Lillidale DiagnosticsPig Oak FarmHoltWimborneDorsetBh21 7DGEngland
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Test Rapid Brucella Ab Test Kit
A qualitative immunochromatographic assay for the detection of Brucella antibodies in Bovine serum, plasma or whole blood.
20 x Tests/Kit
Rapid Brucella AbTest Kit
Intended use
LilliTest Rapid Brucella Ab Test is a qualitative
immunochromatographic assay for the detection of Brucella
antibodies in Bovine serum, plasma or whole blood. It is only
intended for initial screening and reactive samples should be
confirmed by a supplemental assay such as ELISA.
Principle
LilliTest Rapid Brucella Ab Test works on chromatographic
immunoassay. Basic components of test strip includes: a)
Conjugate pad, which contains Detection Antibody, colloidal gold
conjugated; b) a nitrocellulose membrane strip containing two lines
“T” Brucella abortus LPS and “C” Goat Anti Mouse.
Test sample that is added to the sample well, with adequate amount
of buffer migrates from the sample pad along the conjugate pad
where any IgG present in the sample will bind to the colloidal gold
conjugate. The sample then continues to migrate across the
membrane until it reaches the capture zones where the antibody-
antibody conjugate complex will bind to the immobilized Brucella
abortus LPS antigen (on test line) producing a visible line on the
membrane at “T”. If the respective antibody is not present in the
sample, no reaction occurs in the capture zones and no test line is
formed in the zone corresponding to Brucella LPS antigen. The
sample then migrates further along the strip until it reaches the
control zone, where it produces a second visible line on the
membrane at “C”. This control line indicates that the sample has
migrated across the membrane as intended.
The control line should always appear if the test procedure is
performed properly and the test reagents of control line are
working.
Test kit components
§ 20 aluminum foil pouches each containing one B. abortus Ab
test card and a desiccant.
§ 2 bottles of assay diluent
§ Instruction leaflet
Storage Stability
The kit can be stored at room temperature (2-30 C) or
refrigerated.
The test kit is stable up to the expiration date marked on the
package label.
Do not freeze and do not store the test kit in direct sunlight.
Precautions
§ Treat the specimen as infectious and handle with standard
biosafety measures.
§ Use within 10 minutes after opening pouch.
§ Do not touch result window.
§ Use only the buffer supplied along with the kit.
§ Do not mix components from different kits.
§ Use only for in‐vitro diagnostic purpose.
§ Wear protective gloves while handling specimens. Clean
up spills thoroughly using an appropriate disinfectant.
§ Treat all specimens, used tests and other contaminated
materials as infectious, and dispose accordingly.
§ Do not use with specimen containing precipitates.
Limitation of the test
As with all diagnostic tests the definitive diagnosis should be
based on all data case history, clinical and laboratory findings
etc. being evaluated by the veterinarian.
For more accuracy of immune status, additional follow-up testing
using other laboratory methods is recommended.
°
Specimen Collection & Preparation
§ Blood Specimen: Collect the whole blood using a syringe or
vacutainer into a container containing anticoagulants such as
heparin, EDTA or sodium citrate by venipuncture.
§ Serum: Collect the whole blood using a syringe or vacutainer (NOT
containing anticoagulants such as heparin, EDTA or sodium
citrate) by venipuncture. Leave the syringe or vacutainer,
preferably at an angle, to settle for 30 minutes. Once blood
coagulates, centrifuge the blood to get serum specimen as
supernatant.
§ If the specimen is not used for testing immediately, they should be
refrigerated at 2~8°C.
§ For storage period longer than 5 days, freezing is recommended.
Store at -20°C.
§ The specimen should be brought to room temperature prior to use.
Test Procedure
Allow all kit components and specimen to reach room temperatures
prior to testing.
1.) Take out the test card from the foil pouch and place it on a
horizontal surface.
2.) Add 2 µl of Serum or 5 µl of Whole Blood to the Sample well “S”
3.) When the sample is fully absorbed, add 2 drops of the diluent
provided with the assay to the sample hole.
4.) Wait for 15 minutes and interpret results. The result is considered
invalid after 20 minutes. All results where control band does not
appear are considered invalid.
Interpretation of the results
The presence of two colour bands “T” and “C” within the result window,
no matter which band appears first indicates a positive result.
The presence of only one band at “C” line within the result window
indicates a negative result.
If the control band is not visible within the result window, the result is
considered invalid.