Done by:

Roaa Al-shareef .

Supervisor :

Mrs. Hibah Abu-Sulaiman

Last time we were chefs

agar, broth .

This Picasso we are !

culturing, staining.

Culturing techniques.

Condition of incubation.

Reading culture plates.

Preparing bacterial smears.

Staining using Gram Stain.

To draw our pictures colors :

Sample

for Picasso's environment :

Cleaning the paintbrush .

Bring the painting board .

Drowning then painting.

Cleaning the paintbrush.

For laboratory medicine environment :

In order to get an excellent staining and

chemical tests .. colonies should be gotten.

A colony is gotten in a appropriate Petri

dish .

Microorganisms are similar to more complex

organisms in that they need a variety of

materials from their environment to function

and to live, which is :

Suitable Temperature .

Suitable Ph .

Air content .SALINITY.

Pressure .

Organisms are sensitive to changes in temperature because the folding of the proteins and DNA in the cell can be disturbed.

What do you think will happen if you heated protein !!

ANDWhat do you think will happen if you freeze it !!

Both the use of heat and cold allows for

control of bacterial growth by either

destroying the microbe (heat) or

preventing further growth (cold).

According to temperature :

require temperatures

above 80°C .

Again..!

Organisms are sensitive to changes in pH

because the folding of the proteins and

DNA in the cell can be disturbed.

What do you think is the suitable PH for

bacterial growth !

Other types of bacteria :

Microaerephilic:

This organisms live

with low concentration

of oxygen.

Capnophilic:

these organisms must

have an environment rich

in carbon dioxide.

there is certain categorize to read colonies

according to

shape

Size

Texture

color

other features.

The ability to alter or destroy the red blood cells and

create a color change in the medium.

There is :

A .Beta hemlysis :

colonies are surrounded by a clear zone.

B.Alpha hemolysis:

colonies are surrounded by a zone greenish

Color.

C.Gama hemolysis:

No hemolysis.

size :The colonies either big or small,

maybe in between .

margin :

The colonies present a smooth

Margin , filamentous, irregular or

Rough.

Elevation : The colonies rise from

The plate forming an elevations

Color : Colonies present a basic colors:

White , yellow , gray .