PROJECT LIFE12 ENV/IT/00036 “Anti-infective environmental friendly molecules against plant...

PROJECT LIFE12 ENV/IT/00036

“Anti-infective environmental friendly molecules against plant pathogenic

bacteria for reducing Cu”“AFTER Cu”

Start: 01/01/2014End: 31/12/2015

11/06/201518 Month and monitoring meeting

Firenze - ItalyDISPAA premises


AGENDA18 MONTH AND MONITORING MEETING

• 9:30 [DISPAA] Welcome and opening of the meeting

TECHNICAL MEETING• 09:40 [All] Actions carried out by each

partner and scheduled activities from January 2015 until the end of May 2015.

• 11:15 [All] Coffee break• 11:30 [All] After-Cu Technical future issues: [DISPAA] Technical activities scheduled in

the next six months of the project [All] Open discussion on actions to be

carried out in the next six months of the project

•


AGENDA12 MONTH MEETING

• 12:00 [DISPAA] Management and

dissemination issues, in particular:o [DISPAA] Dissemination activitieso [DISPAA] Preparation of Final Report

• 13:00 Lunch

ADMINISTRATIVE MEETING• 14:30 [EC Monitor team] After-Cu

administrative documents monitor• 16:30 Tour of DISPAA laboratories• 17:00 End of monitoring meeting •


After-Cu PROJECT

GENERAL SUMMARY •




Technical Actions carried out by each partner in the last six months

B.2 (April 2014-March 2015): Demonstration of the qualitative and quantitative yields of conventional chemical synthesis and biotechnological synthesis of the anti-virulence peptides at laboratory scale

B.3 (April 2014-March 2015): Demonstration of the chemical and biological stability of anti-virulence peptides produced by conventional chemical and biotechnological synthesis

B.4 (July 2014-June 2015): Demonstration of Kilo-scale biotechnological synthesis of the anti-virulence peptides by recombinant technology

B.5 (July 2014-June 2015): Demonstration of the null toxicity profile of the anti-virulence peptides on model organisms and microorganisms

B.6 (October 2014-December 2015): Demonstration of the anti-virulence peptides in vivo performances at pilot scale level in field screenings

B.7 (October 2014-December 2015): Demonstration of the effectiveness of the anti-virulence peptides in the presence of contamination by copper compounds at pilot scale level in field screenings

.


Technical Actions carried out by each partner in the last six months

C.2 (April 2014-March 2015): Monitoring of the in vitro anti-virulence performances of peptides at laboratory level

C.3 (April 2014-March 2015): Monitoring of the absence of side effects for the anti-virulence peptides on common targets of any living organism at laboratory level

C.4 (July 2014-June 2015): Monitoring of the absence of a direct selection operated by the anti-virulence peptides towards the emergence of bacteria resistant to the anti-virulence peptides themselves at laboratory level

C.5 (October 2014-December 2015): Monitoring of the short term environmental benefits from the use of the anti-virulence peptides in plant disease control at pilot scale level in field screenings

C.6 (October 2014-December 2015): Monitoring of the absence of an indirect selection operated by the anti-virulence peptides on copper compound and antibiotic resistant bacteria at pilot scale level in field screenings

C.7 (July 2015-December 2015): Monitoring of technical-socio-economic assessment of the After-Cu project

.


Presentations of:

DISPAA CEBAS

ICCOMCNR ASTRA

SOLDANO

.


[All] Open discussion on actions carried out .


Dissemination actions

D1 (July 2015-December 2015): Demonstration workshop in ItalyD2 (July 2015-December 2015): Demonstration workshop in SpainD3 (January 2014-March 2014): Project websiteD4 (January 2014-March 2014): Notice boardsD5 (September 2015-December 2015): Layman's reportD6 (January 2014-December 2015): Diffusion material preparationD7 (January 2014-December 2015): Articles and press releasesD8 (January 2014-December 2015): NetworkingD9 (April 2015-December 2015): After-Cu technology manualD10 (January 2014-December 2015): Demonstration workshop, seminars, conferences and other eventsD11 (January 2014-December 2015): Dissemination to Institutions and policy makersD12 (September 2015-December 2015): After-LIFE Communication Plan D13 (January 2014-December 2015): International fairs and other eventsD14 (January 2014-December 2015): Digital supports for international diffusion



Dissemination actions carried out by each partner in the last six months

D1 Demonstration workshop in Italy (November 2015)

• ASTRA will organise a demonstration workshop in November 2015



D2 Demonstration workshop in Spain (November 2015)

• CEBASCSIC will organise a demonstration workshop in November 2015



D3 Project website 01/02/2014 and continuous update until 31/12/2015

• After-Cu website in English, Spanish and Italian: www.lifeaftercu.com

• In the website: Visit counter Link to LIFE+ Link to each beneficiary website Results update News update Coming up Reserved area Link with the Facebook page for event

booking/registration • Facebook page

http://www.lifeaftercu.com/



D6 Diffusion material preparation 01/02/2014 and continuous update until 31/12/2015

• 6,000 After-Cu brochures• 3,000 After-Cu plastic leaflets• 7 After-Cu tissue posters in Italian and English• 3 Malaga event posters• 1 After-Cu roll-up• 3 project presentations (English, Italian and Spanish) • 500 After-Cu copybooks• 800 After-Cu pens• 200 After-Cu cups• 500 After-Cu pendrives



D7 Articles and press releases 10/01/2014 and continuous update until 31/12/2015

• 6 articles directly related to After-Cu: AGI UNIFI website TUTTOGREEN (insert of “La Stampa” newspaper) Fresh Plaza CHIMET Ambiente BIO

• 4 indirect articles related to the participation of After-Cu to the CNR event (in local and web newspaper)

• 12 indirect articles related to the participation of After-Cu to the ASTRA and ENEA event on 22 January 2015 in local and web newspaper

• 3 articles (Italian, Chinese and English) in Platinum journal



D8 Networking 25/03/2014 and continuous update until 31/12/2015

• Cluster with PODEBA, BIOREM, SOREME, BIONAD, GREEN SITE, SOILPRO, RESAFE, SEKRET, CLEANSED, AIS LIFE, EVERGREEN, HORTISED (LIFE 10-11-12-13-14 projects)

• Specific meetings with photos with BIOREM and CLEANSED

• Networking contacts at the CNR LIFE event at Pisa on 25th March 2014

• Networking contacts at the UNIFI LIFE event at Firenze on 24th October 2014

• Networking contacts at the ASTRA LIFE event at Faenza on 22th January 2015



D10 Demonstration workshop, seminars, conferences and other events 25/03/2014 and continuous update until 31/12/2015

• LIFE CNR event in CNR premises on 25 March 2014• University of Siena event about life, Siena 29 May 2014• Seminar in Facultad de Ciencias - Área de Genética of Universidad de Málaga,

Malaga 14 July 2014• Scuola Nazionale Metodologie Analitiche e Bioanalitiche in Spettrometria di

Massa, Parma, Campus Universitario, Parma- Italy, 26-30 May 2014 • Corso avanzato sull'accoppiamento della spettrometria di massa con tecniche di

separazione in fase liquida, Lucca, 23-26 June 2014 • Erice course on Biophysics of channels and transporters, Erice 11-17 May 2014• Event in EEZ of CSIC in Granada (Spain) on 10 July 2014• BRIGTH 2014 AREA CNR September 26th 2014• UNIFI LIFE event at Firenze on 24th October 2014• Italy-Kazakhstan collaboration project event in Kazakhstan on 14/11/2014• ASTRA LIFE event at Faenza on 22th January 2015• Italy-Kazakhstan day event in Florence on 12/05/2015• Reggio Calabria LIFE workshop (10 July 2015)



D11 Dissemination to Institutions and policy makers 25/03/2014 and continuous update until 31/12/2015

• All the After-Cu beneficiaries are responsible for the organisation of specific communication activities in order to disseminate the project actions and results to Institutions and policy makers.

• The following preliminary contacts with institutions have been taken: o Councillor for the Environment of Pisa City - Italy o Environmental Department of Pisa Area - Italy o Environmental Department of Murcia Area - Spain o Senator of the Italian Government - Italy o Assessorato Agricoltura Regione Emilia-Romagna – Italyo ENEA Manager

• Italy workshop (November 2015)• Spain workshop (November 2015)• Reggio Calabria LIFE workshop (10 July 2015)



D13 International fairs and other events 28/07/2014 and continuous update until 31/12/2015

• International Congress on Phytoremediation of Polluted Soils. Vigo, July 28 – 29, 2014

• 14th International Conference, Na,K-ATPase and related transport ATPases: Structure, mechanism, cell biology, health and disease. 30 August - 5 September 2014, The Netherlands

• XX National Congress Italian Society for Plant Pathology (SIPaV) Pisa, 22-23-24 Settembre 2014 "Environmentally loyal plant protection: from nano- to field-scale“

• Conference Psyringae 2015, Málaga, 2-5 June 2015



D14 Digital supports for international diffusion 01/09/2015 and continuous update until 31/12/2015

• Each beneficiary started to define the specific video of each beneficiary activity

• DISPAA is starting to have some contacts with some expert video companies in order to organise the general structure of the video

• DISPAA is starting to work on the video text


ACTION E.1: Project management ACTION E.2: Monitoring

DISPAA has continuous contact with all project partners for monitoring project activities

Before the month end DISPAA receives from each beneficiary technical inputs for the monitor month summary

At the end of each month DISPAA prepares and sends a summary of the project activities carried out to monitoring team at the end of each month

Definition of management structures Definition of payment procedures of each beneficiary DISPAA organised the kick-off and progress meetings in

scheduled time


ACTION E.1: Project management ACTION E.2: Monitoring

DISPAA defined and sent the Partnership Agreement (following the EC scheme) and collected all the beneficiaries signatures

DISPAA uses project progress indicators for monitoring project activities

DISPAA has revised the project progress indicators in each of the projects coordination meeting in order to check any irregularities

DISPAA is checking the number and type of people reached by each dissemination activity in order to check if the diffusion actions have met their goals


ACTION E.1: Project management

Definition of management structures

Management and technical committe• DISPAA: Stefania Tegli• ASTRA: Vanni Tisselli• SOLDANO: Maurizio Soldano• CEBASCSIC: Carlos Izquierdo• ICCOMCNR: Emilia Bramanti

Administrative committe• DISPAA: Silvia Borselli• ASTRA: Elisabetta Baldassarri• SOLDANO: Domenica Timpano• CEBASCSIC: Maria Teresa Hernandez • ICCOMCNR: Manuela Cempini



Definition of payment procedures of each beneficiary

Each beneficiary has a specific payment responsible• DISPAA: Stefania Tegli selects the project cost formally approved

by department director or DISPAA council• ASTRA: Vanni Tisselli as director selects and decides till an amount

of € 20.000. If the cost are bigger than 20.000€ the decisions are assumed in accordance with Legal Representative.

• SOLDANO: Maurizio Soldano as manager selects and decides • CEBASCSIC: Carlos Izquierdo selects the project cost formally

approved by department director• ICCOMCNR: Emilia Bramanti selects the project cost formally

approved by department director



Definition of project accounting system and cost center

All beneficiaries have defined the following internal specific code (codice commessa) which identify the project and all costs and income related to the project:

• DISPAA: B18C13001830006• ASTRA: X02R14 AFTER-CU LIFE12• CEBAS: 201920• ICCOMCNR: B18C13001830006• SOLDANO: After-CU

For DISPAA, CEBAS and ICCOMCNR VAT is a cost.



Definition of project accounting system and cost center

All beneficiaries respects the procedure of the best value for money for selecting all the project external and consumable costs:

DISPAA, CEBAS and ICCOMCNR: public tender through MEPA ASTRA and SOLDANO: historical supplier or market analysis

All the beneficiaries approved only the costs: directly linked to, and necessary for, carrying out the After-Cu

project; reasonable, justified and comply with the principles of sound

financial management, in particular in terms of economy and efficiency;

compliant with applicable tax and social legislation; and actually incurred during the lifetime of the project, as defined in

the grant agreement, and which could be identifiable and verifiable


After-Cu Technical future issues for the next six months

B.4 (July 2014-June 2015): Demonstration of Kilo-scale biotechnological synthesis of the anti-virulence peptides by recombinant technology

B.5 (July 2014-June 2015): Demonstration of the null toxicity profile of the anti-virulence peptides on model organisms and microorganisms

B.6 (October 2014-December 2015): Demonstration of the anti-virulence peptides in vivo performances at pilot scale level in field screenings

B.7 (October 2014-December 2015): Demonstration of the effectiveness of the anti-virulence peptides in the presence of contamination by copper compounds at pilot scale level in field screenings



C.4 (July 2014-June 2015): Monitoring of the absence of a direct selection operated by the anti-virulence peptides towards the emergence of bacteria resistant to the anti-virulence peptides themselves at laboratory level

C.5 (October 2014-December 2015): Monitoring of the short term environmental benefits from the use of the anti-virulence peptides in plant disease control at pilot scale level in field screenings

C.6 (October 2014-December 2015): Monitoring of the absence of an indirect selection operated by the anti-virulence peptides on copper compound and antibiotic resistant bacteria at pilot scale level in field screenings

C.7 (July 2015-December 2015): Monitoring of technical-socio-economic assessment of the After-Cu project



[All] Open discussion on actions to be carried out in the next six months of the project


After-Cu Dissemination future issues for the next six months

D1 Workshop in Italy: OK November 2015 D2 Workshop in Spain: OK November 2015 D3 Project website: update D6 Diffusion material preparation: OK (foreseen 25 Posters, 10,000

leaflets/brochures/factsheets, 2,500 various branded items, now 9,000 brochures/leaflets, 10 posters, 1 roll-up, 3 presentations, 2,000 gadgets)

D7 Press and media releases: OK (foreseen 30 articles, now 25) D8 Networking: OK OK (foreseen 10 projects, now 12) D9 After-Cu technology manual: to be started (foreseen 1 manual) D10 Demonstration workshop, seminars, conferences and other events:

OK OK (foreseen 4 events, now 13 events) D11 Dissemination to Institutions and policy makers: OK OK (foreseen

successful communication, now 10 policy makers meetings) D12 After-LIFE Communication Plan: to be started (foreseen 1 report) D13 International fairs and other events: OK OK (foreseen 4 fairs, now 4

events) D14 Digital supports for international diffusion: start (foreseen project

video)


ADMINISTRATIVE ISSUES

Final Report 31/03/2016 Next Deliverables Partner costs May 2015 Daily rate analysis Next meeting


FINAL REPORT

DISPAA will be responsible of the production of the Final report which will be made up by:

Progress report with the summary of technical, administrative and dissemination project issues

Dissemination Annex Deliverables Output indicators Financial forms with all the originals signatures from each partner Audit document

The EC ok of the Final report will produce the project final payment from the EC


FINAL REPORT

Documents needed from each beneficiary

DISPAA will check all the partner financial documents for final

original versions with signatures from each partner

DISPAA will be responsible for the production of an audit document

signed by an external authorised auditor (already selected) which

will certify all the project financial data.

The selected auditor will check all the partner financial data and will be

free to request to each partner more detailed documents.


NEXT DELIVERABLES

All the following Deliverables will be annexes of the Final Report : Demonstration of the kilo-scale biotechnological synthesis of the anti-

virulence peptides by recombinant technology: Action B.4 Demonstration of the anti-virulence peptides in vivo performances at

pilot scale level in field screenings: Action B.6 Demonstration of the effectiveness of the anti-virulence peptides in the

presence of contamination by copper compounds at pilot scale level in field screenings: Action B.7

Monitoring of the absence of a direct selection operated by the anti-virulence peptides towards the emergence of bacteria resistant to the anti-virulence peptides themselves at laboratory level: Action C.4

Monitoring of the short term environmental benefits from the use of the anti-virulence peptides in plant disease control at pilot scale level in field screenings: Action C.5


NEXT DELIVERABLES

All the following Deliverables will be annexes of the Final Report : Monitoring of the absence of an indirect selection operated by the anti-

virulence peptides on copper compound and antibiotic resistant bacteria at pilot scale level in field screenings: Action C.6

Monitoring of technical-economic viability of the After-Cu project: Action C.7

After-Cu technology manual: Action D.9 After-LIFE Communication Plan: Action D.12 Layman’s Report: Action D.5


Partner costs May 2015

PARTNER CLAIM

PersonnelPersonnel May 2015 Travel

Travel May 2015

External assistanc

e

External assistance May 2015

Consumables

Consumables May 2015

Other costs

Other costs

May 2015Overhea

ds

Overheads May 2015 TOTAL

TOTAL May 2015

DISPAA 205.000 185.550,10 12.500 3.688,14 88.300 41.161,48 105.000 32.862,94 - 30.000 18.428,39 440.800 281.691,05

ASTRA 137.500 70.091,92 12.500 2.155,55 27.800 16.650,00 45.000 5.675,38 - 247,64 16.000 6.637,43 238.800 101.457,92

Soldano 101.500 20.963,32 12.500 22.800 15.000,00 45.000 9.584,18 - 11.930 3.188,33 193.730 48.735,83

ICCOMCNR 52.000 44.262,94 12.500 1.035,04 13.200 9.999,97 25.000 12.090,49 - 7.000 4.717,19 109.700 72.105,63

CEBAS 155.000 74.090,80 12.500 6.484,86 18.400 4.097,76 95.000 66.393,29 - 19.000 10.574,67 299.900 161.641,38

TOTAL 651.000 394.959,08 62.500 13.363,59 170.500 86.909,21 315.000 126.606,28 - 247,64 83.930 43.546,01 1.282.930 665.631,81



Beneficiary daily rate analysis



Beneficiary daily rate analysis


Project meetings

1st Kick off meeting in DISPAA in Italy in January 2014 Monitoring meeting in DISPAA in Italy in June 2014 2nd Coordination meeting in CEBASCSIC in Murcia (Spain) on July 7th

2014 3rd Coordination meeting in ASTRA in Italy on January 2015 4th Coordination meeting in DISPAA in Italy on June 2015

Next Project meeting 5th Final Coordination meeting in Soldano in Italy on December 2015

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