DEFINICIÓN DEL PROBLEMA
Accurate and non-invasive detection of infertility associated with microbiota imbalances
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Leandro Emanuel Barreiro
CoFounder & CEO of Microgenesis Diagnosis
February 12, 2020
Global Bank 2016,; Lancet 2018; 392: 1995–2051
Fertility rate
4,7 to 2,4%
1960 to 2015
2
30%
INFLAMMATORY ALTERATIONS
Autoimmunity
UNEXPLAINED INFERTILITY
80%
3
200 million people
15%Couples suffer
Infertility
OMS; JA Mirón Canelo - 2002
Deaths from infectious diseases
CRISIS
FOOD
INDUSTRIALIZATION
27,7 to 1,1%
1909 to 1999
World population
6x
4
Dysbiosis
Increased permeability
Inflammation
Autoimmunity
The Microbioma in autoimmune disease, Clin Exp Immunol, 2019
Microbiota - Autoimmunity - Infertility
Fetal programming
5
• The infertility could be the first subclinical manifestation
• Functional study of the human microbiota as a key factor in diseasesand Reproductive Health
NGSBacterial DNA Extraction
MetagenomicsQuantification
Taxonomic profile
Metabolomics
Profile Comparison
Functional analysis
Difference between analysis based on
homologies in METAGENOMICS by functional analysis
Lower costMore scalable
Results inless time
Clinical impactand accurate
6
• But these do not differentiate between live and functional microbiome
• Much less offer precise treatment with clinical impact
"Master Switches"
Effectively regulate and coordinate multiple cell pathways and processes by coordinating the expression of multiple genes in multiple pathologies
Technology
Taqman probes + real-time PCRHigh sensitivity and specificityAvailable in any medium-high complexity laboratory
Advantage
Evaluate gene expressionReproducible resultsHigh tissue specificity among individualsHigh stability in serum and plasma
microRNAs?
7
MicroRNAs can be used as non-invasive
diagnostic molecular biomarkers associated
with microbiota altered parameter
8
L. brevis
L. iners
Dysbiosis(species
abundance)
miRx
miRy
Early and non-invasive study of the infertile patient
8WE DEVELOPED THE FIRST FUNCTIONAL TEST TO STUDY THE MICROBIOTA WITH CLINICAL IMPACT
Importance of treatments
The patient is treated to seek
pregnancyB
iom
ed
ica
l d
iets
Imm
un
om
od
ula
tors
Vit
am
ins
Pro
bio
tics
Am
ino
acid
s
9
Restoring fertility by modulating functionality of the immune system in response of microbiota balance
Workflow
Day 1 Day 7 Day 15
10
We can analysis the metabolites of interest through a low-cost and scalable technology
288 patients showing implantation failure and unexplained infertility from 40 clinics in
Spain and Latin America
75% Markers
autoimmunity
40%dysbiosis
Ourexperie
nce
66% pregnancies
11
Immunological test
40%dysbiosis
75% pregnancies
Immunological test
+
miRNA test
12
12
Main Challenge: Development of diagnostic techniques and integral treatments for the microbiota
communication within couples with infertility and the patients that suffer inflammatory diseases
Dr. Agustina AzpirozMicrobiologist
CTO
Dr. Gabriela GutierrezImmunologist
CSO
Lic. Leandro BarreiroBiotechnologist
CEO
Our team / Game changers
13
Thank you
Accurate and non-invasive detection of infertility associated with microbiota imbalances
Leandro Emanuel Barreiro
CoFounder & CEO of Microgenesis Diagnosis
www.microgenesis.net
