SCAP DBS System on a R80 platform with Stack 4 X 40

System DBS

Patent pending

Dried Blood Spot

Automated sampleextraction platformfor online LC-MS/MSbioanalysis

DBS Integrated Sample Processing

Automated sample processing Advantages

SCAP DBS system components mounted on a robotic platform

Comparison of conventional DBS sample handling with the SCAP DBS system

Dried blood spot (DBS) technology coupled to highly sensitive LC-MS/MS systems opens up new ways in bioanalytics both in the healthcare and the pharmaceutical industries. In the healthcare industry, complete measure-ments are done to diagnose patients to determine the efficacy of administered drugs or to screen for early detection of diseases. In the pharmaceutical industry, measurements are done during the development process of new drugs (e.g. PK/TK studies, etc.).

The major drawback of conventional DBS bio- analytics is the time consuming, error prone and labor intensive nature of manual sample pre-treatment. The SCAP DBS system from Prolab Instruments represents a unique approach for direct and automated DBS analysis and eliminates the drawback of current methods. The SCAP DBS system fully automates the DBS cards handling and allows online sample extraction without the need for punching of sample cards.

Fully automated DBS system sample card handling for LC/MS-MS analysis

Optimized extraction of DBS

Simplified method development by online extraction

Fully automated process saves time and labour costs

No need for additional consumables

Conventional DBS workflow:

Preparing logistics and consumables for offline DBS sample handling.

Load DBS card into SCAP DBS system and start automated process

SCAP DBS systemprocedure

Punching out DBS cards

Pipetting of extraction solvent(s) and internal standard

Extraction / Shaking – Ultra sonic treatment

Centrifugation / Filtration

Injection into HPLC - System

Injection into SCAP HPLC - System

Interchangable clamp adapters allow the extraction of variable area sizes from the DBS

SCAP DBS sample process

The rack with the DBS cards is placed into the SCAP DBS system. According to the software-defined sample list, the robotic arm transfers the card into the clamp module where the DBS card is directly inserted into the HPLC flow path for online extraction without punching out any part of the DBS card. The different adapters available for the SCAP DBS system allow for extraction of different areas of the sample spot and seal the DBS card against pressures of up to 200 bar.

The valve system configuration allows the online addition of internal standard (ISTD) during sample extraction. A SPE pre column is used for online sample cleanup prior to analytical separation on the main column.

Efficient SCAP DBS Workflow

Multiple spot extractionCamera for image analysis

SCAP DBS clamp adapters

Sample card returned to rack

Picks up sample card from SCAP DBS system rack

Places card into SCAP DBS system clamp module

DBS extraction

Phot clamp adapters

Efficient SCAP DBS Workflow

The robotic gripper tool picks up a sample card... ... and places it into the clamp module where it is inserted into the analytical flow path.

Dual valve application example

Column switching configuration with a six and a ten port valve

Clamp Adapters

Analytical Pump

ISTDLoop

WasteWaste

DBS Card

Dilutor ISTD

to MS

Analytical Column

Extraction Pump

21

34

5

6102

1

3

4

5 67

8

9

SPE Column Trap

A BA B

Software

Safety:Automatic detection of proper DBS card, card type, and sample spot

Precision:Automatic centration of sample spot for extraction targeting

Repeatability:Distinct offsets from spot center for up to four extractions of the same sample

Documentation:Complete tracking of samples from card to analytical data:

Card picture before and after extraction

Barcode reading (unique card ID and additional customer barcodes)

Run-time data (timestamps, barcodes, pictures) automatically attached to analytical data, allowing for integrated reports*

The comprehensive and easy-to-use software package includes:

Sample list

Method editor

Run control and detailed status feedback

History viewer

InterfacesExcel™ import and export for sample lists and run-time data

Analytical system (LC-MS/MS) connected by contact closure or software integration*

Flexible integration of customer devices

*depending on analytical data system used

Camera and Image Analysis

Offsets first spot extraction

Allows multiple extractions per spotDetects DBS and centers on the spot

Software

Heinig K, Wirz T, Bucheli F, Gajate-Perez A. Determination of oseltamivir (Tamiflu®) and oseltamivir carboxylate in dried blood spotsusing offline or online extraction. Bioanalysis. 3(4):421-37.

Ganz N, Singrasa M, Nicolas L, Gutierrez M, Dingeman-se J, Döbelin W,Glinski M. Development and validation of a fully automated online human dried blood spot analysis of bosentan and its metabolites using the Sample Card And Prep DBS System. J Chromatogr B Analyt Technol Biomed Life Sci. 15;885-886:50-60.

Prolab GmbH acknowledges all tradenames and trademarks used as the property of their respective owners.

SCAP system® is a registered trademark of Prolab Instruments GmbH, Patent Pending

Camera Card Stack Gripper Clamp Module Valve Module ISTD Dilutor

Kägenstrasse 17CH-4153 ReinachSwitzerland

Tel: +41 61 712 01 28Fax: +41 61 712 01 48Web: www.prolab.che-mail: info@prolab.ch

SCAP DBS sales contact:

Literature