ELSEVIER

Cell Transplantation, Vol. 6. No. 6, pp. xl-xix, 1997 0 I997 Elsevier Science Inc.

Printed in the USA. All right\ rewxd 0963-6897/97 $17.00 + .oo

VOLUME 6, NUMBER I

CONTENTS

ORIGINAL CONTRIBUTIONS

Transfer and Expression of the Interferon Gamma Gene in Human Endothelial Cells Inhibits Vascular Smooth Muscle Cell Growth In Vitro Alison T. Stopeck, Mahmood Vahedian, and Stuart K. Williams

Incorporation of P-Galactosidase-Expressing Endothelial Cells Into the Skeletal Muscle Microvascular Bed of Mice Christoph Schwarzler, Gerald Webersinke, Reinhard Fassler, Alois Lametschwandtner, Hannelore Batter, and Hans-Christian Bauer

Repeated Intraportal Injections of Subtherapeutic Islet Cell Isografts Restore Normoglycemia in Streptozotocin-Diabetic Rats Eugenio Morsiani, Jacek Rozga, Giovanni Dellagiacoma, and Achilles A. Demetriou

1997

9

17

Insulin Secretion and Glucose Tolerance After Islet Transplantation in Rats With Noninsulin-Dependent Diabetes Induced by Neonatal Streptozotocin Maria-Angeles Tormo, Trinidad Leon-Quinto, Catherine Saulnier, Danielle Bailbe, Patricia Serradas, and Bernard Portha 23

Effects of Caffeine and Acetylcholine on Glucose-Stimulated Insulin Release From Islet Transplants in Mice Chun-Liang Shi

Effect of Fusidic Acid on Pancreatic Islet Allograft Rejection Jose F. Mendola, Helena Corominola, Enric Esmatjes, Alex Saenz, Laureano Fernandez-Cruz, and Ramon Gomis

33

39

Development of an Automated Computer-Controlled Islet Isolation System Jonathan R. T. Lakey, Garth L. Warnock, Mark Brierton, Ziliang Ao, Bernhard J. Hering, Nick J. M. London, Camillo Ricordi, Frank Corbin, and Ray V. Rajotte 47

Isolation and Characterization of a Cell Line From the Epithelial Cells of the Human Fetal Pancreas Sijian Wang, Gillian M. Beattie, Martin 1. Mally, Vincenzo Cirulli, Pam Itkin-Ansari, Ana D. Lopez, Albert0 Hayek, and Fred Levine 59

Cyclosporine Does Not Inhibit the Process of Revascularization of Pancreatic Islet Transplantation Jose F. Mendola, Cristian Goity, Enric Esmatjes, Alex Saenz, Laureano Fernandez-Cruz, and Ramon Gomis

Allogenic Hepatocyte Transplantation Using FK 506 P. M. Markus, P. Krause, A. Fayyazi, K. Honnicke, and H. Becker

Intraretrosplenial Cortical Grafts of Fetal Cholinergic Neurons and the Restoration of Spatial Memory Function Ying J. Li and Walter C. Low 8.5

xi

69

77

Contents, Author, and Keyword Index xii

COMMENTARY

First Human Myoblast Transfer Therapy Continues to Show Dystrophin After 6 Years Peter K. Law, Tena G. Goodwin, Qiuwen Fang, Terry L. Hall, Tom Quinley, George Vastagh, Vijaya Duggirala, Charles Larkin, Jerry Ann Florendo, Lawrence Li, Tunja Jackson, T. J. Yoo, Nancy Chase, Michael Neel, Tim Krahn, and Randall Holcomb

MEETINGS

PatentsALERT

95

I

III

VOLUME 6, NUMBER 2 1997

CONTENTS

ORIGINAL CONTRIBUTIONS

Control of Inflammatory Damage by Anti-LFA-1: Increase Success of Myoblast Transplantation Benoit Guerette, Isabelle Asselin, Daniel Skuk, Mark Entman, and Jacques P. Tremblay 101

Hybrid Muscular Tissues: Preparation of Skeletal Muscle Cell-Incorporated Collagen Gels Takahisa Okano and Takehisa Matsuda 109

Immunological Response Against Allogeneic Chondrocytes Transplanted Into Joint Surface Defects in Rats Anna Hyc, Jacek Malejczyk, Anna Osiecka, and Stanislaw Moskalewski 119

Culture Expanded Canine Mesenchymal Stem Cells Possess Osteochondrogenic Potential In Vivo and In Vitro S. Kadiyala, R. G. Young, M. A. Thiede, and S. P. Bruder 125

Metabolic Energy Capacity of Dopaminergic Grafts and the Implanted Striatum in Parkinsonian Nonhuman Primates as Visualized With Cytochrome Oxidase Histochemistry Timothy J. Collier, D. Eugene Redmond, Jr., Robert H. Roth, John D. Elsworth, Jane R. Taylor, and John R. Sladek, Jr.

Cell Proliferation in Retinal Transplants Rajesh K. Sharma and Berndt Ehinger

Transplantation of Retinal Pigment Epithelium Using Viable Cryopreserved Cells Yusuf K. Durlu and Makoto Tamai

135

141

149

Comparison of Methods Used for the Removal of DMSO Following Cryopreservation and the Development of an Automated Protocol Jonathan R. T. Lakey, Garth L. Warnock, and Ray V. Rajotte 163

Freezing Characteristics of Isolated Pig and Human Hepatocytes T. B. Darr and A. Hubel 173

Contents, Author, and Keyword Index Xl,,

Post-Thaw Viability and Functionality of Cryopreserved Rat Fetal Brain Cells Cocultured With Sertoli Cells D. F. Cameron, A. I. Othberg, C. V. Borlongan, S. Rashed, A. Anton, S. Saporta, and P. R. Sanberg 18.5

COMMENTARY

The Testis-Derived Cultured Sertoli Cell as a Natural FAS-L Secreting Cell for Immunosuppressive Cellular Therapy Paul R. Sanberg, Samuel Saporta, Cesario V. Borlongan, Agneta I. Othberg, Richard C. Allen, and Don F. Cameron 191

LETTERS TO THE EDITOR 195

MEETINGS 1

PatentsALERT IV

VOLUME 6, NUMBER 3 1997

CONTENTS

SPECIAL ISSUE: NEURAL TRANSPLANTATION

INTRODUCTION

A Special Issue Based on the Third Annual Conference of the American Society for Neural Transplantation, 1996 William J. Freed and Ole Isacson 201

ORIGINAL CONTRIBUTIONS

Neuropsychological Functioning Following Fetal Striatal Transplantation in Huntington’s Chorea: Three Case Presentations L. M. Philpott, 0. V. Kopyov, A. J. Lee, S. Jacques, C. M. Duma, S. Caine, M. Yang, and K. S. Eagle 203

Fetal Grafting for Parkinson’s Disease: Expression of Immune Markers in Two Patients With Functional Fetal Nigral Implants Jeffrey H. Kordower, Scot Styren, Martha Clarke, Stephen T. DeKosky, C. Warren Olanow, and Thomas B. Freeman 213

Colonization of Neural Allografts by Host Microglial Cells: Relationship to Graft Neovascularization Nathan A. Pennell and Wolfgang J. Streit 221

Growth Properties of Neural Cell Lines Immortalized With the tsA58 Allele of SV40 Large T Antigen M. E. Truckenmiller, Ora Dillon-Carter, Carlo Tornatore, Henrietta Kulaga, Hidetoshi Takashima, and William J. Freed 231

xiv Contents, Author, and Keyword Index

Differential Dissection of the Rat El6 Ventral Mesencephalon and Survival and Reinnervation of the 6-OHDA-Lesioned Striatum by a Subset of Aldehyde Dehydrogenase-Positive TH Neurons Nadia S. K. Haque, Celeste J. LeBlanc, and Ole Isacson 239

Cellular Delivery of Human CNTF Prevents Motor and Cognitive Dysfunction in a Rodent Model of Huntington’s Disease Dwaine F. Emerich, Chris K. Cain, Corinne Greco, Joel A. Saydoff, Zhong Yi Hu, Hanjiu Liu, and Mark D. Lindner 249

Intranigral Grafts of Fetal Ventral Mesencephalic Tissue in Adult 6-Hydroxydopamine-Lesioned Rats Can Induce Behavioral Recovery R. E. Johnston and J. B. Becker 267

Addition of Lateral Ganglionic Eminence to Rat Mesencephalic Grafts Affects Fiber Outgrowth but Does Not Enhance Function Mia Emglrd-Mattson, Jenny Karlsson, Naoyuki Nakao, and Patrik Brundin 277

The Age of Striatum Determines the Pattern and Extent of Dopaminergic Innervation: A Nigrostriatal Double Graft Study Ingrid Stromberg, Lars Bjorklund, and Petter Forander

The Effects of Storage Conditions and Trophic Supplementation on the Survival of Fetal Mesencephalic Cells P. Thajeb, Z. D. Ling, E. D. Potter, and P. M. Carvey

287

297

Comparison of Neurotoxicity Following Repeated Administration of L-DOPA, D-DOPA, and Dopamine to Embryonic Mesencephalic Dopamine Neurons in Cultures Derived From Fisher 344 and Sprague-Dawley Donors T. Alexander, C. E. Sortwell, C. D. Sladek, R. H. Roth, and K. Steece-Collier 309

Engraftment of C6-2B Cells Into the Striatum of AC1 Nude Rats as a Tool for Comparison of the in vitro and in vivo Phenotype of a Glioma Cell Line Carlo Tornatore, Stuart Rabin, Belinda Baker-Cairns, Stuart Keir, and Italo Mocchetti 317

Altered Differentiation of CNS Neural Progenitor Cells After Transplantation Into the Injured Adult Rat Spinal Cord Stephen M. Onifer, Allison B. Cannon, and Scott R. Whittemore 327

MAP2 Expression in the Developing Human Fetal Spinal Cord and Following Xenotransplantation Mark A. Giovanini, Paul J. Reier, Thomas A. E&in, and Douglas K. Anderson 339

Cerebellar Grafts Partially Reverse Amino Acid Receptor Changes Observed in the Cerebellum of Mice With Hereditary Ataxia: Quantitative Autoradiographic Studies Kalliope Stasi, Adamantia Mitsacos, Lazaros C. Triarhou, and Elias D. Kouvelas

Functional Characterization of NGF-Secreting Cell Grafts to the Acutely Injured Spinal Cord Mark H. Tuszynski, Keith Murai, Armin Blesch, Ray Grill, and Ian Miller

347

361

MEETINGS

Contents, Author, and Keyword Index

VOLUME 6, NUMBER 4

CONTENTS

ORIGINAL CONTRIBUTIONS

Use of Allogeneic Bone Marrow Labeled With Neomycin Resistance Gene to Examine Bone Marrow- Derived Chimerism in Experimental Organ Transplantation J. P. Smith, J. Kasten-Jolly, L. Rebellato, Carl E. Haisch, and Judith M. Thomas

Analysis of Hepatocyte Distribution and Survival in Vascular Beds With Cells Marked by 99mTc or Endogenous Dipeptidyl Peptidase IV Activity Sanjeev Gupta, Srinivasa Rao G. Vasa, Pankaj Rajvanshi, Lionel S. Zuckier, Christopher J. Palestro, and Kuldeep K. Bhargava

Alginate Polylysine Microcapsules as Immune Barrier: Permeability of Cytokines and Immunoglobulins Over the Capsule Membrane Bard Kulseng, Beate Thu, Terje Espevik, and Gudmund Skjak-Brzk

Towards the Development of a Bioartificial Pancreas: Effects of Poly-L-Lysine on Alginate Beads With BTC3 Cells J. P. Benson, K. K. Papas, 1. Constantinidis, and A. Sambanis

Histochemical Analysis of the Role of Class I and Class II Clostridium histolyticum Collagenase in the Degradation of Rat Pancreatic Extracellular Matrix for Islet Isolation Greetje H. Vos-Scheperkeuter, Paul T. R. van Suylichem, Maike W. A. Vonk, Gerrit H. J. Walters, and Reinout van Schilfgaarde

Control of Insulin Secretion and Glucose Homeostasis in Exercising Diabetic Rats With Intrasplenic or Kidney Subcapsular Islet Grafts Harmina Houwing, Luchiena G. Hilbrands, Paul T. R. Van Suylichem, Jan E. Bruggink, Anton B. Steffens, and Jan H. Strubbe

Hypoosmotic Exposure of Canine Pancreatic Digest as a Means to Purify Islet Tissue Jonathan R. T. Lakey, Michael A. J. Zieger, Erik J. Woods, Jun Liu, and John K. Critser

Successful Reversal of Diabetes by Single Donor Isologous Islet Transplantation in a Mouse Model C. K. Leow, D. W. R. Gray, and P. J. Morris

A Rapid and Sensitive Cellular Enzyme-Linked Immunoabsorbent Assay (CELISA) for the Detection and Quantitation of Antibodies Against Cell Curface Determinants II. Optimal Reagent Concentrations and Predictive Analysis Garo K. Balkian, Kathleen M. Flores, Ramon Llull, Harry S. Ko, Kenneth W. Walker, Kirby S. Black, and Charles W. Hewitt

An Internet Multicast Symposium Concerning the Microcirculation of the Islets of Langerhans F. Charles Brunicardi, Stefan Moldovan, Stewart Gilman, Edward Livingston, and Edward Passaro, Jr.

BOOK REVIEW

Yearbook of Cell and Tissue Transplantation, edited by Robert P. A. E. Willing and P. R. Sanberg

Lanza and William L. Chick

1997

369

377

387

395

403

413

423

429

431

439

443

xvi Contents, Author, and Keyword Index

LETTER TO THE EDITOR

Jon A. Wolff 445

MEETINGS

PatentsALERT II

VOLUME 6, NUMBER 5 1997

CONTENTS

ORIGINAL CONTRIBUTIONS

Functional Recovery of Porcine Hepatocytes After Hypothermic or Cryogenic Preservation for Liver Support Systems Sharda Naik, Henry A. Santangini, Donna M. Trenkler, Claudy J.-P. Mullon, Barry A. Solomon, Jing Pan, and Hugo 0. Jauregui 447

Selective Intraportal Transplantation of DiI-marked Isolated Rat Hepatocytes P. M. Markus, S. Koenig, P. Krause, and H. Becker 455

Highly Porous Polymer Matrices as a Three-Dimensional Culture System for Hepatocytes P. M. Kaufmann, S. Heimrath, B. S. Kim, and D. J. Mooney 463

Polymer-Encapsulated PC-12 Cells Demonstrate High-Afftnity Uptake of Dopamine in vitro and ‘sF-DOPA Uptake and Metabolism After Intracerebral Implantation in Nonhuman Primates Thyagarajan Subramanian, Dwaine F. Emerich, Roy A. E. Bakay, John M. Hoffman, Mark M. Goodman, Timothy M. Shoup, Gary W. Miller, Allan I. Levey, George W. Hubert, Scott Batchelor, Shelly R. Winn, Joel A. Saydoff, and Ray L. Watts 469

Organization and Histochemical Phenotype of Human Fetal Cerebellar Cells Following Transplantation Into the Cerebellum of Nude Mice L. L. Pundt, E. A. J&n, J. A. Conrad, and W. C. Low

Bone Marrow Mononuclear Cell Count Does Not Predict Neutrophil and Platelet Recovery Following Autologous Bone Marrow Transplant: Value of the Colony-Forming Unit Granulocyte-Macrophage (CFU-GM) Assay Fahad Al-Fiar, H. Miles Prince, Kevin Imrie, A. Keith Stewart, Michael Crump, and Armand Keating

Glucoregulation After Canine Islet Transplantation: Contribution of Insulin Secretory Capacity, Insulin Action, and the Entero-Insular Axis Michael P. M. van der Burg, Paul T. R. van Suylichem, Onno R. Guicherit, Marijke Frolich, Herman H. P. J. Lemkes, and Hein G. Gooszen

CTLA 4-Ig Prolongs Survival of Microencapsulated Neonatal Porcine Islet Xenografts in Diabetic NOD Mice Collin J. Weber, Mary K. Hagler, John T. Chryssochoos, Judith A. Kapp, Gregory S. Korbutt, Ray V. Rajotte, and Peter S. Linsley

479

491

497

505

Contents, Author, and Keyword Index xvii

SPECIAL SECTION

Special Section: Introduction: Cell Therapy ‘96 Toru Itakura 509

Evaluation of Intracerebral Grafting of Dopamine-Secreting PC12 Cells Into Allogeneic and Xenogeneic Brain Takeshi Ono, Isao Date, Takashi Imaoka, Tetsuro Shingo, Tomohisa Furuta, Shoji Asari, and Takashi Ohmoto

Implantation of Xenogeneic Transgenic Neural Plate Tissues Into Parkinsonian Rat Brain Koichi Hara, Koichi Uchida, Atsushi Fukunaga, Shigeo Toya, and Takeshi Kawase

511

515

Efficiency of Neural Differentiation of Mouse P19 Embryonal Carcinoma Cells is Dependent on the Seeding Density Hiroshi Kitani, Hidetoshi Ikeda, Tadao Atsumi, and Rihito Watanabe 521

Transplantation of Xenogeneic Cells Secreting B-Endorphin for Pain Treatment: Analysis of the Ability of Components of Complement to Penetrate Through Polymer Capsules Yasushi Hagihara, Youichi Saitoh, Hiroo Iwata, Takuyu Taki, Shun-Ichiro Hirano, Norio Arita, and Toru Hayakawa

Enzymatic Activity and Expression of Cytochrome P450 LAw Within Intrasplenically Transplanted Fetal Hepatocytes in Spontaneously Hypertensive Rats Kazuya Kato, Junji Kato, W. John B. Hodgson, Nader G. Abraham, Kazuhiko Onodera, Masato Imai, Shinichi Kasai, and Michio Mito

Immunoregulation via Adhesion Molecules in Allogeneic and Xenogenic Hepatocyte Transplantation to Nagase’s Analbuminemic Rats Hitoshi Horimoto, Masumi Nozawa, Norihiro Kokudo, Masatomo Nakao, Shigeki Takahashi, Masayuki Miyasaka, Kenichiro Seino, Hideo Yagita, and Ko Okumura

An Attempt to Add Biological Functions by Genetic Engineering in Order to Produce High- Performance Bioreactor Cells for Hybrid Artificial Liver: Transfection of Glutamine Synthetase Into Chinese Hamster Ovary (CHO) Cell Shin Enosawa, Seiichi Suzuki, Masayuki Fujino, Hiroshi Amemiya, Takeshi Omasa, Shigeo Urayama, Naoko Tanimura, and Kenichi Suga

Subcutaneous Xenotransplantation of Hybrid Artificial Pancreas Encapsulating Pancreatic B Cell Line (MIN6): Functional and Histological Study Yoshiyuki Kawakami, Kazutomo Inoue, Hiroyuki Hayashi, W. J. Wang, Hiroshi Setoyama, Y. J. Gu, Masayuki Imamura, Hiroo Iwata, Yoshito Ikada, Masumi Nozawa, and Jun-Ichi Miyazaki

Prolonged Effect of Troglitazone (CS-045) on Xenograft Survival of Hybrid Artificial Pancreas Yoshiyuki Kawakami, Kazutomo Inoue, Tein Tun, Hiroyuki Hayashi, Hiroshi Setoyama, Y. J. Gu, W. X. Cui, Masayuki Imamura, Hiroo Iwata, and Yoshito Ikada

Microchimerism and Hyporesponsiveness Induced by Intraportal Injection of Donor Spleen Cells in Rats Hirofumi Ota, Mitsukazu Gotoh, Hiroki Ohzato, Keizo Dono, Yutaka Takeda, Koji Umeshita, Masato Sakon, Isamu Nishisho, and Morito Monden

527

531

535

537

541

547

551

XVlll

MEETINGS

PatentsALERT

Contents, Author, and Keyword Index

I

III

VOLUME 6, NUMBER 6

CONTENTS

Commentary

Infectious Issues in Human Fetal Neural Transplantation Douglas A. Holt, G. Michael Nauert, Agneta I. Othberg, Timothy S. Randall, Alison E. Willing, Ray H. Widen, Robert A. Hauser, Paul R. Sanberg, C. Warren Olanow, and Thomas B. Freeman

Original Contributions

Survival of Nigral Grafts Within the Striatum of Marmosets With 6-OHDA Lesions Depends Critically on Donor Embryo Age L. E. Annett, E. M. Tort-es, D. J. Clarke, Y. Ishida, R. A. Barker, R. M. Ridley, H. F. Baker, and S. B. Dunnet L

Proliferative Response of Human T Lymphocytes to Porcine Fetal Brain Cells Thoman Brevig, Erik B. Pedersen, Tom Kristensen, and Jens Zimmer

Microcarrier Enhanced Survival of Human and Rat Fetal Ventral Mesencephalon Cells Implanted in the Rat Striatum Samuel Saporta, Cesario Borlongan, JoAnn Moore, Elizabeth Mejia-Millan, Stacey L. Jones, Patrice Bonness, Timothy S. Randall, Richard C. Allen, Thomas B. Freeman, and Paul R. Sanberg

Evaluation of an Immunoisolation Membrane Formed by Incorporating a Polyvinyl Alcohol Hydrogel Within a Microporous Filter Support Andrew R. Baker, Ronald L. Fournier, Jeffrey G. Sarver, Jennifer L. Long, Peter J. Goldblatt, James M. Homer, and Steven H. Selman

Survival and Function of Syngeneic Rat Islet Grafts Placed Within the Thymus Versus Under the Kidney Capsule Gina R. Rayat, Gregory S. Korbutt, John F. Elliott, and Ray V. Rajotte 597

Follow-Up Study of the Revascularization Process of Purified Rat Islet Beta-Cell Grafts Jose F. Mendola, Ignacio Conget, Jose Maria Manzanares, Helena Corominola, Odette Viiias, Juanjo Barcelo, and Ramon Gomis

Use of Polyethyleneglycol for Porcine Islet Cryopreservation BCnCdicte Monroy, Jiri Honiger, Sylviane Darquy, and Gerard Reach

Electrostatic Endothelial Cell Seeding Technique for Small-Diameter (~6 mm) Vascular Prostheses: Feasibility Testing Gary L. Bowlin and Stanley E. Rittgers

1997

553

557

571

579

585

603

613

623

Contents, Author, and Keyword Index xix

Electrostatic Endothelial Cell Transplantation With Small-Diameter (~6 mm) Vascular Prosteses: A Prototype Apparatus and Procedure Gary L. Bowlin and Stanley E. Rittgers 631

MEETINGS I

PatentsALERT III

LIST OF CONTENTS, AUTHORS, AND KEYWORD INDEX, VOLUME 6, 1997 IX