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The world leader in serving science EfficientFeed+ Supplements
The world leader in serving science
EfficientFeed+ Supplements
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How would flexibility and simplicity benefit your process development or scale-up?
Enjoy an Improved Process with These Feeding Benefits
Flexibility Simplicity
Reduce feed volume for maximum working volume
Reduce feed volume for less dilution
Increase feeding within the available space for higher titers
Comparable performance when scaling up, switching between liquid and Gibco™ Advanced
Granulation Technology™ (AGT™) formats
Easier reconstitution decreases potential errors
Fewer closed-system connections reduce contamination risk
3
EfficientFeed A+, B+, and C+ Supplements
Available in two formats
1 AGT format • 1, 10, and 100 L
• Can be concentrated
2 Liquid format Available in 1 L bottle and in 10 L BioProcess Container with Thermo Scientific™
Aegis™ 5-14 film
Liquid formats are concentrated
• Gibco™ EfficientFeed™ A+ (3X) Supplement
• Gibco™ EfficientFeed™ B+ (3X) Supplement
• Gibco™ EfficientFeed™ C+ (2X) Supplement
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Flexibility
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Maximize Bioreactor with Concentrated Liquid and EfficientFeed AGT Supplements
71 71 88
2910
1219
0
20
40
60
80
100
120
Traditional Dilution Maximum working volume
Available space Feeding supplement Media working volume
Decrease the need to compromise on feed optimization
Volu
me
(L)
~20% improvement in bioreactor use
3x
3x
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Achieve Greater Productivity with Reduced Product Dilution
Notes: EFC = EfficientFeed C
Tite
r (%
)
Integral viable cell density (%)
Theoretical qp
0
50
100
150
200
250
0 100 200 300 400
Glucose feed only
30% EFC AGT
15% EFC AGT
15% EFC+ 2X
CHO DG44 cells in Gibco™ CD FortiCHO™ medium supplemented with 30% EfficientFeed C or 15% EfficientFeed C+ AGT supplement
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Simplicity
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Comparable Performance Between Liquid and AGT Formats
* Control: average viable cell density of AGT** Control: average titer of AGT
EfficientFeed supplement with CHO-S:average growth
EfficientFeed supplement with CHO-S:average titer
3 5 7 10 12 140
50
100
150
Nor
mal
ized
per
cent
of c
ontro
l*
Time (days)
EFA+ 3X Liq
EFA+ 3X AGT
EFB+ 3X Liq
EFB+ 3X AGT
EFC+ 2X Liq
EFC+ 2X AGT
0
50
100
150
Nor
mal
ized
per
cent
of c
ontro
l**
Time (days)
EFB+ 3X Liq EFC+ 2X Liq
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EFB+ 3X AGT EFC+ 2X AGTEFA+ 3X AGT
EFA+ 3X Liq
9
90
80
70
60
50
40
30
20
10
0
EfficientFeed AGT Supplements Provide Consistent Protein Quality
Consistency in protein quality means optimizing feeding won’t require regulatory trade-offs
Man5 G0F G1F G2F
Rel
ativ
e pe
rcen
tage
30% EFC 30% 1x EFC+ 15% EFC 15% 2x EFC+X X
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More Steps = More Risks
* QS = Quantity sufficient
Risks associated with more steps:EfficientFeed liquid supplement
EfficientFeed AGT supplement
Other single-part feed
16steps
Multiple-part feed
Add water Mix QS*/pH
adjust Mix QS* Mix Filter Hold Add water Mix QS*/pH
adjust Mix QS* Mix Filter Use
10steps
6steps
1step
Add water Mix pH
adjust MixpH
back to neutral
Mix QS* Mix Filter Use
Add water Mix QS* Mix Filter Use
Use • Safety in handling caustics for pH adjustment• Overshooting salt addition in pH adjustment• Reconstitution errors• Local high-pH regions or hotspots
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Room Temperature Simplicity: Reduces Contamination Risk
Fewer closed-system connections = less contamination risk
Daily feeding
Standard bolus feeding
EfficientFeed supplements 1
4
10
Typical number of closed-system connections required
EfficientFeed+
12
Superconcentrated
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Designed for more flexibility and simplicity
EfficientFeed A+, B+, and C+ AGT Supplement Design
Superconcentrated Neutral pH Simple to prepare Hydrates quickly Flexible
Target: 150–200 g/L
pH range: 6.5–7.1
Just add water ≤90 min
Add less water to concentrate
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Superconcentrate AGT Format Easily by Adding Less Water
Less dilution can mean easier downstream processing
Example: comparison of final volumes for reconstitution of10 L packages of EfficientFeed A+, B+, and C+ supplements
0
2
4
6
8
10
12
1X 2X 3X
Volu
me
(L) 10
53.33
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Superconcentration From Original Concentrates of ~50–80 g/L to ~150–200 g/L
EfficientFeed supplement concentrations
Concentration factor
0
50
100
150
200
250
1x 2x 3x
Con
cent
ratio
n (g
/L)
1X 2X 3X
A or
A+
B o
r B+
C o
r C+
A+B+
C+
A+
B+
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No Long Mixing Times Required Even at Highest Concentrations: EfficientFeed A+, B+, and C+ AGT Supplements Are pH-Neutral
EfficientFeed products are easy to reconstitute and use
EfficientFeed A+ EfficientFeed B+ EfficientFeed C+
6.0
6.2
6.4
6.6
6.8
7.0
7.2
7.4
0 50 100 150 200 250
pH
Concentration (g/L)
0
20
40
60
80
100
0 50 100 150 200 250
Reh
ydra
tion
time
(min
)
Concentration (g/L)
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Summary and Ordering Information
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Accomplish more while minimizing risk with EfficientFeed supplements
Catalog EfficientFeed Supplements
Flexibility Simplicity
Reduce feed volume for maximum working volume
Reduce feed volume for less dilution
Increase feeding within the available space for higher titers
Comparable performance when scaling up, switching between liquid and Gibco™ Advanced
Granulation Technology™ (AGT™) formats
Easier reconstitution decreases potential errors
Fewer closed-system connections reduce contamination risk
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When more than one EfficientFeed product may be needed to get the best result, this kit allows you to test 1 L of each to determine the best fit
Gibco Feed Kits Are Available to Simplify Testing
• 3 L of EfficientFeed+ AGT feeding supplements for the price of ~1.5 L
• Contents of kit
– 1 L EfficientFeed A+ Supplement
– 1 L EfficientFeed B+ Supplement
– 1 L EfficientFeed C+ Supplement
– Protocols (manual) on a flash drive
What you get with the kit:
How to order:Gibco™ Feed Kit A+, B+, C+ (Cat. No. A3315801)
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AGT Format Ordering Information
Product Quantity Cat. No.
EfficientFeed A+ AGT Supplement 1 L A2502304
EfficientFeed A+ AGT Supplement 10 L A2502305
EfficientFeed A+ AGT Supplement 100 L A2502301
EfficientFeed B+ AGT Supplement 1 L A2503004
EfficientFeed B+ AGT Supplement 10 L A2503005
EfficientFeed B+ AGT Supplement 100 L A2503001
EfficientFeed C+ AGT Supplement 1 L A2503104
EfficientFeed C+ AGT Supplement 10 L A2503105
EfficientFeed C+ AGT Supplement 100 L A2503101
Gibco Feed Kit A+, B+, C+ 3 L A3315801
thermofisher.com/efficientfeed
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Liquid Format Ordering Information
Product Quantity Cat. No.
EfficientFeed A+ 3X Supplement 1,000 mL A3937401
EfficientFeed A+ 3X Supplement 10 L A3937402
EfficientFeed B+ 3X Supplement 1,000 mL A3937501
EfficientFeed B+ 3X Supplement 10 L A3937502
EfficientFeed C+ 2X Supplement 1,000 mL A3937601
EfficientFeed C+ 2X Supplement 10 L A3937602
thermofisher.com/efficientfeed
Custom orders are now available in any BioProcess Container (BPC) size
The world leader in serving science
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals. © 2019 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. COL19371 0719
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EfficientFeed Supplement and Media Guide
These are not universal, but would be a good place to start
CHO cell type Banking medium contains growth factor
Customer will sequentially adapt cells Media option Matched feed option
CHO-S N Y Gibco™ Dynamis™ Medium EfficientFeed C+ Supplement
Gibco™ ExpiCHO-S™ Y Y Gibco™ ExpiCHO™ Stable Production Medium (with GlutaMAX™ added) EfficientFeed C+ Supplement
ExpiCHO-S N N ExpiCHO SPM EfficientFeed C+ Supplement
DG44/DXB11 N N Gibco™ CD OptiCHO™ Medium EfficientFeed A+ Supplement
CHO-K1 N Y Dynamis Medium EfficientFeed C+ Supplement
GS CHO N Y Dynamis or CD OptiCHO Medium EfficientFeed C+ or B+ Supplement
Other CHO N Y Dynamis Medium EfficientFeed C+ Supplement
