WELCOME TO MY

PRESENTATION

Presented ByMd. Rezaur Rahman

REGISTRATION NO.: 1305099

SupervisorDr. Md. Faruk Islam Associate Professor

DEPARTMENT OF MEDICINE, SURGERY AND OBSTETRICS HAJEE MOHAMMAD DANESH SCIENCE & TECHNOLOGY

UNIVERSITY, DINAJPUR

SEROPREVALANCE AND EFFICACY OF COMMERCIAL VACCINE AGAINST NEWCASTLE

DISEASE IN LAYER BIRDS

Co SupervisorDr. Md. Shajedur Rahman Assistant Professor

Newcastle Disease is one of the most important infectious disease of poultry in Bangladesh

It is still considered a major problem, because of its high morbidity, mortality particularly in rural areas and reduce productivity and economic loss in layer farm

Velogenic Strain Cause Mortality up to 90% and Morbidity near 100%

Prevalence of ND in different areas of Bangladesh are 50%, 45%, 25% and 32.5%.

BACKGROUND

Determination of seroprevalence of Newcastle Disease (ND) by Hamagglutination Inhibition (HI) test

Evaluation of immune response of different commercial vaccines in grower and layer birds.

OBJECTIVES

MATERIALS & METHODS

Study area: The study was carried out at different layer

farms of three upazilla namely- Kaharol, Bochagonj and Setabgonj in Dinajpur district

Total Number of Sample: 20 blood samples from each farm

Study period: January-June, , 2015

MATERIALS & METHODS

Study farm and population:Study farm and population:

Six (06) farms and total population of birds are 6000

Laboratory examination:

Toufiq Agro Laboratory, RangpurToufiq Agro Laboratory, Rangpur

MATERIALS & METHODS

SL No.

Name of Vaccine Strain Company

01 Avinew® VG/GA Strain Merial

02 Cevac New L® LaSota strain ACI

03 Newcastle Disease vaccine®

Mesogenic strain Novartis

MATERIALS & METHODS

Phosphate Buffer Solution (PBS) Newcastle Disease Vaccine Experimental Chicken Disposable Syringe Micropipette 96 Well micro titre plate Glassware and Appliances Chicken Red Blood Cell (cRBC) Suspension

MATERIALS MATERIALS & METHODS

Fig: Study Flow chart

VACCINES

Select of farms

Collection of 20 blood sample from each farm for Haemagglutination inhibition (HI) test to know Ab

titire

HI perform at Toufic agro lab. To determine the Seroprevalence

Selection of farms for vaccination

EXPERIMENTAL LAYOUT

Vaccination: Farm A --- New Castle Diseases Vaccine (Mesogenic Strain) Farm C --- Avinew (VG/GA Strain) Farm D--- Cevac new-L (Lasota)

20 blood serum from each farm for determination of Ab titre (20 × 3 = 60) at

Day7 and Day14

Evaluation and comparison of vaccine effectiveness against ND

EXPERIMENTAL LAYOUT

RESULTS

Table A. Seroprevalence of Newcastle Disease in grower farms

Ab = Antibody

Farms

Grower birdsOverall

prevalenceAge of birds (wks)

Total serum tested

Mean Ab titre

levelPositive

casePrevalence

among tested cases

A 16 20 23.6 5 25%

18.33%B 12 20 28.9 0 0%

C 14 20 23.9 6 30%

Farms

Layer birds

Overall prevalenceAge of

birds (wks)

Total serum tested

Mean Ab titre

levelPositive

casePrevalence

among tested cases

D 27 20 25.67 35%

23.33%E 22 20 25.84 20%

F 40 20 23.63 15%

Table B Seroprevalence of Newcastle Disease in layer farms

Table C. Ab titre level of 3 farms at Day0 (Day of vaccination)

(HI test at experimental “Day 0” at Geometric mean log2)(C-Mamun poultry, D- Masud poultry, A- Rakib poultry)

Farms StageNo. of blood

serum sample tested

Mean of Titre (Before Vaccination)

Farm-C Grower 20 23.9

Farm-A Layer 20 25.6

Farm-D Grower 20 23.6

Table D. Evaluation of vaccine efficacy after Day 7 and Day 14 day of following vaccination by different vaccine in

different ages layer farms

(Farm C-Mamun poultry, D- Masud poultry, A- Rakib poultry)

Table E. Comparative study of Immune response at grower farms

NS= Not Significance

Farms(grower) Vaccine name HI (D7) titre

After VaccinationHI (D14) titre

After Vaccination

A Avinew (VG/GA strain) 27.6 28.1

CNewcastle disease vaccine (Mesogenic

strain)27.2 28.0

P Value (t-test) NS NS

CONCLUSIONS

Detection of seroprevalance of ND in layer birds may assist to reduced prevalence of ND in layer farm through proper vaccination.

The titre level in farm C (Avinew VG/GA strain), A (Mesogenic strain) and D (Cevacnew-Lasopa) on Day7 and Day14 was 27.2 ,28

; 27.6 , 28.1 and 29.1 , 29.8 , respectively in three farms Cevac New L (LaSota strain) may be the choice of vaccine

against ND for layer farms though others may also be used. Further study may need to conduct in large scale of birds using

all commercial vaccine for recommendation.

THANKS TO ALL