Organizing committee - CNSHfbb.hcmus.edu.vn/vnt_upload/news/12_2016/2017-HCMUS-KU... ·...

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Organizing committee

Chair Man Bock Gu School of Life Sciences and Biotechnology for Brain

Korea21 PLUS, Korea University, Korea

Co-Chair Tri-Nhan Nguyen Faculty of Biology - Biotechnology, University of Science,

Vietnam National University Ho Chi Minh City

Committee

members

In-Geol Choi School of Life Sciences and Biotechnology for Brain


Sung Ok Han School of Life Sciences and Biotechnology for Brain

Korea21 PLUS Korea Universtiy, Korea University, Korea

Hyun Jin Park School of Life Sciences and Biotechnology for Brain


Kwang Won Lee School of Life Sciences and Biotechnology for Brain


Sung Joon Lee School of Life Sciences and Biotechnology for Brain


Van-Thuan Nguyen School of Life Sciences and Biotechnology for Brain

Korea21 PLUS, Korea Universtiy, Korea

Van-Hieu Tran Faculty of Biology - Biotechnology, University of Science,


Duc-Hoang Nguyen Faculty of Biology - Biotechnology, University of Science,


Hai-Nhung Truong Faculty of Biology - Biotechnology, University of Science,


Bao-Ha Tran-Le Faculty of Biology - Biotechnology, University of Science

- VNUHCM

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Symposium Venue

Room F102, University of Science – VNUHCM, 227 Nguyen Van Cu St., Dist. 5, Ho Chi Minh City, Vietnam

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Program at a Glance

Plenary session

8.30 ~ 8.40 Introduction of KU/Department of Biotechnology for BK21 PLUS Prof. Man Bock Gu…………………………………………………………..………… 5

8.40 ~ 8.50 Introduction of HCMUS/ Faculty of Biology - Biotechnology Dr. Nguyen Tri Nhan…………………………………………………………………....5

Session 1. Food Biotechnology and Therapeutics

Chairs: Associate Prof. Nguyen Duc Hoang, Prof. Man Bock Gu

8.50 ~ 9.05

Inorganic Arsenic Bioaccessibility/Bioavailability from Cooked Rice Using

In Vitro Digestion/Caco-2 Cell Model

Kwang Won Lee

Department of Biotechnology, School of Life Sciences and Biotechnology, Korea

University, Seoul, Republic of Korea………………………………………………… 7

9.05 ~ 9.20

Nano delivery system for practical food system

Hyun Jin Park


University, Seoul, Republic of Korea ……………………………………………….. 8

9.20 ~ 9.35

Mechanism of dietary anthocyanins on the regulation of energy metabolism

Sung-Joon Lee

Department of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea ………………………………………………. 10

9.35 ~ 9.50

Mesenchymal stem cell: a potential therapy for liver cirrhosis treatment

Nhung Hai Truong

Faculty of Biology - Biotechnology, HCM University of Science………………….12

9.50 ~ 10.05

Nano chitosan for protein delivery

Hieu Tran-Van

Faculty of Biology - Biotechnology, HCM University of Science………………….13

10.05 ~ 10.15 Coffee break

Session 2. Biosensor and Bioengineering

Chairs: Dr. Nguyen Tri Nhan, Prof. In-Geol Choi

10.15 ~ 10.30

C1/3/5/6 Biorefinery by using Designer Nanoscale Enzyme Complexes

Sung Ok Han

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University, Seoul, Republic of Korea………………………………………………. 15

10.30 ~ 10.45

Aptamer-based Nanobiosensors and Biomolecular Technology Research at Korea University

Man Bock Gu


University, Seoul, Republic of Korea ………………………………………………. 17

10.45 ~ 11.00

Computational & Synthetic Biology Laboratory - the Future of G3 Technology In-Geol Choi


University, Seoul, Republic of Korea………………………………………………. 18

11.00 ~ 11.15

Acellular adipose matrix: collection, evaluation, potential applications

Tran Le Bao Ha

University of Science, Vietnam National University, Vietnam ……………………19

11.15 ~ 11.30 Non-inducible and IPTG-inducible expression vectors for protein production based on Pgrac promoters for Bacillus subtilis

Nguyen Duc Hoang

Faculty of Biology - Biotechnology, VNUHCM-University of Science, Hochiminh City, Vietnam…………………………………………………………………….…….20

11.30 ~ 11.40 Photo & Coffee break

11.40 ~12.15

Q&A for students & Interview-Recruiting student session

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Plenary session

8.30 ~ 8.40

Introduction of KU/Department of Biotechnology for BK21 PLUS

Prof. Man Bock Gu

8.40 ~ 8.50

Introduction of HCMUS/ Faculty of Biology - Biotechnology

Dr. Nguyen Tri Nhan

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Session 1. Food Biotechnology and Therapeutics

Chairs: Dr. Nguyen Tri Nhan (Faculty of Biology - Biotechnology, HCM University of Science)

Prof. Man Bock Gu (Department of Biotechnology, School of Life Sciences and Biotechnology,

Korea University, Seoul, Republic of Korea)

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Abstract Session 1. Food Biotechnology and Therapeutics

Inorganic Arsenic Bioaccessibility/Bioavailability from Cooked Rice

Using In Vitro Digestion/Caco-2 Cell Model

Kwang Won Lee

Department of Biotechnology, School of Life Sciences and Biotechnology,

Korea University, Seoul, Republic of Korea

E-mail: [email protected]

Rice is one of the major sources for exposing inorganic arsenic (As) in food. Using in

vitro digestion/Caco-2 cell model, the bioaccessibility and bioavailability of As from

cooked rice were studied. Inorganic arsenic, especially As (III), was predominant and

bioaccessible species in cooked rice. The percentage of bioaccessibility of total As in

white rice (75%) was slightly higher (p=0.061) than brown rice (66%). However, there was

no different of bioaccessibility of iAs between white rice (95%) and brown rice (96%). In

Caco-2 cell monolayer, As uptake considering sum of retention and transport was

determined to be 16 to 38 %. As bioavailabilities in cooked white and brown rice were

31 % and 21 %, respectively indicating that the bioavailability of As from cooked rice can

be affected by matrix environment. The in vitro digestion/Caco-2 cell model may provide

an important information on risk assessment of As in rice.

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Nano delivery system for practical food system

Hyun Jin Park




Nutraceuticals/functional foods can prevent various diseases and enhance physiological

performances. However, there are some limitations due to their poor solubility and very

low bioavailability. It is necessary to find out the optimum nanoformulations for different

nutraceuticals to improve their water solubility, absorption rate, and bioavailability. The

aim of this study is to provide a potential of nano delivery systems such as nanostructured

lipid carriers(NLCs), nanoemulsions(NEs), and nanoliposomes(NLs) which could improve

the encapsulation efficiency and bioavailability.

Chitosan coated NLCs(CH-NLCs) were developed for iron fortification by melt dispersion

method. Physicochemical properties of NLCs and CH-NLCs were investigated by

different concentration of emulsifier and chitosan to determine the optimum condition of

NLCs and CH-NLCs. In case of capsaicin NEs, the solubility of capsaicin in different oils

was measured to find out optimum NE formulations. NEs were produced on various ratios

of oil, surfactant, and water. Skin permeation studies were performed using a franz

diffusion cell method. Confocal laser scanning microscopy images were examined to

verify the penetration of the capsaicin NE. Chitosan coated curcumin nanoliposomes(CS-

Cur-NLs) were fabricated by ethanol injection method using cholesterol and L-α-

phosphatidylcholine (EPC) in ratio of 1 to 2. The mucoadhesive property of CS Cur-NLs

was measured by mucin adsorption study. The release rate of nano-carriers was tested

and their morphologies were characterized using transmission electron microscopy.

In conclusion, the encapsulation efficiency and in vitro release test showed that CH-

NLCs had a great potential for use of iron fortification in milk. In case of capsaicin NEs, it

was successfully developed by controlling the ratios of the surfactant mixtures, the oil

phase, and aqueous phase to enhanced skin permeation. For CS-Cur-NLs, the

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mucoadhesive property of CS-Cur-NLs was higher than that of non-coated Cur-NLs. CS-

Cur-NLs will show a potential for the prolonged adsorption in the gastrointestinal tract due

to higher mucoadhesion.

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Mechanism of dietary anthocyanins on the regulation of energy

metabolism

Sung-Joon Lee




Cyanidin is a major dietary anthocyanidin in several plant-based foods including berries

and colored rice. It has been suggested that cyanidin has potent biological activities in

the regulation of cellular energy metabolism; however, molecular target(s) and the mode

of action of cyanidin have been elusive. The surface plasmon resonance and time-

resolved fluorescence resonance energy transfer analyses revealed that cyanidin and its

glycosides interacted with nuclear hormone receptorsk peroxisome proliferator-activated

receptors (PPARs) and liver X receptors, but with the highest affinity to PPARα. Cyanidin

and its glycoside significantly reduced triglyceride concentration in lipid-loaded HepG2

cells, via activation of the rate of fatty acid oxidation and suppression of the rate of fatty

acid synthesis in cultured hepatocytes. In high-fat-diet fed mice, oral administration of

cyanidin glycoside reduced plasma and hepatic triglyceride concentrations and improved

glucose and insulin tolerance, however, these metabolic activities of cyanidin glycoside

were abrogated in PPARα deficient mice. These suggest that PPARα may be a major

protein target for cyanidin and its glycoside in vivo. Several line of research showed that

cyanidin also activated AMP-activated kinase, which is a key regulator in cellular energy

metabolism and our recent results revealed that cyanidin glycoside activated AMP-

activated kinase not by direct allosteric mechanism but by indirect stimulation of upstream

kinase. Collectively, these results demonstrate that cyanidin may interact with more than

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one protein regulator, potentially with the highest affinity to PPARα. Activation of liver X

receptor and AMP-activated kinase as well as PPARα may and liver X receptor and

stimulate AMP-activate kinase may together contribute to the reduction of hepatic and

plasma lipid accumulation and improvement of insulin sensitivity.

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Mesenchymal stem cell: a potential therapy for liver cirrhosis treatment

Nhung Hai Truong1,2*, Nam Hai Nguyen1, Trinh Van Le1, Huy Minh Le3, Nghia Huynh3,

DatQuoc Ngo3, Thanh Van Nguyen4, NgocKim Phan1,2, Phuc Van Pham1,2

1Laboratory of Stem Cell Research and Application, University of Science, VNU-HCM,

Vietnam

2Faculty of Biology and Biotechnology, University of Science, VNU-HCM, Vietnam

3University of Medicine and Pharmacy, HCM city, Vietnam

4Nguyen Tat Thanh University, Ho Chi Minh city, Vietnam

*Corresponding author email: [email protected]

Livercirrhosis is defined as the replacement of healthy liver tissues by fibrosis and regenerative

nodule formation. Cirrhosis ranks 14th in the world and 4th in Central Europe as cause of death.

Orthotopic liver transplantation (OLT) is the priority treatment for decompensated livercirrhosis.

Nonetheless, patients face many obstacles, such as the high costs, high risk from invasive

surgery, limitation of donor tissue, and lifelong immunosuppressive treatment. In recent years,

stem cell therapy has become a promising therapy for treatment. Some in vitro studies and in

vivo preclinical trials have demonstrated that mesenchymal stem cells (MSCs) may improve

livercirrhosis. However, application of MSCs in livercirrhosis treatment is still controversial

issues including MSC sources, routes of transplantation, “homing” into liver and hepatic

differentiation in vivo. To clarify any ambiguity some issues, we test the hypothesis that

autologous and allogenic mesenchymal stem cell sameliorate livercirrhosis in mice. The results

showed that MSC transplantation improved AST/ALT/bilirubin/albumin index after 7 days of

injection (p<0.05); significantly down-regulate gene expression of TGF-beta, procollagen and

nt5e (p<0.05). Both MSCs autologus and allogenic transplantation accelerated liver

regeneration capacity in mice after 21 days of transplantation. Considering safety, MSC

transfusion via a peripheral vein is a potential method for liver fibrosis treatment.

Keywords: Mesenchymal stem cell, livercirrhosis/fibrosis treatment, stem cell therapy, liver

disease, transplantation.

mailto:[email protected]

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Nano chitosan for protein delivery

Hieu Tran-Van

Department of Molecular and Environmental Biotechnology, University of Science,

VNU-HCM

*E-mail: [email protected]

Fibroblast growth factor 2 (FGF-2) is a multi-functional protein regulating many biological

processes, especially those involved in wound healing. However, FGF-2 has short half-

life and is easily degradable in vivo, which limited its use for wound treatment. In this

study, we investigated a drug-delivery model for FGF-2 via incorporation with

biodegradable carboxylmethyl chitosan (CMCS), a substance classified as safe for use

in human by the Food and Drug Administration. We showed that CMCS nanoparticles

(NPs) could be synthesized by ionic gelation method using CaCl2 as a crosslinking

reagent with the CMCS:CaCl2 ratio of 1:0.8. Synthesized CMCS NPs were spherical with

an average diameter of 32.68±6.83 nm, and were non-toxic at concentrations up to 2.5

mg/ml. Incorporated CMCS:FGF-2 NPs had an average diameter of 34.83±5.89 nm and

FGF-2 incorporation efficiency was 95%. CMCS:FGF-2 NPs released 36.36% and 58.47%

of FGF-2 after 48 hours incubation in two different pH of 7.4 and 5.8, respectively. The

incorporation and release processes did not have a significant effect on FGF-2 activity.

Simultaneously, CMCS:FGF-2 NPs could protect FGF-2 from the degradation of trypsin

in vitro. Our results laid the groundwork for the manufacturing of protein incorporated

CMCS NPs for bio-applications.

Keywords: FGF-2, carboxylmethyl chitosan NPs, ionic gelation, drug release

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Session 2. Biosensor and Bioengineering

Chairs: Dr. Nguyen Tri Nhan (Faculty of Biology - Biotechnology, HCM University of Science)

Prof. In-Geol Choi (Department of Biotechnology, School of Life Sciences and Biotechnology,

Korea University, Seoul, Republic of Korea)

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Abstract Session 2. Biosensor and Bioengineering

C1/3/5/6 Biorefinery by using Designer Nanoscale Enzyme Complexes

Sung Ok Han




Cellulolytic anaerobic bacteria have evolved intricate multi-enzyme complexes with

scaffolding proteins known as cellulosomes. Cellulosomes are promising approach for

solving of the problems related to slow enzymatic degradation rates because complexed

system showed degradative potential. Cellulosome system have been applied to improve

the performance of immobilized enzymes and architectural scaffolds have applied to

useful strategies for protein engineering in several industrial and research fields. The

utilization of scaffolds for enzyme immobilization involves advanced bionanotechnology

applications in C1/5/6 biorefinery, which can be achieved by optimizing the function of

various enzymes. The designer enzymes and microbes are a key biological technology

that can be used for C5/C6 biorefinery. Prior to fermentative production of valuable

product from the C5/C6 based polysaccharides such as lignocellulosic and marine

biomasses by microbes, the polymeric substrates are hydrolyzed to release monomeric

sugars. The assembly of minicellulosomes by S. cerevisiae and Corynebacterium

glutamicum increased the activity against various lignocellulosic materials by

approximately 3-fold compared with control. Also, red algae-degrading complexes was

produced by Escherichia coli and increased the activity against the marine biomass

substrate by approximately 2-fold, compared with that for the corresponding enzymes

alone. Furthermore, cellulosome based enzyme complex is proposed for C1 biorefinery.

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C1 gas was successfully converted by functional complexes containing conversion

enzyme and gas binding protein with enhanced gas binding affinity. An enzyme complex

for biological conversion of C1 gas was anchored on the cell surface of industrial strains

and showed 3.3-fold increased conversion efficiency. The assembly of multi-functional

protein complexes showed efficient enzymatic processes by enhancing hydrolysis as well

as interactions between enzymes and their substrates. These results suggest that the

complexed system may be a promising strategy for C1/5/6 biorefinery as biological tools

for production of biochemicals such as bioplastics. Thus, the development of various

configurations of multi-functional protein complexes for use as tools in whole-cell

biocatalyst systems has drawn considerable attention as an attractive strategy for

bioprocess applications.

References

1. J. E. Hyeon, S. D. Jeon, S. O. Han, Biotechnol. Adv., 31(6), 936 (2013)

2. J. E. Hyeon, S. W. Kim, C. Park, S. O. Han, Chem. Comm., 51, 10202 (2015)

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Aptamer-based Nanobiosensors and Biomolecular Technology

Research at Korea University

Man Bock Gu

Department of Biotechnology, Korea University


Aptamers are single-stranded nucleic acids having molecular recognition properties

similar to antibodies, and isolated by in vitro selection and amplification process, SELEX.

This nucleic acid aptamer is well-known with its stable feature, quick and easy

developement, cost-effective production in vitro, and so on. To find a most effective

atpamer screening method has always been a goal, especially for obtaining a pair of

aptamers binding at different sites of a single target. This talk will start with how the

aptamers are innovatively screened, for the first time in the world, by using a new nano-

material, graphene, without the immobilization of targets. A few of successful examples

using immobilization-free screening of aptamers will be presented, including a few

aptamer duo cases, inevitable for being applied in a stripe-type platform, which could be

a reliable platform for commercialization eventually. In addition, some examples obtained

from the interactions among biomolecules, nanoparticles, or targets will be shown for

aptamers, bioluminescent bacteria, and enzymes with their successful implementation.

In addition, the benefits of using nano-sized materials for biosensing and other

biotechnological applications will be presented with scientifically proven clear examples

of different organic-inorganic hybrid forms of nanomaterials and biomolecules.

Keywords: Aptamers, Graphene-oxide, Enzyme stabilization, Bioluminescent Bacteria


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Computational & Synthetic Biology Laboratory - the Future of G3

Technology

In-Geol Choi




Biology is Technology. The Computational and Synthetic Biology Laboratory (CSBL)

aims to discover biological knowledge and make a full integration of theory and practice.

We construct metabolic pathways in genomes by design (e.g. iPNN - intelligent Pathway

Network Navigator) and learn how nature constructs ‘things’ by synthesis (e.g. PKSDS -

PolyKetide Synthetase Design Suite). CSBL gears undergraduate research programs,

the Korea_U_Seoul team for iGEM and supports DIYBio Movements in Korea. One of the

major achievements made by the CSBL is elucidation of the details of bacterial agarolytic

pathways. Agar, a recalcitrant polysaccharide, has a great potential as a renewable

biomass. We have sequenced genomes (DNAseq) and transcriptomes (RNAseq) of

several agarolytic microorganisms using next generation sequencing (NGS) techniques.

We have identified key enzymes (e.g. beta-agarases, agarooligosaccharide beta-

galactosidase - ABG, neoagarobiose hydrolase - NABH, anhydrogalactose

dehydrogenase -AHGD and anhydrogalactonate cycloisomerase - ACI, etc.) in the agar

metabolic pathway and determined atomic structures of key enzymes. The full

understanding molecular and cellular functions of these novel agarolytic enzymes will

provide the design principle of synthetic agar degradation pathways and eventually guide

the construction of synthetic microorganisms converting agar into valuable chemicals.

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Acellular adipose matrix: collection, evaluation,

potential applications

Tran Le Bao Ha1*, Nguyen Thi Ngoc My1, Nguyen Duc Thai1, Michele Zocchi2

1University of Science, Vietnam National University, Vietnam

2Turin Medical University, Italy


Our research interest is tissue engineering which includes fabrication of new biomaterials

as scaffolds for cells/stem cells into generation of functional tissues. Currently, we focus

on soft and hard tissue engineering as well as cancer tissue engineering. One of

concerned biomaterials is acellular adipose matrix (AAM) which is used not only for tissue

engineering as a natural scaffold, but also for fat grafting. Our group has started to

establish a reliable and efficient method for producing AAM. AAM is obtained from

liposuction adipose tissue and prepared by a decellularization process to eliminate all the

cellular components and reducing the DNA content to minimal levels. We also aim to

examine AAM as a scaffold for soft-tissue regeneration. In the near future, we aim to

produce AAM as ready-to-use, off the shelves and suitable materials for many clinical

applications.

Key words: tissue engineering, biomaterial, decellularization, scaffold, regeneration

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Non-inducible and IPTG-inducible expression vectors for protein

production based on Pgrac promoters for Bacillus subtilis

Trang Phan, Nguyen Duc Hoang*

Faculty of Biology - Biotechnology, VNUHCM-University of Science, Hochiminh City,

Vietnam; *E-mail: [email protected]

Bacillus subtilis possesses excellent properties to be considered as a host for protein

production. These properties include GRAS status and available large scale production.

Also, this endotoxin-free bacterium confers significant advantages to producing

recombinant protein products for animal and human uses. The development of these

products requires efficient optional expression systems for this bacterium. Here, we will

present a topic on the development of pHT expression vectors based on the promoter

Pgrac family to produce recombinant proteins in B. subtilis, which can use in an IPTG-

inducible or non-inducible manners. First, we introduced a new synthetic promoter Pgrac

derived from B. subtilis groESL promoter and E. coli lac operator, which can be induced

by addition of IPTG. This promoter is 50 times stronger than Pspac, the most popular

IPTG-inducible promoter used for B. subtilis. Second, by changing the core promoter

elements and the mRNA stabilizing elements to make an 84-promoter library, we showed

that the second generation of Pgrac promoters could enhance production levels more

than 30% of total cellular proteins in B. subtilis. Third, we generated potent expression

vectors containing a second generation promoter of Pgrac family, which could be used to

produce efficiently intracellular or extracellular recombinant proteins in B. subtilis. Finally,

we will update recent development of novel non-inducible vectors using the IPTG-Pgrac

promoter, which allowed production of recombinant proteins without the addition of IPTG.

The use of IPTG-inducible and non-inducible expression vectors harboring Pgrac

promoter will promise to bring enormous benefits to industrial applications.

Keywords: Pgrac, pHT vectors, Pgrac100, non-inducible vector, Bacillus subtilis.


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Q&A for students and Interview outstanding student session

11.40 ~ 12.15

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