 2016

BioConfirm B.08.00 Customer Presentation

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For Research Use Only. Not for use in diagnostic procedures.

17 June 2016

BioConfirm B.08.00 Customer Presentation

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